Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 3 | 3 | 2 | 1 | – | 1 | 2 | 5 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 3 | 3 |
Bud | 0 | 3 | 0 | 0 | 2 | 1 | 1 | – | 4 | 4 | 8 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 12 | 7 | 4 | 11 | 5 | – | 2 | 7 | 4 | 5 | 2 | 6 | 5 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 137 | 221 | 246 | 193 | 204 | 240 | 209 | – | 114 | 188 | 126 | 149 | 300 | 539 | 617 | 193 | 189 | 190 | 131 | 229 | 221 | 242 | 240 | 284 |
Endoplasmic Reticulum | 0 | 1 | 2 | 3 | 5 | 8 | 9 | – | 2 | 1 | 0 | 6 | 1 | 4 | 1 | 5 | 7 | 9 | 0 | 0 | 4 | 1 | 1 | 3 |
Endosome | 0 | 0 | 1 | 2 | 10 | 27 | 14 | – | 10 | 10 | 12 | 23 | 0 | 0 | 0 | 2 | 0 | 0 | 5 | 3 | 4 | 2 | 4 | 6 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 |
Mitochondria | 1 | 10 | 0 | 7 | 2 | 39 | 35 | – | 113 | 119 | 132 | 185 | 0 | 1 | 0 | 6 | 6 | 3 | 1 | 7 | 18 | 6 | 8 | 13 |
Nucleus | 1 | 0 | 0 | 2 | 0 | 2 | 2 | – | 4 | 3 | 4 | 5 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 3 | 1 | 4 | – | 1 | 4 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 1 | 3 |
Vac/Vac Membrane | 4 | 0 | 7 | 0 | 20 | 40 | 47 | – | 24 | 19 | 25 | 40 | 0 | 2 | 9 | 1 | 1 | 3 | 1 | 2 | 1 | 5 | 2 | 4 |
Unique Cell Count | 141 | 230 | 260 | 202 | 237 | 336 | 303 | 233 | 303 | 262 | 358 | 301 | 548 | 619 | 198 | 194 | 195 | 147 | 251 | 272 | 273 | 280 | 341 | |
Labelled Cell Count | 146 | 238 | 272 | 217 | 254 | 372 | 328 | 276 | 358 | 318 | 432 | 305 | 555 | 633 | 213 | 207 | 211 | 147 | 251 | 272 | 273 | 280 | 341 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 5.1 | 7.1 | 7.0 | 7.6 | 6.2 | 6.1 | – | 5.2 | 5.3 | 5.3 | 5.2 | 6.6 | 6.4 | 6.3 | 5.4 | 6.4 | 6.4 | 7.3 | 7.3 | 7.0 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.0 | 1.8 | 1.8 | 1.5 | 1.7 | – | 1.3 | 1.0 | 1.6 | 0.9 | 0.9 | 1.0 | 1.0 | 1.1 | 1.1 | 1.3 | 1.8 | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.09 | -0.22 | -0.23 | – | -0.47 | -0.44 | -0.43 | -0.46 | -0.11 | -0.15 | -0.19 | -0.39 | -0.16 | -0.17 | 0.03 | 0.03 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | -1.8 | -0.8 | -2.0 | – | -2.5 | -1.5 | -2.0 | -2.4 | -3.0 | -3.2 | -3.7 | -1.2 | -2.2 | -1.8 |
Cytoplasm | 0.5 | -3.2 | -7.2 | -7.7 | – | -11.4 | -9.2 | -11.7 | -13.5 | 3.7 | 3.0 | 5.1 | 1.5 | 1.5 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.9 | 4.4 | 3.1 | – | 2.9 | 2.5 | 3.1 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.7 | 5.7 | – | 12.8 | 11.4 | 13.2 | 13.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.8 | 4.1 | 5.1 | – | 3.5 | 2.0 | 3.3 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1752 | 6.7615 | 6.677 | 6.0205 | 6.6394 | 6.3389 | 3.479 | 4.6568 | 4.5459 | 4.4204 | 3.5322 | 4.4189 | 0.9347 | 1.9367 | 1.6467 | 1.6167 | 1.1291 | 1.5531 |
