Standard name
Human Ortholog
Description RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0 0 0.05 0.06 0.06 0.08 0.07 0.05 0 0.08 0.14 0.08 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.94 0.95 0.86 0.8 0.69 0.68 0.66 0.67 0.68 0.65 0.97 0.99 0.96 0.95 0.91 0.95 0.93 0.92 0.85 0.87 0.88 0.91
Endoplasmic Reticulum 0 0 0.05 0 0.06 0.06 0.16 0.16 0.1 0.12 0.09 0.1 0 0 0 0 0 0 0 0 0.12 0.08 0.06 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.18 0.13 0.12 0.2 0.19 0.25 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.06 0.08 0.1 0.07 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 4 1 7 6 8 12 14 9 0 0 0 0 2 0 0 1 2 0 0 0
Bud 0 0 1 0 1 1 3 8 4 2 6 3 2 2 0 1 0 0 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 18 15 7 24 14 18 32 32 46 40 51 18 17 8 17 27 18 0 0 2 0 0 1
Cytoplasm 120 742 420 251 441 415 495 551 446 506 420 432 235 337 264 197 177 217 131 697 382 167 328 259
Endoplasmic Reticulum 1 25 23 8 29 32 113 129 69 90 54 69 4 6 6 5 7 7 3 27 52 14 20 11
Endosome 0 1 1 0 1 0 1 1 1 1 0 1 1 0 1 0 2 1 2 4 0 0 4 0
Golgi 0 2 1 1 0 0 2 2 0 1 1 2 1 0 0 0 1 0 0 2 1 0 3 0
Mitochondria 0 0 0 4 14 96 94 98 136 139 158 121 0 0 0 1 1 1 0 2 2 1 3 2
Nucleus 0 2 1 1 2 1 5 2 2 3 4 0 0 0 1 4 1 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 6 8 3 0 3 2 7 0 0 0 0 0 0 0 1 0 0 2 0
Nucleolus 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 5 5 26 22 46 63 69 54 26 66 2 0 4 4 7 6 1 6 1 4 6 4
Unique Cell Count 120 764 446 264 511 520 719 808 673 751 622 664 243 342 276 208 194 228 141 755 451 192 374 287
Labelled Cell Count 123 792 468 277 543 589 792 895 767 857 725 762 263 362 284 229 225 252 141 755 451 192 374 287


