Standard name | |
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Human Ortholog | |
Description | Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | 2 | 0 | 6 | 7 | 0 | 0 | 0 |
Bud | – | 2 | 5 | 2 | 2 | 2 | 5 | 8 | – | 6 | 3 | 8 | – | – | – | – | – | 2 | 0 | 3 | 3 | 0 | 0 | 0 |
Bud Neck | – | 3 | 5 | 2 | 0 | 3 | 0 | 4 | – | 3 | 2 | 1 | – | – | – | – | – | 3 | 0 | 3 | 2 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | – | 2 | 0 | 3 | – | – | – | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 0 | – | – | – | – | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 |
Cytoplasm | – | 335 | 214 | 122 | 175 | 205 | 247 | 281 | – | 108 | 120 | 171 | – | – | – | – | – | 90 | 36 | 270 | 126 | 4 | 2 | 1 |
Endoplasmic Reticulum | – | 6 | 0 | 1 | 0 | 0 | 0 | 3 | – | 0 | 0 | 1 | – | – | – | – | – | 19 | 0 | 1 | 6 | 0 | 0 | 0 |
Endosome | – | 2 | 3 | 0 | 2 | 0 | 1 | 1 | – | 0 | 0 | 0 | – | – | – | – | – | 21 | 2 | 18 | 14 | 0 | 0 | 0 |
Golgi | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 1 | 2 | 2 | 0 | 0 | 0 |
Mitochondria | – | 35 | 10 | 17 | 21 | 92 | 180 | 202 | – | 139 | 159 | 242 | – | – | – | – | – | 13 | 10 | 46 | 49 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 1 | 0 | – | – | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | – | – | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | – | – | – | – | – | 3 | 1 | 3 | 4 | 0 | 0 | 0 |
SpindlePole | – | 2 | 6 | 2 | 0 | 3 | 4 | 7 | – | 5 | 2 | 9 | – | – | – | – | – | 38 | 1 | 7 | 6 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 2 | 4 | 0 | 1 | 1 | 2 | 2 | – | 4 | 4 | 2 | – | – | – | – | – | 4 | 1 | 10 | 7 | 0 | 0 | 0 |
Unique Cell Count | 367 | 234 | 133 | 186 | 248 | 338 | 409 | 209 | 242 | 331 | 163 | 59 | 385 | 242 | 6 | 5 | 4 | |||||||
Labelled Cell Count | 387 | 251 | 147 | 202 | 309 | 440 | 513 | 268 | 295 | 438 | 203 | 59 | 385 | 242 | 6 | 5 | 4 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 3.7 | 3.6 | 3.1 | 3.2 | 2.6 | 2.4 | 2.8 | – | 2.4 | 2.5 | 2.5 | – | – | – | 4.3 | 4.4 | 4.9 | – | – | – |
Std Deviation (1e-4) | – | 0.6 | 1.3 | 0.7 | 1.2 | 1.2 | 0.6 | 0.7 | – | 0.4 | 0.8 | 0.5 | – | – | – | 1.2 | 0.9 | 1.5 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.24 | -0.19 | -0.46 | -0.62 | -0.39 | – | -0.62 | -0.56 | -0.56 | – | – | – | 0.24 | 0.29 | 0.44 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7575 | 0.46 | 0.2405 | 0.4699 | 0.5954 | 1.0698 | 4.0213 | 2.5339 | 2.4017 | 2.6903 | 4.0463 | 2.447 | 2.323 | 1.0736 | 2.1031 | 2.1487 | 3.9528 | 1.8858 |
Actin | 0.0495 | 0.0056 | 0.0089 | 0.006 | 0.0072 | 0.002 | 0.0006 | 0.0001 | 0.0047 | 0.0001 | 0.0255 | 0.0006 | 0.0021 | 0.0021 | 0.0479 | 0.0244 | 0.0016 | 0.0292 |
Bud | 0.0023 | 0.0054 | 0.0028 | 0.0004 | 0.0021 | 0.0016 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0007 | 0 | 0.0101 | 0.0004 | 0.0001 | 0.0012 | 0.0001 | 0.0004 |
Bud Neck | 0.003 | 0.0013 | 0.0008 | 0.0003 | 0.0003 | 0.002 | 0.0014 | 0.0012 | 0.0102 | 0 | 0.001 | 0.0005 | 0.0043 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0016 |
Bud Periphery | 0.0096 | 0.0089 | 0.014 | 0.0012 | 0.0038 | 0.0018 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0435 | 0.0004 | 0.0002 | 0.0016 | 0.0001 | 0.0005 |
Bud Site | 0.0029 | 0.03 | 0.0019 | 0.002 | 0.0017 | 0.0004 | 0.0062 | 0.0006 | 0.0182 | 0 | 0.0027 | 0.0001 | 0.0322 | 0.0022 | 0.0011 | 0.0017 | 0.0001 | 0.0004 |