Actin | 0.0366 | 0.0014 | 0.0253 | 0.0295 | 0.0441 | 0.0032 | 0.0444 | 0.001 | 0.0165 | 0.0067 | 0.0012 | 0.0012 | 0.0967 | 0.0019 | 0.0534 | 0.0236 | 0.0067 | 0.008 |
Bud | 0.002 | 0.0025 | 0.0017 | 0.0014 | 0.0005 | 0.0009 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.001 | 0.0005 | 0.0024 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0046 | 0.0005 | 0.0077 | 0.0024 | 0.0012 | 0.0092 | 0.0014 | 0.0025 | 0.0008 | 0.0015 | 0.0007 | 0.0013 | 0.0164 | 0.0003 | 0.0018 | 0.0013 | 0.0008 | 0.0021 |
Bud Periphery | 0.0027 | 0.0012 | 0.0015 | 0.0011 | 0.0007 | 0.0009 | 0.0003 | 0.0002 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0016 | 0.0004 | 0.0021 | 0.0003 | 0.0002 | 0.0001 |
Bud Site | 0.0111 | 0.0265 | 0.0051 | 0.0195 | 0.001 | 0.0005 | 0.0015 | 0.0307 | 0.0062 | 0.0016 | 0.0001 | 0.0002 | 0.0118 | 0.0034 | 0.0117 | 0.0004 | 0.0008 | 0.0002 |
Cell Periphery | 0.0004 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0007 | 0.0007 | 0.0008 | 0.0003 | 0.0002 | 0.0001 | 0.0008 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.6308 | 0.8542 | 0.7733 | 0.8431 | 0.5144 | 0.821 | 0.4968 | 0.9024 | 0.8279 | 0.6868 | 0.9422 | 0.8998 | 0.6627 | 0.8815 | 0.7878 | 0.8566 | 0.6615 | 0.9275 |
Cytoplasmic Foci | 0.0455 | 0.0139 | 0.0289 | 0.0284 | 0.0325 | 0.0155 | 0.0971 | 0.0078 | 0.0146 | 0.0188 | 0.0112 | 0.0136 | 0.0176 | 0.0118 | 0.0125 | 0.0067 | 0.0459 | 0.0236 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0016 | 0 | 0.0002 | 0 | 0.0003 | 0.0002 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.011 | 0.0029 | 0.0051 | 0.0022 | 0.0058 | 0.0024 | 0.0132 | 0.0024 | 0.0096 | 0.0066 | 0.0039 | 0.0061 | 0.0056 | 0.0038 | 0.0046 | 0.0031 | 0.0025 | 0.0034 |
Endosome | 0.0338 | 0.0055 | 0.0114 | 0.0135 | 0.0841 | 0.0129 | 0.0376 | 0.001 | 0.0181 | 0.0161 | 0.0047 | 0.0194 | 0.0106 | 0.0096 | 0.0173 | 0.0041 | 0.0032 | 0.01 |
Golgi | 0.0115 | 0.0009 | 0.005 | 0.0157 | 0.0508 | 0.0015 | 0.006 | 0.0005 | 0.0074 | 0.0041 | 0.0003 | 0.0014 | 0.0069 | 0.0015 | 0.0057 | 0.0028 | 0.0039 | 0.0025 |
Lipid Particles | 0.0087 | 0.0001 | 0.0043 | 0.0007 | 0.0192 | 0.0033 | 0.0149 | 0.0002 | 0.0032 | 0.003 | 0.0001 | 0.0002 | 0.0101 | 0.0012 | 0.0095 | 0.0006 | 0.0418 | 0.0013 |
Mitochondria | 0.0214 | 0.0005 | 0.0114 | 0.0052 | 0.0275 | 0.0046 | 0.0023 | 0.0001 | 0.0038 | 0.001 | 0.0001 | 0.0007 | 0.0091 | 0.0015 | 0.0061 | 0.0007 | 0.0022 | 0.0007 |
None | 0.1254 | 0.0836 | 0.0928 | 0.0312 | 0.1558 | 0.1128 | 0.239 | 0.0444 | 0.0759 | 0.185 | 0.0314 | 0.0511 | 0.1131 | 0.0749 | 0.054 | 0.0954 | 0.1943 | 0.0146 |
