Standard name | |
---|---|
Human Ortholog | |
Description | Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 4 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 5 | 6 | 9 | 11 | 7 | 11 | 19 | 8 | 15 | 3 | 6 | 1 | 17 | 36 | 11 | 10 | 11 | 0 | 2 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 1 | 2 | 3 | 7 | 11 | 15 | 5 | 3 | 6 | 2 | 5 | 1 | 2 | 5 | 2 | 3 | 0 | 0 | 0 | 1 | 1 | 9 | 9 |
Golgi | 0 | 1 | 8 | 4 | 9 | 5 | 6 | 22 | 3 | 6 | 0 | 2 | 2 | 15 | 6 | 5 | 8 | 11 | 0 | 0 | 0 | 0 | 3 | 3 |
Mitochondria | 0 | 0 | 0 | 8 | 6 | 9 | 13 | 3 | 26 | 10 | 16 | 9 | 2 | 16 | 12 | 10 | 9 | 10 | 0 | 0 | 0 | 0 | 0 | 5 |
Nucleus | 44 | 69 | 86 | 12 | 7 | 12 | 29 | 40 | 25 | 36 | 20 | 45 | 8 | 4 | 3 | 12 | 14 | 28 | 64 | 96 | 117 | 121 | 84 | 53 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 0 | 1 | 3 |
Nucleolus | 15 | 25 | 40 | 191 | 200 | 217 | 250 | 285 | 162 | 173 | 91 | 136 | 49 | 211 | 276 | 63 | 91 | 90 | 47 | 42 | 104 | 72 | 131 | 146 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 10 | 12 | 7 | 9 | 1 | 2 | 0 | 4 | 0 | 1 | 12 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 16 | 21 |
Vac/Vac Membrane | 13 | 26 | 35 | 14 | 17 | 34 | 41 | 52 | 13 | 29 | 16 | 21 | 7 | 14 | 13 | 23 | 43 | 42 | 7 | 13 | 14 | 17 | 38 | 67 |
Unique Cell Count | 70 | 113 | 164 | 222 | 242 | 272 | 333 | 393 | 210 | 249 | 125 | 210 | 66 | 250 | 336 | 114 | 156 | 174 | 126 | 161 | 252 | 221 | 295 | 330 |
Labelled Cell Count | 72 | 127 | 177 | 243 | 268 | 309 | 375 | 436 | 243 | 278 | 149 | 232 | 70 | 285 | 367 | 131 | 183 | 198 | 126 | 161 | 252 | 221 | 295 | 330 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 41.6 | 32.6 | 37.8 | 38.4 | 33.5 | 30.3 | 27.9 | 28.1 | 25.2 | 26.2 | 24.5 | 24.9 | 41.6 | 39.6 | 36.9 | 31.0 | 37.5 | 41.7 | 46.4 | 45.2 | 42.0 |
Std Deviation (1e-4) | 11.0 | 10.1 | 10.8 | 11.8 | 10.5 | 8.5 | 8.2 | 7.9 | 7.6 | 7.8 | 8.4 | 7.7 | 12.0 | 12.6 | 14.6 | 12.7 | 14.6 | 16.5 | 15.8 | 13.8 | 16.5 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.17 | -0.32 | -0.44 | -0.43 | -0.58 | -0.53 | -0.62 | -0.6 | 0.14 | 0.07 | -0.03 | -0.29 | -0.01 | 0.14 | 0.3 | 0.26 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 0.4 | 0 | -0.2 | 0.6 | 0.1 | 1.1 | 0 | -0.4 | 0 | 1.4 | 2.7 | 2.1 | 1.1 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.7 | -0.6 | 0 | 0 | 0.3 | 0 | -1.4 | 0 | 0 | 0 | 0.5 | 0 | -0.2 | 0.1 | 0.6 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.7 | 0 | 4.7 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 |
