Standard name
Human Ortholog
Description Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.99 0.97 0.88 0.81 0.8 0.74 0.68 0.67 0.71 0.98 0.98 0.98 0.97 0.99 0.99 0.97 0.98 0.95 0.93 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.06 0.06 0.12 0.11 0.23 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.09 0.11 0.13 0.2 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 5 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 0 2 1 0 2 2 0 0 0 0 0 0 1 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 0 2 0 3 3 9 15 20 20 20 1 1 1 4 3 6 0 0 0 0 0 0
Cytoplasm 550 446 284 255 383 326 380 467 341 349 284 338 317 372 445 211 181 172 537 432 261 236 298 406
Endoplasmic Reticulum 1 0 2 1 2 8 21 8 12 15 17 8 6 7 6 3 6 3 0 1 3 1 2 7
Endosome 1 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 0
Mitochondria 1 0 1 0 2 18 28 38 56 55 98 81 0 0 0 1 1 1 0 0 0 1 2 2
Nucleus 0 1 0 1 2 3 10 18 13 8 11 15 3 0 4 2 0 0 1 1 1 1 1 3
Nuclear Periphery 0 0 0 0 0 0 1 0 4 2 3 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 0 11 12 22 44 63 60 104 37 33 0 1 3 3 3 0 6 0 0 3 4 8
Unique Cell Count 558 447 286 258 396 371 471 585 461 511 425 473 322 378 454 217 183 173 554 440 275 253 317 442
Labelled Cell Count 562 453 287 270 401 382 488 610 502 559 473 501 327 381 459 224 194 182 554 440 275 253 317 442


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.9 17.5 13.8 13.0 12.2 10.9 10.3 10.4 10.1 9.8 9.6 9.9 15.3 14.3 14.4 21.4 21.6 21.0 16.5 17.3 16.8
Std Deviation (1e-4) 2.3 2.6 2.3 2.1 1.9 1.9 2.0 2.0 2.0 2.0 2.0 2.2 2.2 2.1 2.0 4.6 5.2 5.0 2.4 2.7 3.0
Intensity Change (Log2) – – – -0.08 -0.18 -0.34 -0.42 -0.4 -0.45 -0.49 -0.53 -0.48 0.15 0.06 0.07 0.64 0.65 0.61 0.26 0.33 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.1 3.4 3.7 3.5 0 0 0 0 0 0
Cytoplasm -0.6 -2.3 -5.6 -7.6 -7.8 -9.1 -10.4 -10.6 -9.7 -1.0 -1.0 -1.4 -1.8 -0.5 0.2
Endoplasmic Reticulum 0 0 0 2.9 0 1.9 2.1 2.7 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 3.9 4.1 5.9 5.5 8.6 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 3.0 0 0 0 3.0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.5 3.0 4.2 5.3 5.8 6.4 8.2 5.1 4.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5265 11.6086 10.848 9.7686 10.2649 10.8992 11.8519 14.2765 13.2406 13.6771 13.1104 13.2121 9.7139 12.8538 10.9447 10.7472 9.8253 11.7417
Actin 0.0224 0.0005 0.0004 0.0118 0.0044 0.0004 0.0442 0.0004 0.0104 0.0002 0.0003 0.0035 0.0152 0.0001 0.0105 0.0014 0.011 0.0025
Bud 0.0002 0.0001 0.0001 0.0006 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0.0003 0.0001 0.0002 0.0002
Bud Neck 0.0008 0.0002 0.0003 0.0034 0.0008 0.0007 0.0045 0.0002 0.0003 0.0006 0.0004 0.0011 0.0006 0.0001 0.0006 0.0004 0.0005 0.0011
