Standard name
Human Ortholog
Description Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0.24
Bud – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
Cytoplasm – 0.91 0.91 0.92 0.94 0.83 0.73 0.69 – 0.52 0.5 0.52 – – – – – 0.55 0.62 0.7 0.52 0.68 0.48 0.28
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0.12 0 0 0 0.09 0.08 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0.13 0.05 0.05 0.06 0 0.06 0.06
Golgi – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0.14 0.15
Mitochondria – 0.1 0 0.13 0.11 0.37 0.53 0.49 – 0.67 0.66 0.73 – – – – – 0.08 0.18 0.12 0.2 0.16 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0.23 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 – 0 0 0 – – – – – 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 1 0 0 0 0 0 – 0 0 0 – – – – – 2 0 6 7 0 0 0
Bud – 2 5 2 2 2 5 8 – 6 3 8 – – – – – 2 0 3 3 0 0 0
Bud Neck – 3 5 2 0 3 0 4 – 3 2 1 – – – – – 3 0 3 2 0 0 0
Bud Site – 0 0 0 0 1 1 2 – 2 0 3 – – – – – 0 – – – – – –
Cell Periphery – 0 1 0 0 0 0 0 – 1 3 0 – – – – – 1 0 1 2 0 0 0
Cytoplasm – 335 214 122 175 205 247 281 – 108 120 171 – – – – – 90 36 270 126 4 2 1
Endoplasmic Reticulum – 6 0 1 0 0 0 3 – 0 0 1 – – – – – 19 0 1 6 0 0 0
Endosome – 2 3 0 2 0 1 1 – 0 0 0 – – – – – 21 2 18 14 0 0 0
Golgi – 0 2 0 0 0 0 0 – 0 0 0 – – – – – 0 1 2 2 0 0 0
Mitochondria – 35 10 17 21 92 180 202 – 139 159 242 – – – – – 13 10 46 49 0 0 0
Nucleus – 0 0 0 1 1 0 1 – 0 1 0 – – – – – 3 0 0 0 0 0 0
Nuclear Periphery – 0 0 1 0 0 0 1 – 0 1 1 – – – – – 1 0 0 0 0 0 0
Nucleolus – 0 0 0 0 1 0 0 – 0 0 0 – – – – – 3 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 1 – 0 0 0 – – – – – 3 1 3 4 0 0 0
SpindlePole – 2 6 2 0 3 4 7 – 5 2 9 – – – – – 38 1 7 6 0 0 0
Vac/Vac Membrane – 2 4 0 1 1 2 2 – 4 4 2 – – – – – 4 1 10 7 0 0 0
Unique Cell Count 367 234 133 186 248 338 409 209 242 331 163 59 385 242 6 5 4
Labelled Cell Count 387 251 147 202 309 440 513 268 295 438 203 59 385 242 6 5 4


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 3.7 3.6 3.1 3.2 2.6 2.4 2.8 – 2.4 2.5 2.5 – – – 4.3 4.4 4.9 – – –
Std Deviation (1e-4) – 0.6 1.3 0.7 1.2 1.2 0.6 0.7 – 0.4 0.8 0.5 – – – 1.2 0.9 1.5 – – –
Intensity Change (Log2) – – – -0.24 -0.19 -0.46 -0.62 -0.39 – -0.62 -0.56 -0.56 – – – 0.24 0.29 0.44 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7575 0.46 0.2405 0.4699 0.5954 1.0698 4.0213 2.5339 2.4017 2.6903 4.0463 2.447 2.323 1.0736 2.1031 2.1487 3.9528 1.8858
Actin 0.0495 0.0056 0.0089 0.006 0.0072 0.002 0.0006 0.0001 0.0047 0.0001 0.0255 0.0006 0.0021 0.0021 0.0479 0.0244 0.0016 0.0292
Bud 0.0023 0.0054 0.0028 0.0004 0.0021 0.0016 0.0006 0.0001 0.0002 0 0.0007 0 0.0101 0.0004 0.0001 0.0012 0.0001 0.0004
