Standard name | |
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Human Ortholog | |
Description | Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | 1 | 5 | 5 | 3 | 8 | 7 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | – | 0 | 8 | 19 | 13 | 25 | 7 | 8 | 5 | 7 | 4 | 4 | 16 | 24 | 6 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Golgi | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | – | 0 | 2 | 1 | 15 | 44 | 19 | 60 | 45 | 79 | 45 | 1 | 1 | 1 | 6 | 0 | 3 | 2 | 0 | 0 | 0 | 3 | 4 |
Nucleus | 259 | – | 38 | 134 | 250 | 226 | 357 | 294 | 307 | 276 | 189 | 225 | 166 | 208 | 195 | 207 | 107 | 177 | 252 | 41 | 58 | 134 | 198 | 238 |
Nuclear Periphery | 0 | – | 0 | 0 | 3 | 2 | 4 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 48 | – | 5 | 29 | 13 | 32 | 73 | 62 | 110 | 64 | 81 | 84 | 19 | 21 | 23 | 15 | 11 | 19 | 23 | 1 | 1 | 17 | 50 | 67 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 1 | 1 | 2 | 4 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 |
Vac/Vac Membrane | 1 | – | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 2 | 0 | 7 | 5 | 3 | 5 | 2 | 1 | 4 | 20 | 32 |
Unique Cell Count | 278 | 41 | 150 | 276 | 252 | 432 | 339 | 379 | 317 | 258 | 270 | 178 | 235 | 229 | 235 | 121 | 194 | 291 | 46 | 64 | 164 | 285 | 357 | |
Labelled Cell Count | 317 | 43 | 174 | 288 | 290 | 513 | 394 | 493 | 403 | 366 | 372 | 193 | 252 | 252 | 250 | 132 | 206 | 291 | 46 | 64 | 164 | 285 | 357 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | – | 8.1 | 7.7 | 8.5 | 6.9 | 6.3 | 6.7 | 6.0 | 6.2 | 5.6 | 5.9 | 10.0 | 9.9 | 9.3 | 12.5 | 13.7 | 13.2 | 8.6 | 9.0 | 9.7 |
Std Deviation (1e-4) | 2.8 | – | 1.6 | 1.6 | 2.3 | 1.9 | 1.7 | 1.5 | 1.7 | 1.7 | 1.8 | 1.5 | 2.4 | 3.1 | 2.5 | 4.0 | 3.9 | 3.1 | 2.1 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.07 | -0.23 | -0.36 | -0.27 | -0.43 | -0.39 | -0.55 | -0.47 | 0.3 | 0.28 | 0.19 | 0.62 | 0.75 | 0.7 | 0.08 | 0.14 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.6 | 4.1 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.6 | -0.4 | -0.6 | -1.7 | -1.1 | -1.9 | -1.0 | -2.7 | -1.5 | 0.1 | -0.8 | -1.3 | -0.9 | -0.8 | -0.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.1 | 0 | 0.1 | 0.8 | 1.0 | 2.3 | 1.2 | 2.5 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.1886 | 5.9966 | 5.7259 | 5.475 | 4.8931 | 5.465 | 4.6731 | 5.9663 | 5.1413 | 4.4302 | 4.183 | 4.8718 | 4.2034 | 5.0782 | 4.5405 | 4.405 | 3.0204 | 4.0859 |
Actin | 0.0067 | 0.0011 | 0.0009 | 0.0356 | 0.0007 | 0.0012 | 0.0014 | 0 | 0.0001 | 0 | 0 | 0.0012 | 0.0187 | 0 | 0.0069 | 0 | 0.0015 | 0.0005 |
Bud | 0.0006 | 0.001 | 0.0005 | 0.0028 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0002 |
Bud Neck | 0.0011 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0006 | 0.0074 | 0 | 0.0002 | 0 | 0.0006 | 0.0005 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 |
Bud Periphery | 0.001 | 0.0007 | 0.0004 | 0.0033 | 0.0003 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0009 |
