Standard name | |
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Human Ortholog | |
Description | Conserved casein kinase; regulates diverse events including: vesicular traffic, DNA repair, the CVT pathway, monopolar attachment of sister kinetochores at meiosis I, and ribosomal subunit biogenesis; monopolin subunit; binds the RNAPII CTD; phosphorylates COPII coat subunits; interacts with Sit4p phosphatase; antagonizes calcineurin signaling, reducing nuclear accumulation of Crz1p; phosphorylates Dsn1p, the kinetochore receptor for monopolin; homolog of mammalian CK1delta |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 1 | 4 | 7 | 10 | 8 | 13 | 11 | 23 | 7 | 21 | 5 | 2 | 0 | 6 | 3 | 3 | 4 | 2 | 1 | 1 | 4 | 3 |
Bud | 0 | 0 | 0 | 0 | 8 | 8 | 18 | 10 | 15 | 13 | 20 | 19 | 2 | 3 | 0 | 3 | 1 | 1 | 10 | 7 | 3 | 5 | 19 | 30 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 4 | 2 | 7 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 9 | 6 | 9 | 16 | 0 | 2 | 5 | 3 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 104 | 12 | 44 | 31 | 71 | 126 | 191 | 156 | 113 | 153 | 120 | 145 | 100 | 151 | 161 | 105 | 86 | 98 | 78 | 25 | 52 | 34 | 76 | 136 |
Endoplasmic Reticulum | 15 | 3 | 3 | 2 | 15 | 47 | 49 | 40 | 17 | 55 | 27 | 40 | 23 | 12 | 30 | 1 | 5 | 5 | 11 | 1 | 3 | 4 | 7 | 17 |
Endosome | 1 | 0 | 3 | 5 | 15 | 9 | 46 | 30 | 23 | 19 | 10 | 9 | 2 | 1 | 6 | 3 | 9 | 3 | 15 | 3 | 4 | 1 | 15 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 2 | 2 | 11 | 5 | 14 | 15 | 25 | 12 | 23 | 27 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 4 |
Nucleus | 14 | 0 | 4 | 15 | 13 | 8 | 7 | 3 | 16 | 7 | 16 | 12 | 13 | 19 | 19 | 8 | 1 | 3 | 26 | 15 | 18 | 5 | 31 | 40 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 1 | 0 | 0 | 3 | 7 |
Nucleolus | 0 | 0 | 4 | 0 | 4 | 4 | 17 | 8 | 3 | 4 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 0 | 6 | 0 | 3 | 3 |
Vac/Vac Membrane | 27 | 1 | 11 | 42 | 61 | 12 | 68 | 50 | 55 | 40 | 36 | 19 | 67 | 68 | 70 | 15 | 39 | 19 | 14 | 6 | 4 | 8 | 37 | 51 |
Unique Cell Count | 146 | 16 | 65 | 92 | 188 | 212 | 379 | 310 | 256 | 303 | 248 | 271 | 191 | 241 | 270 | 133 | 132 | 126 | 192 | 68 | 107 | 69 | 214 | 332 |
Labelled Cell Count | 164 | 16 | 72 | 101 | 206 | 230 | 421 | 331 | 290 | 335 | 274 | 318 | 212 | 258 | 292 | 146 | 151 | 136 | 192 | 68 | 107 | 69 | 214 | 332 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 12.9 | 9.1 | 9.6 | 9.2 | 8.6 | 8.6 | 8.9 | 9.1 | 9.3 | 9.2 | 9.5 | 11.9 | 11.0 | 12.1 | 11.5 | 12.1 | 12.4 | – | 14.7 | 15.5 |