Bud Periphery 0.0001 0 0 0.0021 0.0007 0 0.0007 0 0 0 0 0 0.0004 0 0.0004 0 0.0003 0.0002
Bud Site 0.0011 0.0004 0.0001 0.0058 0.0004 0 0.002 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0008 0.0001 0.0004 0.0001
Cell Periphery 0.0001 0 0 0.0016 0.0001 0 0.0005 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.8983 0.9798 0.9784 0.8537 0.8854 0.9545 0.864 0.9874 0.9759 0.9732 0.983 0.9618 0.9069 0.9884 0.955 0.9708 0.7502 0.9516
Cytoplasmic Foci 0.0109 0.0014 0.0011 0.0078 0.0052 0.0014 0.0183 0.0007 0.0008 0.0011 0.001 0.0027 0.0063 0.0005 0.0071 0.0042 0.0077 0.0018
Eisosomes 0.0002 0 0 0.0009 0 0 0.0007 0 0 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0045 0.0007 0.0013 0.0092 0.0039 0.0035 0.0041 0.0008 0.0008 0.0005 0.001 0.0029 0.0074 0.0011 0.001 0.0036 0.0051 0.0024
Endosome 0.0101 0.0004 0.0004 0.015 0.0173 0.0025 0.0154 0.0004 0.0003 0.0002 0.0003 0.0031 0.0132 0.0003 0.004 0.0043 0.073 0.0031
Golgi 0.0017 0.0001 0 0.0019 0.0013 0.0001 0.0027 0 0.0001 0 0 0.0004 0.0036 0 0.0026 0.0005 0.04 0.0005
Lipid Particles 0.0018 0 0 0.0021 0.0008 0 0.0022 0 0 0 0 0.0003 0.0014 0 0.0025 0.0001 0.049 0.0002
Mitochondria 0.0007 0.0001 0.0001 0.0017 0.0011 0.0001 0.0009 0.0001 0.0001 0.0001 0.0001 0.0002 0.0056 0 0.001 0.0006 0.0229 0.0136
None 0.0055 0.0009 0.002 0.006 0.0029 0.0009 0.0189 0.0009 0.0011 0.0009 0.0014 0.0014 0.0066 0.0007 0.0014 0.0018 0.0009 0.0009
Nuclear Periphery 0.0097 0.0017 0.0014 0.0144 0.0109 0.0058 0.0037 0.0012 0.0009 0.0011 0.0017 0.0027 0.0081 0.0009 0.0008 0.0016 0.0046 0.0026
Nucleolus 0.0002 0 0 0.0007 0.0005 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Nucleus 0.0233 0.0129 0.0137 0.0551 0.0345 0.0285 0.0086 0.0072 0.0084 0.0214 0.01 0.0177 0.0185 0.0073 0.0076 0.0092 0.0235 0.0182
Peroxisomes 0.0012 0 0 0.0006 0.0005 0 0.0025 0 0 0 0 0.0001 0.0009 0 0.0033 0.0004 0.0047 0.0002
Punctate Nuclear 0.0048 0.0004 0.0004 0.0019 0.007 0.0006 0.0037 0.0003 0.0003 0.0004 0.0006 0.0014 0.0013 0.0002 0.0006 0.0005 0.0007 0.0005
Vacuole 0.002 0.0003 0.0003 0.003 0.018 0.0007 0.0013 0.0001 0.0001 0.0001 0.0001 0.0003 0.0014 0.0001 0.0003 0.0004 0.0025 0.0003
Vacuole Periphery 0.0005 0.0001 0 0.0008 0.0037 0.0002 0.0004 0 0 0 0 0.0001 0.0017 0 0.0001 0.0001 0.0023 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.006 60.1807 104.562 110.9325 62.8022 47.5908 73.8346 94.1576 97.2532 73.59
Translational Efficiency 2.3032 2.6263 1.8616 1.7782 2.3631 2.5089 2.4316 1.9305 1.8968 1.9458

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
909 1392 2411 115 2134 2471 2235 809 3043 3863 4646 924