Nuclear Periphery | 0.0118 | 0.0005 | 0.0056 | 0.0006 | 0.007 | 0.0007 | 0.0022 | 0.0004 | 0.0032 | 0.0035 | 0.0008 | 0.0013 | 0.0057 | 0.0008 | 0.005 | 0.0007 | 0.0004 | 0.0004 |
Nucleolus | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0023 | 0.0011 | 0.0022 | 0.0002 | 0.0002 | 0.0004 | 0 | 0 | 0.0007 | 0.0005 | 0.0002 | 0 | 0.0002 | 0 |
Nucleus | 0.0049 | 0.0016 | 0.0043 | 0.0009 | 0.0044 | 0.0023 | 0.0026 | 0.0015 | 0.0017 | 0.055 | 0.0014 | 0.0012 | 0.002 | 0.0017 | 0.0023 | 0.0012 | 0.0007 | 0.0011 |
Peroxisomes | 0.0234 | 0.001 | 0.0089 | 0.002 | 0.0315 | 0.0022 | 0.015 | 0.0001 | 0.003 | 0.0019 | 0.0001 | 0.0002 | 0.0211 | 0.0008 | 0.0192 | 0.0008 | 0.0315 | 0.003 |
Punctate Nuclear | 0.0093 | 0.0005 | 0.0046 | 0.0004 | 0.0109 | 0.0012 | 0.0182 | 0.0021 | 0.0009 | 0.0051 | 0.0004 | 0.0004 | 0.003 | 0.0005 | 0.0013 | 0.0003 | 0.0027 | 0.0005 |
Vacuole | 0.003 | 0.0019 | 0.0014 | 0.0011 | 0.0026 | 0.0026 | 0.0037 | 0.0011 | 0.0034 | 0.0012 | 0.0008 | 0.0013 | 0.002 | 0.0025 | 0.0018 | 0.0006 | 0.0003 | 0.0008 |
Vacuole Periphery | 0.0015 | 0.0002 | 0.0004 | 0.0003 | 0.0018 | 0.0006 | 0.0004 | 0 | 0.0015 | 0.0003 | 0.0001 | 0.0004 | 0.0007 | 0.0005 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.1314 | 13.445 | 3.6279 | 11.7926 | 26.5913 | 20.498 | 11.7405 | 12.5116 | 10.4496 | 28.3499 |
Translational Efficiency | 1.2149 | 1.1626 | 1.7681 | 0.8441 | 1.2892 | 1.8307 | 1.1861 | 0.5059 | 0.8895 | 1.0238 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
95 | 1636 | 1601 | 1249 | 1880 | 1218 | 1745 | 1453 | 1975 | 2854 | 3346 | 2702 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 700.20 | 877.51 | 893.38 | 902.77 | 781.68 | 899.16 | 892.67 | 914.50 | 777.76 | 886.75 | 893.01 | 909.08 |
Standard Deviation | 105.44 | 126.96 | 113.87 | 124.45 | 88.00 | 113.58 | 104.30 | 126.84 | 90.61 | 121.90 | 108.99 | 125.88 |
Intensity Change Log 2 | — | 0.325649 | 0.351507 | 0.366591 | — | 0.202000 | 0.191549 | 0.226405 | — | 0.261747 | 0.269344 | 0.294344 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000205 | 0.001469 | 0.000399 | 0.008471 | 0.000638 | 0.001335 | 0.000468 | 0.005413 | 0.000617 | 0.001412 | 0.000435 | 0.006827 |
Bud Neck | 0.016405 | 0.025252 | 0.003492 | 0.013996 | 0.020904 | 0.036617 | 0.007939 | 0.020374 | 0.020688 | 0.030102 | 0.005811 | 0.017426 |
Bud Site | 0.005778 | 0.051519 | 0.009290 | 0.087385 | 0.010142 | 0.041637 | 0.015431 | 0.094815 | 0.009932 | 0.047301 | 0.012493 | 0.091380 |
Cell Periphery | 0.000241 | 0.000138 | 0.000168 | 0.000244 | 0.000094 | 0.000173 | 0.000094 | 0.000183 | 0.000101 | 0.000153 | 0.000130 | 0.000211 |
