Standard name
Human Ortholog
Description Protein of unknown function; deletion confers sensitivity to cationic compounds; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.07 – 0.11 0 0 0 0 0 0 0 0 0.07 0.15 0.13 0.06 0.09 0.18 0 0 0.05 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.21 0.21 – 0.35 0.12 0.06 0.07 0.05 0.07 0 0.06 0.09 0.34 0.61 0.72 0.08 0.09 0.13 0.21 0.06 0.07 0.06 0.17 0.12
Cytoplasm 0.21 0.48 – 0.31 0.23 0.35 0.39 0.36 0.4 0.43 0.49 0.51 0.29 0.2 0.2 0.28 0.27 0.32 0.06 0.17 0.09 0 0 0
Endoplasmic Reticulum 0.61 0.31 – 0.42 0.5 0.33 0.21 0.15 0.1 0.09 0.09 0.06 0.42 0.25 0.14 0 0 0 0.34 0.33 0.29 0.3 0.36 0.43
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.27 0.2 0.17 0 0 0 0 0 0
Mitochondria 0 0.07 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0
Nucleus 0 0 – 0 0.06 0.1 0.14 0.16 0.1 0.07 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0.05 0.18 0.25 0.32 0.42 0.43 0.44 0.37 0.44 0.06 0 0 0.33 0.32 0.22 0.32 0.35 0.4 0.56 0.39 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 – 21 5 5 10 3 2 5 5 2 24 72 76 11 13 29 3 1 2 6 9 11
Bud 0 2 – 7 0 2 4 0 5 5 3 6 11 2 4 5 3 4 0 2 0 1 3 3
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 5 6
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 14 – 64 30 16 32 18 20 9 18 29 113 300 404 16 13 21 18 6 4 17 84 69
Cytoplasm 12 32 – 57 58 87 185 133 113 134 148 168 97 97 112 56 37 50 4 17 5 5 16 10
Endoplasmic Reticulum 35 21 – 76 127 84 102 57 28 29 27 19 138 121 77 6 2 6 28 34 15 87 185 246
Endosome 0 0 – 0 0 0 1 0 0 2 0 1 0 0 0 1 1 0 0 0 0 0 0 3
Golgi 1 0 – 0 9 5 3 2 5 4 1 4 8 25 30 53 28 27 0 0 0 0 1 5
Mitochondria 0 5 – 0 0 0 0 0 2 2 3 3 1 0 3 5 7 4 0 1 2 0 2 5
Nucleus 0 0 – 4 14 24 69 60 28 22 12 15 3 1 1 10 6 4 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Nucleolus 0 0 – 0 0 0 1 0 1 0 1 0 0 0 0 4 3 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 – 3 0 0 0 0 0 1 0 0 11 15 23 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 – 9 46 64 154 157 120 139 113 147 20 9 5 65 44 35 27 36 22 164 197 206
Unique Cell Count 57 67 183 254 251 480 373 281 314 302 332 330 489 565 197 137 157 85 104 55 292 509 574
Labelled Cell Count 63 81 241 289 287 561 430 324 352 331 394 426 642 735 234 158 181 85 104 55 292 509 574


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.8 13.8 – 20.0 26.0 28.7 29.1 30.1 30.3 27.6 29.4 28.5 17.1 15.4 14.3 32.4 34.3 35.9 17.8 16.8 17.8
Std Deviation (1e-4) 3.4 5.0 – 4.4 6.5 7.7 8.5 8.7 8.9 8.9 8.9 8.5 3.5 2.7 2.6 10.5 11.4 10.0 4.0 4.1 3.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4705 5.6841 4.3293 4.2054 3.6806 4.9357 4.2166 6.361 5.2496 4.579 4.9004 6.0899 4.7005 5.8943 5.2148 4.5846 4.466 5.279
Actin 0.0102 0.0022 0.007 0.0046 0.0033 0.0037 0.0054 0.0012 0.0182 0.0147 0.0007 0.0016 0.0288 0.0029 0.0203 0.0242 0.022 0.0102
Bud 0.0012 0.0019 0.0049 0.0025 0.0029 0.0016 0.0007 0.0004 0.0019 0.0045 0.0028 0.0005 0.0015 0.001 0.0016 0.0015 0.0018 0.0012