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 959.93 1272.22 1659.19 1968.60 1252.30 1284.66 1774.62 1780.74 1164.96 1280.18 1714.72 1804.12
Standard Deviation 145.50 210.98 232.95 376.94 176.00 199.25 253.38 305.32 214.37 203.64 249.74 321.17
Intensity Change Log 2 — 0.406347 0.789478 1.036169 — 0.036806 0.502930 0.507897 — 0.208883 0.634309 0.761135

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000380 0.000205 0.000063 0.017295 0.000096 0.000160 0.000138 0.001413 0.000180 0.000176 0.000099 0.003390
Bud Neck 0.000920 0.000454 0.000411 0.017734 0.000371 0.000964 0.000992 0.002749 0.000535 0.000780 0.000691 0.004614
Bud Site 0.015161 0.002834 0.000587 0.066145 0.001532 0.004334 0.004390 0.035859 0.005603 0.003793 0.002417 0.039628
Cell Periphery 0.000179 0.000058 0.000036 0.001873 0.000036 0.000029 0.000083 0.000245 0.000079 0.000040 0.000058 0.000447
Cytoplasm 0.829742* 0.851048* 0.695787* 0.369862* 0.867407* 0.879125* 0.790312* 0.598129* 0.856156* 0.869007* 0.741259* 0.569719*
Cytoplasmic Foci 0.088866 0.010154 0.001561 0.051028 0.022743 0.019159 0.004205 0.026701 0.042495 0.015914 0.002833 0.029729
Eisosomes 0.000010 0.000003 0.000001 0.000101 0.000003 0.000003 0.000002 0.000022 0.000005 0.000003 0.000001 0.000032
Endoplasmic Reticulum 0.002282 0.000329 0.000050 0.005490 0.000561 0.000155 0.000227 0.000666 0.001075 0.000218 0.000135 0.001267
Endosome 0.001847 0.000515 0.000050 0.057731 0.000767 0.001120 0.001120 0.021077 0.001090 0.000902 0.000565 0.025639
Golgi 0.002124 0.000977 0.000061 0.077843 0.000367 0.001554 0.001182 0.025970 0.000892 0.001346 0.000600 0.032426
Lipid Particles 0.000573 0.000187 0.000111 0.024765 0.000277 0.000206 0.000265 0.000840 0.000365 0.000199 0.000185 0.003818
Mitochondria 0.000349 0.000581 0.000142 0.013137 0.000103 0.000464 0.000243 0.012715 0.000176 0.000506 0.000191 0.012768
Mitotic Spindle 0.002617 0.001330 0.003194 0.158184* 0.002794 0.005666 0.010699 0.098920 0.002741 0.004103 0.006804 0.106296*
None 0.001788 0.014069 0.013827 0.005974 0.005476 0.008223 0.009616 0.008080 0.004374 0.010329 0.011802 0.007818
Nuclear Periphery 0.000069 0.000054 0.000130 0.000326 0.000085 0.000064 0.000154 0.000281 0.000080 0.000060 0.000141 0.000286
Nuclear Periphery Foci 0.002282 0.002263 0.000374 0.004451 0.002398 0.001337 0.000688 0.001746 0.002364 0.001671 0.000525 0.002082
Nucleolus 0.000357 0.000240 0.000075 0.000546 0.000091 0.000130 0.000101 0.000117 0.000171 0.000170 0.000087 0.000171
Nucleus 0.036113 0.104500 0.278917* 0.039543 0.079734 0.063593 0.160124* 0.102638 0.066704 0.078334 0.221770* 0.094785
Peroxisomes 0.000368 0.000108 0.000014 0.007566 0.000066 0.000361 0.000225 0.000416 0.000156 0.000270 0.000116 0.001306