Cytoplasm | 0.382731* | 0.466037* | 0.593052* | 0.338012* | 0.492427* | 0.410929* | 0.624991* | 0.437993* | 0.487151* | 0.442519* | 0.609708* | 0.391777* |
Cytoplasmic Foci | 0.276922* | 0.261104* | 0.014568 | 0.148166 | 0.243283* | 0.258371* | 0.050661 | 0.118675 | 0.244901* | 0.259937* | 0.033392 | 0.132307 |
Eisosomes | 0.000223 | 0.000095 | 0.000026 | 0.000127 | 0.000138 | 0.000153 | 0.000040 | 0.000086 | 0.000142 | 0.000120 | 0.000034 | 0.000105 |
Endoplasmic Reticulum | 0.001703 | 0.001363 | 0.002462 | 0.003651 | 0.001707 | 0.000835 | 0.001510 | 0.001540 | 0.001707 | 0.001138 | 0.001966 | 0.002516 |
Endosome | 0.064239 | 0.023538 | 0.001666 | 0.028923 | 0.018228 | 0.029863 | 0.007241 | 0.016933 | 0.020441 | 0.026237 | 0.004574 | 0.022475 |
Golgi | 0.004237 | 0.011363 | 0.000232 | 0.008321 | 0.005459 | 0.011734 | 0.001058 | 0.008903 | 0.005400 | 0.011521 | 0.000663 | 0.008634 |
Lipid Particles | 0.017096 | 0.003168 | 0.000789 | 0.003634 | 0.006633 | 0.004039 | 0.001643 | 0.001806 | 0.007136 | 0.003540 | 0.001235 | 0.002651 |
Mitochondria | 0.010927 | 0.006671 | 0.000550 | 0.003993 | 0.005189 | 0.009841 | 0.000783 | 0.004462 | 0.005465 | 0.008024 | 0.000672 | 0.004245 |
Mitotic Spindle | 0.000301 | 0.006471 | 0.003381 | 0.022343 | 0.002723 | 0.010723 | 0.006401 | 0.029494 | 0.002607 | 0.008286 | 0.004956 | 0.026189 |
None | 0.004846 | 0.001406 | 0.004123 | 0.000592 | 0.001295 | 0.000840 | 0.002122 | 0.001187 | 0.001466 | 0.001165 | 0.003080 | 0.000912 |
Nuclear Periphery | 0.001162 | 0.000179 | 0.000906 | 0.000801 | 0.000319 | 0.000213 | 0.000718 | 0.000413 | 0.000360 | 0.000194 | 0.000808 | 0.000592 |
Nuclear Periphery Foci | 0.001036 | 0.000835 | 0.002023 | 0.002786 | 0.000394 | 0.000443 | 0.001570 | 0.001301 | 0.000425 | 0.000668 | 0.001786 | 0.001987 |
Nucleolus | 0.003686 | 0.000781 | 0.000153 | 0.000550 | 0.000804 | 0.000708 | 0.000482 | 0.000498 | 0.000942 | 0.000750 | 0.000325 | 0.000522 |
Nucleus | 0.088453 | 0.041431 | 0.300746* | 0.038518 | 0.091910 | 0.046308 | 0.126825 | 0.049465 | 0.091744 | 0.043513 | 0.210043* | 0.044405 |
Peroxisomes | 0.014676 | 0.015987 | 0.000277 | 0.026427 | 0.006634 | 0.021365 | 0.003363 | 0.035517 | 0.007021 | 0.018282 | 0.001886 | 0.031315 |
Vacuole | 0.096426 | 0.078375 | 0.061540 | 0.258106* | 0.089499 | 0.111293 | 0.145924 | 0.167882 | 0.089832 | 0.092423 | 0.105548 | 0.209588* |
Vacuole Periphery | 0.008705 | 0.002817 | 0.000156 | 0.004954 | 0.001580 | 0.002582 | 0.000734 | 0.003061 | 0.001922 | 0.002717 | 0.000457 | 0.003936 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.03 | -1.54 | -6.38 | -4.82 | -6.18 | -1.71 | 0.61 | -6.33 | -5.43 | -7.98 | -2.22 | 0.60 | -7.76 | -6.91 | -9.37 |