Bud Site | 0.002 | 0.003 | 0.0011 | 0.0143 | 0.0003 | 0.0001 | 0.0018 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0008 | 0.0005 | 0 | 0.0002 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.004 | 0.0048 | 0.0021 | 0.0021 | 0.0014 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0012 | 0.0151 | 0.0134 | 0 | 0.0001 | 0.0006 |
Cytoplasmic Foci | 0.0121 | 0.0021 | 0.0102 | 0.0342 | 0.0055 | 0.0004 | 0.0034 | 0 | 0 | 0 | 0 | 0 | 0.0175 | 0.0001 | 0.002 | 0 | 0.0042 | 0.0002 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0015 | 0.002 | 0.0008 | 0.0003 | 0.0016 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0019 | 0 | 0.0001 | 0 | 0.0001 | 0.0006 |
Endosome | 0.0142 | 0.0075 | 0.0104 | 0.0107 | 0.0309 | 0.0012 | 0.0039 | 0 | 0 | 0 | 0 | 0.0008 | 0.0117 | 0.0001 | 0.0002 | 0 | 0.0215 | 0.0066 |
Golgi | 0.0043 | 0.0012 | 0.0026 | 0.0056 | 0.0037 | 0.0003 | 0.0007 | 0 | 0 | 0 | 0 | 0.0001 | 0.0062 | 0 | 0.0007 | 0 | 0.0421 | 0.0003 |
Lipid Particles | 0.0105 | 0.0004 | 0.0017 | 0.0048 | 0.0352 | 0.0014 | 0.0069 | 0 | 0 | 0 | 0 | 0.0001 | 0.0044 | 0 | 0.001 | 0 | 0.0031 | 0.0002 |
Mitochondria | 0.008 | 0.0051 | 0.0041 | 0.0012 | 0.0091 | 0.0011 | 0.002 | 0 | 0.0003 | 0 | 0.0004 | 0.0002 | 0.0056 | 0.0004 | 0.0006 | 0.0001 | 0.0898 | 0.0032 |
None | 0.0068 | 0.0008 | 0.0009 | 0.0003 | 0.0006 | 0.0002 | 0.0029 | 0 | 0 | 0 | 0 | 0.0003 | 0.004 | 0 | 0 | 0 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0154 | 0.0032 | 0.0027 | 0.0005 | 0.0044 | 0.0127 | 0.0024 | 0.0002 | 0.0003 | 0.0001 | 0.0017 | 0.0019 | 0.0067 | 0.0007 | 0.0004 | 0.0001 | 0.0046 | 0.0207 |
Nucleolus | 0.0216 | 0.01 | 0.0102 | 0.0067 | 0.1373 | 0.0707 | 0.0178 | 0.0099 | 0.0102 | 0.0416 | 0.2127 | 0.0648 | 0.0168 | 0.0199 | 0.0065 | 0.0039 | 0.0742 | 0.043 |
Nucleus | 0.8571 | 0.9515 | 0.9368 | 0.8681 | 0.749 | 0.9037 | 0.9363 | 0.9897 | 0.9881 | 0.958 | 0.7832 | 0.9278 | 0.8916 | 0.9618 | 0.9585 | 0.996 | 0.747 | 0.9144 |
Peroxisomes | 0.01 | 0.0004 | 0.0052 | 0.0074 | 0.0161 | 0.0006 | 0.0035 | 0 | 0 | 0 | 0 | 0 | 0.0023 | 0 | 0.0079 | 0 | 0.0022 | 0.0001 |
Punctate Nuclear | 0.0155 | 0.0028 | 0.0069 | 0.0014 | 0.0015 | 0.0047 | 0.0067 | 0.0001 | 0.0002 | 0.0003 | 0.0009 | 0.0012 | 0.0027 | 0.0001 | 0.0001 | 0 | 0.0069 | 0.0007 |
Vacuole | 0.0037 | 0.0014 | 0.0017 | 0.0002 | 0.001 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.003 | 0.0002 | 0.0001 | 0 | 0.0006 | 0.0024 |
Vacuole Periphery | 0.0034 | 0.0006 | 0.0008 | 0.0001 | 0.0009 | 0.0004 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0026 | 0.0001 | 0 | 0 | 0.0012 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.6464 | 40.8614 | 56.4111 | 54.1019 | 56.5078 | 52.0587 | 56.2076 | 66.4696 | 61.9461 | 55.8933 |
Translational Efficiency | 2.4089 | 2.1917 | 1.3593 | 1.246 | 1.3341 | 2.0702 | 1.2466 | 1.1387 | 1.0507 | 1.2335 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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