Standard name
Human Ortholog
Description Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0.06 0.08 0.07 0.05 0 0.06 0.07 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.34 0.41 0.18 0.32 0.19 0.18 0.13 0.14 0.16 0.11 0.09 0.09 0.8 0.88 0.89 0.2 0.21 0.22 0.09 0.09 0 0.13 0.13 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0.07 0 0.05 0.2 0.2 0.12 0.08 0.08 0.14 0.06 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.49 0.52 0.61 0.65 0.86 0.87 0.86 0.84 0.92 0.92 0.94 0 0 0 0.21 0.17 0.12 0.61 0.68 0.65 0.66 0.54 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.16 0.11 0.09 0 0 0 0 0 0 0 0.06 0 0 0.08 0.1 0.19 0 0 0.08 0 0 0
SpindlePole 0 0 0.16 0.06 0.09 0.06 0.06 0.07 0.05 0 0 0.05 0 0 0 0.16 0.19 0.21 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0 0.16 0.14 0.19 0.05 0.07 0 0 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 1 0 0 0 0 0 0 0 0 0 1 2 1 2 2 0 3 4 5 0 4 8
Bud 2 3 6 7 8 15 29 19 11 11 13 18 2 1 0 10 15 1 1 1 0 0 0 2
Bud Neck 1 0 1 0 2 0 0 1 0 0 0 0 1 2 1 0 3 3 0 0 0 0 2 4
Bud Site 0 0 1 1 0 2 10 6 3 5 6 8 0 0 0 1 1 1
Cell Periphery 0 2 1 9 2 2 1 0 0 0 4 1 0 3 2 10 10 7 0 0 0 0 0 0
Cytoplasm 40 41 35 52 37 45 45 39 33 28 19 23 83 130 149 36 34 29 10 12 3 7 18 25
Endoplasmic Reticulum 2 2 5 3 1 1 0 0 0 1 1 1 2 0 2 9 5 6 4 1 2 0 1 1
Endosome 0 2 15 6 14 0 0 6 5 0 0 0 7 6 9 37 32 16 9 10 39 3 8 6
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 4 0 2 1 0 5 0 0 1
Mitochondria 95 49 102 99 125 215 302 238 171 227 192 231 4 0 1 38 27 16 73 95 181 37 76 108
Nucleus 0 0 1 0 0 0 7 3 5 1 0 3 1 1 0 7 5 4 0 0 0 1 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 4 1 2 0 0 0 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 3 0 2 1 0 2 4 1 0 0 0 0 2 0 0 0 0 0 1 0
Peroxisomes 0 0 31 18 17 4 7 5 4 1 1 1 6 0 2 14 16 25 2 1 21 0 1 1
SpindlePole 0 0 32 10 17 15 21 19 11 11 7 13 1 4 3 29 31 27 2 0 4 1 5 7
Vac/Vac Membrane 2 4 11 2 8 10 12 8 12 5 5 6 3 4 5 29 23 24 5 9 3 1 11 13
Unique Cell Count 119 101 198 162 192 251 346 276 204 246 208 247 104 147 167 181 161 129 121 141 278 58 141 191
Labelled Cell Count 142 108 243 207 234 309 436 345 256 296 253 308 111 155 175 227 206 161 121 141 278 58 141 191


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.2 4.2 4.7 4.1 3.5 3.3 3.4 4.0 3.1 3.3 3.0 5.8 5.6 5.5 6.0 5.8 5.8 5.3 6.1 6.2
Std Deviation (1e-4) 0.8 0.5 1.3 3.8 1.8 1.6 1.5 1.7 3.2 1.4 2.6 1.3 1.9 1.4 1.6 1.5 1.5 1.6 0.9 2.0 1.5
Intensity Change (Log2) 0.17 -0.03 -0.27 -0.34 -0.3 -0.06 -0.44 -0.33 -0.48 0.47 0.4 0.39 0.52 0.46 0.47 0.32 0.54 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.3 2.8 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9873 -0.46 -0.7001 -0.7159 -0.9452 -0.6307 -0.512 -0.2217 -0.0968 0.0265 -0.2213 -0.2344 -0.274 -0.2429 -0.2687 -0.4039 -0.3866 -0.5893
