Standard name
Human Ortholog
Description Poly(A)RNA binding protein involved in nuclear mRNA export; component of the nuclear pore; ortholog of human TAP
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.12 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.08 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.15 0.06 0.07 0.09 0.09 0.09 0 0 0 0 – – – 0.11 0.05 0.06 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0.09 0.12 0.08 0 0.05 0 0.07 – – – 0.05 0 0.06 0 0.05 0.05 0.15 0.12 0.08
Golgi 0 0.05 0 0 0.05 0 0 0 0 0 0 0 – – – 0.06 0.1 0.1 0 0 0 0.15 0.12 0
Mitochondria 0 0 0 0 0 0.07 0.07 0 0.24 0.18 0.45 0.29 – – – 0.06 0.06 0.06 0 0 0 0 0.09 0
Nucleus 0 0 0.08 0 0 0 0 0.12 0 0.05 0.05 0.08 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.55 0.22 0.27 0.43 0.47 0.4 0.49 0.32 0.42 0.44 0.43 0.43 – – – 0.2 0.28 0.22 0.86 0.74 0.74 0.19 0.38 0.34
Nucleolus 0.08 0 0 0 0 0.1 0.07 0.11 0.25 0.17 0.19 0.17 – – – 0.05 0.07 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.63 0.87 0.71 0.56 0.51 0.39 0.33 0.37 0.28 0.31 0.23 0.25 – – – 0.71 0.76 0.74 0 0.09 0.05 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 0 0 3 0 0 0 0 1 0 0 – – – 1 3 0 1 1 0 0 0 0
Bud 1 1 0 0 0 0 0 2 0 1 0 5 – – – 0 1 0 2 8 3 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 1 1 1 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 0 0 0 0 0
Cytoplasm 2 9 10 6 11 14 16 19 4 5 4 8 – – – 13 7 7 0 1 1 0 0 1
Endoplasmic Reticulum 5 0 0 4 5 7 8 4 1 2 3 3 – – – 5 6 3 2 3 1 0 0 1
Endosome 5 2 2 3 2 14 21 16 3 8 3 17 – – – 6 3 6 7 17 5 0 0 1
Golgi 1 16 0 1 7 2 2 2 1 0 0 0 – – – 7 14 11 3 2 0 0 0 0
Mitochondria 6 3 0 2 2 11 13 9 27 27 74 69 – – – 7 8 6 0 1 0 0 0 1
Nucleus 2 3 5 2 2 5 6 25 5 8 9 20 – – – 3 3 1 3 15 4 0 0 0
Nuclear Periphery 108 76 18 43 70 61 87 67 48 66 71 103 – – – 24 38 24 194 285 81 0 3 7
Nucleolus 15 5 2 2 5 15 13 22 28 26 31 40 – – – 6 9 5 1 7 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 0 2 0 1 0 3 – – – 0 0 0 0 2 3 0 0 0
Vac/Vac Membrane 125 299 47 57 77 60 58 77 32 47 38 61 – – – 84 103 80 7 36 5 0 0 1
Unique Cell Count 198 343 66 101 150 154 178 208 114 151 164 240 119 135 108 226 386 111 4 8 23
Labelled Cell Count 272 416 84 120 184 190 224 245 150 193 235 330 156 195 144 226 386 111 4 8 23