Nucleus | -10.5 | -11.7 | -11.6 | -10.9 | -10.9 | -8.5 | -8.3 | -6.4 | -6.2 | -5.6 | -12.3 | -14.1 | -7.2 | -8.4 | -7.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 12.2 | 11.7 | 11.4 | 10.8 | 10.5 | 10.2 | 9.0 | 8.2 | 7.8 | 7.0 | 12.2 | 12.6 | 5.2 | 6.2 | 5.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.4 | -4.2 | -2.5 | -2.6 | -2.4 | -4.3 | -2.7 | -1.9 | -3.1 | -1.9 | -4.8 | -6.2 | -0.2 | 1.3 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 37.7572 | 43.2088 | 38.7264 | 33.8092 | 38.4079 | 39.2132 | 32.9034 | 46.8859 | 38.2564 | 34.9174 | 34.3236 | 40.5921 | 37.0999 | 45.2592 | 39.0264 | 37.9545 | 36.1563 | 41.1705 |
Actin | 0.0075 | 0 | 0.0047 | 0.0003 | 0.0004 | 0.0002 | 0.0055 | 0.0002 | 0.0103 | 0.0007 | 0.0014 | 0.0002 | 0.0105 | 0 | 0.0112 | 0.0012 | 0.0002 | 0.0001 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0013 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0004 |
Bud Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0008 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0002 |
Bud Site | 0.0007 | 0.0001 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0066 | 0.0001 | 0.0006 | 0.0004 | 0.0007 | 0.0001 | 0.0008 | 0 | 0.0025 | 0.0003 | 0.0001 | 0 |
Cell Periphery | 0.0007 | 0.0007 | 0.0005 | 0.0005 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0007 | 0.0002 |
Cytoplasm | 0.002 | 0.0002 | 0.0008 | 0.0016 | 0.0002 | 0.0001 | 0.0111 | 0.0001 | 0.0023 | 0.001 | 0.0018 | 0.0001 | 0.0072 | 0 | 0.0059 | 0.0005 | 0.0038 | 0.0001 |
Cytoplasmic Foci | 0.0101 | 0.0001 | 0.004 | 0.0492 | 0 | 0.0038 | 0.0103 | 0 | 0.0039 | 0.0002 | 0.0002 | 0 | 0.0156 | 0 | 0.0099 | 0.0032 | 0.0455 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0012 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0023 | 0 | 0.0024 | 0.0001 | 0.0004 | 0.0002 | 0.0011 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 |
Endosome | 0.0103 | 0.0001 | 0.0029 | 0.0136 | 0.0001 | 0.0018 | 0.0152 | 0 | 0.0146 | 0.0003 | 0.002 | 0.0002 | 0.0042 | 0 | 0.0068 | 0.0023 | 0.001 | 0 |
Golgi | 0.0017 | 0 | 0.0027 | 0.0028 | 0 | 0.0006 | 0.0041 | 0 | 0.0055 | 0 | 0.0002 | 0 | 0.0018 | 0 | 0.0027 | 0.0024 | 0.0002 | 0 |
Lipid Particles | 0.0055 | 0.0003 | 0.0011 | 0.014 | 0.0001 | 0.0024 | 0.0114 | 0.0001 | 0.0022 | 0.0001 | 0.0005 | 0.0001 | 0.0104 | 0 | 0.0039 | 0.027 | 0.0076 | 0 |
Mitochondria | 0.0043 | 0.0003 | 0.0013 | 0.0048 | 0.001 | 0.0011 | 0.0054 | 0.0012 | 0.0025 | 0.0038 | 0.0129 | 0.0014 | 0.0034 | 0.0008 | 0.0221 | 0.0096 | 0.003 | 0.0024 |
None | 0.0025 | 0 | 0.0002 | 0.0039 | 0.0001 | 0 | 0.0117 | 0.0001 | 0.0012 | 0.0002 | 0.0007 | 0 | 0.0023 | 0 | 0.0016 | 0.0003 | 0.0039 | 0 |
Nuclear Periphery | 0.027 | 0.0126 | 0.004 | 0.0598 | 0.0731 | 0.0429 | 0.0451 | 0.0425 | 0.0119 | 0.0311 | 0.0839 | 0.0499 | 0.0154 | 0.0045 | 0.0025 | 0.0213 | 0.0476 | 0.0045 |
