Standard name | |
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Human Ortholog | |
Description | Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 2 | 0 | 3 | 4 | 5 | 0 | 4 | 8 |
Bud | 2 | 3 | 6 | 7 | 8 | 15 | 29 | 19 | 11 | 11 | 13 | 18 | 2 | 1 | 0 | 10 | 15 | 1 | 1 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 2 | 10 | 6 | 3 | 5 | 6 | 8 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 9 | 2 | 2 | 1 | 0 | 0 | 0 | 4 | 1 | 0 | 3 | 2 | 10 | 10 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 40 | 41 | 35 | 52 | 37 | 45 | 45 | 39 | 33 | 28 | 19 | 23 | 83 | 130 | 149 | 36 | 34 | 29 | 10 | 12 | 3 | 7 | 18 | 25 |
Endoplasmic Reticulum | 2 | 2 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 9 | 5 | 6 | 4 | 1 | 2 | 0 | 1 | 1 |
Endosome | 0 | 2 | 15 | 6 | 14 | 0 | 0 | 6 | 5 | 0 | 0 | 0 | 7 | 6 | 9 | 37 | 32 | 16 | 9 | 10 | 39 | 3 | 8 | 6 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 2 | 1 | 0 | 5 | 0 | 0 | 1 |
Mitochondria | 95 | 49 | 102 | 99 | 125 | 215 | 302 | 238 | 171 | 227 | 192 | 231 | 4 | 0 | 1 | 38 | 27 | 16 | 73 | 95 | 181 | 37 | 76 | 108 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 7 | 3 | 5 | 1 | 0 | 3 | 1 | 1 | 0 | 7 | 5 | 4 | 0 | 0 | 0 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 0 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 31 | 18 | 17 | 4 | 7 | 5 | 4 | 1 | 1 | 1 | 6 | 0 | 2 | 14 | 16 | 25 | 2 | 1 | 21 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 32 | 10 | 17 | 15 | 21 | 19 | 11 | 11 | 7 | 13 | 1 | 4 | 3 | 29 | 31 | 27 | 2 | 0 | 4 | 1 | 5 | 7 |
Vac/Vac Membrane | 2 | 4 | 11 | 2 | 8 | 10 | 12 | 8 | 12 | 5 | 5 | 6 | 3 | 4 | 5 | 29 | 23 | 24 | 5 | 9 | 3 | 1 | 11 | 13 |
Unique Cell Count | 119 | 101 | 198 | 162 | 192 | 251 | 346 | 276 | 204 | 246 | 208 | 247 | 104 | 147 | 167 | 181 | 161 | 129 | 121 | 141 | 278 | 58 | 141 | 191 |
Labelled Cell Count | 142 | 108 | 243 | 207 | 234 | 309 | 436 | 345 | 256 | 296 | 253 | 308 | 111 | 155 | 175 | 227 | 206 | 161 | 121 | 141 | 278 | 58 | 141 | 191 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.2 | 4.2 | 4.7 | 4.1 | 3.5 | 3.3 | 3.4 | 4.0 | 3.1 | 3.3 | 3.0 | 5.8 | 5.6 | 5.5 | 6.0 | 5.8 | 5.8 | 5.3 | 6.1 | 6.2 |
Std Deviation (1e-4) | 0.8 | 0.5 | 1.3 | 3.8 | 1.8 | 1.6 | 1.5 | 1.7 | 3.2 | 1.4 | 2.6 | 1.3 | 1.9 | 1.4 | 1.6 | 1.5 | 1.5 | 1.6 | 0.9 | 2.0 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.17 | -0.03 | -0.27 | -0.34 | -0.3 | -0.06 | -0.44 | -0.33 | -0.48 | 0.47 | 0.4 | 0.39 | 0.52 | 0.46 | 0.47 | 0.32 | 0.54 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.3 | 2.8 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9873 | -0.46 | -0.7001 | -0.7159 | -0.9452 | -0.6307 | -0.512 | -0.2217 | -0.0968 | 0.0265 | -0.2213 | -0.2344 | -0.274 | -0.2429 | -0.2687 | -0.4039 | -0.3866 | -0.5893 |
Actin | 0.0383 | 0.0178 | 0.0087 | 0.0029 | 0.0145 | 0.0122 | 0.0272 | 0.0181 | 0.012 | 0.0164 | 0.0273 | 0.015 | 0.0679 | 0.0155 | 0.0431 | 0.0257 | 0.1077 | 0.0171 |
Bud | 0.0022 | 0.001 | 0.0017 | 0.0014 | 0.0073 | 0.0027 | 0.0041 | 0.0043 | 0.0032 | 0.007 | 0.0049 | 0.0026 | 0.0017 | 0.0052 | 0.0031 | 0.0037 | 0.0018 | 0.0019 |
Bud Neck | 0.0019 | 0.001 | 0.0016 | 0.0014 | 0.0111 | 0.013 | 0.0038 | 0.0033 | 0.0109 | 0.0056 | 0.007 | 0.0056 | 0.0015 | 0.002 | 0.0027 | 0.0033 | 0.0012 | 0.0025 |
