Standard name
Human Ortholog
Description RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.1 0.08 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0 0.07 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.94 0.93 0.93 0.94 0.84 0.77 0.73 0.62 0.63 0.66 0.68 0.92 0.89 0.8 0.78 0.8 0.75 0.9 0.91 0.9 0.74 0.6 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.1 0.07 0.07 0.15 0.26 0.3 0.46 0.42 0.46 0.42 0.16 0.16 0.23 0.1 0.16 0.16 0.05 0 0 0.16 0.23 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.07 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 3 3 2 3 5 1 1 0 0 1 0 3 5 1 0 3 3
Bud Neck 0 0 1 0 2 1 3 6 4 5 2 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 4 5 8 5 10 9 0 1 2 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 4 7 11 20 6 12 6 10 10 8 11 23 42 10 7 6 0 0 2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 3 0 0 1 0 1 1
Endosome 0 2 1 0 1 0 0 1 0 0 1 1 1 0 1 0 3 0 2 3 1 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0 1 2
Mitochondria 0 8 0 4 4 16 17 19 31 42 19 3 2 2 6 2 2 1 0 1 0 4 11
Nucleus 257 245 302 277 363 236 323 276 277 360 353 334 378 346 97 94 100 248 250 327 237 254 248
Nuclear Periphery 0 0 0 3 3 4 7 3 6 9 2 2 2 1 1 2 1 0 0 0 0 0 6
Nucleolus 37 25 24 20 64 80 132 206 182 250 220 59 67 100 13 19 22 13 6 13 50 97 102
Peroxisomes 0 1 1 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 0 0 1 0 2 0 2 1 3 1 2 4 0 1 1 3 1 2 1 0 4
Vac/Vac Membrane 3 0 2 1 1 1 1 2 3 2 6 0 4 1 6 2 7 2 4 5 24 51 64
Unique Cell Count 272 263 324 295 431 307 440 444 437 546 522 364 425 435 125 117 134 275 276 366 321 424 455
Labelled Cell Count 298 287 338 316 459 349 505 530 522 692 628 412 481 502 135 130 146 275 276 366 321 424 455


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.9 15.3 12.9 12.4 13.0 10.1 10.7 10.8 11.0 10.1 11.4 13.8 13.6 12.9 15.3 17.7 14.9 13.0 13.7 13.8
Std Deviation (1e-4) 6.4 5.7 3.8 6.2 4.6 3.9 3.9 3.6 4.0 3.9 4.3 4.6 5.1 4.7 8.5 7.4 6.0 4.5 4.8 4.0
Intensity Change (Log2) -0.06 0.01 -0.36 -0.27 -0.26 -0.23 -0.36 -0.18 0.09 0.07 -0.01 0.24 0.45 0.21 0.01 0.08 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.8 -0.2 0.5 -0.9 0.1 -0.3 -0.7 0.7 2.2 4.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 3.6 3.8 4.9 5.1 3.5 0 0 0 0 0 0
Nucleus 0.3 -3.8 -5.8 -7.0 -9.8 -9.5 -9.1 -8.7 -0.7 -2.0 -5.3 -4.7 -3.9 -5.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 3.2 6.3 7.7 11.7 10.5 11.8 10.8 3.5 3.5 5.7 1.0 2.8 2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.4111 13.5445 13.4265 13.7997 12.3975 13.5174 14.1355 12.2336 11.5954 13.1531 9.1041 11.2537 15.6601 12.9366 11.6319 12.2175 10.4317 11.7883
