Standard name | |
---|---|
Human Ortholog | |
Description | Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 15 | 37 | 47 | 10 | 25 | 18 | 34 | 27 | 14 | – | 10 | 10 | 11 | 17 | 7 | 16 | 14 | 19 | 4 | 8 | 12 | 3 | 14 | 16 |
Bud | 5 | 7 | 9 | 1 | 8 | 7 | 18 | 9 | 15 | – | 17 | 9 | 3 | 5 | 3 | 3 | 6 | 6 | 0 | 1 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 8 | 2 | 3 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 9 | 39 | 80 | 13 | 60 | 20 | 46 | 29 | 22 | – | 21 | 12 | 26 | 100 | 64 | 78 | 69 | 67 | 8 | 11 | 43 | 16 | 71 | 77 |
Cytoplasm | 22 | 43 | 54 | 16 | 86 | 95 | 213 | 149 | 158 | – | 121 | 103 | 22 | 103 | 117 | 46 | 34 | 26 | 1 | 11 | 22 | 2 | 3 | 11 |
Endoplasmic Reticulum | 26 | 60 | 45 | 10 | 54 | 34 | 76 | 48 | 26 | – | 13 | 17 | 22 | 66 | 46 | 1 | 1 | 3 | 83 | 116 | 135 | 66 | 121 | 130 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 0 | 0 | 2 | 1 |
Mitochondria | 0 | 1 | 0 | 1 | 0 | 12 | 17 | 10 | 24 | – | 32 | 12 | 1 | 1 | 1 | 2 | 1 | 2 | 0 | 3 | 25 | 1 | 6 | 4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 4 | 2 | – | 3 | 1 | 0 | 3 | 0 | 1 | 1 | 1 | 1 | 4 | 17 | 2 | 4 | 7 |
Unique Cell Count | 68 | 137 | 194 | 41 | 190 | 158 | 342 | 231 | 225 | 185 | 144 | 60 | 218 | 185 | 124 | 111 | 102 | 109 | 168 | 269 | 101 | 231 | 264 | |
Labelled Cell Count | 78 | 187 | 236 | 51 | 235 | 192 | 410 | 281 | 262 | 220 | 166 | 85 | 298 | 240 | 149 | 129 | 128 | 109 | 168 | 269 | 101 | 231 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.4 | 10.2 | 8.5 | 8.1 | 8.7 | 6.2 | 6.7 | 6.7 | 5.8 | – | 5.3 | 6.0 | 9.0 | 8.6 | 7.9 | 16.7 | 17.0 | 16.5 | 9.4 | 8.8 | 8.6 |
Std Deviation (1e-4) | 1.5 | 1.5 | 0.9 | 0.9 | 1.1 | 0.9 | 1.0 | 1.0 | 0.8 | – | 0.7 | 1.0 | 1.3 | 1.1 | 1.4 | 3.2 | 2.8 | 3.1 | 1.2 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.04 | -0.45 | -0.33 | -0.34 | -0.54 | – | -0.67 | -0.51 | 0.09 | 0.02 | -0.11 | 0.97 | 1.01 | 0.96 | 0.15 | 0.05 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.8 | -3.1 | -4.4 | -3.4 | -5.2 | – | -5.1 | -4.2 | -1.0 | -4.6 | -5.7 | -2.5 | -2.4 | -1.1 |
Bud | 0 | -0.2 | -0.1 | 0.3 | -0.4 | 0.9 | – | 1.8 | 0.7 | 0 | -1.3 | -1.7 | 0 | 0.3 | 0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.1 | -2.0 | -5.9 | -7.3 | -6.7 | -7.5 | – | -6.6 | -6.7 | 0.3 | 0.9 | -1.3 | 3.8 | 3.5 | 4.0 |
Cytoplasm | 1.4 | 3.5 | 6.1 | 7.7 | 7.5 | 8.7 | – | 7.3 | 8.0 | 1.3 | 4.0 | 6.9 | 1.7 | 0.5 | -0.4 |
Endoplasmic Reticulum | 0.2 | 1.2 | -0.4 | -0.3 | -0.6 | -3.2 | – | -4.4 | -2.7 | 2.1 | 1.6 | 0.4 | -5.5 | -5.2 | -4.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.9 | 3.2 | 0 | 4.7 | – | 6.1 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.8583 | 10.6085 | 9.5274 | 8.8093 | 9.0063 | 9.8757 | 8.4472 | 9.1668 | 8.7358 | 7.46 | 8.138 | 8.6846 | 6.9336 | 7.0946 | 6.7123 | 7.0325 | 6.2607 | 7.0412 |