Nucleolus | 0.5849 | 0.5505 | 0.7167 | 0.5677 | 0.3416 | 0.5669 | 0.2764 | 0.4556 | 0.5122 | 0.2737 | 0.1509 | 0.4004 | 0.4184 | 0.4385 | 0.5723 | 0.5916 | 0.3522 | 0.4517 |
Nucleus | 0.3205 | 0.4269 | 0.2555 | 0.2685 | 0.5734 | 0.3702 | 0.534 | 0.4961 | 0.4073 | 0.6705 | 0.7276 | 0.5355 | 0.4893 | 0.549 | 0.35 | 0.2986 | 0.5085 | 0.5371 |
Peroxisomes | 0.0007 | 0 | 0.0002 | 0.0014 | 0 | 0.0006 | 0.0046 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0026 | 0 | 0.0016 | 0.0304 | 0.0004 | 0 |
Punctate Nuclear | 0.01 | 0.0021 | 0.0011 | 0.0034 | 0.001 | 0.001 | 0.0406 | 0.0006 | 0.019 | 0.0105 | 0.0034 | 0.0002 | 0.0118 | 0.0002 | 0.0012 | 0.0036 | 0.0072 | 0.0002 |
Vacuole | 0.0038 | 0.0043 | 0.0017 | 0.003 | 0.002 | 0.0016 | 0.0064 | 0.0005 | 0.0011 | 0.0021 | 0.0031 | 0.0019 | 0.0013 | 0.0014 | 0.0021 | 0.002 | 0.0033 | 0.0007 |
Vacuole Periphery | 0.0057 | 0.0011 | 0.0012 | 0.0044 | 0.0055 | 0.0053 | 0.0075 | 0.0023 | 0.0017 | 0.0031 | 0.0084 | 0.0085 | 0.0018 | 0.005 | 0.0019 | 0.0046 | 0.0139 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 219.0362 | 187.1174 | 145.7917 | 172.6736 | 149.2644 | 134.487 | 147.3012 | 143.655 | 133.5478 | 171.6951 |
Translational Efficiency | 1.2235 | 1.2952 | 1.4962 | 1.3029 | 1.4138 | 1.6397 | 1.5001 | 1.1173 | 1.1671 | 1.1907 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1350 | 1084 | 1178 | 1903 | 1995 | 1999 | 1455 | 1786 | 3345 | 3083 | 2633 | 3689 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1598.14 | 2599.52 | 4085.30 | 3908.11 | 2036.78 | 2469.07 | 3593.83 | 3727.52 | 1859.75 | 2514.94 | 3813.71 | 3820.68 |
Standard Deviation | 417.24 | 640.46 | 873.14 | 998.55 | 479.23 | 649.49 | 880.68 | 914.76 | 503.53 | 649.33 | 910.71 | 963.14 |
Intensity Change Log 2 | — | 0.701851 | 1.354048 | 1.290077 | — | 0.277678 | 0.819232 | 0.871926 | — | 0.479661 | 1.079019 | 1.070824 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001053 | 0.000967 | 0.001739 | 0.000870 | 0.000710 | 0.000720 | 0.001355 | 0.001224 | 0.000849 | 0.000807 | 0.001527 | 0.001042 |
Bud Neck | 0.015235 | 0.019591 | 0.026154 | 0.024646 | 0.016247 | 0.016578 | 0.026099 | 0.017001 | 0.015838 | 0.017638 | 0.026123 | 0.020945 |
Bud Site | 0.000857 | 0.000912 | 0.001231 | 0.003542 | 0.001191 | 0.001512 | 0.001826 | 0.001048 | 0.001056 | 0.001301 | 0.001560 | 0.002334 |
Cell Periphery | 0.000137 | 0.000077 | 0.000164 | 0.000237 | 0.000094 | 0.000105 | 0.000194 | 0.000130 | 0.000111 | 0.000095 | 0.000181 | 0.000185 |
Cytoplasm | 0.002010 | 0.000488 | 0.001215 | 0.003270 | 0.000995 | 0.001871 | 0.003130 | 0.001068 | 0.001405 | 0.001385 | 0.002273 | 0.002204 |