Bud Neck 0.0105 0.005 0.0144 0.0224 0.0076 0.0144 0.0063 0.0081 0.0187 0.0073 0.0069 0.0059 0.0044 0.0051 0.012 0.0294 0.0055 0.0143
Bud Periphery 0.0067 0.0089 0.0268 0.0191 0.0206 0.0153 0.0048 0.0025 0.0115 0.0086 0.0217 0.0066 0.0096 0.0059 0.0126 0.0115 0.0078 0.0083
Bud Site 0.0086 0.0566 0.0346 0.0283 0.0083 0.0032 0.0113 0.0434 0.0166 0.0299 0.0042 0.0013 0.0105 0.0282 0.0137 0.0207 0.0073 0.0024
Cell Periphery 0.3734 0.3209 0.3527 0.329 0.3355 0.3781 0.4469 0.4364 0.3686 0.2659 0.3283 0.4926 0.4102 0.3688 0.414 0.3145 0.3029 0.3504
Cytoplasm 0.1858 0.2473 0.168 0.1825 0.1445 0.1878 0.1634 0.1672 0.1265 0.14 0.2228 0.0689 0.1474 0.1727 0.0998 0.1053 0.1174 0.143
Cytoplasmic Foci 0.0101 0.0083 0.016 0.031 0.0144 0.0045 0.0149 0.0051 0.0096 0.0696 0.0033 0.0006 0.006 0.0071 0.0052 0.0015 0.0268 0.0027
Eisosomes 0.0035 0.0005 0.0006 0.0002 0.0015 0.0002 0.0012 0.0003 0.0006 0.0001 0 0.0009 0.0028 0.0005 0.0003 0.0002 0.001 0.0004
Endoplasmic Reticulum 0.0388 0.0342 0.04 0.0448 0.0223 0.0295 0.0218 0.0135 0.0352 0.0463 0.0075 0.0128 0.032 0.0392 0.039 0.0105 0.0131 0.0261
Endosome 0.0312 0.0139 0.0264 0.0437 0.0584 0.012 0.0185 0.008 0.0175 0.0336 0.0061 0.0009 0.012 0.014 0.0116 0.0044 0.0349 0.007
Golgi 0.009 0.0027 0.0078 0.0133 0.0065 0.0023 0.0078 0.0012 0.0183 0.0215 0.0004 0.0003 0.0047 0.0022 0.003 0.0005 0.0078 0.0018
Lipid Particles 0.0399 0.0594 0.0389 0.0273 0.04 0.0395 0.0392 0.0622 0.0544 0.028 0.0208 0.0243 0.0556 0.077 0.0488 0.0213 0.028 0.0323
Mitochondria 0.0074 0.0005 0.0108 0.0048 0.002 0.0013 0.0146 0.0001 0.0075 0.0037 0.0001 0 0.003 0.0001 0.0008 0.0002 0.0023 0.0009
None 0.0026 0.0015 0.0092 0.0016 0.0336 0.0054 0.0092 0.0018 0.0027 0.0009 0.0005 0.0003 0.0046 0.0007 0.0016 0.0003 0.0196 0.001
Nuclear Periphery 0.004 0.0015 0.0021 0.002 0.0044 0.0014 0.0023 0.001 0.002 0.002 0.0008 0.0007 0.003 0.0017 0.0022 0.0013 0.0037 0.0019
Nucleolus 0.0012 0.0014 0.0014 0.0009 0.0017 0.0006 0.0007 0.0009 0.0023 0.0009 0.0006 0.0004 0.0012 0.0009 0.0009 0.0013 0.0009 0.0006
Nucleus 0.0054 0.0022 0.0033 0.0024 0.0029 0.002 0.0013 0.0012 0.0021 0.0023 0.0011 0.0007 0.0032 0.0014 0.0015 0.0021 0.0016 0.0013
Peroxisomes 0.0028 0.0008 0.0081 0.0123 0.0009 0.0003 0.0018 0.0001 0.0052 0.0175 0 0 0.0005 0.0001 0.0038 0.0001 0.0022 0.0004
Punctate Nuclear 0.0004 0.0003 0.0017 0.0008 0.0015 0.0002 0.0014 0.0002 0.0008 0.0014 0.0001 0 0.0013 0.0002 0.0003 0.0001 0.0022 0.0002
Vacuole 0.2437 0.2284 0.2228 0.2239 0.2851 0.2945 0.2242 0.2447 0.2783 0.2994 0.3705 0.3797 0.2558 0.2693 0.3049 0.4469 0.3886 0.392
Vacuole Periphery 0.0036 0.0014 0.0025 0.0024 0.0024 0.0021 0.0024 0.0005 0.0016 0.002 0.0009 0.0008 0.0019 0.001 0.002 0.0024 0.0026 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.6962 19.0298 21.9659 20.3469 13.8001 21.2758 24.8529 23.0869 24.6672 25.2758
