Standard name
Human Ortholog
Description GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.95 0.96 0.86 0.71 0.69 – 0.49 0.62 0.48 0.42 1.0 0.98 1.0 0.97 0.97 0.97 0.9 0.92 0.82 0.89 0.86 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.08 0.05 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.12 – 0.48 0.39 0.5 0.52 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.12 0.16 – 0.1 0.06 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 3 3 2 1 – 1 2 5 3 0 1 0 0 1 0 0 0 3 3 3 3
Bud 0 3 0 0 2 1 1 – 4 4 8 7 1 1 0 0 0 0 0 0 1 1 3 4
Bud Neck 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 3 1 2 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 12 7 4 11 5 – 2 7 4 5 2 6 5 5 2 3 0 0 0 0 0 0
Cytoplasm 137 221 246 193 204 240 209 – 114 188 126 149 300 539 617 193 189 190 131 229 221 242 240 284
Endoplasmic Reticulum 0 1 2 3 5 8 9 – 2 1 0 6 1 4 1 5 7 9 0 0 4 1 1 3
Endosome 0 0 1 2 10 27 14 – 10 10 12 23 0 0 0 2 0 0 5 3 4 2 4 6
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 1 1 0 1 1 0 1 0
Mitochondria 1 10 0 7 2 39 35 – 113 119 132 185 0 1 0 6 6 3 1 7 18 6 8 13
Nucleus 1 0 0 2 0 2 2 – 4 3 4 5 1 1 1 1 0 1 0 1 0 2 3 0
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 1 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 3 1 4 – 1 4 2 5 0 0 0 0 0 1 1 0 3 1 1 3
Vac/Vac Membrane 4 0 7 0 20 40 47 – 24 19 25 40 0 2 9 1 1 3 1 2 1 5 2 4
Unique Cell Count 141 230 260 202 237 336 303 233 303 262 358 301 548 619 198 194 195 147 251 272 273 280 341
Labelled Cell Count 146 238 272 217 254 372 328 276 358 318 432 305 555 633 213 207 211 147 251 272 273 280 341