Vacuole 0.012857 0.009829 0.004589 0.068243 0.014954 0.013068 0.013564 0.042500 0.014328 0.011901 0.008906 0.045704
Vacuole Periphery 0.001116 0.000264 0.000020 0.012164 0.000139 0.000285 0.001670 0.018916 0.000430 0.000278 0.000814 0.018076

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.38 6.84 -1.52 -1.59 -1.65 -2.02 -2.58 -4.03 -2.91 -3.35 -0.03 3.34 -2.18 -2.17 -2.39
Bud Neck 3.06 1.04 -3.65 -3.90 -3.73 -2.53 -9.79 -8.25 -3.85 -4.84 -1.68 -6.46 -7.80 -6.16 -6.44
Bud Site 7.75 9.63 -0.44 -2.41 -2.73 -4.28 -3.42 -4.93 -3.39 -4.12 3.04 7.39 -3.23 -4.28 -5.36
Cell Periphery 7.01 8.05 -1.47 -1.79 -1.79 2.60 -5.05 -11.73 -12.60 -6.38 6.61 1.51 -3.21 -4.01 -3.42
Cytoplasm -1.76 23.77 13.83 14.44 8.17 -2.09 26.54 26.36 27.13 12.73 -2.21 40.59 28.19 29.34 11.45
Cytoplasmic Foci 17.59 19.98 13.45 -2.52 -5.69 2.08 16.30 5.45 3.73 -8.69 14.86 24.45 14.51 1.39 -11.16
Eisosomes 8.03 10.53 -2.10 -3.19 -3.42 -0.21 0.66 -6.49 -6.54 -6.84 5.87 9.19 -4.88 -6.78 -7.61
Endoplasmic Reticulum 4.46 5.01 2.91 -1.36 -2.12 3.14 3.43 3.16 0.21 -0.96 5.36 6.10 5.18 -0.15 -2.52
Endosome 5.40 8.30 -1.44 -1.88 -2.00 -1.04 2.12 -1.94 -1.58 -2.52 1.38 6.23 -2.17 -2.50 -3.34
Golgi 3.31 10.63 -0.92 -1.13 -1.25 -2.65 0.16 -2.13 -1.03 -2.14 -1.02 5.97 -1.93 -1.64 -2.52
Lipid Particles 4.88 3.46 -3.50 -3.65 -3.60 1.98 -7.04 -7.91 -8.23 -6.27 4.77 -2.80 -6.87 -7.30 -6.59
Mitochondria -0.38 2.11 -1.02 -0.82 -1.18 -2.17 -2.09 -1.88 -1.31 -1.77 -1.61 0.01 -1.99 -1.42 -1.99
Mitotic Spindle 1.26 -1.97 -3.14 -3.24 -2.99 -1.77 -3.69 -4.48 -3.85 -3.12 -1.09 -3.87 -5.45 -5.18 -4.52
None -10.54 -28.29* -3.12 3.75 4.16 -3.75 -6.73 0.04 4.03 7.56 -9.41 -17.56 -2.50 6.91 12.50
Nuclear Periphery 0.13 -9.90 -6.23 -6.31 -4.72 1.66 -18.20 -18.83 -17.98 -7.10 1.52 -21.37 -17.58 -18.06 -9.17
Nuclear Periphery Foci -0.54 7.07 -1.98 -1.74 -4.62 4.95 8.53 1.46 -3.96 -8.18 3.56 11.46 0.40 -3.03 -10.40
Nucleolus 0.52 0.97 0.15 -0.90 -2.93 -2.08 -8.05 -4.95 0.60 2.70 0.07 0.62 0.31 0.53 -2.16
Nucleus -21.27 -61.33 -4.04 2.14 17.98 6.81 -27.71 -4.39 -8.02 14.97 -6.71 -58.45 -7.44 -4.32 30.10
Peroxisomes 6.42 10.17 -1.24 -1.84 -2.03 -3.67 -0.24 -2.28 2.95 -0.96 -2.01 5.05 -0.97 0.58 -2.75
Vacuole -1.65 -3.66 -11.53 -11.43 -11.34 -1.51 -15.56 -24.48 -24.22 -19.65 -1.89 -13.62 -26.67 -26.42 -24.21
Vacuole Periphery 4.28 5.73 -0.29 -1.50 -1.78 -1.88 -1.03 -2.07 -1.91 -1.67 2.38 1.79 -1.83 -2.11 -2.15
Standard name
Human Ortholog
Description Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ypt31

Ypt31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypt31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available