Translational Efficiency 0.9077 0.8722 0.6737 0.835 1.1498 0.9595 0.7232 0.6376 0.882 0.6503

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1346 424 2398 1677 1792 984 116 1155 3138 1408 2514 2832

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1870.21 1923.12 1180.92 1070.77 1827.77 1441.77 1298.65 1029.24 1845.97 1586.72 1186.35 1053.83
Standard Deviation 363.58 415.09 505.94 150.27 377.03 280.43 160.24 162.88 371.92 394.47 495.94 156.87
Intensity Change Log 2 — 0.040249 -0.663289 -0.804552 — -0.342244 -0.493072 -0.828505 — -0.136178 -0.576647 -0.816341

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000158 0.000478 0.009002 0.037352 0.000088 0.000690 0.044198 0.050624 0.000118 0.000626 0.010626 0.042765
Bud Neck 0.000977 0.003086 0.013051 0.023216 0.002468 0.003358 0.059181 0.034835 0.001829 0.003276 0.015179 0.027955
Bud Site 0.002282 0.008128 0.037851 0.098607 0.003137 0.009565 0.137791* 0.133903* 0.002770 0.009132 0.042462 0.113002*
Cell Periphery 0.573230* 0.630487* 0.723030* 0.498185* 0.544066* 0.640603* 0.522525* 0.270131* 0.556575* 0.637557* 0.713778* 0.405176*
Cytoplasm 0.003991 0.002796 0.164715* 0.254341* 0.009204 0.016623 0.086265 0.345951* 0.006968 0.012459 0.161095* 0.291703*
Cytoplasmic Foci 0.000041 0.001033 0.001352 0.004773 0.000604 0.000938 0.005343 0.016139 0.000363 0.000967 0.001536 0.009409
Eisosomes 0.000184 0.000213 0.004948 0.011784 0.000111 0.000221 0.013323 0.016598 0.000142 0.000219 0.005335 0.013747
Endoplasmic Reticulum 0.019113 0.013838 0.010879 0.007398 0.013511 0.016563 0.032506 0.004769 0.015914 0.015743 0.011877 0.006326
Endosome 0.000456 0.001479 0.000868 0.003565 0.001138 0.002433 0.001701 0.005311 0.000845 0.002146 0.000906 0.004277
Golgi 0.000370 0.000828 0.000762 0.005541 0.000647 0.000690 0.001646 0.002374 0.000528 0.000732 0.000802 0.004250
Lipid Particles 0.001806 0.002643 0.005107 0.003048 0.002910 0.004029 0.005134 0.006888 0.002436 0.003611 0.005109 0.004614
Mitochondria 0.000041 0.004345 0.000672 0.002915 0.000057 0.001408 0.000057 0.001422 0.000050 0.002292 0.000643 0.002306
Mitotic Spindle 0.000160 0.000339 0.000479 0.003569 0.000099 0.000196 0.000468 0.001259 0.000126 0.000239 0.000479 0.002627
None 0.000003 0.000025 0.000724 0.000051 0.000012 0.000295 0.000013 0.000345 0.000008 0.000214 0.000691 0.000171
Nuclear Periphery 0.001853 0.000945 0.000925 0.000242 0.001035 0.001202 0.000233 0.000074 0.001386 0.001124 0.000893 0.000174
Nuclear Periphery Foci 0.000600 0.000577 0.000512 0.000904 0.000453 0.000667 0.002459 0.000866 0.000516 0.000640 0.000602 0.000888
Nucleolus 0.000029 0.000137 0.000060 0.000041 0.000057 0.000037 0.000010 0.000040 0.000045 0.000067 0.000057 0.000041
Nucleus 0.000095 0.000415 0.002858 0.004209 0.000247 0.000216 0.003298 0.009209 0.000182 0.000276 0.002878 0.006248
Peroxisomes 0.000255 0.000611 0.003382 0.001223 0.000442 0.001778 0.000689 0.003124 0.000362 0.001427 0.003257 0.001998