Actin 0.0383 0.0178 0.0087 0.0029 0.0145 0.0122 0.0272 0.0181 0.012 0.0164 0.0273 0.015 0.0679 0.0155 0.0431 0.0257 0.1077 0.0171
Bud 0.0022 0.001 0.0017 0.0014 0.0073 0.0027 0.0041 0.0043 0.0032 0.007 0.0049 0.0026 0.0017 0.0052 0.0031 0.0037 0.0018 0.0019
Bud Neck 0.0019 0.001 0.0016 0.0014 0.0111 0.013 0.0038 0.0033 0.0109 0.0056 0.007 0.0056 0.0015 0.002 0.0027 0.0033 0.0012 0.0025
Bud Periphery 0.0028 0.0016 0.0038 0.0052 0.0102 0.0043 0.005 0.0056 0.01 0.0255 0.0044 0.0029 0.0038 0.0148 0.011 0.0136 0.0044 0.0066
Bud Site 0.006 0.0122 0.0108 0.0022 0.0205 0.0022 0.0161 0.0466 0.0152 0.0188 0.0168 0.0014 0.0048 0.0365 0.0079 0.0043 0.0017 0.0012
Cell Periphery 0.0013 0.0013 0.001 0.0017 0.0008 0.0009 0.0023 0.0019 0.0024 0.0027 0.0003 0.0008 0.0016 0.0026 0.0028 0.0028 0.0005 0.001
Cytoplasm 0.0143 0.0378 0.0207 0.0297 0.0461 0.0247 0.0557 0.0621 0.0622 0.0611 0.0423 0.0699 0.0354 0.0737 0.0796 0.0834 0.0349 0.0509
Cytoplasmic Foci 0.1047 0.1243 0.1073 0.1395 0.1174 0.1151 0.1142 0.1205 0.1115 0.1004 0.1455 0.1379 0.1392 0.1428 0.0882 0.1296 0.1459 0.0805
Eisosomes 0.0016 0.0008 0.0016 0.0006 0.0007 0.0005 0.0009 0.001 0.0007 0.0009 0.0002 0.0004 0.0023 0.0014 0.0011 0.0007 0.0008 0.0005
Endoplasmic Reticulum 0.002 0.003 0.0014 0.0023 0.0013 0.0009 0.0114 0.0036 0.003 0.0056 0.0063 0.0056 0.0101 0.006 0.0133 0.003 0.0029 0.0054
Endosome 0.1131 0.0922 0.0908 0.0783 0.0879 0.0753 0.1426 0.1228 0.1445 0.1284 0.134 0.1423 0.1705 0.1442 0.1123 0.1093 0.1454 0.153
Golgi 0.0521 0.0395 0.0463 0.0389 0.0205 0.0479 0.0624 0.0529 0.0655 0.0316 0.0593 0.065 0.0691 0.0782 0.0566 0.0298 0.0582 0.0642
Lipid Particles 0.0581 0.0419 0.0545 0.0248 0.0696 0.093 0.043 0.031 0.0218 0.0542 0.0184 0.0247 0.0568 0.0388 0.0153 0.0157 0.0585 0.0313
Mitochondria 0.3671 0.4085 0.4057 0.4488 0.4005 0.3694 0.2483 0.2843 0.3636 0.379 0.3224 0.3227 0.3191 0.2521 0.3597 0.3539 0.1388 0.483
None 0.1161 0.1254 0.1178 0.1379 0.0841 0.0813 0.1921 0.1708 0.0947 0.0516 0.1316 0.1144 0.0648 0.1473 0.1525 0.1178 0.1073 0.0468
Nuclear Periphery 0.0019 0.0014 0.0023 0.0042 0.0021 0.0018 0.0058 0.0012 0.0015 0.0019 0.002 0.0033 0.0046 0.001 0.0072 0.0013 0.0005 0.0014
Nucleolus 0.0037 0.0013 0.003 0.0025 0.0075 0.002 0.0015 0.0043 0.0078 0.0049 0.0007 0.0005 0.0007 0.0013 0.0007 0.0003 0.0004 0.0004
Nucleus 0.0037 0.0016 0.0023 0.0045 0.0123 0.004 0.0025 0.0038 0.0042 0.0019 0.0012 0.0019 0.001 0.0013 0.0028 0.001 0.0005 0.0007
Peroxisomes 0.0855 0.0754 0.1036 0.0306 0.0531 0.1365 0.0379 0.0352 0.0465 0.0686 0.0547 0.0687 0.0264 0.0158 0.0248 0.0858 0.1585 0.0316
Punctate Nuclear 0.0133 0.0013 0.0044 0.0335 0.0255 0.0047 0.003 0.0061 0.0022 0.0016 0.0044 0.0022 0.0031 0.0018 0.0021 0.0013 0.0017 0.0004
Vacuole 0.0078 0.0081 0.0076 0.0063 0.0049 0.0049 0.0158 0.0156 0.0118 0.0242 0.0118 0.0086 0.0103 0.0141 0.009 0.0101 0.0179 0.0117