Std Deviation (1e-4) | 2.1 | 1.6 | 1.6 | 1.6 | 1.7 | 1.3 | 1.4 | 1.5 | 1.7 | 1.8 | 1.8 | 1.8 | 1.4 | 1.7 | 1.6 | 2.1 | 2.0 | 2.2 | – | 2.4 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.02 | -0.07 | -0.07 | -0.03 | 0.0 | 0.04 | 0.03 | 0.08 | 0.4 | 0.27 | 0.41 | 0.34 | 0.42 | 0.45 | – | 0.7 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.2 | -4.2 | -1.2 | -2.6 | -2.6 | -3.4 | -2.5 | -2.8 | -2.1 | -2.2 | -0.7 | -1.2 | 1.7 | -0.4 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 3.2 | 1.9 | 1.9 | 0 | 2.7 | 1.5 | 2.2 | 1.7 | 0 | 1.6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1.8 | 1.3 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 1.6 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.7 | 2.4 | -2.9 | 0.2 | -0.2 | 0.8 | -0.8 | -0.5 | -2.5 | 2.7 | 1.8 | 1.5 | -1.1 | 1.9 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.023 | 0.006 | 0.0382 | 0.0112 | 0.0096 | 0.0181 | 0.0256 | 0.0068 | 0.0683 | 0.0297 | 0.0118 | 0.0152 | 0.1744 | 0.0651 | 0.1607 | 0.1306 | 0.0004 | 0.0448 |
Bud | 0.0007 | 0.0021 | 0.0036 | 0.0013 | 0.0179 | 0.0011 | 0.0007 | 0.0011 | 0.0013 | 0.0007 | 0.003 | 0.0003 | 0.0028 | 0.0014 | 0.0011 | 0.0048 | 0.0001 | 0.0006 |
Bud Neck | 0.0217 | 0.0063 | 0.0387 | 0.0131 | 0.0138 | 0.2562 | 0.0502 | 0.0127 | 0.0344 | 0.0203 | 0.0048 | 0.2709 | 0.0385 | 0.007 | 0.0524 | 0.0108 | 0.0029 | 0.4576 |
Bud Periphery | 0.0007 | 0.0007 | 0.0013 | 0.0014 | 0.0029 | 0.0016 | 0.0005 | 0.0005 | 0.0008 | 0.0004 | 0.0045 | 0.0003 | 0.0015 | 0.0004 | 0.0003 | 0.006 | 0 | 0.0004 |
Bud Site | 0.0037 | 0.0113 | 0.0077 | 0.0027 | 0.0055 | 0.0032 | 0.0119 | 0.0162 | 0.007 | 0.0019 | 0.0012 | 0.0003 | 0.0195 | 0.0523 | 0.0064 | 0.0028 | 0 | 0.0006 |
Cell Periphery | 0.0004 | 0.0003 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0008 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0013 | 0.0006 | 0.0004 | 0.0013 | 0 | 0.0002 |
Cytoplasm | 0.6555 | 0.6373 | 0.5936 | 0.687 | 0.7129 | 0.4471 | 0.729 | 0.6982 | 0.6748 | 0.6363 | 0.7234 | 0.4934 | 0.6143 | 0.7874 | 0.6803 | 0.4558 | 0.9625 | 0.4005 |
Cytoplasmic Foci | 0.0103 | 0.0106 | 0.0128 | 0.0061 | 0.0165 | 0.0051 | 0.0108 | 0.0077 | 0.0127 | 0.03 | 0.033 | 0.0025 | 0.0517 | 0.0282 | 0.017 | 0.0129 | 0.0005 | 0.0174 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0007 | 0 | 0 |
Endoplasmic Reticulum | 0.0149 | 0.0074 | 0.0175 | 0.021 | 0.0297 | 0.0086 | 0.0161 | 0.0067 | 0.0163 | 0.0102 | 0.0326 | 0.009 | 0.0089 | 0.0032 | 0.009 | 0.0359 | 0.0007 | 0.0026 |
Endosome | 0.0082 | 0.0025 | 0.0055 | 0.004 | 0.0199 | 0.0034 | 0.0111 | 0.0063 | 0.0068 | 0.0151 | 0.0246 | 0.0019 | 0.0228 | 0.0018 | 0.002 | 0.1002 | 0.0001 | 0.0028 |
Golgi | 0.0013 | 0.0003 | 0.001 | 0.0004 | 0.0029 | 0.0007 | 0.0021 | 0.0014 | 0.0033 | 0.008 | 0.0041 | 0.0003 | 0.0159 | 0.001 | 0.0009 | 0.134 | 0 | 0.0124 |
Lipid Particles | 0.0048 | 0.0003 | 0.0012 | 0.001 | 0.0029 | 0.0006 | 0.0015 | 0.0004 | 0.0019 | 0.0025 | 0.0013 | 0.0002 | 0.002 | 0.0003 | 0.0003 | 0.001 | 0 | 0.0008 |