Vacuole 0.393559* 0.326283* 0.018435 0.036349 0.418426* 0.297110* 0.083038 0.095372 0.407759* 0.305895* 0.021416 0.060421
Vacuole Periphery 0.000797 0.001314 0.000390 0.002687 0.001288 0.001380 0.000120 0.000765 0.001077 0.001360 0.000377 0.001903

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.16 -11.83 -17.38 -17.19 -12.53 -3.14 -6.18 -16.83 -16.59 -0.76 -3.60 -13.17 -24.15 -23.79 -16.54
Bud Neck -2.98 -14.84 -18.12 -14.29 -6.91 -1.17 -5.71 -16.36 -15.59 2.43 -2.68 -14.06 -23.66 -21.06 -8.95
Bud Site -1.96 -15.95 -23.74 -18.12 -13.35 -3.04 -8.43 -23.47 -21.15 0.38 -3.72 -17.40 -33.13 -28.20 -17.64
Cell Periphery -4.43 -18.06 7.47 9.52 22.37* -10.32 1.05 25.81* 32.03* 8.24 -10.83 -22.37 19.84 26.15* 36.08*
Cytoplasm 1.07 -36.83 -41.56 -42.10 -12.29 -2.77 -8.09 -43.77 -42.04 -21.38 -2.99 -36.72 -58.79 -56.12 -20.81
Cytoplasmic Foci -1.49 -5.62 -8.08 -4.30 -5.34 -0.83 -2.88 -11.18 -10.79 -4.46 -1.84 -4.62 -13.35 -11.62 -11.00
Eisosomes -0.49 -15.84 -15.48 -15.41 -8.45 -3.63 -6.76 -14.22 -14.13 -1.36 -2.75 -17.19 -20.96 -20.83 -11.65
Endoplasmic Reticulum 1.62 3.85 5.51 2.27 2.51 -1.40 -2.61 6.92 5.86 3.87 0.10 2.83 7.99 5.62 4.67
Endosome -1.45 -4.75 -5.73 -2.35 -4.93 -1.85 -1.26 -5.29 -2.91 -3.63 -2.47 -0.44 -7.39 -3.19 -7.45
Golgi -0.92 -1.36 -4.29 -3.61 -3.86 -0.17 -3.28 -2.66 -2.58 -1.06 -0.95 -0.95 -4.86 -4.48 -4.27
Lipid Particles -0.74 -4.23 -1.93 -0.38 2.82 -0.89 -1.87 -3.57 -1.89 -1.06 -1.26 -3.95 -3.65 -1.03 0.68
Mitochondria -1.49 -10.00 -3.78 0.48 -2.94 -1.97 0.05 -2.30 0.02 -2.30 -2.26 -9.85 -4.41 0.01 -3.22
Mitotic Spindle -1.18 -12.64 -3.26 -3.05 -2.96 -4.61 -1.56 -1.95 -1.79 -1.06 -2.32 -13.39 -3.77 -3.59 -3.23
None -1.54 -1.87 -12.01 -1.66 1.75 -0.99 -0.06 -1.68 -0.10 -1.68 -1.03 -1.86 -2.02 0.23 1.39
Nuclear Periphery 5.49 6.88 12.24 6.25 11.47 -1.29 8.87 11.85 11.07 3.78 2.48 5.85 16.21 11.76 14.36
Nuclear Periphery Foci 0.10 1.43 -1.25 -1.12 -1.66 -2.86 -4.30 -3.34 -1.81 3.15 -1.63 -1.95 -2.61 -1.71 -2.05
Nucleolus -1.27 -6.68 -1.52 1.12 2.20 2.62 7.87 2.01 -0.72 -6.21 -0.86 -2.62 0.54 0.97 2.65
Nucleus -0.97 -6.69 -9.16 -6.83 -2.23 0.61 -5.08 -11.29 -11.41 -6.29 -0.67 -6.74 -14.33 -13.86 -6.01
Peroxisomes -1.40 -14.47 -9.97 -2.32 9.67 -3.39 -1.70 -6.46 -2.33 -5.57 -3.74 -14.06 -9.07 -1.68 4.87
Vacuole 5.46 61.31* 54.80* 26.24 -8.57 13.68 22.12 39.93* 21.57 -0.94 14.29 91.78* 69.68* 37.38 -15.52
Vacuole Periphery -1.72 4.48 -2.98 -1.98 -3.67 -0.22 7.06 1.40 1.27 -1.78 -1.00 6.78 -1.98 -1.11 -3.82
Standard name
Human Ortholog
Description Protein of unknown function; deletion confers sensitivity to cationic compounds; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane
Localization
Cell Percentages cell periphery (24%), ER (22%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ilt1

Ilt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ilt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available