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 16.0 13.0 11.9 12.5 10.3 10.0 9.8 8.1 8.6 8.0 8.0 – – – 14.0 14.3 13.3 – – –
Std Deviation (1e-4) 2.6 3.1 2.6 2.3 2.2 2.2 2.0 2.0 1.6 1.7 1.6 1.6 – – – 3.3 3.1 3.9 – – –
Intensity Change (Log2) – – – -0.13 -0.05 -0.34 -0.39 -0.41 -0.69 -0.6 -0.71 -0.7 – – – 0.1 0.13 0.03 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm -2.0 -1.8 -1.3 -1.4 -1.4 -2.8 0 0 0 – – – -0.8 -2.4 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 2.1 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 4.3 3.7 6.6 4.9 – – – 0 0 0
Nucleus 0 0 0 0 1.0 0 0 0 0.2 – – – 0 0 0
Nuclear Periphery 2.0 2.7 1.7 3.0 0.8 2.0 2.3 2.3 2.3 – – – -1.1 0.1 -0.8
Nucleolus 0 0 1.7 0 1.9 3.7 2.9 3.1 2.9 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole -1.9 -2.7 -4.4 -5.4 -4.9 -5.6 -5.5 -6.8 -6.9 – – – -0.1 0.8 0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.9828 9.73 9.1479 8.7592 7.1247 8.8707 8.6093 9.9466 8.6363 7.9299 6.5529 8.7189 8.5151 9.9924 9.0313 8.0154 7.0962 9.2457
Actin 0.0098 0.0002 0.0057 0.0017 0.0063 0.0002 0.0081 0.0008 0.0014 0.0037 0.005 0.0007 0.0063 0.0006 0.0014 0.0423 0.0263 0.0005
Bud 0.0006 0 0.0001 0.0001 0.0002 0 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0005 0.0002 0
Bud Neck 0.0005 0.0001 0.0021 0.0001 0.001 0 0.0025 0.0001 0.0005 0.0002 0.0002 0.0001 0.0005 0.0001 0.0006 0.0017 0.0017 0.0001
Bud Periphery 0.0005 0 0.0003 0.0002 0.0004 0 0.0005 0.0001 0.0002 0.0004 0.0003 0 0.0003 0.0001 0.0002 0.0013 0.0003 0
Bud Site 0.0034 0 0.0037 0.0001 0.0002 0 0.0029 0.0001 0.0003 0.0002 0.0004 0 0.0012 0.0001 0.0021 0.0008 0.0006 0
Cell Periphery 0.0003 0 0.0003 0 0.0001 0 0.0002 0 0.0003 0.0001 0.0002 0 0.0001 0 0.0004 0.0004 0.0001 0
Cytoplasm 0.0104 0.0026 0.0013 0.0025 0.0019 0.0002 0.0015 0.0003 0.0012 0.0003 0.0074 0.0003 0.006 0.0008 0.0005 0.0087 0.0024 0.0017
Cytoplasmic Foci 0.0102 0.0001 0.0037 0.001 0.0068 0.0002 0.0097 0.0014 0.002 0.0038 0.0458 0.0005 0.0188 0.0002 0.0024 0.0032 0.0282 0.0008
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0.0008 0.0001 0.0003 0 0.0002 0 0.0001 0.0005 0.0001 0
Endoplasmic Reticulum 0.0042 0.0023 0.0049 0.006 0.0055 0.001 0.007 0.0058 0.0055 0.0147 0.0058 0.0035 0.0076 0.0058 0.0043 0.013 0.0034 0.0019
Endosome 0.0208 0.0007 0.0148 0.0051 0.0181 0.0027 0.0111 0.0122 0.0114 0.0213 0.051 0.0034 0.0305 0.0011 0.0035 0.0239 0.0383 0.0023
Golgi 0.0087 0.0001 0.0036 0.0003 0.0023 0.0011 0.0026 0.0016 0.0008 0.0347 0.0116 0.0012 0.0038 0.0004 0.0012 0.0038 0.0226 0.0017
Lipid Particles 0.0182 0.0003 0.0143 0.0007 0.0204 0.0031 0.0115 0.0029 0.0048 0.0126 0.0777 0.0036 0.0238 0.0045 0.0124 0.0317 0.0275 0.0022
Mitochondria 0.0096 0.0003 0.0018 0.0009 0.0141 0.0002 0.0077 0.0009 0.0046 0.0032 0.0376 0.0004 0.0076 0.008 0.0011 0.0409 0.0046 0.011
None 0.0033 0.0001 0.0004 0.0003 0.0012 0 0.0036 0.0001 0.0006 0.0004 0.0036 0.0001 0.0135 0.0001 0.0002 0.0122 0.0009 0.0001
Nuclear Periphery 0.8515 0.8892 0.8697 0.9013 0.8358 0.9564 0.8832 0.9118 0.8971 0.8622 0.6984 0.9644 0.8066 0.8895 0.8864 0.691 0.7344 0.9496
Nucleolus 0.0031 0.0056 0.0076 0.0011 0.0028 0.0004 0.0011 0.0025 0.0006 0.0004 0.0014 0.0002 0.0027 0.0009 0.0011 0.0039 0.0006 0.0002
Nucleus 0.0113 0.0752 0.0327 0.0386 0.0362 0.0198 0.0076 0.0156 0.0124 0.0103 0.0031 0.0077 0.024 0.0612 0.045 0.097 0.0283 0.0152
Peroxisomes 0.0042 0 0.0015 0.0001 0.0004 0 0.0035 0.0001 0.001 0.0003 0.0238 0.0001 0.0061 0 0.0076 0.0012 0.0459 0.0014
Punctate Nuclear 0.0073 0.0037 0.0023 0.0072 0.0167 0.0021 0.0086 0.012 0.0022 0.0015 0.0081 0.0014 0.0158 0.0018 0.0063 0.0043 0.0208 0.0011
Vacuole 0.0031 0.001 0.0032 0.0021 0.0046 0.0005 0.0036 0.0013 0.0149 0.0026 0.0025 0.0005 0.005 0.0013 0.0023 0.0043 0.0014 0.0008
Vacuole Periphery 0.0188 0.0183 0.026 0.0302 0.0251 0.012 0.0229 0.0302 0.0372 0.027 0.0157 0.0118 0.0194 0.0236 0.0209 0.0133 0.0115 0.0093

