Standard name
Human Ortholog
Description UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.96 0.98 0.96 0.93 0.93 0.8 0.81 – 0.71 0.6 0.57 0.94 0.95 0.99 – 0.98 0.97 0.44 0.79 0.72 0.67 0.7 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.08 0.07 0.25 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.05 0 0.05 0 0.16 0.37 0.29 – 0.42 0.59 0.59 0 0 0 – 0 0 0.07 0.06 0.11 0.09 0.09 0.09
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0.08 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 – 0 0 2 2 7 3 1 4
Bud 0 2 3 0 1 0 2 1 – 1 5 6 0 0 0 – 0 0 0 4 1 4 3 2
Bud Neck 0 0 2 0 1 0 0 1 – 1 0 2 0 1 0 – 7 0 0 2 2 2 2 3
Bud Site 0 0 0 0 0 0 0 0 – 0 2 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 3 1 1 2 3 – 4 1 2 1 1 1 – 4 8 2 1 3 3 1 2
Cytoplasm 58 231 223 126 117 174 160 181 – 142 159 167 84 84 120 – 451 281 56 186 172 82 106 106
Endoplasmic Reticulum 1 2 1 1 4 2 0 1 – 1 1 7 0 0 1 – 37 21 30 2 8 5 5 2
Endosome 2 0 0 1 1 1 0 0 – 3 3 2 1 0 0 – 1 2 5 5 3 1 1 1
Golgi 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 1 1 0 2 1 0
Mitochondria 6 11 5 7 3 31 74 65 – 84 157 172 2 2 0 – 15 7 9 13 26 11 13 13
Nucleus 1 3 0 1 0 0 0 0 – 0 0 1 1 1 0 – 1 3 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 0 1 – 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 1 1 0 0 0 0 0 0 – 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 – 1 0 0 0 0 1 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 0 0 1 – 2 6 6 0 0 0 – 0 0 0 1 2 0 1 1
Vac/Vac Membrane 1 0 0 0 1 2 1 2 – 2 3 2 1 2 0 – 2 1 9 5 3 1 5 4
Unique Cell Count 67 241 227 131 126 188 199 224 199 264 293 89 88 121 460 290 126 238 239 123 152 149
Labelled Cell Count 71 252 235 139 130 211 241 257 242 338 367 90 91 123 518 323 126 238 239 123 152 149


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.0 3.7 3.6 3.6 3.2 2.9 3.3 – 2.9 3.0 2.8 5.3 5.3 5.3 4.3 5.1 5.7 5.0 5.9 6.4
Std Deviation (1e-4) 0.9 1.0 1.2 0.9 0.8 1.0 0.9 1.3 – 0.7 1.4 0.9 1.4 1.4 0.7 1.1 1.4 1.5 1.7 2.4 3.2
Intensity Change (Log2) – – – -0.01 -0.03 -0.21 -0.35 -0.13 – -0.34 -0.26 -0.38 0.53 0.53 0.55 0.22 0.47 0.64 0.46 0.69 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5293 0.2146 0.7666 -0.1039 0.8384 0.564 2.5114 1.8784 2.289 2.2713 2.3017 2.1726 1.8236 1.9937 1.5819 2.0437 1.7681 1.7809
Actin 0.0627 0.0011 0.0008 0.0004 0.005 0.0003 0.0455 0.0005 0.0074 0.0002 0.0346 0.0046 0.0312 0.0001 0.0144 0.0002 0.0211 0.0049
Bud 0.001 0.0012 0.0003 0.0001 0.006 0 0.0007 0.0031 0.0007 0.002 0.0008 0.0002 0.0003 0 0.0023 0 0.0003 0.0001
Bud Neck 0.015 0.0002 0 0.0001 0.0011 0.0005 0.0151 0.0002 0.0002 0.0001 0.0004 0.0004 0.003 0.0001 0.0002 0 0.0005 0.0001
Bud Periphery 0.0018 0.0008 0.0005 0.0001 0.0118 0 0.0013 0.0055 0.001 0.0004 0.001 0.0009 0.0009 0 0.0052 0 0.0005 0.0002