Bud Neck 0.003 0.0013 0.0008 0.0003 0.0003 0.002 0.0014 0.0012 0.0102 0 0.001 0.0005 0.0043 0.0003 0.0005 0.0002 0.0001 0.0016
Bud Periphery 0.0096 0.0089 0.014 0.0012 0.0038 0.0018 0.0009 0.0001 0.0001 0 0.0012 0.0001 0.0435 0.0004 0.0002 0.0016 0.0001 0.0005
Bud Site 0.0029 0.03 0.0019 0.002 0.0017 0.0004 0.0062 0.0006 0.0182 0 0.0027 0.0001 0.0322 0.0022 0.0011 0.0017 0.0001 0.0004
Cell Periphery 0.0007 0.0007 0.0032 0.0004 0.0001 0.0001 0.0007 0.0001 0.0004 0 0.0002 0.0003 0.0147 0.0001 0.0002 0.0003 0 0.0002
Cytoplasm 0.0542 0.0518 0.0676 0.1019 0.1181 0.0333 0.2436 0.0603 0.1412 0.0498 0.0588 0.1229 0.0153 0.1311 0.0511 0.1076 0.1716 0.0911
Cytoplasmic Foci 0.0763 0.0589 0.0877 0.1728 0.0792 0.0661 0.0892 0.103 0.099 0.0105 0.239 0.1083 0.0669 0.2819 0.0028 0.1007 0.1977 0.0567
Eisosomes 0.0007 0.0004 0.0041 0.0013 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0004 0.0003 0.0004 0.0002 0.0027 0.0003 0 0.0002
Endoplasmic Reticulum 0.0053 0.0036 0.0054 0.0119 0.0023 0.0008 0.0004 0.002 0.0087 0.0006 0.0046 0.0031 0.0017 0.0126 0.0034 0.0321 0.0085 0.0027
Endosome 0.0487 0.0607 0.0531 0.1428 0.0638 0.0167 0.0514 0.0684 0.0728 0.0047 0.1272 0.0456 0.0697 0.114 0.0018 0.1704 0.0437 0.0445
Golgi 0.0102 0.0072 0.01 0.0072 0.0208 0.0048 0.0122 0.0046 0.0034 0.0022 0.0631 0.0039 0.0063 0.0058 0.0015 0.0109 0.0007 0.0244
Lipid Particles 0.168 0.0584 0.0512 0.0082 0.0222 0.0549 0.1956 0.0462 0.0724 0.1048 0.0541 0.0723 0.0463 0.0514 0.0023 0.0645 0.017 0.0559
Mitochondria 0.01 0.0064 0.0133 0.0147 0.0162 0.0036 0.0075 0.0005 0.0004 0.0003 0.0261 0.0186 0.0066 0.001 0.0023 0.0025 0.0012 0.0051
None 0.4258 0.6335 0.6436 0.4705 0.4917 0.6938 0.2597 0.6873 0.5506 0.8184 0.2383 0.5867 0.5153 0.3702 0.8741 0.4242 0.3404 0.6574
Nuclear Periphery 0.0167 0.0019 0.002 0.018 0.0007 0.006 0.0006 0.0007 0.0025 0.0002 0.0053 0.0009 0.0024 0.005 0.0025 0.0172 0.1588 0.0011
Nucleolus 0.0063 0.011 0.002 0.0008 0.0002 0.0028 0.007 0.0007 0.0003 0.0001 0.0012 0.0003 0.0071 0.0006 0.0001 0.0004 0.0005 0.0002
Nucleus 0.0452 0.0049 0.0011 0.0105 0.0004 0.0236 0.0037 0.0005 0.001 0.0001 0.0037 0.0004 0.0076 0.0011 0.0005 0.0049 0.0445 0.0009
Peroxisomes 0.0274 0.0257 0.0144 0.0069 0.1631 0.0592 0.092 0.0077 0.0041 0.007 0.0799 0.02 0.0997 0.0079 0.0035 0.0034 0.001 0.0239
Punctate Nuclear 0.0281 0.0174 0.0047 0.0151 0.0018 0.0242 0.0103 0.0018 0.0045 0.0003 0.0571 0.0094 0.0011 0.0049 0.0004 0.0039 0.0057 0.0016
Vacuole 0.0068 0.0057 0.0062 0.0055 0.0031 0.0019 0.0145 0.0131 0.0045 0.0005 0.0078 0.0043 0.0395 0.0061 0.0007 0.0239 0.0028 0.0015
Vacuole Periphery 0.0022 0.0007 0.0019 0.0015 0.001 0.0003 0.0018 0.0007 0.0004 0.0001 0.0021 0.0013 0.0073 0.0007 0.0002 0.0038 0.004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.8765 32.8792 18.9356 33.0191 42.7048 36.2111 33.7403 32.8191 30.5916 49.2648