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.8761 25.7554 30.0906 39.2945 40.9314 25.1192 31.3049 38.5178 29.7686 39.3365
Translational Efficiency 1.3739 1.693 1.2939 0.9348 1.0235 1.4554 1.2883 0.9066 1.301 1.1154

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1363 1459 335 1458 1367 2525 318 111 2730 3984 653 1569

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1177.85 1117.96 1719.26 1696.44 1151.19 1237.26 1544.45 1807.50 1164.50 1193.57 1634.13 1704.30
Standard Deviation 177.46 221.30 246.92 288.93 179.61 207.39 238.23 324.13 179.04 220.22 257.98 292.95
Intensity Change Log 2 — -0.075287 0.545632 0.526355 — 0.104023 0.423967 0.650870 — 0.016126 0.486777 0.589243

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000328 0.000275 0.000195 0.000082 0.000128 0.000088 0.000361 0.000234 0.000228 0.000157 0.000276 0.000093
Bud Neck 0.000995 0.000733 0.004441 0.002566 0.000534 0.000328 0.002374 0.004636 0.000764 0.000477 0.003434 0.002712
Bud Site 0.000291 0.000307 0.000214 0.000897 0.000112 0.000166 0.000291 0.001190 0.000202 0.000218 0.000251 0.000918
Cell Periphery 0.000064 0.000082 0.000038 0.000055 0.000028 0.000021 0.000061 0.000057 0.000046 0.000043 0.000049 0.000055
Cytoplasm 0.003901 0.006749 0.000252 0.002076 0.002762 0.001585 0.003301 0.000751 0.003331 0.003476 0.001737 0.001983
Cytoplasmic Foci 0.001562 0.001240 0.000311 0.003747 0.000472 0.000942 0.000219 0.008447 0.001016 0.001051 0.000266 0.004080
Eisosomes 0.000004 0.000003 0.000003 0.000002 0.000002 0.000001 0.000003 0.000002 0.000003 0.000002 0.000003 0.000002
Endoplasmic Reticulum 0.006044 0.008598 0.002364 0.001214 0.004580 0.001984 0.003453 0.001209 0.005311 0.004406 0.002894 0.001214
Endosome 0.003572 0.004491 0.000932 0.003751 0.002091 0.002527 0.001555 0.004901 0.002830 0.003246 0.001236 0.003832
Golgi 0.001158 0.001453 0.000268 0.002527 0.000182 0.000906 0.000172 0.001300 0.000669 0.001106 0.000221 0.002440
Lipid Particles 0.003592 0.002675 0.000405 0.003510 0.002297 0.001851 0.000320 0.005139 0.002943 0.002153 0.000363 0.003625
Mitochondria 0.003148 0.003036 0.000887 0.001066 0.001544 0.002028 0.002196 0.000520 0.002345 0.002397 0.001525 0.001028
Mitotic Spindle 0.009596 0.011213 0.039221 0.090516 0.011572 0.009796 0.045475 0.168687* 0.010585 0.010315 0.042266 0.096046
None 0.001576 0.001894 0.000107 0.000192 0.000719 0.000591 0.000947 0.000126 0.001147 0.001068 0.000516 0.000188
Nuclear Periphery 0.337631* 0.348084* 0.656486* 0.364754* 0.308048* 0.422160* 0.543862* 0.365230* 0.322817* 0.395032* 0.601640* 0.364788*
Nuclear Periphery Foci 0.588346* 0.578180* 0.275516* 0.504035* 0.648028* 0.536763* 0.332870* 0.422503* 0.618231* 0.551931* 0.303446* 0.498267*
Nucleolus 0.009377 0.005272 0.005738 0.001542 0.004224 0.001788 0.009980 0.000317 0.006797 0.003064 0.007804 0.001456
Nucleus 0.019310 0.016728 0.009608 0.001956 0.008641 0.008092 0.049024 0.002675 0.013968 0.011255 0.028803 0.002007
Peroxisomes 0.000135 0.000135 0.000142 0.000161 0.000074 0.000074 0.000181 0.000079 0.000104 0.000096 0.000161 0.000155
Vacuole 0.003843 0.004784 0.001506 0.010040 0.002029 0.004689 0.002449 0.009951 0.002935 0.004724 0.001965 0.010034
Vacuole Periphery 0.005527 0.004066 0.001368 0.005307 0.001934 0.003620 0.000905 0.002044 0.003728 0.003783 0.001142 0.005076