Actin 0.0187 0 0.0059 0 0.0048 0 0.0053 0 0.0059 0.0004 0.0253 0.0016 0.0151 0 0.0008 0 0.0057 0.0001
Bud 0.0006 0 0.0001 0 0.0002 0 0.0002 0 0.0007 0.0001 0.0021 0.0002 0.0003 0 0.0002 0 0.0005 0.0001
Bud Neck 0.0005 0 0.0002 0 0.0012 0.0004 0.0002 0 0.0001 0 0.0007 0.0003 0.0004 0 0.0002 0 0.0002 0.0003
Bud Periphery 0.0007 0 0.0001 0 0.0004 0.0001 0.0003 0 0.0012 0.0002 0.0026 0.0005 0.0004 0 0.0001 0 0.0008 0.0001
Bud Site 0.0009 0.0003 0.0004 0 0.0005 0.0001 0.0005 0.0001 0.0016 0.0003 0.0033 0.0003 0.0011 0 0.0026 0 0.001 0.0001
Cell Periphery 0.0004 0 0.0001 0 0.0003 0.0001 0.0002 0 0.0003 0.0001 0.0019 0.0001 0.0001 0 0.0001 0 0.0009 0.0001
Cytoplasm 0.0025 0 0.0007 0 0.0009 0 0.001 0 0.0009 0.0001 0.0032 0.0009 0.0045 0 0.0008 0 0.0133 0.0005
Cytoplasmic Foci 0.0099 0 0.0022 0 0.0035 0 0.0088 0.0001 0.0064 0.0026 0.0067 0.0031 0.0066 0 0.0067 0 0.0465 0.0019
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0013 0 0.0003 0 0.0011 0 0.0007 0 0.0015 0 0.0034 0.0028 0.0044 0 0.0003 0 0.0009 0.0008
Endosome 0.0068 0 0.0011 0 0.0107 0 0.014 0 0.0084 0.0002 0.0157 0.0081 0.0131 0 0.001 0 0.0064 0.002
Golgi 0.0021 0 0.0005 0 0.0022 0 0.004 0 0.0037 0.0002 0.0044 0.0014 0.0037 0 0.0007 0 0.0032 0.0002
Lipid Particles 0.0123 0 0.0021 0 0.0056 0 0.0052 0 0.0038 0.0041 0.004 0.0008 0.0076 0 0.0033 0 0.0314 0.0011
Mitochondria 0.0103 0.0002 0.0007 0.0001 0.0016 0.0002 0.0037 0.0003 0.0083 0.0147 0.0196 0.0017 0.0024 0.0003 0.0017 0.0004 0.0239 0.0003
None 0.0039 0 0.0008 0 0.0015 0 0.0006 0 0.0012 0.0007 0.0016 0.0004 0.0088 0 0.0025 0 0.016 0.0002
Nuclear Periphery 0.0097 0.0002 0.0018 0.0009 0.0068 0.0001 0.0031 0.0002 0.0083 0.0012 0.0064 0.0095 0.0154 0.0003 0.0021 0.0016 0.004 0.0043
Nucleolus 0.0861 0.043 0.0373 0.0124 0.281 0.151 0.0693 0.0261 0.0379 0.0507 0.2849 0.1488 0.0224 0.0315 0.0201 0.089 0.2667 0.1309
Nucleus 0.8205 0.9556 0.9397 0.9865 0.6597 0.8478 0.8568 0.9719 0.9016 0.9189 0.5979 0.8109 0.8708 0.9675 0.9501 0.9082 0.5343 0.8492
Peroxisomes 0.0047 0 0.0003 0 0.0007 0 0.0024 0 0.0009 0.0006 0.002 0.0011 0.004 0 0.0018 0 0.0115 0.0002
Punctate Nuclear 0.0038 0.0005 0.0047 0.0001 0.015 0.0001 0.0224 0.0013 0.005 0.0025 0.0047 0.0045 0.0148 0.0003 0.0038 0.0001 0.0221 0.0073
Vacuole 0.0023 0 0.0005 0 0.0014 0.0001 0.0006 0 0.0009 0.0005 0.005 0.0009 0.0027 0 0.0005 0.0001 0.0035 0.0002
Vacuole Periphery 0.0019 0 0.0005 0 0.0008 0.0001 0.0006 0 0.0013 0.0018 0.0046 0.002 0.0012 0.0001 0.0007 0.0003 0.0067 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.6123 40.947 20.156 28.9082 34.3982 17.6773 40.2742 41.3614 28.5946 36.3838
Translational Efficiency 1.8525 1.9067 2.3593 1.6346 1.709 2.5751 1.7143 1.4831 1.7868 1.6397

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
