Standard name | |
---|---|
Human Ortholog | |
Description | Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 4 | 2 | 10 | 4 | 6 | 7 | 5 | – | 16 | 6 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 4 | 10 | 4 | 14 | 15 | 12 | 13 | 6 | – | 11 | 7 | 0 | 0 | 0 | 9 | 10 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 394 | 326 | 456 | 236 | 349 | 480 | 450 | 363 | 334 | – | 323 | 259 | 138 | 155 | 144 | 89 | 68 | 41 | 382 | 316 | 460 | 40 | 111 | 138 |
Endoplasmic Reticulum | 0 | 1 | 0 | 2 | 17 | 14 | 51 | 63 | 31 | – | 77 | 40 | 0 | 0 | 0 | 3 | 4 | 3 | 0 | 1 | 2 | 0 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | – | 5 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | – | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 6 | 2 |
Unique Cell Count | 394 | 327 | 458 | 241 | 375 | 504 | 510 | 444 | 377 | 422 | 313 | 139 | 157 | 144 | 94 | 72 | 41 | 389 | 338 | 475 | 44 | 125 | 163 | |
Labelled Cell Count | 398 | 332 | 472 | 246 | 390 | 513 | 519 | 451 | 381 | 435 | 320 | 139 | 157 | 144 | 102 | 83 | 45 | 389 | 338 | 475 | 44 | 125 | 163 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 144.5 | 133.0 | 135.2 | 108.9 | 144.7 | 146.5 | 151.9 | 152.7 | 143.2 | – | 132.7 | 145.7 | 133.8 | 182.5 | 203.9 | 404.3 | 415.6 | 361.0 | – | 171.1 | 252.7 |
Std Deviation (1e-4) | 48.9 | 53.6 | 50.8 | 51.0 | 48.5 | 41.8 | 46.1 | 49.9 | 50.3 | – | 46.2 | 53.3 | 44.8 | 58.4 | 64.5 | 159.3 | 135.0 | 165.8 | – | 57.1 | 108.9 |
Intensity Change (Log2) | – | – | – | -0.31 | 0.1 | 0.12 | 0.17 | 0.18 | 0.08 | – | -0.03 | 0.11 | -0.01 | 0.43 | 0.59 | 1.58 | 1.62 | 1.42 | – | 0.34 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2.0 | 0 | 0 | 1.0 | 0 | – | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.3 | 0.8 | 0.2 | 0.7 | -0.6 | – | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.1 | -5.2 | -4.1 | -7.2 | -9.2 | -7.0 | – | -10.7 | -8.8 | -0.4 | -1.1 | 0.8 | -3.9 | -3.8 | 0.4 |
Endoplasmic Reticulum | 0 | 4.6 | 3.6 | 7.0 | 8.4 | 6.3 | – | 9.6 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 56.1238 | 88.976 | 72.9336 | 61.6881 | 72.6916 | 69.1789 | 77.0377 | 111.1848 | 101.1799 | 86.8495 | 76.2118 | 107.871 | 71.6126 | 103.5199 | 86.5988 | 88.3481 | 75.4265 | 91.4043 |
Actin | 0.0122 | 0.0003 | 0.0001 | 0.0001 | 0.0023 | 0.0085 | 0.0043 | 0.0002 | 0.0009 | 0.001 | 0.0053 | 0.0001 | 0.0055 | 0.0001 | 0.0004 | 0.0001 | 0.0045 | 0.0092 |
Bud | 0.0019 | 0.0031 | 0.0005 | 0.0002 | 0.0006 | 0.0005 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0028 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0009 | 0.0001 |