Cell Periphery | 0.0007 | 0.0007 | 0.0032 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0147 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 |
Cytoplasm | 0.0542 | 0.0518 | 0.0676 | 0.1019 | 0.1181 | 0.0333 | 0.2436 | 0.0603 | 0.1412 | 0.0498 | 0.0588 | 0.1229 | 0.0153 | 0.1311 | 0.0511 | 0.1076 | 0.1716 | 0.0911 |
Cytoplasmic Foci | 0.0763 | 0.0589 | 0.0877 | 0.1728 | 0.0792 | 0.0661 | 0.0892 | 0.103 | 0.099 | 0.0105 | 0.239 | 0.1083 | 0.0669 | 0.2819 | 0.0028 | 0.1007 | 0.1977 | 0.0567 |
Eisosomes | 0.0007 | 0.0004 | 0.0041 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0027 | 0.0003 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0053 | 0.0036 | 0.0054 | 0.0119 | 0.0023 | 0.0008 | 0.0004 | 0.002 | 0.0087 | 0.0006 | 0.0046 | 0.0031 | 0.0017 | 0.0126 | 0.0034 | 0.0321 | 0.0085 | 0.0027 |
Endosome | 0.0487 | 0.0607 | 0.0531 | 0.1428 | 0.0638 | 0.0167 | 0.0514 | 0.0684 | 0.0728 | 0.0047 | 0.1272 | 0.0456 | 0.0697 | 0.114 | 0.0018 | 0.1704 | 0.0437 | 0.0445 |
Golgi | 0.0102 | 0.0072 | 0.01 | 0.0072 | 0.0208 | 0.0048 | 0.0122 | 0.0046 | 0.0034 | 0.0022 | 0.0631 | 0.0039 | 0.0063 | 0.0058 | 0.0015 | 0.0109 | 0.0007 | 0.0244 |
Lipid Particles | 0.168 | 0.0584 | 0.0512 | 0.0082 | 0.0222 | 0.0549 | 0.1956 | 0.0462 | 0.0724 | 0.1048 | 0.0541 | 0.0723 | 0.0463 | 0.0514 | 0.0023 | 0.0645 | 0.017 | 0.0559 |
Mitochondria | 0.01 | 0.0064 | 0.0133 | 0.0147 | 0.0162 | 0.0036 | 0.0075 | 0.0005 | 0.0004 | 0.0003 | 0.0261 | 0.0186 | 0.0066 | 0.001 | 0.0023 | 0.0025 | 0.0012 | 0.0051 |
None | 0.4258 | 0.6335 | 0.6436 | 0.4705 | 0.4917 | 0.6938 | 0.2597 | 0.6873 | 0.5506 | 0.8184 | 0.2383 | 0.5867 | 0.5153 | 0.3702 | 0.8741 | 0.4242 | 0.3404 | 0.6574 |
Nuclear Periphery | 0.0167 | 0.0019 | 0.002 | 0.018 | 0.0007 | 0.006 | 0.0006 | 0.0007 | 0.0025 | 0.0002 | 0.0053 | 0.0009 | 0.0024 | 0.005 | 0.0025 | 0.0172 | 0.1588 | 0.0011 |
Nucleolus | 0.0063 | 0.011 | 0.002 | 0.0008 | 0.0002 | 0.0028 | 0.007 | 0.0007 | 0.0003 | 0.0001 | 0.0012 | 0.0003 | 0.0071 | 0.0006 | 0.0001 | 0.0004 | 0.0005 | 0.0002 |
Nucleus | 0.0452 | 0.0049 | 0.0011 | 0.0105 | 0.0004 | 0.0236 | 0.0037 | 0.0005 | 0.001 | 0.0001 | 0.0037 | 0.0004 | 0.0076 | 0.0011 | 0.0005 | 0.0049 | 0.0445 | 0.0009 |
Peroxisomes | 0.0274 | 0.0257 | 0.0144 | 0.0069 | 0.1631 | 0.0592 | 0.092 | 0.0077 | 0.0041 | 0.007 | 0.0799 | 0.02 | 0.0997 | 0.0079 | 0.0035 | 0.0034 | 0.001 | 0.0239 |
Punctate Nuclear | 0.0281 | 0.0174 | 0.0047 | 0.0151 | 0.0018 | 0.0242 | 0.0103 | 0.0018 | 0.0045 | 0.0003 | 0.0571 | 0.0094 | 0.0011 | 0.0049 | 0.0004 | 0.0039 | 0.0057 | 0.0016 |
Vacuole | 0.0068 | 0.0057 | 0.0062 | 0.0055 | 0.0031 | 0.0019 | 0.0145 | 0.0131 | 0.0045 | 0.0005 | 0.0078 | 0.0043 | 0.0395 | 0.0061 | 0.0007 | 0.0239 | 0.0028 | 0.0015 |
Vacuole Periphery | 0.0022 | 0.0007 | 0.0019 | 0.0015 | 0.001 | 0.0003 | 0.0018 | 0.0007 | 0.0004 | 0.0001 | 0.0021 | 0.0013 | 0.0073 | 0.0007 | 0.0002 | 0.0038 | 0.004 | 0.0006 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.8765 | 32.8792 | 18.9356 | 33.0191 | 42.7048 | 36.2111 | 33.7403 | 32.8191 | 30.5916 | 49.2648 |
Translational Efficiency | 0.8684 | 0.8177 | 1.0558 | 0.6227 | 0.6858 | 1.1387 | 0.6591 | 0.5502 | 0.7083 | 0.565 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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