Bud Site 0.0197 0.0127 0.0001 0 0.0631 0 0.0218 0.0088 0.0021 0.0004 0.0019 0.0001 0.0033 0.0006 0.0021 0 0.001 0.0002
Cell Periphery 0.0018 0.0003 0.0001 0.0001 0.0026 0.0001 0.0008 0.0004 0.0003 0.0002 0.0001 0.0001 0.0004 0.0002 0.0003 0.0001 0.0002 0
Cytoplasm 0.0442 0.0409 0.0599 0.0167 0.1027 0.0552 0.0849 0.0961 0.1637 0.1955 0.1581 0.2082 0.1391 0.1264 0.139 0.162 0.1873 0.1393
Cytoplasmic Foci 0.0242 0.0109 0.0051 0.0041 0.0074 0.002 0.0287 0.0063 0.0141 0.0121 0.0178 0.0187 0.0102 0.0025 0.0104 0.0046 0.0038 0.004
Eisosomes 0.0017 0.0006 0.0004 0.0003 0.0004 0.0001 0.0003 0.0001 0.0004 0.0001 0.0005 0.0001 0.0005 0.0002 0.0007 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.005 0.0012 0.0034 0.0012 0.0017 0.0019 0.0087 0.0018 0.0058 0.0097 0.0021 0.0074 0.0113 0.0019 0.0034 0.0045 0.0031 0.0022
Endosome 0.0259 0.001 0.0021 0.0006 0.0224 0.0003 0.0274 0.0021 0.0071 0.0024 0.004 0.0075 0.0072 0.0006 0.0015 0.0027 0.0045 0.0036
Golgi 0.0101 0.0002 0.0003 0.0001 0.0043 0 0.0044 0.0002 0.009 0.0001 0.0066 0.0043 0.0024 0.0001 0.0009 0.0001 0.0018 0.0018
Lipid Particles 0.0225 0.0007 0.001 0.0014 0.0303 0.0001 0.0078 0.0011 0.0034 0.0005 0.0027 0.0109 0.005 0.0002 0.0024 0.0004 0.0019 0.0016
Mitochondria 0.012 0.0003 0.0006 0.0002 0.0456 0.0001 0.0021 0.0004 0.0155 0.0144 0.0221 0.0044 0.0012 0.0001 0.002 0.0002 0.0011 0.0085
None 0.644 0.9235 0.9205 0.9726 0.6287 0.9381 0.6966 0.8698 0.7559 0.7538 0.7356 0.7133 0.7623 0.8649 0.7957 0.8229 0.7652 0.8291
Nuclear Periphery 0.025 0.0004 0.0012 0.0008 0.0134 0.0002 0.0206 0.0005 0.0016 0.001 0.0004 0.0021 0.0043 0.0003 0.0008 0.0006 0.0036 0.0004
Nucleolus 0.0035 0.0003 0.0002 0.0001 0.0013 0 0.0009 0.0003 0.0005 0.0025 0.0004 0.0001 0.001 0.0001 0.0002 0.0001 0.0002 0
Nucleus 0.0196 0.0007 0.0007 0.0002 0.0022 0.0004 0.0067 0.0006 0.0014 0.0021 0.0005 0.0007 0.002 0.0009 0.0005 0.0005 0.001 0.0004
Peroxisomes 0.0173 0.0007 0.0007 0.0003 0.0414 0.0001 0.0061 0.0002 0.0051 0.0005 0.0077 0.0126 0.0064 0 0.0143 0.0001 0.0003 0.0021
Punctate Nuclear 0.0381 0.0016 0.0007 0.0004 0.0024 0.0002 0.0075 0.001 0.0036 0.0006 0.002 0.0014 0.006 0.0003 0.0029 0.0005 0.0011 0.0004
Vacuole 0.0034 0.0007 0.001 0.0003 0.0033 0.0003 0.0081 0.0012 0.001 0.0012 0.0004 0.0012 0.0016 0.0006 0.0005 0.0004 0.0006 0.0005
Vacuole Periphery 0.0015 0.0001 0.0002 0.0001 0.003 0 0.0043 0.0001 0.0004 0.0003 0.0002 0.0005 0.0004 0 0.0001 0.0001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.149 7.2581 10.7717 12.2182 11.5796 8.1147 12.1088 12.6304 11.8932 15.5829
Translational Efficiency 0.8246 0.768 0.5046 0.424 0.6565 0.689 0.5416 0.3484 0.4569 0.5056