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 13.8 12.5 11.9 11.0 9.9 9.9 10.4 10.0 10.0 9.8 10.2 13.5 13.0 13.0 11.8 11.9 12.0 11.1 11.4 11.4
Std Deviation (1e-4) 1.5 1.9 2.0 2.0 1.8 1.9 1.9 1.9 2.2 2.1 2.1 2.1 1.5 1.3 1.6 2.2 2.4 2.1 1.2 1.3 1.3
Intensity Change (Log2) – – – -0.07 -0.17 -0.34 -0.33 -0.26 -0.32 -0.32 -0.35 -0.3 0.12 0.06 0.07 -0.08 -0.07 -0.06 -0.16 -0.13 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 1.0 -0.6 -0.9 0.5 1.1 2.1 2.2 3.0 2.4 1.1 -0.3 2.7 5.0 2.6
Cytoplasm 0.5 -4.0 -6.5 -10.2 -10.5 -10.9 -10.7 -10.5 -11.3 1.5 3.1 0.9 0.3 -1.4 0.5
Endoplasmic Reticulum -1.3 0.4 0.7 5.5 5.6 3.0 3.9 2.2 3.1 -2.3 -2.5 -2.0 -1.6 -0.9 -1.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.5 9.6 8.0 7.7 10.1 9.7 11.5 9.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.5 2.9 4.3 5.0 6.0 4.7 2.9 5.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.2246 10.0472 8.507 8.7002 7.0039 9.1108 10.1587 12.6368 11.5673 10.8542 10.4775 11.7355 8.3619 9.4822 8.4417 8.7841 9.3831 9.7182
Actin 0.0261 0.0003 0.0102 0.0122 0.0605 0.0108 0.0187 0.0083 0.0119 0.0207 0.0152 0.0092 0.0098 0.0006 0.036 0.0003 0.001 0.0004
Bud 0.0005 0.0001 0.0002 0.0009 0.0021 0.0003 0.0029 0.0104 0.0041 0.0331 0.0034 0.003 0.0005 0.0001 0.0014 0.0001 0.0002 0.0001
Bud Neck 0.0019 0.0001 0.0004 0.0007 0.0017 0.0011 0.0009 0.0003 0.0008 0.0014 0.0012 0.0021 0.0008 0.0003 0.0004 0.0002 0.0003 0.0005
Bud Periphery 0.0003 0 0.0002 0.0011 0.0019 0.0003 0.0024 0.004 0.0025 0.0163 0.0016 0.0022 0.0009 0.0001 0.0012 0 0 0
Bud Site 0.0025 0.0002 0.0005 0.003 0.0029 0.0004 0.0032 0.0128 0.01 0.0115 0.0011 0.0017 0.006 0.0008 0.0051 0 0.0001 0
Cell Periphery 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0005 0.0005 0.0004 0.001 0.0002 0.0002 0.0003 0.0001 0.0002 0 0 0
Cytoplasm 0.7916 0.969 0.8729 0.8273 0.702 0.911 0.7759 0.9293 0.8697 0.7731 0.8744 0.9291 0.8402 0.9682 0.8162 0.8801 0.9779 0.9344
Cytoplasmic Foci 0.0416 0.0025 0.0101 0.0066 0.0209 0.0082 0.0235 0.0042 0.0117 0.021 0.017 0.0119 0.0276 0.0034 0.0199 0.0055 0.0028 0.0058
Eisosomes 0.0002 0 0.0001 0.0001 0.0017 0.0001 0.0001 0 0.0001 0.0004 0.0001 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0402 0.0209 0.0417 0.1007 0.0198 0.023 0.0463 0.0112 0.0378 0.0511 0.0142 0.0144 0.0385 0.0065 0.0201 0.0716 0.0098 0.0287
Endosome 0.0303 0.0029 0.032 0.008 0.0738 0.0166 0.0535 0.0026 0.014 0.0148 0.0248 0.0083 0.0159 0.003 0.0309 0.0245 0.0023 0.0191
Golgi 0.0072 0.0002 0.0075 0.004 0.0145 0.0043 0.014 0.0005 0.0061 0.0022 0.0112 0.0007 0.003 0.0016 0.0207 0.0007 0.0003 0.0007
Lipid Particles 0.0078 0 0.0038 0.0029 0.0382 0.0049 0.0045 0.0001 0.0035 0.0051 0.0012 0.0003 0.0068 0.006 0.0059 0.0004 0 0
Mitochondria 0.0021 0.0001 0.0035 0.0139 0.005 0.0016 0.0144 0.0003 0.005 0.0135 0.0038 0.0008 0.0037 0.0016 0.0101 0.0003 0.0001 0.0011
None 0.0044 0.0007 0.0016 0.0009 0.0113 0.0014 0.0088 0.0026 0.0069 0.0154 0.0026 0.0032 0.0037 0.0006 0.0025 0.0007 0.001 0.0007
Nuclear Periphery 0.0145 0.0016 0.0073 0.0047 0.0115 0.0033 0.011 0.001 0.0033 0.0049 0.0015 0.005 0.0039 0.0025 0.0037 0.0103 0.0017 0.0029
Nucleolus 0.0003 0 0.0001 0.0001 0.0012 0.0001 0.0005 0.0001 0.0003 0.001 0.0181 0.0003 0.0002 0.0003 0.0003 0 0 0
Nucleus 0.0037 0.001 0.0018 0.0024 0.0034 0.0019 0.0046 0.0093 0.003 0.004 0.0036 0.003 0.0016 0.0012 0.0028 0.0027 0.0018 0.0028
Peroxisomes 0.0148 0 0.0021 0.0083 0.0127 0.0047 0.0022 0.0001 0.0016 0.0015 0.0015 0.0003 0.0152 0.0018 0.0093 0 0 0
Punctate Nuclear 0.0037 0.0001 0.0006 0.0003 0.0076 0.0032 0.004 0.0007 0.0043 0.0028 0.0008 0.0022 0.0116 0.0002 0.0112 0.0003 0.0002 0.0001
Vacuole 0.0048 0.0004 0.0027 0.0014 0.0047 0.002 0.006 0.0014 0.0022 0.0039 0.0018 0.0016 0.0075 0.0008 0.0012 0.0017 0.0004 0.0018
Vacuole Periphery 0.0014 0.0001 0.0009 0.0005 0.0023 0.0007 0.002 0.0002 0.0007 0.0013 0.0009 0.0005 0.0024 0.0002 0.0008 0.0007 0.0001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.1002 33.791 44.5767 45.6305 36.5627 43.7759 37.9623 46.6686 35.7289 46.7322
Translational Efficiency 0.6803 0.9455 0.6521 0.6847 0.7869 0.6746 0.7747 0.6077 0.7736 0.6356

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Mrn1

Mrn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available