Actin | 0.07 | 0.0051 | 0.0287 | 0.0078 | 0.0202 | 0.019 | 0.012 | 0.0062 | 0.0123 | 0.0391 | 0.0173 | 0.0119 | 0.0187 | 0.0034 | 0.0114 | 0.0025 | 0.0229 | 0.0102 |
Bud | 0.0013 | 0.0004 | 0.0025 | 0.0005 | 0.0004 | 0.0005 | 0.0013 | 0.0005 | 0.0011 | 0.0005 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0.0002 | 0.0003 | 0.0003 |
Bud Neck | 0.0024 | 0.0037 | 0.0073 | 0.0202 | 0.0148 | 0.023 | 0.0016 | 0.0025 | 0.003 | 0.0251 | 0.0102 | 0.0144 | 0.0021 | 0.0042 | 0.0051 | 0.0031 | 0.0111 | 0.0207 |
Bud Periphery | 0.0099 | 0.002 | 0.0186 | 0.0079 | 0.0032 | 0.0047 | 0.0087 | 0.004 | 0.0085 | 0.0033 | 0.006 | 0.002 | 0.0024 | 0.0011 | 0.0066 | 0.004 | 0.0031 | 0.0031 |
Bud Site | 0.0068 | 0.0375 | 0.0128 | 0.0038 | 0.0026 | 0.0017 | 0.0017 | 0.013 | 0.0093 | 0.0009 | 0.0013 | 0.0002 | 0.0026 | 0.0162 | 0.0116 | 0.0003 | 0.0003 | 0.0008 |
Cell Periphery | 0.0913 | 0.1463 | 0.1653 | 0.1195 | 0.1272 | 0.0738 | 0.0775 | 0.162 | 0.0998 | 0.0519 | 0.0992 | 0.059 | 0.0764 | 0.1066 | 0.1068 | 0.0781 | 0.0659 | 0.0809 |
Cytoplasm | 0.1316 | 0.189 | 0.1497 | 0.0649 | 0.1446 | 0.1512 | 0.1661 | 0.2744 | 0.1217 | 0.141 | 0.1523 | 0.0946 | 0.1592 | 0.1819 | 0.1851 | 0.0871 | 0.1373 | 0.1774 |
Cytoplasmic Foci | 0.0157 | 0.0028 | 0.0072 | 0.017 | 0.0166 | 0.0092 | 0.01 | 0.0032 | 0.0058 | 0.0101 | 0.0092 | 0.0035 | 0.0085 | 0.0022 | 0.0057 | 0.0087 | 0.0072 | 0.004 |
Eisosomes | 0.0031 | 0.0012 | 0.0016 | 0.0012 | 0.0017 | 0.0015 | 0.0013 | 0.0015 | 0.0019 | 0.005 | 0.0019 | 0.0009 | 0.002 | 0.0009 | 0.0013 | 0.0004 | 0.008 | 0.0014 |
Endoplasmic Reticulum | 0.5145 | 0.5462 | 0.5164 | 0.6257 | 0.5717 | 0.6478 | 0.6082 | 0.4858 | 0.6613 | 0.6189 | 0.5842 | 0.7653 | 0.6438 | 0.6297 | 0.5976 | 0.7359 | 0.6317 | 0.6555 |
Endosome | 0.0228 | 0.0021 | 0.0109 | 0.0294 | 0.0132 | 0.0086 | 0.0199 | 0.0034 | 0.0078 | 0.0314 | 0.0233 | 0.0061 | 0.0133 | 0.0025 | 0.0108 | 0.0057 | 0.0123 | 0.0086 |
Golgi | 0.0209 | 0.0033 | 0.0122 | 0.0148 | 0.0288 | 0.0142 | 0.021 | 0.0121 | 0.0182 | 0.0176 | 0.0324 | 0.0123 | 0.0086 | 0.0039 | 0.0093 | 0.0067 | 0.0205 | 0.009 |
Lipid Particles | 0.055 | 0.0322 | 0.0309 | 0.0573 | 0.0247 | 0.0185 | 0.0299 | 0.0191 | 0.0151 | 0.0108 | 0.0083 | 0.0162 | 0.0156 | 0.0283 | 0.0149 | 0.0296 | 0.0182 | 0.0137 |
Mitochondria | 0.0153 | 0.0002 | 0.0023 | 0.0011 | 0.0059 | 0.004 | 0.0045 | 0.0004 | 0.0111 | 0.0014 | 0.0402 | 0.0035 | 0.0047 | 0.0002 | 0.0057 | 0.002 | 0.0503 | 0.0028 |
None | 0.018 | 0.002 | 0.0064 | 0.0008 | 0.0014 | 0.0009 | 0.0062 | 0.0006 | 0.0074 | 0.0143 | 0.0007 | 0.0008 | 0.0051 | 0.001 | 0.0041 | 0.0006 | 0.0022 | 0.0012 |