Bud Neck | -1.81 | 2.61 | 0.63 | 6.50 | -8.80 | -5.89 | 10.01 | 1.83 | 6.94 | -7.33 | -5.18 | 12.79 | 3.84 | 9.01 | -11.03 |
Bud Site | -15.27 | -1.54 | -15.21 | -3.07 | -14.36 | -11.45 | -1.78 | -16.22 | -6.76 | -14.97 | -17.93 | -0.73 | -21.43 | -6.72 | -20.75 |
Cell Periphery | 4.31 | 2.07 | 1.57 | -4.38 | -0.89 | -4.30 | 1.58 | -4.32 | 0.63 | -5.03 | -5.34 | -2.93 | -7.28 | -2.03 | -3.48 |
Cytoplasm | -3.00 | -5.39 | 2.18 | 13.61 | 20.61 | 7.77 | -8.90 | 7.17 | -0.93 | 15.19 | 5.29 | -8.59 | 13.43 | 8.63 | 24.68 |
Cytoplasmic Foci | 0.84 | 14.68 | 8.43 | 19.28 | -24.54 | -1.48 | 37.01 | 23.00 | 21.17 | -13.74 | -1.95 | 43.37 | 24.15 | 28.91 | -26.77 |
Eisosomes | 5.33 | 7.94 | 4.60 | -2.17 | -12.79 | -1.06 | 11.53 | 6.39 | 7.62 | -9.68 | 2.56 | 12.96 | 5.48 | 3.91 | -16.01 |
Endoplasmic Reticulum | 0.72 | -3.78 | -3.54 | -6.63 | -0.07 | 2.69 | 0.36 | 0.01 | -5.11 | -0.68 | 1.85 | -2.41 | -2.43 | -7.81 | -0.23 |
Endosome | 3.41 | 5.29 | 3.61 | 1.30 | -14.83 | -5.61 | 10.42 | 3.80 | 8.71 | -6.67 | -3.42 | 13.73 | 2.74 | 7.25 | -15.39 |
Golgi | -5.19 | 4.05 | -0.74 | 4.84 | -5.86 | -3.90 | 7.56 | -0.28 | 3.08 | -4.39 | -6.09 | 8.87 | -0.12 | 5.35 | -6.86 |
Lipid Particles | 4.39 | 5.09 | 4.28 | -1.10 | -12.05 | 3.60 | 9.95 | 9.30 | 3.70 | -2.27 | 6.32 | 11.59 | 8.76 | 2.73 | -10.65 |
Mitochondria | 0.84 | 2.09 | 1.63 | 3.90 | -3.76 | -2.95 | 8.30 | 2.35 | 4.14 | -4.34 | -2.55 | 8.64 | 3.51 | 5.60 | -5.75 |
Mitotic Spindle | -5.54 | -6.13 | -8.80 | -5.39 | -7.03 | -3.60 | -2.57 | -8.50 | -4.75 | -7.00 | -4.17 | -2.39 | -10.74 | -7.38 | -9.83 |
None | 1.49 | 0.56 | 1.76 | 2.87 | 8.31 | 2.23 | -3.63 | 0.28 | -1.48 | 3.05 | 1.45 | -5.31 | 2.19 | 0.84 | 8.28 |
Nuclear Periphery | 1.64 | -0.15 | 0.31 | -6.88 | 2.41 | 1.93 | -8.62 | -4.82 | -6.77 | 3.11 | 2.88 | -11.79 | -5.85 | -9.46 | 3.94 |
Nuclear Periphery Foci | 0.96 | -5.19 | -5.92 | -8.14 | -1.80 | -0.85 | -6.00 | -6.00 | -5.59 | 0.18 | -3.94 | -11.75 | -11.37 | -9.37 | -1.02 |
Nucleolus | 2.10 | 2.60 | 2.22 | 0.66 | -5.46 | 0.66 | 1.74 | 1.28 | 0.79 | -0.92 | 1.03 | 3.94 | 1.99 | 0.95 | -4.65 |
Nucleus | 3.49 | -16.41 | 2.83 | -2.84 | 41.28 | 12.69 | -12.54 | 6.57 | -4.98 | 17.20 | 16.56 | -30.73 | 10.46 | -6.07 | 40.19 |
Peroxisomes | -0.28 | 2.62 | -0.81 | -1.52 | -11.01 | -9.17 | 5.68 | -8.52 | -1.81 | -10.25 | -10.77 | 9.39 | -10.34 | -2.72 | -14.63 |
Vacuole | 1.41 | 0.22 | -14.83 | -31.31 | -27.87 | -5.51 | -16.71 | -21.23 | -15.12 | -5.77 | -1.86 | -12.64 | -33.85 | -33.14 | -22.90 |
Vacuole Periphery | 1.52 | 2.23 | 1.41 | -0.42 | -3.88 | -2.99 | 5.21 | -0.77 | 0.68 | -2.47 | -2.49 | 6.34 | -1.18 | 0.23 | -4.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|