Translational Efficiency 0.8684 0.8177 1.0558 0.6227 0.6858 1.1387 0.6591 0.5502 0.7083 0.565

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
163 234 72 1432 181 1147 1835 1395 344 1381 1907 2827

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 860.31 688.37 995.34 801.08 601.78 711.44 827.03 829.86 724.28 707.53 833.38 815.28
Standard Deviation 167.59 87.93 123.46 135.64 99.63 90.02 112.56 125.10 187.60 90.09 117.45 131.34
Intensity Change Log 2 — -0.321672 0.210333 -0.102910 — 0.241506 0.458704 0.463632 — -0.062801 0.317784 0.157672

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001480 0.001457 0.001005 0.012137 0.000144 0.000663 0.011524 0.014780 0.000777 0.000797 0.011126 0.013441
Bud Neck 0.009291 0.058256 0.002331 0.010177 0.029725 0.050723 0.010137 0.006993 0.020043 0.051999 0.009842 0.008606
Bud Site 0.038040 0.025199 0.006337 0.030722 0.004068 0.019382 0.018275 0.025125 0.020165 0.020368 0.017824 0.027960
Cell Periphery 0.000730 0.000179 0.000444 0.000142 0.000205 0.000322 0.000141 0.000147 0.000454 0.000298 0.000153 0.000145
Cytoplasm 0.183196* 0.246463* 0.268100* 0.082649 0.345274* 0.295432* 0.131312* 0.098728 0.268475* 0.287135* 0.136477* 0.090583
Cytoplasmic Foci 0.126677 0.347831* 0.114442 0.226985* 0.276079* 0.355581* 0.186458* 0.258149* 0.205287* 0.354268* 0.183739* 0.242363*
Eisosomes 0.000137 0.000217 0.000180 0.000081 0.000255 0.000309 0.000081 0.000106 0.000199 0.000293 0.000085 0.000093
Endoplasmic Reticulum 0.002754 0.000753 0.014615 0.001558 0.000826 0.000653 0.002362 0.001827 0.001739 0.000670 0.002825 0.001690
Endosome 0.060407 0.043506 0.074695 0.176419 0.026709 0.037552 0.104119 0.179743 0.042676 0.038561 0.103009 0.178060
Golgi 0.021266 0.007725 0.001003 0.050647 0.002101 0.016581 0.019088 0.055185 0.011182 0.015080 0.018406 0.052886
Lipid Particles 0.013673 0.016244 0.050333 0.055783 0.024308 0.018313 0.079300 0.064547 0.019269 0.017962 0.078207 0.060108
Mitochondria 0.004628 0.020125 0.000234 0.013390 0.010805 0.033779 0.004917 0.008661 0.007878 0.031465 0.004740 0.011057
Mitotic Spindle 0.014173 0.008508 0.002360 0.158820* 0.000190 0.011103 0.186672* 0.092010 0.006815 0.010663 0.179713* 0.125852*
None 0.000563 0.005962 0.001204 0.001411 0.004065 0.004812 0.002201 0.002116 0.002406 0.005007 0.002164 0.001759
Nuclear Periphery 0.000942 0.000431 0.007907 0.001173 0.001016 0.000260 0.002454 0.000690 0.000981 0.000289 0.002660 0.000935
Nuclear Periphery Foci 0.001980 0.001432 0.102086 0.009228 0.000692 0.000263 0.012245 0.008601 0.001302 0.000461 0.015637 0.008918
Nucleolus 0.005193 0.003335 0.001481 0.002084 0.002931 0.001871 0.003881 0.001638 0.004002 0.002119 0.003791 0.001864