804 1797 223 1175 2217 1284 1971 86 3021 3081 2194 1261

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 989.72 1379.99 1774.78 1591.96 1258.23 1330.51 1659.56 1831.43 1186.77 1359.37 1671.27 1608.29
Standard Deviation 225.69 323.07 362.99 385.26 278.69 276.91 306.75 371.83 290.92 305.66 314.86 389.07
Intensity Change Log 2 0.479565 0.842548 0.685712 0.080584 0.399405 0.541575 0.269949 0.611423 0.606816

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000496 0.000433 0.001077 0.000851 0.000450 0.000897 0.000514 0.001107 0.000462 0.000627 0.000572 0.000868
Bud Neck 0.002365 0.003791 0.009198 0.011244 0.002693 0.004702 0.006615 0.011320 0.002606 0.004171 0.006877 0.011250
Bud Site 0.000305 0.000295 0.000983 0.001876 0.000240 0.001533 0.000365 0.001910 0.000257 0.000811 0.000428 0.001878
Cell Periphery 0.000067 0.000091 0.000029 0.000164 0.000080 0.000235 0.000166 0.000059 0.000077 0.000151 0.000152 0.000157
Cytoplasm 0.000651 0.000030 0.000041 0.000218 0.000106 0.000327 0.000049 0.000144 0.000251 0.000154 0.000048 0.000213
Cytoplasmic Foci 0.000097 0.000123 0.000188 0.000658 0.000015 0.000639 0.000121 0.000978 0.000037 0.000338 0.000128 0.000680
Eisosomes 0.000071 0.000162 0.000184 0.000059 0.000243 0.000189 0.000151 0.000072 0.000197 0.000173 0.000154 0.000060
Endoplasmic Reticulum 0.000907 0.000734 0.000631 0.000376 0.001021 0.001322 0.000443 0.000652 0.000991 0.000979 0.000462 0.000395
Endosome 0.000042 0.000069 0.000019 0.001489 0.000015 0.000388 0.000029 0.003160 0.000022 0.000202 0.000028 0.001603
Golgi 0.000049 0.000061 0.000090 0.001327 0.000017 0.000242 0.000023 0.000259 0.000025 0.000136 0.000030 0.001254
Lipid Particles 0.000177 0.000054 0.000017 0.000247 0.000003 0.000557 0.000014 0.000197 0.000049 0.000264 0.000014 0.000243
Mitochondria 0.001324 0.000628 0.002578 0.005217 0.000563 0.001952 0.001153 0.004464 0.000765 0.001179 0.001298 0.005166
Mitotic Spindle 0.000545 0.000407 0.000651 0.003155 0.000050 0.002770 0.000053 0.009915 0.000181 0.001392 0.000114 0.003616
None 0.003513 0.000478 0.000770 0.001191 0.000489 0.000822 0.000423 0.000771 0.001294 0.000621 0.000459 0.001162
Nuclear Periphery 0.000045 0.000066 0.000061 0.000363 0.000030 0.000258 0.000041 0.000397 0.000034 0.000146 0.000043 0.000365
Nuclear Periphery Foci 0.000080 0.000066 0.000013 0.000306 0.000009 0.000153 0.000013 0.000322 0.000028 0.000102 0.000013 0.000307
Nucleolus 0.078004 0.051847 0.082863 0.066368 0.052521 0.060574 0.027609 0.119876 0.059303 0.055484 0.033225 0.070017
Nucleus 0.908792* 0.938893* 0.887479* 0.899889* 0.939712* 0.918121* 0.960769* 0.829803* 0.931483* 0.930236* 0.953320* 0.895109*