Vacuole Periphery 0.0026 0.0027 0.0031 0.0029 0.0021 0.0029 0.0043 0.0048 0.0046 0.008 0.0046 0.0037 0.0051 0.0037 0.0044 0.0037 0.0106 0.0078

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
991 281 528 1388 1359 1796 364 498 2350 2077 892 1886

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.34 822.60 825.46 753.62 602.97 696.05 891.47 773.28 612.83 713.17 852.40 758.81
Standard Deviation 72.34 245.16 90.17 111.93 83.40 102.83 105.12 96.48 79.76 138.38 101.85 108.41
Intensity Change Log 2 0.393245 0.398252 0.266891 0.207105 0.564100 0.358905 0.304941 0.481982 0.312757

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000825 0.004937 0.000997 0.009212 0.000466 0.001495 0.002403 0.003135 0.000617 0.001961 0.001571 0.007607
Bud Neck 0.040056 0.035326 0.012321 0.030042 0.037556 0.035491 0.013238 0.026239 0.038611 0.035469 0.012695 0.029038
Bud Site 0.019745 0.050744 0.005704 0.072030 0.023639 0.024400 0.016955 0.069851 0.021997 0.027964 0.010295 0.071454
Cell Periphery 0.000352 0.000301 0.000295 0.000351 0.000245 0.000227 0.000232 0.000136 0.000290 0.000237 0.000269 0.000294
Cytoplasm 0.054882 0.037235 0.195892* 0.323960* 0.133985* 0.053739 0.258192* 0.378949* 0.100627* 0.051506 0.221315* 0.338480*
Cytoplasmic Foci 0.196477* 0.189971* 0.103276 0.075765 0.296464* 0.206360* 0.130960 0.218441* 0.254299* 0.204143* 0.114573 0.113438
Eisosomes 0.000232 0.000217 0.000235 0.000189 0.000190 0.000207 0.000161 0.000137 0.000208 0.000208 0.000204 0.000176
Endoplasmic Reticulum 0.001199 0.001569 0.005356 0.005404 0.002224 0.000541 0.004525 0.000452 0.001792 0.000680 0.005017 0.004096
Endosome 0.071685 0.119939 0.018742 0.014044 0.062941 0.100885 0.022682 0.026144 0.066628 0.103463 0.020350 0.017239
Golgi 0.069379 0.092741 0.001155 0.003983 0.052133 0.099403 0.002435 0.023722 0.059406 0.098502 0.001677 0.009195
Lipid Particles 0.018402 0.004250 0.016457 0.004100 0.024836 0.011989 0.006945 0.001200 0.022122 0.010942 0.012575 0.003334
Mitochondria 0.386376* 0.239320* 0.003624 0.009790 0.191781* 0.300843* 0.001883 0.010326 0.273842* 0.292520* 0.002913 0.009932
Mitotic Spindle 0.010873 0.036280 0.009213 0.032015 0.013096 0.015829 0.015809 0.022248 0.012159 0.018596 0.011905 0.029436
None 0.001727 0.000327 0.007108 0.002410 0.005982 0.001872 0.003157 0.006051 0.004187 0.001663 0.005496 0.003371
Nuclear Periphery 0.000468 0.000176 0.003006 0.001407 0.001157 0.000366 0.001926 0.000145 0.000867 0.000340 0.002565 0.001074
Nuclear Periphery Foci 0.000281 0.000332 0.002559 0.002079 0.000586 0.000354 0.002300 0.001094 0.000457 0.000351 0.002453 0.001819
Nucleolus 0.001966 0.000584 0.009996 0.001192 0.007359 0.002655 0.002397 0.001538 0.005084 0.002375 0.006895 0.001283
Nucleus 0.011314 0.005767 0.509351* 0.214215* 0.049533 0.011257 0.352660* 0.037665 0.033416 0.010514 0.445410* 0.167597*