Bud Neck | 0.0008 | 0.0001 | 0.0003 | 0.0003 | 0.0008 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0003 |
Bud Periphery | 0.0017 | 0.0005 | 0.0003 | 0.0001 | 0.002 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0035 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0006 | 0.0001 |
Bud Site | 0.0021 | 0.0002 | 0.0003 | 0.0001 | 0.0018 | 0.0003 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0025 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0009 | 0 |
Cell Periphery | 0.0014 | 0.0001 | 0.0002 | 0.0004 | 0.003 | 0.0001 | 0.001 | 0.0001 | 0.0012 | 0.0002 | 0.0029 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0 |
Cytoplasm | 0.845 | 0.9912 | 0.9779 | 0.9375 | 0.9197 | 0.9157 | 0.94 | 0.9918 | 0.9826 | 0.925 | 0.8404 | 0.9826 | 0.9312 | 0.9896 | 0.961 | 0.9674 | 0.8602 | 0.9777 |
Cytoplasmic Foci | 0.0176 | 0.0004 | 0.0046 | 0.0093 | 0.0128 | 0.0112 | 0.009 | 0.0022 | 0.0003 | 0.0162 | 0.0112 | 0.0036 | 0.009 | 0.0003 | 0.0076 | 0.0076 | 0.0219 | 0.0005 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.003 | 0.0002 | 0.0003 | 0.0017 | 0.0089 | 0.0017 | 0.0015 | 0.0002 | 0.0005 | 0.0005 | 0.0038 | 0.0005 | 0.0019 | 0.0002 | 0.0006 | 0.0004 | 0.0012 | 0.0006 |
Endosome | 0.0175 | 0 | 0.0003 | 0.0024 | 0.0033 | 0.0082 | 0.005 | 0.0001 | 0.0002 | 0.0024 | 0.0064 | 0.0004 | 0.004 | 0 | 0.001 | 0.0009 | 0.012 | 0.0009 |
Golgi | 0.0057 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0021 | 0.0017 | 0.0001 | 0.0003 | 0.0006 | 0.0013 | 0 | 0.0014 | 0 | 0.0004 | 0.0001 | 0.0015 | 0.0008 |
Lipid Particles | 0.0095 | 0 | 0.0009 | 0.0012 | 0.0059 | 0.0027 | 0.0035 | 0.0014 | 0.0015 | 0.0064 | 0.0052 | 0.0006 | 0.0058 | 0 | 0.0085 | 0.0015 | 0.0059 | 0.0001 |
Mitochondria | 0.0081 | 0.0003 | 0.0018 | 0.0013 | 0.0021 | 0.0058 | 0.0064 | 0.0007 | 0.0062 | 0.0021 | 0.0371 | 0.0003 | 0.0043 | 0.0006 | 0.0032 | 0.0027 | 0.0328 | 0.0002 |
None | 0.0124 | 0.0003 | 0.0012 | 0.0162 | 0.0091 | 0.004 | 0.0041 | 0.0003 | 0.0004 | 0.0128 | 0.0186 | 0.0008 | 0.005 | 0.0002 | 0.0037 | 0.006 | 0.0186 | 0.0004 |
Nuclear Periphery | 0.0092 | 0.0005 | 0.0007 | 0.0043 | 0.0056 | 0.0109 | 0.0029 | 0.0005 | 0.0007 | 0.0028 | 0.0079 | 0.0009 | 0.0054 | 0.0012 | 0.001 | 0.0007 | 0.0024 | 0.004 |
Nucleolus | 0.0071 | 0 | 0.004 | 0.0023 | 0.0024 | 0.0009 | 0.0017 | 0.0002 | 0.0001 | 0.0073 | 0.011 | 0.0022 | 0.0042 | 0.0042 | 0.0015 | 0.0029 | 0.0049 | 0 |
Nucleus | 0.0165 | 0.0015 | 0.0023 | 0.004 | 0.0051 | 0.0071 | 0.0052 | 0.0011 | 0.0014 | 0.0039 | 0.0087 | 0.0035 | 0.008 | 0.0027 | 0.0018 | 0.0027 | 0.0065 | 0.0037 |