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.1 7.1 7.0 7.6 6.2 6.1 – 5.2 5.3 5.3 5.2 6.6 6.4 6.3 5.4 6.4 6.4 7.3 7.3 7.0
Std Deviation (1e-4) 0.8 0.7 1.0 1.8 1.8 1.5 1.7 – 1.3 1.0 1.6 0.9 0.9 1.0 1.0 1.1 1.1 1.3 1.8 1.4 1.2
Intensity Change (Log2) – – – -0.04 0.09 -0.22 -0.23 – -0.47 -0.44 -0.43 -0.46 -0.11 -0.15 -0.19 -0.39 -0.16 -0.17 0.03 0.03 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -1.8 -0.8 -2.0 – -2.5 -1.5 -2.0 -2.4 -3.0 -3.2 -3.7 -1.2 -2.2 -1.8
Cytoplasm 0.5 -3.2 -7.2 -7.7 – -11.4 -9.2 -11.7 -13.5 3.7 3.0 5.1 1.5 1.5 1.5
Endoplasmic Reticulum 0 0 0 1.9 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.9 4.4 3.1 – 2.9 2.5 3.1 3.8 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.7 5.7 – 12.8 11.4 13.2 13.8 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.8 4.1 5.1 – 3.5 2.0 3.3 3.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1752 6.7615 6.677 6.0205 6.6394 6.3389 3.479 4.6568 4.5459 4.4204 3.5322 4.4189 0.9347 1.9367 1.6467 1.6167 1.1291 1.5531
Actin 0.0366 0.0014 0.0253 0.0295 0.0441 0.0032 0.0444 0.001 0.0165 0.0067 0.0012 0.0012 0.0967 0.0019 0.0534 0.0236 0.0067 0.008
Bud 0.002 0.0025 0.0017 0.0014 0.0005 0.0009 0.0003 0.0004 0.0005 0.0004 0.0002 0.0001 0.001 0.0005 0.0024 0.0003 0.0002 0.0001
Bud Neck 0.0046 0.0005 0.0077 0.0024 0.0012 0.0092 0.0014 0.0025 0.0008 0.0015 0.0007 0.0013 0.0164 0.0003 0.0018 0.0013 0.0008 0.0021
Bud Periphery 0.0027 0.0012 0.0015 0.0011 0.0007 0.0009 0.0003 0.0002 0.0005 0.0005 0.0002 0.0001 0.0016 0.0004 0.0021 0.0003 0.0002 0.0001
Bud Site 0.0111 0.0265 0.0051 0.0195 0.001 0.0005 0.0015 0.0307 0.0062 0.0016 0.0001 0.0002 0.0118 0.0034 0.0117 0.0004 0.0008 0.0002
Cell Periphery 0.0004 0.0005 0.0005 0.0005 0.0003 0.0002 0.0007 0.0007 0.0008 0.0003 0.0002 0.0001 0.0008 0.0003 0.0004 0.0001 0.0001 0
Cytoplasm 0.6308 0.8542 0.7733 0.8431 0.5144 0.821 0.4968 0.9024 0.8279 0.6868 0.9422 0.8998 0.6627 0.8815 0.7878 0.8566 0.6615 0.9275
Cytoplasmic Foci 0.0455 0.0139 0.0289 0.0284 0.0325 0.0155 0.0971 0.0078 0.0146 0.0188 0.0112 0.0136 0.0176 0.0118 0.0125 0.0067 0.0459 0.0236
Eisosomes 0.0003 0 0.0001 0.0002 0.0016 0 0.0002 0 0.0003 0.0002 0 0 0.0008 0 0.0003 0.0001 0.0001 0
Endoplasmic Reticulum 0.011 0.0029 0.0051 0.0022 0.0058 0.0024 0.0132 0.0024 0.0096 0.0066 0.0039 0.0061 0.0056 0.0038 0.0046 0.0031 0.0025 0.0034
Endosome 0.0338 0.0055 0.0114 0.0135 0.0841 0.0129 0.0376 0.001 0.0181 0.0161 0.0047 0.0194 0.0106 0.0096 0.0173 0.0041 0.0032 0.01
Golgi 0.0115 0.0009 0.005 0.0157 0.0508 0.0015 0.006 0.0005 0.0074 0.0041 0.0003 0.0014 0.0069 0.0015 0.0057 0.0028 0.0039 0.0025
Lipid Particles 0.0087 0.0001 0.0043 0.0007 0.0192 0.0033 0.0149 0.0002 0.0032 0.003 0.0001 0.0002 0.0101 0.0012 0.0095 0.0006 0.0418 0.0013
Mitochondria 0.0214 0.0005 0.0114 0.0052 0.0275 0.0046 0.0023 0.0001 0.0038 0.001 0.0001 0.0007 0.0091 0.0015 0.0061 0.0007 0.0022 0.0007
None 0.1254 0.0836 0.0928 0.0312 0.1558 0.1128 0.239 0.0444 0.0759 0.185 0.0314 0.0511 0.1131 0.0749 0.054 0.0954 0.1943 0.0146
Nuclear Periphery 0.0118 0.0005 0.0056 0.0006 0.007 0.0007 0.0022 0.0004 0.0032 0.0035 0.0008 0.0013 0.0057 0.0008 0.005 0.0007 0.0004 0.0004
Nucleolus 0.0003 0.0001 0.0008 0.0001 0.0023 0.0011 0.0022 0.0002 0.0002 0.0004 0 0 0.0007 0.0005 0.0002 0 0.0002 0
Nucleus 0.0049 0.0016 0.0043 0.0009 0.0044 0.0023 0.0026 0.0015 0.0017 0.055 0.0014 0.0012 0.002 0.0017 0.0023 0.0012 0.0007 0.0011
Peroxisomes 0.0234 0.001 0.0089 0.002 0.0315 0.0022 0.015 0.0001 0.003 0.0019 0.0001 0.0002 0.0211 0.0008 0.0192 0.0008 0.0315 0.003
Punctate Nuclear 0.0093 0.0005 0.0046 0.0004 0.0109 0.0012 0.0182 0.0021 0.0009 0.0051 0.0004 0.0004 0.003 0.0005 0.0013 0.0003 0.0027 0.0005
Vacuole 0.003 0.0019 0.0014 0.0011 0.0026 0.0026 0.0037 0.0011 0.0034 0.0012 0.0008 0.0013 0.002 0.0025 0.0018 0.0006 0.0003 0.0008
Vacuole Periphery 0.0015 0.0002 0.0004 0.0003 0.0018 0.0006 0.0004 0 0.0015 0.0003 0.0001 0.0004 0.0007 0.0005 0.0007 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.1314 13.445 3.6279 11.7926 26.5913 20.498 11.7405 12.5116 10.4496 28.3499
Translational Efficiency 1.2149 1.1626 1.7681 0.8441 1.2892 1.8307 1.1861 0.5059 0.8895 1.0238

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cin2

Cin2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cin2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available