Standard name
Human Ortholog
Description Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.93 – 0.97 0.97 0.97 0.88 0.86 0.77 0.84 0.74 0.66 1.0 0.99 0.99 – 0.83 0.91 0.75 0.8 0.67 0.78 0.78 0.75
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0.05 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.09 – 0.1 0.09 0.09 0.29 0.19 0.4 0.35 0.41 0.55 0 0 0 – 0 0 0.05 0.07 0.18 0.06 0.05 0.06
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0.13 0.08 0.05 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 3 0 2 4 5
Bud 0 1 – 0 2 2 0 2 1 0 2 1 0 1 0 – 0 0 0 0 0 3 3 14
Bud Neck 0 1 – 0 3 1 2 6 1 2 0 1 0 0 0 – 1 0 0 2 0 3 0 4
Bud Site 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 – 2 0 0 0 0 2 2 0 1 1 2 2 – 3 1 0 0 0 2 2 3
Cytoplasm 22 140 – 83 116 116 130 125 70 94 64 50 426 472 524 – 120 71 51 119 30 210 205 247
Endoplasmic Reticulum 0 1 – 0 0 0 0 1 1 0 0 1 0 2 1 – 8 3 3 0 0 2 3 3
Endosome 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 2 0 0 4 1 1 3 3
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 – 1 0 0 0 0 0 0 0
Mitochondria 4 13 – 9 11 11 42 27 36 39 35 42 0 2 0 – 2 0 3 10 8 14 11 20
Nucleus 1 1 – 1 1 1 2 3 1 1 0 2 0 3 2 – 7 3 0 0 0 0 1 0
Nuclear Periphery 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 1 1 1 3 2 1 0 2 1 0 1 1 – 2 0 1 0 0 3 3 4
Vac/Vac Membrane 0 1 – 0 0 0 2 0 0 1 0 0 0 0 1 – 19 6 3 6 0 16 12 14
Unique Cell Count 25 151 86 120 120 147 145 91 112 86 76 426 479 530 144 78 69 150 46 272 263 329
Labelled Cell Count 29 160 96 134 132 181 167 113 140 103 99 427 484 532 165 84 69 150 46 272 263 329


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.7 – 3.1 3.1 3.1 2.4 3.1 2.6 2.3 2.4 2.4 4.1 4.0 4.1 4.6 5.6 5.4 3.7 3.9 4.5
Std Deviation (1e-4) 1.7 0.9 – 1.5 1.2 1.2 1.0 1.7 1.0 1.0 1.1 1.0 1.2 1.2 1.4 1.1 1.3 1.5 1.4 1.6 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2896 1.7733 2.0344 2.1579 2.6299 1.8766 1.0047 0.9464 1.028 1.4442 0.8941 1.073 1.8887 1.2368 1.1469 0.7618 2.0872 1.1507
Actin 0.0248 0.0002 0.0099 0.0042 0.0156 0.0234 0.0122 0.0003 0.0024 0.0117 0.0165 0.0011 0.0727 0.011 0.0268 0.001 0.0033 0.0025
Bud 0.0004 0.0009 0.002 0.007 0.0003 0.0004 0.0002 0.0132 0.0002 0.0002 0.0015 0.0001 0.0025 0.0037 0.0062 0.001 0.0003 0.0118
Bud Neck 0.0013 0.0001 0.0001 0.0005 0.0008 0.0006 0.0016 0.0001 0 0.0002 0.0002 0.0003 0.0068 0.0008 0.0009 0.0003 0 0.0003
Bud Periphery 0.0006 0.001 0.0099 0.0068 0.0003 0.0009 0.0002 0.0032 0.0004 0.0002 0.006 0.0001 0.0182 0.0338 0.0059 0.0014 0.0041 0.0039
Bud Site 0.0023 0.0077 0.0019 0.0118 0.0003 0.0005 0.0032 0.0024 0.0001 0.0035 0.0032 0.0001 0.0295 0.0049 0.0018 0.001 0 0.0003