Peroxisomes | 0.0054 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0011 | 0.0008 | 0.0002 | 0.0001 | 0.0071 | 0.0018 | 0.0001 | 0.0043 | 0 | 0.0041 | 0.0004 | 0.003 | 0 |
Punctate Nuclear | 0.0021 | 0 | 0.0004 | 0.0014 | 0.0019 | 0.0027 | 0.0012 | 0.0001 | 0 | 0.0078 | 0.0007 | 0.0005 | 0.0015 | 0.0001 | 0.0003 | 0.0006 | 0.0038 | 0.0006 |
Vacuole | 0.0151 | 0.0007 | 0.0024 | 0.0119 | 0.0093 | 0.0104 | 0.0072 | 0.0003 | 0.0016 | 0.0015 | 0.0171 | 0.002 | 0.0044 | 0.0002 | 0.0023 | 0.0032 | 0.0127 | 0.0003 |
Vacuole Periphery | 0.0055 | 0.0002 | 0.001 | 0.0049 | 0.0022 | 0.0047 | 0.0019 | 0.0001 | 0.0009 | 0.0016 | 0.0099 | 0.0006 | 0.0018 | 0.0001 | 0.0009 | 0.0015 | 0.0039 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 372.9818 | 602.4767 | 633.6553 | 502.567 | 529.2593 | 507.9063 | 615.0164 | 675.0226 | 833.4645 | 461.4782 |
Translational Efficiency | 1.4463 | 1.6276 | 1.2102 | 2.1699 | 1.5838 | 1.0934 | 1.4781 | 1.7678 | 1.3955 | 1.5657 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
857 | 1382 | 1869 | 120 | 1779 | 1477 | 2132 | 15 | 2636 | 2859 | 4001 | 135 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 11417.51 | 8605.48 | 11703.10 | 8364.51 | 6296.77 | 7715.97 | 17158.80 | 10487.21 | 7961.59 | 8145.95 | 14610.26 | 8600.37 |
Standard Deviation | 3715.48 | 3389.85 | 5609.34 | 2529.01 | 1891.83 | 3244.98 | 5606.72 | 2101.04 | 3557.68 | 3345.46 | 6233.62 | 2573.08 |
Intensity Change Log 2 | — | -0.407920 | 0.035643 | -0.448895 | — | 0.293236 | 1.446265 | 0.735947 | — | -0.118144 | 0.704253 | 0.089783 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000082 | 0.000078 | 0.000071 | 0.000109 | 0.000043 | 0.000076 | 0.000057 | 0.000613 | 0.000055 | 0.000077 | 0.000063 | 0.000165 |
Bud Neck | 0.000887 | 0.000371 | 0.002237 | 0.003110 | 0.000430 | 0.000433 | 0.000837 | 0.003641 | 0.000578 | 0.000403 | 0.001491 | 0.003169 |
Bud Site | 0.002567 | 0.000640 | 0.005045 | 0.001676 | 0.001151 | 0.001270 | 0.002000 | 0.037881 | 0.001611 | 0.000965 | 0.003422 | 0.005698 |
Cell Periphery | 0.003145 | 0.002197 | 0.000662 | 0.012354 | 0.001466 | 0.001099 | 0.001170 | 0.010319 | 0.002011 | 0.001630 | 0.000933 | 0.012128 |
Cytoplasm | 0.800906* | 0.723046* | 0.760578* | 0.586109* | 0.799317* | 0.682804* | 0.591214* | 0.620169* | 0.799833* | 0.702257* | 0.670330* | 0.589893* |
Cytoplasmic Foci | 0.006311 | 0.004967 | 0.009362 | 0.039484 | 0.004393 | 0.007215 | 0.003824 | 0.032087 | 0.005016 | 0.006128 | 0.006411 | 0.038662 |