Bud Periphery | 0.0028 | 0.0016 | 0.0038 | 0.0052 | 0.0102 | 0.0043 | 0.005 | 0.0056 | 0.01 | 0.0255 | 0.0044 | 0.0029 | 0.0038 | 0.0148 | 0.011 | 0.0136 | 0.0044 | 0.0066 |
Bud Site | 0.006 | 0.0122 | 0.0108 | 0.0022 | 0.0205 | 0.0022 | 0.0161 | 0.0466 | 0.0152 | 0.0188 | 0.0168 | 0.0014 | 0.0048 | 0.0365 | 0.0079 | 0.0043 | 0.0017 | 0.0012 |
Cell Periphery | 0.0013 | 0.0013 | 0.001 | 0.0017 | 0.0008 | 0.0009 | 0.0023 | 0.0019 | 0.0024 | 0.0027 | 0.0003 | 0.0008 | 0.0016 | 0.0026 | 0.0028 | 0.0028 | 0.0005 | 0.001 |
Cytoplasm | 0.0143 | 0.0378 | 0.0207 | 0.0297 | 0.0461 | 0.0247 | 0.0557 | 0.0621 | 0.0622 | 0.0611 | 0.0423 | 0.0699 | 0.0354 | 0.0737 | 0.0796 | 0.0834 | 0.0349 | 0.0509 |
Cytoplasmic Foci | 0.1047 | 0.1243 | 0.1073 | 0.1395 | 0.1174 | 0.1151 | 0.1142 | 0.1205 | 0.1115 | 0.1004 | 0.1455 | 0.1379 | 0.1392 | 0.1428 | 0.0882 | 0.1296 | 0.1459 | 0.0805 |
Eisosomes | 0.0016 | 0.0008 | 0.0016 | 0.0006 | 0.0007 | 0.0005 | 0.0009 | 0.001 | 0.0007 | 0.0009 | 0.0002 | 0.0004 | 0.0023 | 0.0014 | 0.0011 | 0.0007 | 0.0008 | 0.0005 |
Endoplasmic Reticulum | 0.002 | 0.003 | 0.0014 | 0.0023 | 0.0013 | 0.0009 | 0.0114 | 0.0036 | 0.003 | 0.0056 | 0.0063 | 0.0056 | 0.0101 | 0.006 | 0.0133 | 0.003 | 0.0029 | 0.0054 |
Endosome | 0.1131 | 0.0922 | 0.0908 | 0.0783 | 0.0879 | 0.0753 | 0.1426 | 0.1228 | 0.1445 | 0.1284 | 0.134 | 0.1423 | 0.1705 | 0.1442 | 0.1123 | 0.1093 | 0.1454 | 0.153 |
Golgi | 0.0521 | 0.0395 | 0.0463 | 0.0389 | 0.0205 | 0.0479 | 0.0624 | 0.0529 | 0.0655 | 0.0316 | 0.0593 | 0.065 | 0.0691 | 0.0782 | 0.0566 | 0.0298 | 0.0582 | 0.0642 |
Lipid Particles | 0.0581 | 0.0419 | 0.0545 | 0.0248 | 0.0696 | 0.093 | 0.043 | 0.031 | 0.0218 | 0.0542 | 0.0184 | 0.0247 | 0.0568 | 0.0388 | 0.0153 | 0.0157 | 0.0585 | 0.0313 |
Mitochondria | 0.3671 | 0.4085 | 0.4057 | 0.4488 | 0.4005 | 0.3694 | 0.2483 | 0.2843 | 0.3636 | 0.379 | 0.3224 | 0.3227 | 0.3191 | 0.2521 | 0.3597 | 0.3539 | 0.1388 | 0.483 |
None | 0.1161 | 0.1254 | 0.1178 | 0.1379 | 0.0841 | 0.0813 | 0.1921 | 0.1708 | 0.0947 | 0.0516 | 0.1316 | 0.1144 | 0.0648 | 0.1473 | 0.1525 | 0.1178 | 0.1073 | 0.0468 |
Nuclear Periphery | 0.0019 | 0.0014 | 0.0023 | 0.0042 | 0.0021 | 0.0018 | 0.0058 | 0.0012 | 0.0015 | 0.0019 | 0.002 | 0.0033 | 0.0046 | 0.001 | 0.0072 | 0.0013 | 0.0005 | 0.0014 |
Nucleolus | 0.0037 | 0.0013 | 0.003 | 0.0025 | 0.0075 | 0.002 | 0.0015 | 0.0043 | 0.0078 | 0.0049 | 0.0007 | 0.0005 | 0.0007 | 0.0013 | 0.0007 | 0.0003 | 0.0004 | 0.0004 |
Nucleus | 0.0037 | 0.0016 | 0.0023 | 0.0045 | 0.0123 | 0.004 | 0.0025 | 0.0038 | 0.0042 | 0.0019 | 0.0012 | 0.0019 | 0.001 | 0.0013 | 0.0028 | 0.001 | 0.0005 | 0.0007 |
Peroxisomes | 0.0855 | 0.0754 | 0.1036 | 0.0306 | 0.0531 | 0.1365 | 0.0379 | 0.0352 | 0.0465 | 0.0686 | 0.0547 | 0.0687 | 0.0264 | 0.0158 | 0.0248 | 0.0858 | 0.1585 | 0.0316 |
Punctate Nuclear | 0.0133 | 0.0013 | 0.0044 | 0.0335 | 0.0255 | 0.0047 | 0.003 | 0.0061 | 0.0022 | 0.0016 | 0.0044 | 0.0022 | 0.0031 | 0.0018 | 0.0021 | 0.0013 | 0.0017 | 0.0004 |
Vacuole | 0.0078 | 0.0081 | 0.0076 | 0.0063 | 0.0049 | 0.0049 | 0.0158 | 0.0156 | 0.0118 | 0.0242 | 0.0118 | 0.0086 | 0.0103 | 0.0141 | 0.009 | 0.0101 | 0.0179 | 0.0117 |
Vacuole Periphery | 0.0026 | 0.0027 | 0.0031 | 0.0029 | 0.0021 | 0.0029 | 0.0043 | 0.0048 | 0.0046 | 0.008 | 0.0046 | 0.0037 | 0.0051 | 0.0037 | 0.0044 | 0.0037 | 0.0106 | 0.0078 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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