Mitochondria | 0.0008 | 0.0014 | 0.0007 | 0.0004 | 0.0145 | 0.0043 | 0.0013 | 0.0004 | 0.0017 | 0.001 | 0.002 | 0.0004 | 0.0021 | 0.0006 | 0.0004 | 0.0851 | 0 | 0.0074 |
None | 0.0017 | 0.0021 | 0.002 | 0.0021 | 0.004 | 0.001 | 0.0038 | 0.0013 | 0.0055 | 0.0025 | 0.0026 | 0.0011 | 0.0032 | 0.0028 | 0.002 | 0.002 | 0.0007 | 0.0005 |
Nuclear Periphery | 0.0249 | 0.0225 | 0.0275 | 0.0224 | 0.0297 | 0.0166 | 0.0248 | 0.0136 | 0.0205 | 0.0185 | 0.0245 | 0.0165 | 0.0129 | 0.0064 | 0.0149 | 0.0046 | 0.0011 | 0.0025 |
Nucleolus | 0.0005 | 0.0009 | 0.0009 | 0.0005 | 0.0008 | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.2138 | 0.2666 | 0.2154 | 0.1941 | 0.0831 | 0.2169 | 0.0935 | 0.2015 | 0.116 | 0.1644 | 0.0913 | 0.1766 | 0.0142 | 0.02 | 0.0286 | 0.0024 | 0.03 | 0.0121 |
Peroxisomes | 0.0018 | 0.0007 | 0.001 | 0.0003 | 0.002 | 0.0006 | 0.0014 | 0.0004 | 0.0023 | 0.0022 | 0.0034 | 0.0003 | 0.0033 | 0.0044 | 0.0025 | 0.0005 | 0 | 0.0293 |
Punctate Nuclear | 0.0092 | 0.0189 | 0.0286 | 0.0289 | 0.023 | 0.0123 | 0.0124 | 0.0218 | 0.0236 | 0.0548 | 0.0273 | 0.0091 | 0.009 | 0.0164 | 0.0202 | 0.0016 | 0.0009 | 0.0069 |
Vacuole | 0.0018 | 0.0016 | 0.0018 | 0.0016 | 0.0063 | 0.0014 | 0.0016 | 0.0014 | 0.0014 | 0.0008 | 0.0033 | 0.001 | 0.001 | 0.0006 | 0.0003 | 0.0031 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0016 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0038 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.7213 | 23.2479 | 34.009 | 51.0676 | 44.7969 | 27.477 | 38.2811 | 40.7955 | 41.88 | 48.8261 |
Translational Efficiency | 1.4366 | 1.6119 | 1.0627 | 0.7229 | 0.8417 | 1.5198 | 0.8499 | 0.7098 | 0.8568 | 0.8033 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
581 | 469 | 2278 | 1248 | 1703 | 833 | 1725 | 115 | 2284 | 1302 | 4003 | 1363 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 985.50 | 1263.36 | 1579.49 | 1675.04 | 1103.92 | 1150.78 | 1569.38 | 1831.29 | 1073.80 | 1191.33 | 1575.13 | 1688.22 |
Standard Deviation | 138.40 | 192.45 | 236.81 | 236.26 | 159.96 | 192.87 | 222.54 | 303.09 | 163.13 | 200.16 | 230.82 | 246.46 |
Intensity Change Log 2 | — | 0.358338 | 0.680531 | 0.765268 | — | 0.059976 | 0.507559 | 0.730225 | — | 0.208407 | 0.591732 | 0.746859 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001272 | 0.004792 | 0.011949 | 0.132636* | 0.002506 | 0.005512 | 0.040715 | 0.145151* | 0.002192 | 0.005252 | 0.024345 | 0.133692* |
Bud Neck | 0.040449 | 0.070807 | 0.215868* | 0.057273 | 0.060342 | 0.051257 | 0.134388 | 0.058276 | 0.055281 | 0.058299 | 0.180756* | 0.057358 |
Bud Site | 0.057451 | 0.127114* | 0.346574* | 0.452254* | 0.063003 | 0.089280 | 0.483960* | 0.444915* | 0.061591 | 0.102908* | 0.405778* | 0.451635* |