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.64 1.25 2.23 4.20 2.60 1.75 -5.05 -1.01 -1.12 0.08 1.40 -1.63 1.70 0.80 4.34
Bud Neck 1.49 -4.98 -5.26 -6.72 2.14 1.41 -5.67 -2.44 -2.55 -0.76 2.48 -7.31 -6.82 -8.02 1.72
Bud Site 0.00 0.98 -3.02 -3.01 -3.25 -1.30 -3.37 -2.11 -2.03 -1.57 -0.24 -2.27 -3.62 -3.59 -3.11
Cell Periphery -1.38 2.47 0.04 1.40 -2.41 1.44 -2.22 -1.79 -2.04 -0.18 0.50 -0.89 -2.34 -2.67 -0.77
Cytoplasm -1.90 4.36 1.50 3.43 -4.70 1.17 -1.27 1.14 0.24 2.31 -0.19 1.41 1.22 1.63 -0.29
Cytoplasmic Foci 0.82 3.70 -3.09 -3.56 -4.72 -2.05 1.26 -1.52 -1.44 -1.55 -0.11 3.90 -4.08 -4.06 -4.92
Eisosomes 1.09 2.03 1.31 0.58 -0.20 1.57 -3.64 -0.84 -1.01 0.51 2.60 -1.72 -0.07 -1.18 1.03
Endoplasmic Reticulum -2.82 6.21 8.61 9.85 3.96 6.35 0.97 1.72 -0.03 1.29 2.03 5.08 10.83 9.28 5.85
Endosome -1.27 5.76 -0.58 0.77 -6.35 -1.00 0.92 -2.19 -1.84 -2.41 -1.05 4.96 -2.42 -1.43 -5.82
Golgi -0.45 2.95 -2.41 -1.53 -4.69 -2.99 -0.58 -2.78 -1.14 -2.65 -1.46 2.69 -3.78 -2.67 -4.90
Lipid Particles 1.42 6.60 -0.06 -1.21 -4.78 0.61 3.63 -1.04 -1.20 -1.72 1.56 7.17 -1.11 -2.14 -5.09
Mitochondria 0.11 3.72 2.85 2.06 -0.75 -0.73 -1.30 0.68 1.57 2.05 -0.13 1.21 2.14 2.38 1.22
Mitotic Spindle -0.67 -4.25 -14.30 -13.79 -5.32 0.54 -5.38 -5.68 -5.74 -3.84 -0.02 -6.81 -15.29 -15.40 -6.12
None -0.72 4.85 4.46 6.02 -1.50 0.43 -1.00 2.59 2.29 2.72 0.34 2.35 5.06 5.60 2.22
Nuclear Periphery -1.17 -22.47 -2.46 -1.28 20.58 -14.39* -12.59 -1.83 2.36 5.24 -12.08 -23.68 -4.71 4.56 18.71
Nuclear Periphery Foci 1.02 27.11* 9.38 8.29 -19.19 13.61* 23.23* 8.93 4.74 -3.28 10.70 35.18 15.50 7.66 -19.03
Nucleolus 2.69 1.71 4.95 3.47 2.83 2.77 -3.62 4.84 4.74 5.78 4.30 -1.48 5.12 2.01 5.38
Nucleus 0.83 2.68 6.12 6.64 3.30 -0.02 -6.02 0.45 0.46 5.71 1.34 -4.55 6.75 6.84 7.05
Peroxisomes 0.06 -0.32 -1.05 -1.04 -0.85 -0.03 -2.70 -0.23 -0.19 1.84 0.34 -2.32 -1.39 -1.46 -0.46
Vacuole -0.92 3.20 -5.00 -4.25 -7.13 -2.83 -1.56 -2.02 -1.60 -1.46 -2.52 -0.42 -6.20 -4.73 -5.01
Vacuole Periphery 1.32 4.52 0.09 -1.43 -5.61 -2.64 2.38 -0.36 2.09 -2.34 -0.10 5.08 -1.78 -1.81 -6.01
Standard name
Human Ortholog
Description Poly(A)RNA binding protein involved in nuclear mRNA export; component of the nuclear pore; ortholog of human TAP
Localization
Cell Percentages nuclear periphery (94%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mex67

Mex67


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mex67-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available