Peroxisomes 0.080502 0.128244* 0.013201 0.035094 0.042327 0.085124 0.033030 0.082158 0.058425 0.090958 0.021293 0.047521
Vacuole 0.022759 0.043420 0.079741 0.159126 0.039718 0.036074 0.127174 0.088985 0.032566 0.037068 0.099097 0.140605
Vacuole Periphery 0.010499 0.008321 0.001770 0.003596 0.013782 0.010889 0.000939 0.001382 0.012398 0.010541 0.001431 0.003011

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.14 -1.96 -6.24 -0.89 -5.55 -4.23 -2.45 -3.58 -1.68 -0.64 -4.80 -3.36 -7.11 -4.71 -5.16
Bud Neck 0.65 7.13 3.73 1.91 -5.83 0.54 8.05 4.24 3.91 -3.08 0.99 11.00 5.62 4.57 -6.58
Bud Site -5.01 6.39 -7.70 0.03 -12.09 -0.44 2.86 -5.50 -5.35 -6.60 -3.04 7.21 -9.28 -7.09 -13.11
Cell Periphery 0.97 1.18 1.53 0.48 0.70 0.76 0.99 5.64 5.96 4.28 2.18 1.00 1.98 0.02 1.37
Cytoplasm 2.13 -13.70 -30.00 -28.51 -9.28 13.24 -7.29 -17.71 -24.23 -9.37 11.02 -13.00 -30.18 -39.28 -12.18
Cytoplasmic Foci 0.68 11.49 21.06 12.38 6.72 12.13 18.92 7.78 -0.20 -8.09 8.39 23.36 27.73 19.80 0.97
Eisosomes 0.53 -0.03 4.74 1.69 3.86 -1.20 2.05 3.78 4.46 1.85 -0.06 0.38 5.25 4.24 3.85
Endoplasmic Reticulum -0.85 -8.00 -9.90 -7.56 1.17 4.50 -6.42 4.54 0.59 12.03 4.37 -9.53 -7.10 -12.28 3.67
Endosome -4.50 12.53 16.30 11.12 4.32 -8.90 11.96 11.04 19.00 -1.35 -9.52 17.65 22.38 27.29 3.17
Golgi -2.55 18.20 17.74 11.39 -2.14 -10.69 18.12 8.26 17.59 -5.59 -9.96 25.72 21.86 27.56 -5.72
Lipid Particles 6.62 0.90 7.53 0.54 7.68 6.69 10.39 13.97 12.02 9.67 7.44 6.22 14.77 9.68 9.10
Mitochondria 7.32 34.35* 34.08* 13.29 -2.88 -10.92 26.99 25.28 39.04* -3.53 -1.98 41.46* 40.67* 42.13* -4.89
Mitotic Spindle -4.23 0.07 -4.19 1.85 -3.65 -1.31 -0.97 -1.40 -0.81 -0.32 -3.41 -0.53 -4.24 -1.65 -2.89
None 2.31 -3.24 -0.27 -7.10 3.55 4.07 3.32 -0.44 -3.71 -3.13 3.74 -0.61 1.28 -3.38 1.86
Nuclear Periphery 1.44 -7.01 -7.94 -12.90 3.01 2.28 -3.08 2.94 1.88 11.19 2.35 -6.55 -3.20 -9.92 5.26
Nuclear Periphery Foci -0.47 -5.58 -5.51 -4.95 2.21 2.83 -7.65 -3.49 -4.81 4.17 1.49 -7.45 -5.71 -6.11 3.02
Nucleolus 2.69 -5.58 1.98 -2.02 6.80 4.63 5.30 5.73 1.75 0.72 3.96 -1.35 6.68 3.37 6.90
Nucleus 2.83 -35.18 -35.13 -36.27 16.30 11.25 -19.60 1.94 -6.75 19.99 10.23 -38.04 -27.69 -33.28 21.06
Peroxisomes -3.70 13.20 11.39 8.85 -2.58 -10.09 3.33 -4.04 1.92 -5.64 -7.69 12.11 6.79 13.06 -4.86
Vacuole -3.88 -11.26 -30.67 -20.09 -15.52 1.19 -11.93 -9.57 -10.06 4.60 -2.20 -15.47 -30.40 -28.74 -9.19
Vacuole Periphery 1.70 8.95 7.99 5.61 -0.77 2.25 13.16 12.18 11.76 -1.03 1.89 15.36 13.38 11.24 -1.32
Standard name
Human Ortholog
Description Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex
Localization
Cell Percentages mitochondrion (67%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Qri7

Qri7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qri7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available