Cell Periphery | 0.000411 | 0.000772 | 0.001759 | 0.004945 | 0.000497 | 0.000933 | 0.002061 | 0.009172 | 0.000475 | 0.000875 | 0.001889 | 0.005301 |
Cytoplasm | 0.485633* | 0.297528* | 0.118066* | 0.139681* | 0.387443* | 0.375762* | 0.148709* | 0.096563 | 0.412421* | 0.347581* | 0.131271* | 0.136043* |
Cytoplasmic Foci | 0.064431 | 0.035059 | 0.005747 | 0.024761 | 0.046362 | 0.062519 | 0.012822 | 0.035875 | 0.050959 | 0.052628 | 0.008796 | 0.025699 |
Eisosomes | 0.000073 | 0.000086 | 0.000133 | 0.000874 | 0.000070 | 0.000136 | 0.000231 | 0.001878 | 0.000071 | 0.000118 | 0.000175 | 0.000959 |
Endoplasmic Reticulum | 0.008069 | 0.011596 | 0.005636 | 0.002575 | 0.010788 | 0.014034 | 0.004442 | 0.007128 | 0.010097 | 0.013156 | 0.005121 | 0.002960 |
Endosome | 0.006145 | 0.005286 | 0.001980 | 0.005540 | 0.004102 | 0.006859 | 0.004710 | 0.010656 | 0.004622 | 0.006292 | 0.003156 | 0.005972 |
Golgi | 0.001058 | 0.004472 | 0.000276 | 0.003259 | 0.000856 | 0.002756 | 0.001239 | 0.007306 | 0.000908 | 0.003374 | 0.000691 | 0.003600 |
Lipid Particles | 0.003860 | 0.006546 | 0.006726 | 0.043197 | 0.004917 | 0.007297 | 0.016554 | 0.074148 | 0.004648 | 0.007027 | 0.010961 | 0.045808 |
Mitochondria | 0.000763 | 0.001923 | 0.003227 | 0.001079 | 0.002088 | 0.001670 | 0.001243 | 0.000364 | 0.001751 | 0.001761 | 0.002372 | 0.001019 |
Mitotic Spindle | 0.015692 | 0.044665 | 0.050205 | 0.028455 | 0.024047 | 0.041274 | 0.035840 | 0.005832 | 0.021921 | 0.042496 | 0.044015 | 0.026546 |
None | 0.005733 | 0.005606 | 0.003832 | 0.000265 | 0.004636 | 0.003632 | 0.000741 | 0.000098 | 0.004915 | 0.004343 | 0.002500 | 0.000251 |
Nuclear Periphery | 0.000833 | 0.001297 | 0.001359 | 0.000173 | 0.002053 | 0.001133 | 0.000434 | 0.000119 | 0.001743 | 0.001192 | 0.000961 | 0.000168 |
Nuclear Periphery Foci | 0.023159 | 0.027752 | 0.002550 | 0.002612 | 0.025910 | 0.025039 | 0.004867 | 0.002440 | 0.025210 | 0.026016 | 0.003548 | 0.002598 |
Nucleolus | 0.001028 | 0.000913 | 0.000515 | 0.000072 | 0.002052 | 0.001014 | 0.000181 | 0.000022 | 0.001791 | 0.000978 | 0.000371 | 0.000067 |
Nucleus | 0.217157* | 0.291996* | 0.186998* | 0.017913 | 0.310770* | 0.228866* | 0.048411 | 0.007026 | 0.286956* | 0.251606* | 0.127277 | 0.016995 |
Peroxisomes | 0.001739 | 0.002048 | 0.001412 | 0.011378 | 0.001969 | 0.004516 | 0.004188 | 0.007014 | 0.001910 | 0.003627 | 0.002609 | 0.011009 |
Vacuole | 0.064743 | 0.059370 | 0.034942 | 0.069026 | 0.045127 | 0.075632 | 0.053959 | 0.083771 | 0.050117 | 0.069774 | 0.043137 | 0.070270 |
Vacuole Periphery | 0.000300 | 0.000370 | 0.000247 | 0.002032 | 0.000463 | 0.000881 | 0.000305 | 0.002246 | 0.000421 | 0.000697 | 0.000272 | 0.002050 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.70 | -14.30 | -19.52 | -18.57 | -17.50 | -4.45 | -13.81 | -6.06 | -5.87 | -4.04 | -6.41 | -17.45 | -20.26 | -19.54 | -16.23 |