Eisosomes | 0.000001 | 0.000001 | 0.000001 | 0.000007 | 0.000001 | 0.000001 | 0.000001 | 0.000005 | 0.000001 | 0.000001 | 0.000001 | 0.000007 |
Endoplasmic Reticulum | 0.000037 | 0.000025 | 0.000032 | 0.000013 | 0.000029 | 0.000027 | 0.000025 | 0.000051 | 0.000032 | 0.000026 | 0.000028 | 0.000017 |
Endosome | 0.000048 | 0.000018 | 0.000092 | 0.000083 | 0.000036 | 0.000037 | 0.000040 | 0.000546 | 0.000040 | 0.000028 | 0.000064 | 0.000134 |
Golgi | 0.002525 | 0.000774 | 0.000557 | 0.002019 | 0.001807 | 0.001118 | 0.000328 | 0.016352 | 0.002041 | 0.000952 | 0.000435 | 0.003611 |
Lipid Particles | 0.001099 | 0.000250 | 0.000541 | 0.009514 | 0.000528 | 0.000319 | 0.000638 | 0.010122 | 0.000714 | 0.000286 | 0.000593 | 0.009582 |
Mitochondria | 0.000362 | 0.000231 | 0.000332 | 0.000071 | 0.000224 | 0.000389 | 0.000304 | 0.000060 | 0.000269 | 0.000313 | 0.000317 | 0.000070 |
Mitotic Spindle | 0.001505 | 0.000417 | 0.007615 | 0.000132 | 0.000236 | 0.000608 | 0.001770 | 0.001077 | 0.000649 | 0.000516 | 0.004500 | 0.000237 |
None | 0.015583 | 0.021991 | 0.011480 | 0.003504 | 0.009507 | 0.015060 | 0.009426 | 0.002652 | 0.011482 | 0.018411 | 0.010385 | 0.003409 |
Nuclear Periphery | 0.000165 | 0.000131 | 0.000262 | 0.000650 | 0.000132 | 0.000162 | 0.000222 | 0.000561 | 0.000143 | 0.000147 | 0.000241 | 0.000640 |
Nuclear Periphery Foci | 0.001094 | 0.000523 | 0.000665 | 0.003618 | 0.001053 | 0.000514 | 0.000325 | 0.003549 | 0.001066 | 0.000518 | 0.000484 | 0.003611 |
Nucleolus | 0.000184 | 0.000250 | 0.000165 | 0.000085 | 0.000060 | 0.000268 | 0.000111 | 0.000103 | 0.000100 | 0.000260 | 0.000136 | 0.000087 |
Nucleus | 0.147988* | 0.239611* | 0.193705* | 0.205676* | 0.172156* | 0.279938* | 0.371712* | 0.098138 | 0.164299* | 0.260444* | 0.288559* | 0.193728* |
Peroxisomes | 0.000015 | 0.000016 | 0.000033 | 0.000014 | 0.000006 | 0.000008 | 0.000024 | 0.000372 | 0.000009 | 0.000012 | 0.000028 | 0.000053 |
Vacuole | 0.010741 | 0.003473 | 0.006119 | 0.131039 | 0.005891 | 0.005309 | 0.015502 | 0.151026 | 0.007468 | 0.004422 | 0.011119 | 0.133260 |
Vacuole Periphery | 0.004754 | 0.000990 | 0.000446 | 0.000734 | 0.001537 | 0.003345 | 0.000470 | 0.010675 | 0.002583 | 0.002207 | 0.000459 | 0.001839 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.05 | 0.29 | -3.21 | -2.50 | -4.04 | -2.43 | -2.96 | -1.69 | -1.56 | -1.59 | -2.04 | -2.55 | -3.63 | -2.78 | -3.03 |
Bud Neck | 2.26 | -5.26 | -5.83 | -6.67 | -3.10 | -0.55 | -10.62 | -2.30 | -2.26 | -1.37 | 1.48 | -9.40 | -6.75 | -6.98 | -4.24 |