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
102 1159 207 848 997 1084 2264 1125 1099 2243 2471 1973

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 869.60 719.60 897.66 822.31 626.51 711.23 824.50 791.13 649.07 715.55 830.63 804.53
Standard Deviation 127.93 133.10 97.71 126.90 73.41 88.96 113.29 120.92 106.69 114.00 113.89 124.49
Intensity Change Log 2 — -0.273157 0.045817 -0.080670 — 0.182979 0.396182 0.336577 — -0.064364 0.203003 0.108924

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006384 0.000107 0.000456 0.006323 0.000251 0.001289 0.000184 0.001628 0.000820 0.000678 0.000207 0.003646
Bud Neck 0.002242 0.014266 0.003245 0.009199 0.021937 0.046826 0.005510 0.011593 0.020109 0.030001 0.005320 0.010564
Bud Site 0.026789 0.008767 0.012021 0.034903 0.003204 0.016614 0.006929 0.041736 0.005393 0.012559 0.007356 0.038799
Cell Periphery 0.000823 0.000395 0.000403 0.000364 0.000193 0.000236 0.000223 0.000337 0.000251 0.000318 0.000238 0.000349
Cytoplasm 0.230581* 0.449060* 0.266048* 0.423498* 0.444561* 0.391808* 0.496626* 0.479446* 0.424701* 0.421391* 0.477310* 0.455400*
Cytoplasmic Foci 0.064225 0.137845 0.038062 0.039073 0.267773* 0.261604* 0.020502 0.037572 0.248882* 0.197655* 0.021973 0.038217
Eisosomes 0.000216 0.000462 0.000135 0.000119 0.000393 0.000240 0.000052 0.000097 0.000377 0.000355 0.000059 0.000107
Endoplasmic Reticulum 0.005955 0.001955 0.025616 0.005859 0.000931 0.001296 0.012126 0.003205 0.001397 0.001636 0.013256 0.004346
Endosome 0.037150 0.011847 0.010083 0.016007 0.011588 0.026697 0.002447 0.005402 0.013960 0.019024 0.003087 0.009960
Golgi 0.025273 0.001195 0.000174 0.011431 0.003946 0.007080 0.000114 0.002234 0.005925 0.004039 0.000119 0.006187
Lipid Particles 0.003271 0.009019 0.009137 0.003435 0.015979 0.010901 0.003275 0.004003 0.014799 0.009928 0.003766 0.003759
Mitochondria 0.041855 0.004481 0.000726 0.014003 0.005101 0.019346 0.000450 0.004855 0.008512 0.011665 0.000473 0.008787
Mitotic Spindle 0.002625 0.004918 0.009169 0.058113 0.000414 0.003896 0.004392 0.008462 0.000619 0.004424 0.004792 0.029802
None 0.000631 0.032880 0.002348 0.008862 0.002264 0.001603 0.003297 0.007987 0.002112 0.017764 0.003217 0.008363
Nuclear Periphery 0.001123 0.000913 0.009757 0.002058 0.000768 0.000692 0.007166 0.001383 0.000801 0.000806 0.007383 0.001673
Nuclear Periphery Foci 0.002055 0.000853 0.016061 0.004236 0.000302 0.000382 0.007285 0.002433 0.000465 0.000626 0.008020 0.003208
Nucleolus 0.000413 0.005193 0.000934 0.000813 0.001020 0.001434 0.000366 0.000561 0.000963 0.003376 0.000413 0.000669
Nucleus 0.145949* 0.248318* 0.455451* 0.192203* 0.153618* 0.109501 0.366535* 0.272707* 0.152906* 0.181230* 0.373983* 0.238106*