Peroxisomes 0.002198 0.000995 0.012505 0.002407 0.000911 0.002506 0.001102 0.007200 0.001254 0.001625 0.002261 0.002733
Vacuole 0.000128 0.000455 0.000412 0.001812 0.000518 0.001124 0.000218 0.003795 0.000414 0.000734 0.000237 0.001947
Vacuole Periphery 0.000144 0.000323 0.000210 0.000784 0.000313 0.000689 0.000130 0.003600 0.000268 0.000475 0.000138 0.000976

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.47 -9.89 -6.98 -8.16 2.77 -3.29 -4.53 -4.41 -1.91 -4.07 -2.86 -7.68 -8.75 -3.68 -6.41
Bud Neck -4.85 -9.62 -7.76 -6.58 -1.98 -5.62 -16.97 -4.51 -3.69 -2.98 -6.98 -19.90 -8.20 -6.80 -4.52
Bud Site 0.14 -7.31 -4.14 -4.16 -2.18 -2.33 -2.90 -2.65 -1.01 -2.50 -2.38 -4.37 -4.67 -2.52 -4.13
Cell Periphery -0.70 2.50 -1.98 -1.39 -2.74 -1.13 -0.73 0.21 1.18 0.79 -1.21 -0.72 -1.76 -0.19 -0.16
Cytoplasm 1.54 1.50 1.01 -4.64 -4.12 -1.00 0.72 0.01 1.11 -1.76 0.43 1.56 0.34 -0.24 -3.97
Cytoplasmic Foci -0.20 -2.19 -2.58 -2.27 -1.84 -1.91 -1.03 -1.57 -0.44 -1.28 -2.01 -1.04 -3.21 -1.20 -2.02
Eisosomes -4.54 -7.91 1.50 5.09 8.77 1.68 2.91 5.86 5.01 3.23 1.02 1.72 6.89 7.66 5.19
Endoplasmic Reticulum 1.95 2.22 6.86 6.02 3.06 -1.73 8.02 0.74 1.68 -1.58 0.07 9.08 9.39 7.00 1.30
Endosome -0.66 2.12 -2.60 -2.54 -2.64 -2.08 -0.75 -1.06 -0.89 -1.05 -2.30 -0.54 -2.84 -2.45 -2.80
Golgi -0.25 -3.58 -1.87 -1.85 -1.77 -1.95 -1.38 -2.57 -0.58 -2.48 -2.07 -1.38 -1.94 -1.74 -1.92
Lipid Particles 0.71 0.94 -0.25 -1.10 -1.37 -1.97 -0.96 -1.84 1.12 -1.67 -1.68 0.60 -1.20 0.19 -1.44
Mitochondria 1.19 -1.85 -3.03 -4.01 -2.11 -2.42 -14.78 -3.51 -2.01 -2.96 -1.47 -3.46 -4.08 -3.63 -3.58
Mitotic Spindle 0.36 -0.36 -1.98 -2.14 -1.78 -2.36 -0.42 -1.40 -1.05 -1.40 -2.30 0.35 -2.73 -1.67 -2.76
None 2.94 2.56 2.18 -2.77 -1.16 -1.99 1.01 -3.27 -0.40 -3.77 2.34 2.93 0.33 -2.20 -2.89
Nuclear Periphery -0.57 -1.56 -3.89 -3.38 -3.61 -1.33 -1.97 -2.39 -0.90 -2.32 -1.50 -1.84 -4.39 -2.11 -4.23
Nuclear Periphery Foci 0.30 1.66 -2.07 -2.21 -2.83 -2.27 -0.59 -1.76 -0.96 -1.72 -2.19 0.54 -3.00 -1.91 -3.09
Nucleolus 5.27 -1.31 1.20 -3.88 1.97 -2.09 9.09 -4.12 -3.75 -5.18 1.33 9.23 -3.62 -4.39 -8.98
Nucleus -5.56 2.66 2.06 7.18 -1.41 4.60 -7.07 4.97 4.17 5.65 0.53 -6.75 7.39 6.96 10.63
Peroxisomes 3.40 -5.69 -0.89 -5.11 5.51 -3.04 -2.28 -3.31 -2.65 -3.22 -1.54 -4.68 -5.19 -3.57 -1.48
Vacuole -2.18 -5.64 -3.00 -2.33 -2.43 -1.41 2.50 -1.10 -0.85 -1.21 -1.53 1.86 -2.70 -1.99 -3.02
Vacuole Periphery -2.88 -4.01 -4.03 -2.69 -3.53 -1.57 8.34 -1.02 -0.87 -1.09 -1.95 7.71 -2.87 -1.78 -3.43
Standard name
Human Ortholog
Description RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Reb1

Reb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Reb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available