Standard name
Human Ortholog
Description Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.05 0 0 0.07 0 0.05 0 0 0
Golgi 0 0.09 0 0 0 0 0 0 0 0 0 0.07 0.09 0.12 0.38 0.41 0.05 0.07 0 0.05 0.06 0.07
Mitochondria 0.85 0.88 0.96 0.95 0.84 0.86 0.86 0.95 0.91 0.93 0.94 0.87 0.86 0.81 0.19 0.06 0.71 0.81 0.75 0.75 0.73 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.05 0.05 0.06 0.09 0.14 0.1 0.14 0.13 0.1 0.14 0.1 0.06 0.06 0 0.12 0 0 0 0 0 0
Nucleolus 0.06 0 0.11 0.07 0.12 0.16 0.14 0.14 0.14 0.11 0.12 0.06 0 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0.07 0.05 0 0 0.06 0 0 0.11 0.05 0.06 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0 0 0.06 0 0 0 0 0 0 0.11 0.08 0.12 0.12 0.06 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 0 0 1 0 1 0 0 0 0 2 3 1 1 0 4 5 1 2 3 13
Bud 10 1 7 9 0 5 6 4 5 8 10 1 0 0 2 2 2 1 0 1 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2
Bud Site 1 0 3 1 0 2 1 4 4 0 2 0 0 0 0 0
Cell Periphery 3 6 3 4 2 3 1 0 4 1 1 3 8 8 1 1 0 0 0 0 0 0
Cytoplasm 16 1 4 6 3 6 6 3 2 3 2 0 4 8 4 2 11 0 0 0 0 0
Endoplasmic Reticulum 3 2 2 3 3 2 2 3 3 3 2 0 1 1 0 1 0 0 0 0 0 0
Endosome 7 1 1 0 13 5 5 1 0 0 1 8 14 13 0 0 27 2 10 3 3 9
Golgi 2 19 0 2 1 1 0 1 0 0 0 15 27 31 6 7 18 18 6 7 10 17
Mitochondria 318 193 202 244 206 274 323 327 258 300 298 186 256 216 3 1 268 205 159 111 127 153
Nucleus 4 0 0 1 0 1 3 0 1 2 0 0 0 0 0 0 2 0 0 1 0 0
Nuclear Periphery 17 11 10 15 23 43 36 49 38 32 43 22 17 16 0 2 2 1 1 2 2 2
Nucleolus 21 6 23 19 29 52 53 49 39 36 38 13 7 15 0 0 2 2 2 3 3 4
Peroxisomes 8 8 5 9 13 4 5 3 3 3 4 14 14 9 0 1 13 4 22 7 10 16
SpindlePole 2 1 1 1 2 0 4 4 4 1 0 2 4 2 0 0 3 0 1 0 1 1
Vac/Vac Membrane 59 37 8 5 15 10 15 7 5 7 8 24 24 31 2 1 19 8 1 4 7 8
Unique Cell Count 372 220 210 258 244 317 377 344 284 322 317 215 297 266 16 17 380 254 214 149 175 236
Labelled Cell Count 474 288 269 319 311 408 461 455 366 396 409 290 379 351 19 18 380 254 214 149 175 236


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 13.0 7.2 8.7 8.7 8.0 8.5 7.8 7.9 8.0 7.8 10.3 10.8 10.1 9.6 10.5 11.6 12.8 12.6
Std Deviation (1e-4) 2.0 2.6 1.9 2.9 2.2 2.2 2.8 2.7 2.7 2.5 2.6 2.1 2.3 2.3 2.3 2.8 2.9 3.2 3.1
Intensity Change (Log2) 0.27 0.28 0.15 0.24 0.13 0.14 0.16 0.13 0.52 0.59 0.49 0.43 0.55 0.69 0.83 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.1 0 0 0 0 -1.1 -0.6 -0.1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.8 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.0 0 0 0 0 0 0 0 2.8 2.8 0 0
Golgi 0 0 0 0 0 0 0 0 3.9 4.5 5.1 0 0
Mitochondria -0.8 -4.1 -3.7 -4.0 -0.6 -2.3 -1.5 -1.1 -3.5 -3.8 -5.0 -10.3 -11.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.5 1.9 3.3 2.1 3.5 3.2 2.2 3.3 2.1 0.5 0.6 0 0
Nucleolus -1.4 0.3 1.8 1.1 1.1 0.9 0.1 0.4 -1.8 -4.0 -2.1 0 0
Peroxisomes 0.7 1.6 0 0 0 0 0 0 2.1 1.4 0.6 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 1.1 -0.4 0.1 -1.2 -1.4 -1.1 -0.8 2.9 1.9 3.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.2586 31.4801 20.7641 41.3046 35.5116 43.0168 49.5125 38.1916 41.7403 44.3089
Translational Efficiency 2.0642 1.8567 2.9266 1.0534 1.8543 2.4728 1.6742 2.5411 2.3624 2.2463