Bud Site | 3.76 | -3.75 | 0.35 | -4.42 | 4.47 | -0.25 | -2.02 | -1.56 | -1.56 | -1.51 | 2.47 | -4.92 | -1.79 | -2.16 | -0.70 |
Cell Periphery | 1.45 | 6.44 | -3.31 | -3.79 | -4.94 | 1.50 | 2.19 | -2.99 | -3.18 | -3.27 | 1.19 | 5.78 | -4.49 | -4.72 | -5.37 |
Cytoplasm | 8.63 | 7.52 | 13.14 | 9.58 | 10.33 | 16.55 | 34.89 | 2.69 | 0.68 | -1.67 | 17.97 | 29.70 | 13.64 | 8.47 | 4.89 |
Cytoplasmic Foci | 1.29 | -3.82 | -8.13 | -8.40 | -7.37 | -4.01 | -1.41 | -3.50 | -3.13 | -3.39 | -2.34 | -4.82 | -8.88 | -8.62 | -8.32 |
Eisosomes | 0.47 | -0.61 | -7.83 | -7.85 | -7.72 | -2.53 | -5.47 | -3.90 | -3.48 | -2.83 | -1.94 | -5.55 | -8.84 | -8.41 | -7.81 |
Endoplasmic Reticulum | 1.09 | 0.04 | 2.81 | 1.85 | 6.83 | 0.61 | -0.57 | -0.60 | -0.78 | -0.49 | 1.17 | -0.54 | 3.90 | 1.92 | 5.91 |
Endosome | 2.56 | -4.17 | -2.93 | -4.45 | -0.34 | -0.20 | -4.68 | -1.42 | -1.41 | -1.27 | 1.82 | -6.49 | -3.34 | -3.69 | -1.79 |
Golgi | 3.82 | 4.29 | 1.49 | -3.18 | -3.93 | 2.88 | 7.71 | -1.13 | -1.25 | -1.36 | 5.09 | 8.15 | -0.63 | -2.10 | -2.74 |
Lipid Particles | 3.51 | 1.29 | -5.95 | -6.79 | -6.33 | 1.34 | -2.15 | -3.24 | -3.31 | -3.05 | 3.70 | -0.95 | -6.95 | -7.35 | -6.82 |
Mitochondria | 1.49 | -0.48 | 4.13 | 5.13 | 14.40 | -1.88 | -4.64 | 4.98 | 3.62 | 8.23 | -0.80 | -3.63 | 7.62 | 5.68 | 16.53* |
Mitotic Spindle | 1.30 | -5.07 | 1.61 | 2.13 | 7.56 | -1.79 | -5.60 | -1.12 | -0.91 | 0.05 | 0.41 | -7.49 | 0.74 | 0.53 | 8.17 |
None | -2.73 | 2.15 | 6.98 | 13.02 | 13.56 | -4.49 | 1.08 | 6.34 | 8.09 | 5.86 | -5.97 | 1.93 | 12.45 | 16.53 | 16.93 |
Nuclear Periphery | 0.56 | -4.86 | -7.15 | -7.85 | -4.99 | -0.85 | -13.80 | -4.14 | -3.57 | -1.05 | -0.41 | -12.71 | -8.39 | -8.06 | -4.92 |
Nuclear Periphery Foci | 3.31 | 1.46 | -5.71 | -7.56 | -6.66 | 3.17 | 4.61 | -2.00 | -2.59 | -2.74 | 4.69 | 3.82 | -6.44 | -7.89 | -7.66 |
Nucleolus | -0.50 | -0.12 | 1.53 | 1.24 | 6.46 | -2.13 | -10.54 | -1.41 | 1.74 | 3.22 | -1.95 | -3.89 | 0.20 | 2.05 | 7.83 |
Nucleus | -11.41 | -8.15 | -2.61 | 2.93 | 1.05 | -15.93 | -30.37 | 2.22 | 6.84 | 11.73 | -18.85 | -26.95 | -1.42 | 5.14 | 8.32 |
Peroxisomes | 0.03 | -1.87 | -0.71 | -0.51 | 1.57 | -1.02 | -1.77 | -1.15 | -1.14 | -0.99 | -0.62 | -2.55 | -1.53 | -1.39 | -0.35 |
Vacuole | 4.43 | 1.28 | -8.75 | -9.20 | -8.90 | -0.30 | -11.75 | -2.44 | -2.44 | -1.89 | 3.03 | -9.74 | -9.28 | -9.44 | -8.48 |
Vacuole Periphery | 2.49 | 2.97 | 2.84 | 0.77 | -1.20 | -1.57 | 3.10 | -0.90 | -0.56 | -1.14 | 0.64 | 4.07 | 1.79 | 1.15 | -1.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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