Cytoplasmic Foci | 0.000146 | 0.000528 | 0.001932 | 0.004641 | 0.000937 | 0.000840 | 0.005003 | 0.002167 | 0.000618 | 0.000730 | 0.003629 | 0.003443 |
Eisosomes | 0.000036 | 0.000035 | 0.000039 | 0.000036 | 0.000036 | 0.000030 | 0.000038 | 0.000037 | 0.000036 | 0.000032 | 0.000038 | 0.000036 |
Endoplasmic Reticulum | 0.001833 | 0.000509 | 0.000448 | 0.000411 | 0.000651 | 0.000625 | 0.000385 | 0.000361 | 0.001128 | 0.000584 | 0.000413 | 0.000387 |
Endosome | 0.000200 | 0.000113 | 0.000123 | 0.001778 | 0.000195 | 0.000267 | 0.001254 | 0.000187 | 0.000197 | 0.000213 | 0.000748 | 0.001008 |
Golgi | 0.000121 | 0.000232 | 0.000561 | 0.001771 | 0.000292 | 0.000326 | 0.000739 | 0.000778 | 0.000223 | 0.000293 | 0.000659 | 0.001290 |
Lipid Particles | 0.000028 | 0.000045 | 0.000054 | 0.000223 | 0.000031 | 0.000079 | 0.000073 | 0.000066 | 0.000030 | 0.000067 | 0.000065 | 0.000147 |
Mitochondria | 0.002351 | 0.002916 | 0.007088 | 0.006446 | 0.002380 | 0.002160 | 0.005777 | 0.005680 | 0.002368 | 0.002426 | 0.006364 | 0.006075 |
Mitotic Spindle | 0.000509 | 0.000974 | 0.003000 | 0.036584 | 0.005059 | 0.000780 | 0.007620 | 0.008968 | 0.003223 | 0.000848 | 0.005553 | 0.023214 |
None | 0.004590 | 0.001020 | 0.001134 | 0.001769 | 0.001284 | 0.002435 | 0.002137 | 0.001198 | 0.002618 | 0.001937 | 0.001688 | 0.001492 |
Nuclear Periphery | 0.002366 | 0.001443 | 0.021951 | 0.032029 | 0.002917 | 0.002406 | 0.012226 | 0.047442 | 0.002695 | 0.002068 | 0.016577 | 0.039491 |
Nuclear Periphery Foci | 0.000845 | 0.000669 | 0.002748 | 0.012353 | 0.001316 | 0.001216 | 0.001789 | 0.010805 | 0.001126 | 0.001024 | 0.002218 | 0.011603 |
Nucleolus | 0.228240* | 0.372629* | 0.412892* | 0.488837* | 0.384013* | 0.309569* | 0.394444* | 0.527693* | 0.321145* | 0.331741* | 0.402698* | 0.507649* |
Nucleus | 0.736454* | 0.591190* | 0.502778* | 0.349292* | 0.575408* | 0.653713* | 0.522640* | 0.354200* | 0.640404* | 0.631730* | 0.513754* | 0.351668* |
Peroxisomes | 0.001029 | 0.002767 | 0.006236 | 0.007267 | 0.004063 | 0.001345 | 0.006212 | 0.007402 | 0.002839 | 0.001845 | 0.006223 | 0.007333 |
Vacuole | 0.000825 | 0.000980 | 0.001762 | 0.005333 | 0.000719 | 0.001149 | 0.002048 | 0.002101 | 0.000762 | 0.001090 | 0.001920 | 0.003768 |
Vacuole Periphery | 0.001135 | 0.001916 | 0.006754 | 0.018667 | 0.001461 | 0.002272 | 0.005011 | 0.010443 | 0.001329 | 0.002147 | 0.005790 | 0.014685 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.37 | -12.03 | 3.57 | 1.88 | 16.00* | -0.04 | -17.98 | -14.11 | -13.88 | 3.43 | 1.65 | -20.95 | -7.99 | -9.56 | 14.30 |
Bud Neck | -4.22 | -10.49 | -14.53 | -8.88 | -2.75 | -0.76 | -12.43 | -4.27 | -3.52 | 8.67 | -3.19 | -16.14 | -13.74 | -10.18 | 3.94 |