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
330 958 141 246 1483 1451 17 393 1813 2409 158 639

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 908.81 980.91 1219.65 1069.28 894.30 940.97 1255.54 1047.27 896.94 956.85 1223.51 1055.74
Standard Deviation 139.33 179.30 213.81 161.83 154.86 164.94 286.75 175.55 152.26 171.91 223.09 170.73
Intensity Change Log 2 0.110142 0.424417 0.234589 0.073390 0.489477 0.227803 0.092031 0.457052 0.231227

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001304 0.006144 0.004020 0.005067 0.001520 0.004439 0.091869 0.002107 0.001481 0.005117 0.013472 0.003246
Bud Neck 0.002143 0.001465 0.014975 0.016468 0.000993 0.001306 0.009695 0.009688 0.001203 0.001369 0.014407 0.012299
Bud Site 0.002838 0.004952 0.011919 0.013745 0.001757 0.003439 0.083022 0.016763 0.001954 0.004040 0.019570 0.015601
Cell Periphery 0.000207 0.000277 0.000276 0.000369 0.000279 0.000320 0.001546 0.000199 0.000266 0.000303 0.000413 0.000264
Cytoplasm 0.000273 0.000646 0.001265 0.001359 0.000283 0.001408 0.061702 0.001812 0.000281 0.001105 0.007768 0.001638
Cytoplasmic Foci 0.019055 0.028514 0.090522 0.060045 0.009104 0.029773 0.054086 0.056081 0.010915 0.029272 0.086602 0.057607
Eisosomes 0.000072 0.000127 0.000134 0.000143 0.000087 0.000097 0.000617 0.000124 0.000084 0.000109 0.000186 0.000132
Endoplasmic Reticulum 0.000465 0.000353 0.001543 0.000821 0.000287 0.000371 0.013887 0.000444 0.000319 0.000364 0.002871 0.000589
Endosome 0.024263 0.031465 0.064115 0.075990 0.012572 0.041947 0.088590 0.052266 0.014700 0.037779 0.066748 0.061399
Golgi 0.018919 0.044915 0.056772 0.030139 0.013205 0.045785 0.049398 0.040059 0.014245 0.045439 0.055979 0.036240
Lipid Particles 0.009944 0.011586 0.024329 0.010192 0.008240 0.007418 0.019582 0.007901 0.008550 0.009075 0.023818 0.008783
Mitochondria 0.877977* 0.816430* 0.547004* 0.608209* 0.926239* 0.819717* 0.323283* 0.661901* 0.917455* 0.818410* 0.522933* 0.641231*
Mitotic Spindle 0.006279 0.003368 0.086866 0.054892 0.000756 0.003661 0.058211 0.032695 0.001762 0.003545 0.083783 0.041241
None 0.000524 0.000456 0.000649 0.000481 0.000451 0.000351 0.001489 0.000438 0.000464 0.000393 0.000739 0.000455
Nuclear Periphery 0.001693 0.000221 0.004319 0.003635 0.000315 0.000184 0.000638 0.000811 0.000566 0.000199 0.003923 0.001898
Nuclear Periphery Foci 0.002486 0.000366 0.007201 0.004127 0.000416 0.000309 0.002804 0.001346 0.000793 0.000332 0.006728 0.002417
Nucleolus 0.001227 0.000343 0.002482 0.003693 0.000612 0.000273 0.000321 0.002090 0.000724 0.000301 0.002250 0.002707
Nucleus 0.000517 0.000564 0.002213 0.002721 0.000556 0.000241 0.000238 0.000993 0.000549 0.000369 0.002001 0.001658
Peroxisomes 0.014909 0.036512 0.034441 0.073344 0.012886 0.021911 0.018837 0.085243 0.013254 0.027718 0.032762 0.080662
Vacuole 0.007714 0.005377 0.025975 0.023089 0.004263 0.008721 0.067621 0.015740 0.004891 0.007391 0.030456 0.018569
Vacuole Periphery 0.007192 0.005918 0.018980 0.011472 0.005179 0.008331 0.052565 0.011298 0.005545 0.007372 0.022593 0.011365