Peroxisomes 0.003061 0.004077 0.001006 0.004483 0.011089 0.016842 0.000277 0.002987 0.010344 0.010246 0.000338 0.003630
Vacuole 0.371751* 0.061279 0.138827 0.161449 0.053493 0.078528 0.062086 0.109631 0.083031 0.069615 0.068514 0.131902
Vacuole Periphery 0.027627 0.002171 0.000341 0.003569 0.001176 0.003187 0.000159 0.001741 0.003631 0.002662 0.000174 0.002527

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.10 1.65 -0.48 -2.78 -2.33 -1.46 0.46 -1.94 0.09 -2.00 -0.70 1.65 -2.87 -1.99 -3.26
Bud Neck -9.10 -2.55 -5.53 4.27 -3.88 -6.78 8.21 5.76 11.17 -2.98 -4.10 8.08 6.19 11.52 -3.28
Bud Site 0.42 0.19 -1.35 -3.41 -1.91 -7.76 -2.98 -7.55 -3.23 -6.42 -6.56 -2.30 -8.96 -4.83 -7.45
Cell Periphery -0.47 -0.53 0.70 2.28 3.84 -0.61 -0.79 -2.89 -0.32 -2.09 -2.26 -0.86 -2.08 1.38 -1.33
Cytoplasm -10.42 -1.50 -5.23 9.46 -4.77 4.53 -0.77 3.60 -0.87 4.87 0.15 -1.30 5.08 5.80 7.60
Cytoplasmic Foci -15.14 0.11 1.35 21.25 1.48 0.71 35.33 33.00 32.84 -5.08 6.16 33.55 32.02 37.22 -5.09
Eisosomes -1.53 0.24 2.29 1.78 2.85 4.33 10.49 9.20 8.73 -7.88 0.20 10.49 9.13 2.47 -7.30
Endoplasmic Reticulum 2.88 -3.69 -1.12 -6.01 3.48 -0.96 -9.78 -7.08 -4.68 6.74 -1.22 -10.38 -9.60 -7.41 6.84
Endosome 0.25 0.67 1.09 2.58 1.28 -7.37 9.86 7.32 12.13 -3.02 -5.21 9.18 5.53 11.37 -4.53
Golgi 1.33 1.56 0.60 -2.86 -3.82 -2.14 6.03 2.98 4.05 -1.75 0.27 5.82 1.79 1.63 -4.12
Lipid Particles -6.50 -4.62 -0.57 6.76 4.61 2.97 8.94 8.45 6.82 -0.38 3.38 8.52 8.67 9.39 0.71
Mitochondria 0.81 1.23 0.66 -0.62 -2.61 -5.57 7.19 1.69 6.04 -2.13 -3.61 5.13 1.10 4.56 -3.42
Mitotic Spindle -0.41 -0.71 -5.88 -5.88 -3.54 -2.96 -4.33 -4.29 -2.38 -1.66 -3.52 -4.21 -8.02 -6.14 -5.44
None -7.96 -3.81 -4.14 6.55 -2.96 1.34 -1.55 -2.88 -3.63 -2.36 -7.30 -1.83 -3.93 5.39 -3.26
Nuclear Periphery 4.12 -2.24 -5.35 -10.91 1.71 0.21 -8.51 -5.09 -5.47 6.84 0.16 -8.77 -7.04 -10.97 6.47
Nuclear Periphery Foci 2.41 -4.50 -2.79 -3.97 3.57 -0.27 -7.85 -5.96 -6.14 5.13 -1.01 -8.82 -6.55 -6.18 4.98
Nucleolus -5.61 -3.86 -2.92 5.19 1.24 -0.98 2.54 2.04 2.71 -1.92 -4.53 2.35 1.65 5.83 -2.67
Nucleus -1.94 -7.83 -1.29 1.50 9.72 6.26 -30.98 -15.42 -20.65 9.43 -3.62 -31.40 -14.53 -12.00 15.67
Peroxisomes -3.41 0.52 -1.09 2.29 -1.72 -3.31 9.06 6.66 10.42 -2.05 -0.04 9.05 6.99 9.62 -3.10
Vacuole 13.05 8.57 5.46 -22.42 -6.63 -5.17 -7.93 -17.40 -13.78 -12.95 3.67 -0.55 -17.42 -25.44 -20.55
Vacuole Periphery 1.07 1.42 1.20 1.02 -2.26 -3.21 3.97 0.34 3.08 -2.08 -1.26 3.00 0.95 2.95 -3.25
Standard name
Human Ortholog
Description UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
Localization
Cell Percentages cytoplasm (20%), vacuole (4%), mixed (27%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spt14

Spt14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available