Cell Periphery 0.0002 0.0001 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0009 0 0.0113 0.0015 0.0002 0.0001 0.0003 0.0001
Cytoplasm 0.0077 0.0206 0.0134 0.0159 0.0564 0.0327 0.0086 0.0091 0.0047 0.0047 0.0105 0.0087 0.0113 0.0167 0.0112 0.0229 0.0073 0.0268
Cytoplasmic Foci 0.0219 0.0073 0.0071 0.0092 0.0069 0.0238 0.0792 0.0006 0.0017 0.0378 0.0043 0.0152 0.0184 0.005 0.0051 0.0073 0.0007 0.0039
Eisosomes 0.0003 0.0002 0.0004 0.0001 0.0001 0.0002 0.0009 0.0006 0.0004 0.0002 0.0017 0.0001 0.0067 0.0008 0.0005 0.0001 0.0013 0.0001
Endoplasmic Reticulum 0.0022 0.0006 0.0024 0.0011 0.0004 0.0004 0.036 0.0003 0.003 0.0008 0.0038 0.0001 0.0077 0.0021 0.001 0.0005 0.0005 0.0002
Endosome 0.0122 0.0031 0.0137 0.0287 0.0125 0.0028 0.0279 0 0.0087 0.0708 0.0115 0.0081 0.0255 0.0044 0.0011 0.0003 0.0001 0.0008
Golgi 0.004 0.0004 0.0034 0.0115 0.0415 0.0036 0.0043 0 0.0009 0.0207 0.0069 0.0106 0.013 0.0013 0.0013 0.0001 0.0001 0.0006
Lipid Particles 0.0125 0.0013 0.0017 0.0012 0.0013 0.0062 0.0087 0.0001 0.0011 0.0095 0.0027 0.0003 0.0278 0.0012 0.0009 0.0003 0.0002 0.002
Mitochondria 0.002 0.0002 0.0328 0.0082 0.01 0.0044 0.0071 0.0004 0.0011 0.0015 0.0603 0.0023 0.0122 0.0017 0.0032 0.0003 0.0007 0.0004
None 0.8554 0.9508 0.8924 0.8798 0.8516 0.8837 0.7194 0.9363 0.965 0.7947 0.8622 0.9504 0.7032 0.9029 0.9255 0.9593 0.9804 0.9385
Nuclear Periphery 0.0066 0.0003 0.004 0.0008 0.0001 0.0007 0.0417 0.0015 0.006 0.0006 0.0014 0 0.0136 0.0018 0.0015 0.0003 0.0001 0.0003
Nucleolus 0.0014 0.0007 0.0001 0.0002 0 0.0003 0.0002 0.0016 0.0004 0.0001 0.0003 0 0.0007 0.0007 0.0008 0.0002 0.0001 0.0002
Nucleus 0.0019 0.0007 0.0003 0.0009 0.0001 0.0002 0.0035 0.026 0.0009 0.0001 0.0008 0 0.0026 0.0014 0.0017 0.0005 0.0001 0.0012
Peroxisomes 0.0119 0.0004 0.001 0.001 0.0008 0.0129 0.0374 0 0.0004 0.0386 0.0018 0.0009 0.0082 0.0006 0.0026 0.0003 0.0001 0.004
Punctate Nuclear 0.0262 0.003 0.0006 0.0004 0.0001 0.0016 0.0034 0.0037 0.001 0.0019 0.0002 0.0012 0.0042 0.0025 0.0013 0.0027 0.0001 0.0015
Vacuole 0.0028 0.0004 0.0012 0.0082 0.0004 0.0003 0.0017 0.0003 0.0008 0.0012 0.0019 0.0001 0.0023 0.0011 0.0004 0.0002 0.0001 0.0005
Vacuole Periphery 0.0014 0.0001 0.0014 0.002 0.0003 0.0002 0.0024 0.0001 0.0008 0.0009 0.0012 0.0001 0.0015 0.0003 0.0002 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.9752 42.8554 244.4992 168.643 49.9288 57.339 83.1103 175.2334 230.7407 104.1635
Translational Efficiency 0.4517 0.4944 0.7119 0.8125 0.7694 0.5313 0.7199 0.8054 0.9394 0.8264

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1604 1034 2328 348 2284 2334 839 381 3888 3368 3167 729