Nuclear Periphery | 0.0057 | 0.0059 | 0.0057 | 0.0074 | 0.004 | 0.0033 | 0.0082 | 0.0032 | 0.0051 | 0.0057 | 0.0031 | 0.0025 | 0.0145 | 0.0068 | 0.009 | 0.0039 | 0.0033 | 0.0034 |
Nucleolus | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0008 | 0.0008 | 0.001 | 0.0008 | 0.0005 | 0.0008 | 0.0016 | 0.0005 | 0.0007 | 0.002 | 0.0007 | 0.0004 | 0.0028 | 0.0022 | 0.0012 | 0.0005 | 0.0005 | 0.0007 |
Peroxisomes | 0.0024 | 0.0001 | 0.0016 | 0.0042 | 0.0065 | 0.0045 | 0.0063 | 0 | 0.0016 | 0.0009 | 0.0002 | 0.0001 | 0.0045 | 0 | 0.002 | 0.0231 | 0.0015 | 0.0001 |
Punctate Nuclear | 0.001 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0025 | 0.0001 | 0.0003 | 0.0077 | 0.0002 | 0.0001 | 0.0018 | 0.0003 | 0.0019 | 0.0002 | 0.0003 | 0.0002 |
Vacuole | 0.0104 | 0.0186 | 0.0175 | 0.0138 | 0.011 | 0.0117 | 0.0095 | 0.0071 | 0.007 | 0.0109 | 0.0072 | 0.0054 | 0.0093 | 0.008 | 0.0078 | 0.0071 | 0.0027 | 0.0054 |
Vacuole Periphery | 0.0008 | 0.0002 | 0.0008 | 0.0009 | 0.0006 | 0.0006 | 0.0013 | 0.0002 | 0.0007 | 0.0008 | 0.0011 | 0.0004 | 0.0031 | 0.0002 | 0.001 | 0.0003 | 0.0003 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.1067 | 35.2712 | 38.507 | 34.4494 | 39.6902 | 29.4622 | 32.6907 | 35.2748 | 31.2663 | 37.3964 |
Translational Efficiency | 1.2031 | 0.9919 | 0.7361 | 0.7829 | 0.7184 | 0.8117 | 0.836 | 0.5002 | 0.5254 | 0.5979 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2292 | 371 | 1089 | 1659 | 1620 | 1655 | 87 | 2570 | 3912 | 2026 | 1176 | 4229 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 889.98 | 1157.96 | 1279.20 | 1520.80 | 986.47 | 1054.86 | 1434.33 | 1511.89 | 929.94 | 1073.74 | 1290.68 | 1515.39 |
Standard Deviation | 108.52 | 165.01 | 169.68 | 189.73 | 134.44 | 165.65 | 182.55 | 208.29 | 129.01 | 170.27 | 175.43 | 201.26 |
Intensity Change Log 2 | — | 0.379741 | 0.523397 | 0.772986 | — | 0.096704 | 0.540030 | 0.616006 | — | 0.237880 | 0.532165 | 0.692592 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005203 | 0.010459 | 0.020877 | 0.013381 | 0.006026 | 0.009536 | 0.023287 | 0.006848 | 0.005544 | 0.009705 | 0.021055 | 0.009411 |
Bud Neck | 0.020875 | 0.015181 | 0.019462 | 0.007904 | 0.010806 | 0.010124 | 0.018506 | 0.005868 | 0.016705 | 0.011050 | 0.019391 | 0.006667 |
Bud Site | 0.043606 | 0.066708 | 0.069958 | 0.050737 | 0.036378 | 0.045890 | 0.053767 | 0.031150 | 0.040613 | 0.049702 | 0.068761 | 0.038834 |
Cell Periphery | 0.050879 | 0.175447* | 0.022932 | 0.157278* | 0.063618 | 0.137005* | 0.117072* | 0.212101* | 0.056154 | 0.144044* | 0.029896 | 0.190594* |
Cytoplasm | 0.350698* | 0.151165* | 0.193614* | 0.081862 | 0.304779* | 0.211574* | 0.048593 | 0.110664* | 0.331682* | 0.200512* | 0.182885* | 0.099365 |