Nucleus 0.118196 0.087520 0.098599 0.019260 0.185892* 0.059239 0.082550 0.025946 0.153815* 0.064031 0.083156 0.022559
Peroxisomes 0.037047 0.062607 0.004264 0.084855 0.013532 0.044032 0.096071 0.103909* 0.024674 0.047180 0.092605 0.094257
Vacuole 0.339954* 0.060358 0.247720* 0.058087 0.069006 0.045540 0.045355 0.047941 0.197391 0.048050 0.052996 0.053080
Vacuole Periphery 0.019672 0.001892 0.000659 0.004390 0.002178 0.003591 0.000856 0.003160 0.010467 0.003303 0.000849 0.003783

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.04 0.81 -6.36 -6.33 -6.99 -5.10 -7.40 -7.32 -7.02 -1.80 -0.21 -6.79 -9.76 -9.88 -1.83
Bud Neck -5.13 2.07 -0.78 5.13 -8.66 -3.53 3.13 4.05 13.56 4.64 -7.33 2.29 3.04 13.83 2.35
Bud Site 0.85 2.72 0.36 -1.10 -8.64 -6.98 -7.61 -8.44 -1.89 -2.69 -0.45 0.14 -1.94 -3.04 -4.75
Cell Periphery 1.36 0.50 1.48 1.64 6.02 -1.55 2.03 2.08 2.40 0.08 0.51 1.58 1.69 2.54 1.12
Cytoplasm -2.54 -1.95 4.18 8.28 4.96 2.07 8.36 10.39 17.98 5.36 -1.16 6.73 10.38 21.20 7.39
Cytoplasmic Foci -11.53 0.60 -6.82 8.36 -6.88 -4.23 6.71 1.72 11.91 -12.29 -11.60 3.35 -2.14 16.81 -11.77
Eisosomes -3.67 -1.94 3.75 7.72 5.65 -1.04 3.30 2.88 14.29 -3.38 -3.17 3.91 3.71 16.86 -1.22
Endoplasmic Reticulum 2.83 -3.96 1.14 -5.27 4.42 0.74 -7.63 -2.58 -3.48 3.29 2.98 -4.62 -0.50 -5.91 5.62
Endosome 0.60 -1.26 -10.20 -14.34 -5.66 -1.77 -8.77 -17.40 -21.15 -12.32 -0.22 -7.68 -18.96 -27.29 -14.79
Golgi 1.00 1.89 -3.42 -13.21 -17.79 -8.61 -8.94 -16.86 -10.53 -12.18 -1.78 -1.40 -9.17 -14.07 -15.77
Lipid Particles -0.99 -2.12 -8.31 -7.29 -0.68 1.04 -6.43 -5.43 -10.82 1.26 0.15 -10.03 -8.85 -13.09 2.62
Mitochondria -3.36 2.11 -3.88 1.65 -8.17 -4.47 1.38 0.60 7.77 -2.43 -6.80 1.11 -1.07 7.55 -4.94
Mitotic Spindle 0.98 1.75 -12.31 -17.44 -21.45 -5.94 -25.85* -16.51 -13.77 9.42 -0.50 -20.69 -18.87 -23.33 5.62
None -1.34 -1.10 -2.97 1.05 -1.87 -0.38 1.42 1.51 1.86 0.39 -1.80 0.07 0.74 2.36 1.18
Nuclear Periphery 3.88 -1.70 -2.92 -6.36 1.50 3.03 -6.84 0.33 -6.98 8.56 5.21 -7.84 -1.61 -10.16 7.37
Nuclear Periphery Foci 0.98 -4.78 -5.99 -6.01 4.35 1.27 -10.43 -6.31 -6.92 2.88 3.25 -10.78 -8.74 -10.14 4.42
Nucleolus 0.23 1.06 0.20 -0.10 -2.21 1.34 -2.04 0.89 -0.73 3.94 1.17 -1.30 0.52 -1.38 3.07
Nucleus 1.82 0.94 5.38 6.15 4.06 7.27 4.07 8.70 5.96 13.52 7.35 3.46 9.97 8.91 15.08
Peroxisomes -2.82 2.89 -4.87 -1.81 -15.83 -8.22 -13.61 -14.98 -9.54 -3.12 -5.21 -9.39 -12.10 -9.69 -2.34
Vacuole 11.74 2.24 10.77 -3.75 7.21 2.46 -0.25 0.26 -5.09 1.09 10.54 8.32 8.43 -8.47 0.25
Vacuole Periphery 1.97 2.18 1.65 -2.45 -4.32 -1.95 2.56 -0.76 0.87 -2.82 1.50 2.38 1.49 -0.01 -4.90
Standard name
Human Ortholog
Description Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Fat1

Fat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available