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 624.10 680.20 750.76 817.65 657.68 702.73 813.59 780.58 643.83 695.81 767.40 798.28
Standard Deviation 83.32 81.79 77.93 93.70 70.38 87.61 97.76 129.07 77.76 86.49 88.12 115.06
Intensity Change Log 2 — 0.124182 0.266575 0.389706 — 0.095585 0.306916 0.247161 — 0.109579 0.287415 0.318326

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000105 0.000289 0.000054 0.000419 0.000084 0.000410 0.000110 0.000233 0.000093 0.000373 0.000069 0.000322
Bud Neck 0.052265 0.035527 0.004338 0.014787 0.016731 0.054398 0.002438 0.006820 0.031390 0.048604 0.003835 0.010623
Bud Site 0.014821 0.012618 0.006591 0.028393 0.003130 0.019211 0.003111 0.015927 0.007953 0.017187 0.005669 0.021878
Cell Periphery 0.010285 0.000129 0.000147 0.000140 0.000248 0.000140 0.000149 0.000256 0.004389 0.000137 0.000148 0.000201
Cytoplasm 0.430144* 0.362943* 0.359433* 0.436058* 0.496385* 0.405923* 0.505159* 0.425611* 0.469057* 0.392728* 0.398039* 0.430598*
Cytoplasmic Foci 0.084049 0.282068* 0.011404 0.022634 0.185729* 0.213559* 0.026724 0.040638 0.143781 0.234592* 0.015463 0.032043
Eisosomes 0.000567 0.000160 0.000027 0.000074 0.000212 0.000172 0.000056 0.000104 0.000358 0.000168 0.000035 0.000089
Endoplasmic Reticulum 0.003020 0.000725 0.000610 0.003154 0.000733 0.000692 0.001161 0.000898 0.001676 0.000702 0.000756 0.001975
Endosome 0.007499 0.038992 0.001165 0.005786 0.007406 0.024230 0.002565 0.012884 0.007444 0.028762 0.001536 0.009496
Golgi 0.000921 0.004597 0.000055 0.000167 0.001501 0.006307 0.000127 0.007727 0.001262 0.005782 0.000074 0.004118
Lipid Particles 0.006223 0.008436 0.000736 0.002289 0.006649 0.006287 0.001697 0.001689 0.006473 0.006947 0.000990 0.001975
Mitochondria 0.001383 0.009450 0.000568 0.002776 0.002145 0.019695 0.000409 0.001499 0.001830 0.016550 0.000526 0.002109
Mitotic Spindle 0.000539 0.004200 0.002426 0.014052 0.000209 0.011444 0.001027 0.027404 0.000345 0.009220 0.002055 0.021030
None 0.005968 0.002958 0.006154 0.010040 0.002854 0.002150 0.005390 0.014592 0.004139 0.002398 0.005952 0.012419
Nuclear Periphery 0.000435 0.000565 0.001232 0.001644 0.000333 0.000477 0.000883 0.000900 0.000375 0.000504 0.001140 0.001255
Nuclear Periphery Foci 0.000221 0.000404 0.000124 0.001343 0.000142 0.000211 0.000784 0.000619 0.000175 0.000271 0.000299 0.000965
Nucleolus 0.000900 0.002926 0.000686 0.000700 0.000664 0.001736 0.000476 0.000860 0.000762 0.002101 0.000630 0.000783
Nucleus 0.216630* 0.123096 0.587363* 0.243831* 0.224584* 0.123843 0.382148* 0.318188* 0.221303* 0.123614 0.532998* 0.282692*
Peroxisomes 0.001620 0.018358 0.000171 0.002169 0.004115 0.019182 0.000436 0.000976 0.003086 0.018929 0.000241 0.001545