Cytoplasmic Foci | 0.045714 | 0.030005 | 0.022737 | 0.008647 | 0.034315 | 0.030799 | 0.007495 | 0.006895 | 0.040993 | 0.030653 | 0.021609 | 0.007582 |
Eisosomes | 0.009719 | 0.006120 | 0.001893 | 0.002282 | 0.008467 | 0.007288 | 0.004271 | 0.002387 | 0.009200 | 0.007074 | 0.002069 | 0.002346 |
Endoplasmic Reticulum | 0.283158* | 0.421529* | 0.416496* | 0.476676* | 0.407488* | 0.399355* | 0.611973* | 0.428180* | 0.334644* | 0.403416* | 0.430957* | 0.447204* |
Endosome | 0.009247 | 0.012640 | 0.016029 | 0.008657 | 0.006950 | 0.008333 | 0.009746 | 0.005363 | 0.008296 | 0.009122 | 0.015564 | 0.006656 |
Golgi | 0.022343 | 0.014016 | 0.004131 | 0.007174 | 0.016510 | 0.013725 | 0.003829 | 0.004745 | 0.019927 | 0.013778 | 0.004108 | 0.005698 |
Lipid Particles | 0.035997 | 0.036738 | 0.041490 | 0.129380* | 0.029517 | 0.041083 | 0.034715 | 0.106460* | 0.033314 | 0.040287 | 0.040989 | 0.115451* |
Mitochondria | 0.030537 | 0.003369 | 0.000633 | 0.002499 | 0.011057 | 0.009617 | 0.000255 | 0.004716 | 0.022470 | 0.008473 | 0.000605 | 0.003846 |
Mitotic Spindle | 0.000249 | 0.000460 | 0.003546 | 0.002018 | 0.000115 | 0.000363 | 0.000354 | 0.000826 | 0.000193 | 0.000381 | 0.003310 | 0.001294 |
None | 0.002174 | 0.000048 | 0.001460 | 0.000553 | 0.001430 | 0.000713 | 0.000049 | 0.001351 | 0.001866 | 0.000591 | 0.001356 | 0.001038 |
Nuclear Periphery | 0.000213 | 0.000310 | 0.001411 | 0.001531 | 0.000198 | 0.000344 | 0.000445 | 0.002660 | 0.000207 | 0.000338 | 0.001339 | 0.002217 |
Nuclear Periphery Foci | 0.000554 | 0.001255 | 0.027699 | 0.003696 | 0.000852 | 0.000898 | 0.010212 | 0.002244 | 0.000677 | 0.000963 | 0.026406 | 0.002814 |
Nucleolus | 0.000196 | 0.000052 | 0.000079 | 0.000066 | 0.000092 | 0.000113 | 0.000014 | 0.000124 | 0.000153 | 0.000102 | 0.000074 | 0.000101 |
Nucleus | 0.006121 | 0.001889 | 0.034822 | 0.003059 | 0.004020 | 0.002033 | 0.003667 | 0.002928 | 0.005251 | 0.002007 | 0.032517 | 0.002979 |
Peroxisomes | 0.066224 | 0.024993 | 0.000753 | 0.010518 | 0.045101 | 0.060725 | 0.001206 | 0.032666 | 0.057477 | 0.054182 | 0.000786 | 0.023978 |
Vacuole | 0.012173 | 0.023606 | 0.099402 | 0.029638 | 0.009948 | 0.008027 | 0.050238 | 0.029191 | 0.011252 | 0.010880 | 0.095765 | 0.029367 |
Vacuole Periphery | 0.004122 | 0.004009 | 0.000578 | 0.002446 | 0.002333 | 0.002455 | 0.000304 | 0.002633 | 0.003381 | 0.002740 | 0.000558 | 0.002559 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.09 | -9.08 | -6.53 | -1.53 | 3.58 | -3.27 | -2.41 | -1.26 | 2.32 | 2.28 | -4.72 | -9.25 | -6.08 | 0.11 | 6.61 |
Bud Neck | 2.17 | -0.15 | 8.88 | 2.94 | 9.98 | 0.59 | -1.76 | 5.12 | 4.71 | 2.86 | 5.01 | -3.09 | 10.88 | 5.02 | 12.20 |