Bud Neck | -2.77 | -20.48 | -3.18 | 0.68 | 24.30* | 2.31 | -8.77 | 1.18 | -0.51 | 7.20 | 0.74 | -20.70 | 0.80 | -0.12 | 25.30* |
Bud Site | -4.23 | -22.87* | -28.13* | -20.43 | -10.33 | -2.84 | -32.56* | -10.43 | -9.69 | -0.61 | -4.71 | -39.09 | -31.89 | -27.03 | -6.73 |
Cell Periphery | -5.82 | -16.97 | -13.26 | -11.62 | -7.84 | -4.67 | -13.35 | -5.87 | -5.50 | -4.20 | -6.50 | -20.75 | -14.13 | -12.60 | -8.38 |
Cytoplasm | 10.38 | 24.20 | 19.67 | 8.06 | -5.17 | 1.26 | 21.35 | 12.49 | 11.11 | 3.53 | 6.65 | 34.48 | 25.36 | 15.99 | -1.94 |
Cytoplasmic Foci | 5.63 | 13.13 | 7.33 | 1.18 | -11.83 | -2.15 | 14.74 | 0.30 | 1.19 | -3.61 | 1.26 | 21.25 | 8.42 | 6.23 | -10.65 |
Eisosomes | -1.25 | -6.68 | -11.46 | -11.17 | -10.62 | -4.91 | -14.01 | -3.85 | -3.60 | -3.25 | -5.06 | -15.62 | -12.14 | -11.20 | -10.41 |
Endoplasmic Reticulum | -3.70 | 0.23 | 7.24 | 9.49 | 13.85 | -2.50 | 7.71 | 3.98 | 5.02 | 1.36 | -3.75 | 6.41 | 15.68 | 14.80 | 13.58 |
Endosome | 2.53 | 7.34 | 4.02 | 1.96 | -3.31 | -2.86 | 2.09 | -0.43 | 0.39 | -0.87 | -1.36 | 8.17 | 2.69 | 3.37 | -2.10 |
Golgi | -1.12 | 2.84 | -1.46 | 0.51 | -3.07 | -1.56 | 0.55 | -1.59 | -0.87 | -1.66 | -1.89 | 2.86 | -2.12 | 0.26 | -3.04 |
Lipid Particles | -3.63 | -7.40 | -12.95 | -11.32 | -10.85 | -3.05 | -10.16 | -5.02 | -4.82 | -3.71 | -4.22 | -11.60 | -13.47 | -12.46 | -10.08 |
Mitochondria | -2.19 | -17.05 | -0.07 | 2.17 | 17.57* | 1.18 | 0.87 | 4.18 | 4.33 | 8.63 | 0.31 | -5.76 | 2.97 | 3.30 | 15.83 |
Mitotic Spindle | -3.68 | -7.96 | -1.37 | 2.96 | 7.67 | -2.05 | -3.50 | 1.12 | 2.28 | 2.91 | -3.69 | -8.34 | 0.13 | 3.52 | 7.43 |
None | -1.28 | 1.96 | 7.55 | 8.56 | 9.09 | 1.89 | 13.54 | 17.25 | 7.39 | 9.78 | 0.36 | 6.81 | 17.91 | 10.96 | 10.53 |
Nuclear Periphery | -5.03 | -14.11 | 9.07 | 9.56 | 22.40 | 5.26 | 6.94 | 13.11 | 12.27 | 14.51 | 2.58 | -2.06 | 14.00 | 14.74 | 25.07 |
Nuclear Periphery Foci | -1.58 | 7.76 | 8.36 | 8.16 | 3.05 | 0.68 | 12.32 | 13.14 | 9.11 | 2.55 | -0.34 | 14.99 | 16.54 | 12.66 | 5.25 |
Nucleolus | -1.05 | 3.01 | 4.05 | 3.57 | 7.28 | 2.87 | 10.39 | 11.14 | 4.34 | 10.74 | 0.85 | 9.31 | 11.13 | 5.27 | 8.44 |
Nucleus | -5.10 | -4.86 | 18.44 | 21.07 | 37.15 | 6.63 | 25.47 | 39.46 | 23.88 | 17.22 | 2.74 | 11.34 | 42.48 | 30.44 | 36.97 |
Peroxisomes | 0.50 | 4.61 | -6.36 | -6.44 | -7.60 | -2.83 | -1.30 | -1.90 | -0.99 | -1.65 | -2.49 | 2.15 | -6.51 | -4.96 | -7.05 |
Vacuole | -0.30 | 1.31 | -10.45 | -9.71 | -14.62 | -8.77 | -16.93 | -6.46 | -4.34 | -2.71 | -7.70 | -12.68 | -18.32 | -11.64 | -11.52 |
Vacuole Periphery | -0.51 | 0.26 | -1.61 | -1.54 | -1.64 | -0.83 | 1.45 | -0.66 | -0.42 | -0.82 | -0.80 | 1.41 | -1.51 | -1.13 | -1.79 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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