Vacuole 0.161436 0.089175 0.016369 0.207808* 0.045730 0.087834 0.065004 0.115907 0.093465 0.088246 0.029253 0.159777
Vacuole Periphery 0.000966 0.002383 0.000348 0.001737 0.000417 0.002101 0.000146 0.006267 0.000643 0.002188 0.000294 0.004105

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.65 1.82 -4.29 -0.32 -5.60 -8.40 -2.50 -5.08 2.44 -3.08 -9.65 0.52 -6.69 1.99 -7.06
Bud Neck 4.07 13.33 11.23 9.05 -1.31 -14.49 11.84 5.78 17.57 -2.83 -6.95 16.48 12.33 19.49 -2.35
Bud Site 1.25 4.60 1.09 0.38 -1.66 -11.75 0.12 -3.70 1.43 -3.71 -7.53 2.56 -2.18 1.79 -3.41
Cell Periphery 7.35 7.35 7.37 2.39 2.48 1.43 1.41 0.82 -2.19 -2.11 7.37 7.37 7.35 -0.77 -0.55
Cytoplasm 6.52 10.76 7.99 3.32 1.99 11.64 4.29 10.60 4.32 6.47 12.22 15.87 13.58 6.12 3.83
Cytoplasmic Foci -26.11 24.54 18.25 36.09 -2.71 -4.38 36.22 31.16 34.37 -1.79 -18.92 44.66 35.65 49.53 -4.89
Eisosomes 10.59 14.46 12.79 6.87 -11.42 2.08 9.48 6.29 4.26 -3.53 9.36 17.79 14.11 6.55 -9.93
Endoplasmic Reticulum 4.45 4.17 -1.04 -9.30 -8.90 0.21 -6.35 -5.45 -5.78 -0.65 4.14 2.27 -3.95 -10.56 -8.58
Endosome -11.49 10.48 5.45 13.08 -5.79 -11.75 6.63 -0.07 7.47 -2.64 -16.50 12.31 1.64 14.85 -4.19
Golgi -5.16 3.22 3.01 6.93 -3.16 -7.41 8.20 -1.32 1.45 -2.19 -9.00 8.04 -0.81 3.79 -2.23
Lipid Particles -3.22 13.68 9.91 11.25 -7.34 0.76 12.48 11.86 10.02 -1.02 -1.07 20.21 15.52 14.54 -7.40
Mitochondria -6.73 3.59 0.83 6.77 -1.10 -12.74 8.65 3.15 13.35 -2.62 -14.34 8.58 2.80 14.83 -2.25
Mitotic Spindle -2.54 -3.24 -2.96 -1.93 -2.40 -7.72 -2.61 -4.04 -2.11 -3.82 -8.01 -4.58 -4.99 -2.47 -4.41
None 3.24 0.21 0.53 -1.55 0.43 1.79 -2.46 -2.34 -2.58 -1.78 4.23 -2.62 -2.05 -3.10 -1.26
Nuclear Periphery -1.51 -13.43 -13.23 -12.36 -6.76 -3.47 -16.29 -9.53 -8.41 -4.76 -3.15 -21.44 -15.95 -14.52 -7.04
Nuclear Periphery Foci -3.75 1.49 -7.53 -6.23 -7.92 -2.33 -10.33 -7.45 -6.52 1.88 -3.76 -7.87 -10.25 -9.04 -7.64
Nucleolus -4.79 0.88 2.54 5.76 1.82 -5.74 3.76 -0.57 4.61 -2.44 -7.22 1.03 1.38 7.59 0.33
Nucleus 12.64 -48.22 -5.22 -11.52 20.76 18.81 -15.80 -7.10 -14.15 3.62 22.53 -51.63 -8.71 -18.26 21.26
Peroxisomes -11.90 4.24 2.08 12.73 -2.41 -11.93 8.86 8.00 15.48 -1.52 -16.36 10.18 7.67 19.53 -3.33
Vacuole 8.72 20.27 -9.88 -14.59 -19.43 -12.63 -10.86 -12.75 -9.54 -6.92 0.73 12.16 -16.39 -16.95 -20.98
Vacuole Periphery -3.39 1.86 0.84 5.05 -1.10 -9.90 8.50 -1.88 -0.70 -2.08 -7.85 2.31 -1.65 0.34 -2.18
Standard name
Human Ortholog
Description Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
Localization
Cell Percentages cytoplasm (31%), vacuole (4%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pho5

Pho5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available