Bud Site | -0.90 | -4.53 | -11.04 | -10.84 | -8.58 | -0.43 | -9.21 | -3.01 | -0.70 | 5.32 | -0.62 | -9.88 | -11.05 | -6.02 | -4.60 |
Cell Periphery | 2.79 | -2.82 | -5.33 | -8.44 | -3.70 | -0.47 | -6.55 | -4.13 | -2.56 | 4.18 | 1.15 | -7.09 | -7.04 | -7.67 | -0.27 |
Cytoplasm | 2.70 | 1.43 | -2.28 | -7.83 | -5.89 | -2.44 | -4.61 | -1.55 | 1.09 | 3.40 | 0.13 | -2.44 | -3.39 | -3.75 | -0.86 |
Cytoplasmic Foci | -4.29 | -6.51 | -8.16 | -7.67 | -5.11 | 0.33 | -5.00 | -4.46 | -4.05 | 3.06 | -0.52 | -6.28 | -9.08 | -8.33 | -0.25 |
Eisosomes | 0.15 | -2.40 | -1.41 | -1.71 | 1.27 | 4.70 | -3.99 | -1.73 | -5.43 | 2.01 | 3.73 | -4.51 | -2.21 | -5.71 | 2.41 |
Endoplasmic Reticulum | 8.12 | 8.79 | 9.08 | 2.09 | 0.75 | 0.92 | 7.47 | 8.97 | 8.41 | 2.08 | 7.78 | 10.48 | 11.04 | 6.67 | 1.41 |
Endosome | 2.58 | 2.87 | -3.10 | -3.31 | -3.28 | -1.10 | -1.52 | 0.19 | 1.21 | 1.53 | -0.41 | -1.40 | -3.07 | -2.93 | -0.77 |
Golgi | -4.39 | -15.53 | -17.08 | -15.28 | -11.61 | -0.27 | -4.80 | -13.96 | -4.90 | -0.46 | -1.11 | -8.30 | -20.24 | -12.31 | -7.89 |
Lipid Particles | -1.71 | -3.82 | -4.90 | -4.18 | -3.94 | -1.31 | -4.79 | -4.69 | -0.20 | 0.74 | -1.67 | -6.05 | -5.77 | -3.10 | -3.40 |
Mitochondria | -3.10 | -17.60 | -15.08 | -11.45 | 1.47 | 1.64 | -15.64 | -13.81 | -15.30 | 0.76 | -0.74 | -23.35 | -20.22 | -19.41 | 1.29 |
Mitotic Spindle | -0.45 | -3.05 | -13.45 | -13.45 | -10.18 | 4.50 | -2.27 | -3.49 | -7.15 | -0.80 | 3.86 | -3.44 | -12.80 | -15.14 | -8.80 |
None | 3.19 | 2.98 | 2.12 | -7.18 | -6.67 | -2.31 | -4.77 | -0.31 | 2.20 | 4.29 | 1.27 | 1.71 | 1.81 | 0.42 | 0.28 |
Nuclear Periphery | 2.12 | -8.72 | -11.55 | -12.21 | -1.64 | 0.68 | -4.90 | -12.73 | -12.92 | -9.85 | 1.28 | -9.76 | -16.88 | -17.28 | -8.88 |
Nuclear Periphery Foci | 0.57 | -4.95 | -9.08 | -9.44 | -7.13 | -0.03 | -0.76 | -10.75 | -9.93 | -10.09 | 0.11 | -3.81 | -13.78 | -13.14 | -12.04 |
Nucleolus | -11.94 | -15.00 | -22.57* | -7.22 | -4.76 | 7.85 | 2.29 | -12.80 | -20.43* | -13.68 | -1.27 | -7.05 | -22.24 | -20.23 | -13.07 |
Nucleus | 12.21 | 19.96 | 37.19 | 19.13 | 12.00 | -8.24 | 3.01 | 21.42 | 29.75 | 15.89 | 1.11 | 14.09 | 38.29 | 35.97 | 19.89 |
Peroxisomes | -9.43 | -10.52 | -19.76 | -13.04 | -1.94 | 9.43 | -5.48 | -8.07 | -17.46 | -1.56 | 5.28 | -10.40 | -16.39 | -22.83 | -2.46 |
Vacuole | -1.19 | -5.62 | -12.18 | -10.72 | -9.15 | -1.89 | -5.05 | -10.39 | -4.70 | 1.05 | -2.20 | -6.78 | -14.82 | -11.38 | -5.72 |
Vacuole Periphery | -2.99 | -8.96 | -12.52 | -11.65 | -6.90 | -1.84 | -4.86 | -13.06 | -10.32 | -6.84 | -2.80 | -9.43 | -17.06 | -14.84 | -9.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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