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.51 -2.18 -2.29 0.55 -0.55 -3.47 -1.93 -0.86 2.75 1.91 -4.76 -2.19 -2.26 2.02 1.86
Bud Neck 0.97 -3.50 -5.15 -5.48 -0.33 -0.98 -1.74 -6.99 -6.65 -0.03 -0.62 -4.02 -8.48 -8.33 0.58
Bud Site -0.88 -2.43 -2.68 -2.30 -0.38 -1.98 -1.81 -4.20 -3.71 1.46 -2.36 -3.05 -5.18 -4.35 0.60
Cell Periphery -0.77 -0.95 -1.39 -0.78 -0.87 -0.49 -2.70 1.30 1.79 2.88 -0.56 -1.80 0.00 0.62 1.89
Cytoplasm -3.73 -2.54 -4.58 -2.98 -0.15 -2.79 -1.20 -4.05 -1.05 1.16 -3.30 -1.35 -5.26 -1.79 1.07
Cytoplasmic Foci -2.40 -5.94 -5.28 -4.24 2.23 -9.71 -2.67 -8.77 -4.66 -0.12 -10.43 -7.12 -10.71 -6.31 2.59
Eisosomes -4.30 -3.44 -4.18 -0.95 -0.41 -0.86 -1.95 -2.17 -1.77 1.81 -2.54 -2.93 -3.58 -1.97 1.51
Endoplasmic Reticulum 0.56 -2.53 -1.61 -3.27 1.79 -0.98 -1.29 -1.84 -1.02 1.27 -0.58 -2.13 -3.11 -3.09 1.90
Endosome -1.50 -4.56 -5.85 -5.34 -1.09 -10.05 -1.79 -8.08 -1.95 0.84 -10.03 -6.23 -10.57 -5.11 0.54
Golgi -4.94 -4.49 -2.07 2.84 3.16 -10.67 -2.45 -6.08 1.16 0.62 -12.24 -5.98 -6.75 2.52 2.64
Lipid Particles -0.47 -2.26 -0.06 0.57 2.42 0.48 -1.00 0.17 -0.30 1.03 -0.37 -2.93 -0.15 0.23 2.90
Mitochondria 3.69 9.84 9.71 8.00 -1.57 11.84 5.96 13.15 7.56 -3.27 13.07 13.01 17.34 10.86 -3.50
Mitotic Spindle 1.02 -5.56 -5.51 -6.12 1.93 -4.70 -1.09 -6.54 -5.90 0.47 -2.57 -5.91 -8.87 -8.47 2.89
None 0.75 -1.52 0.66 -0.33 2.46 0.94 -2.29 -0.04 -0.92 2.28 0.98 -3.47 0.01 -0.96 3.43
Nuclear Periphery 1.53 -1.69 -1.10 -2.31 0.36 1.28 -1.14 -2.73 -3.97 -0.57 1.90 -3.03 -2.19 -2.94 1.64
Nuclear Periphery Foci 1.77 -1.77 -1.00 -3.31 1.16 1.02 -1.74 -3.69 -4.22 0.96 1.96 -2.77 -3.24 -4.57 1.94
Nucleolus 1.48 -0.96 -1.73 -2.51 -0.68 1.12 0.99 -1.89 -2.38 -2.33 1.58 -1.41 -2.76 -3.46 -0.41
Nucleus -0.10 -1.39 -1.86 -1.72 -0.30 0.82 0.90 -0.86 -1.60 -1.66 0.49 -1.30 -1.89 -2.30 0.24
Peroxisomes -4.12 -3.16 -5.61 -3.47 -3.41 -3.41 -0.64 -8.17 -7.04 -5.27 -5.76 -3.89 -10.03 -7.69 -5.76
Vacuole 1.29 -3.60 -4.41 -5.59 0.50 -3.67 -1.67 -5.43 -3.07 1.36 -2.77 -4.29 -7.55 -5.98 1.90
Vacuole Periphery 0.73 -3.02 -2.10 -3.70 1.98 -3.12 -1.33 -2.66 -1.26 1.16 -2.23 -3.41 -3.71 -2.57 2.17
Standard name
Human Ortholog
Description Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rtc6

Rtc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available