Bud Site | -3.21 | -6.10 | -2.17 | 2.00 | 3.96 | -2.67 | -1.68 | 1.63 | 4.23 | 2.12 | -2.99 | -7.19 | 0.48 | 3.30 | 7.39 |
Cell Periphery | -10.23 | 11.22 | -19.30 | 1.50 | -25.58* | -12.94 | -2.60 | -27.54* | -11.51 | -4.85 | -18.26 | 9.91 | -35.27 | -8.18 | -40.62* |
Cytoplasm | 18.61 | 19.13 | 43.46 | 6.65 | 14.85 | 11.53 | 21.26 | 27.75 | 16.12 | -5.58 | 21.64 | 20.30 | 50.34 | 18.94 | 12.54 |
Cytoplasmic Foci | 3.86 | 10.86 | 22.01 | 5.48 | 8.94 | 1.51 | 7.05 | 15.46 | 14.06 | 0.46 | 5.60 | 10.69 | 27.54 | 14.88 | 10.24 |
Eisosomes | 3.95 | 17.88 | 15.92 | 4.59 | -1.80 | 1.41 | 3.83 | 8.28 | 11.35 | 2.21 | 3.96 | 17.35 | 17.07 | 12.23 | -1.90 |
Endoplasmic Reticulum | -7.30 | -9.08 | -18.03 | -2.59 | -6.32 | 0.73 | -4.89 | -1.89 | -2.70 | 4.42 | -7.81 | -6.36 | -15.33 | -4.65 | -3.62 |
Endosome | -1.91 | -7.56 | 0.65 | 2.13 | 7.52 | -2.26 | -1.26 | 3.68 | 5.37 | 1.95 | -1.38 | -9.08 | 3.85 | 4.15 | 11.18 |
Golgi | 4.22 | 18.30 | 11.20 | 3.46 | -3.26 | 2.23 | 13.02 | 11.10 | 8.04 | -1.18 | 5.88 | 22.10 | 16.60 | 8.31 | -2.96 |
Lipid Particles | -0.18 | -2.85 | -18.74 | -14.62 | -16.12 | -5.22 | -1.02 | -21.24 | -16.79 | -9.48 | -3.71 | -4.08 | -27.93 | -22.82 | -20.56 |
Mitochondria | 19.98* | 23.36 | 21.16 | 1.48 | -5.27 | 1.21 | 11.48 | 6.46 | 6.25 | -14.14 | 13.37 | 25.45 | 21.01 | 7.19 | -13.91 |
Mitotic Spindle | -0.82 | -3.82 | -3.66 | -2.97 | 1.86 | -2.26 | -3.34 | -7.67 | -3.18 | -3.92 | -1.77 | -3.88 | -5.43 | -4.13 | 2.66 |
None | 7.77 | 1.11 | 5.58 | -5.35 | 1.25 | 1.76 | 4.04 | 0.18 | -1.74 | -4.44 | 4.59 | 0.90 | 2.95 | -1.75 | 0.39 |
Nuclear Periphery | -2.28 | -7.96 | -13.22 | -11.28 | 0.19 | -8.16 | -3.49 | -19.29 | -18.07 | -14.87 | -8.54 | -8.10 | -23.05 | -21.29 | -4.06 |
Nuclear Periphery Foci | -5.45 | -17.90 | -8.58 | -6.40 | 15.41 | -0.59 | -3.54 | -5.62 | -5.56 | 3.04 | -5.45 | -18.09 | -10.32 | -8.92 | 16.43 |
Nucleolus | 13.89* | 11.15 | 13.16 | -1.81 | 2.27 | -0.72 | 12.61 | -4.44 | -0.07 | -18.08* | 1.37 | 9.85 | 7.91 | 0.21 | -3.49 |
Nucleus | 9.95 | -15.89 | 6.96 | -3.12 | 17.12 | 7.45 | -0.16 | 3.48 | -4.58 | 1.19 | 15.29 | -16.14 | 8.96 | -5.59 | 17.22 |
Peroxisomes | 12.65 | 28.29* | 22.66 | 5.95 | -11.46 | -4.71 | 19.83 | 4.80 | 9.82 | -21.41* | 1.24 | 34.56* | 17.77 | 13.17 | -24.41* |
Vacuole | -3.33 | -23.18 | -11.77 | -1.89 | 18.22 | 1.96 | -4.01 | -14.73 | -18.01 | 2.23 | 0.30 | -23.70 | -18.58 | -16.56 | 18.90 |
Vacuole Periphery | 0.11 | 12.80 | 3.01 | 1.46 | -3.78 | -0.45 | 10.47 | -0.76 | -0.46 | -5.94 | 2.15 | 15.04 | 2.30 | 0.43 | -6.47 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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