Standard name
Human Ortholog
Description Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.73 0.59 0.3 0.38 0.25 0.29 0.4 0.39 0.39 0.14 0.4 0.47 0.43 0.49 0.47 0.62 0.61 0.78 0.72 0.34 0.52 0.25 0.16
Bud 0.23 0.24 0.13 0.19 0.13 0.21 0.14 0.24 0.18 0.19 0.15 0.15 0.16 0.18 0.14 0.14 0.17 0 0.08 0 0.07 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0
Bud Site 0.08 0 0 0 0 0 0.05 0 0.09 0.14 0 0.06 0 0 0 0 0
Cell Periphery 0 0.09 0 0 0.06 0 0 0.09 0 0 0.07 0.06 0.16 0.16 0.09 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0.16 0.18 0.43 0.4 0.49 0.38 0.3 0.18 0.15 0.14 0.16 0.41 0.42 0.28 0.36 0.29 0.17 0 0.05 0.09 0.15 0.54 0.58
Endoplasmic Reticulum 0.06 0.1 0.13 0.08 0.13 0.06 0.16 0.05 0.07 0.19 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.09 0.06 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0.05 0.08 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.1 0 0 0.07 0.06 0.14 0.1 0.33 0 0 0 0 0 0
Mitochondria 0.18 0.3 0.13 0.13 0.23 0.28 0.36 0.45 0.56 0.48 0.53 0 0 0 0.08 0.1 0.22 0 0 0.25 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0 0.17 0.09 0 0.13 0.14 0.07 0.13 0.19 0.12 0 0.07 0.14 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 115 54 7 32 18 34 54 40 33 3 36 55 94 116 40 13 11 149 80 12 61 47 33
Bud 36 22 3 16 9 25 19 24 15 4 14 17 34 42 12 3 3 7 9 1 8 4 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 2 4 3 5
Bud Site 13 4 0 1 0 0 7 2 8 3 3 7 0 1 0 0 0
Cell Periphery 7 8 1 3 4 3 3 9 3 0 6 7 35 39 8 2 2 1 0 0 0 3 3
Cytoplasm 26 16 10 34 35 45 40 18 13 3 15 47 93 66 31 6 3 5 5 3 17 101 120
Endoplasmic Reticulum 9 9 3 7 9 7 21 5 6 4 4 4 16 12 2 0 0 1 0 0 3 3 6
Endosome 4 0 2 5 1 2 6 2 1 0 0 2 3 18 5 0 0 5 5 2 6 6 5
Golgi 1 4 0 1 1 2 6 1 1 2 3 4 16 15 12 2 6 3 0 0 1 0 1
Mitochondria 28 27 3 11 16 33 49 46 48 10 48 5 1 10 7 2 4 5 0 9 1 7 4
Nucleus 1 0 0 0 2 3 3 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 2 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 2 0 0 0 1 1 1 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 26 4 4 8 1 15 19 7 11 4 11 5 15 34 3 0 0 4 1 0 1 0 2
SpindlePole 0 0 0 1 0 3 6 2 3 0 2 1 1 9 4 0 0 2 0 0 1 0 0
Vac/Vac Membrane 1 0 0 1 1 2 1 1 1 0 1 3 0 2 0 0 0 0 2 2 3 8 8
Unique Cell Count 158 91 23 85 71 119 135 102 85 21 91 116 219 239 86 21 18 192 113 37 118 190 208
Labelled Cell Count 268 151 33 120 98 177 236 159 143 33 144 159 309 368 125 28 29 192 113 37 118 190 208


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 10.4 7.5 7.8 7.5 7.1 6.9 7.1 7.0 6.4 6.9 11.1 11.6 11.5 13.5 15.2 16.3 10.0 10.9 12.5
Std Deviation (1e-4) 1.5 1.9 1.0 1.8 1.8 2.0 1.9 1.7 1.9 1.6 2.4 2.2 1.8 2.4 3.7 3.9 2.8 2.2 2.2 2.5
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8059 10.2482 9.6954 8.8982 9.4239 10.0235 6.1774 6.5023 6.199 6.1329 5.2026 6.9984 9.2661 10.7398 9.6769 9.2693 8.8923 10.0893
Actin 0.7032 0.5346 0.7163 0.7432 0.7455 0.8083 0.6944 0.4626 0.7246 0.7838 0.6812 0.8037 0.8025 0.745 0.8172 0.8925 0.8482 0.8692
Bud 0.0027 0.0048 0.0347 0.0188 0.006 0.0021 0.0009 0.0033 0.048 0.003 0.01 0.0004 0.0026 0.0033 0.0068 0.0045 0.0085 0.0005
Bud Neck 0.0103 0.0089 0.0118 0.0023 0.0214 0.0881 0.0516 0.0062 0.017 0.001 0.0015 0.0527 0.0059 0.0056 0.0087 0.0052 0.0004 0.0754
Bud Periphery 0.0042 0.0024 0.0372 0.0225 0.0042 0.0032 0.0008 0.0022 0.0576 0.0026 0.0227 0.0003 0.0023 0.0023 0.0063 0.0073 0.0307 0.0005
Bud Site 0.0204 0.1488 0.0361 0.0157 0.0042 0.0041 0.0521 0.1754 0.0354 0.0018 0.0029 0.0009 0.0188 0.0687 0.0248 0.0035 0.0021 0.0007
Cell Periphery 0.0046 0.0017 0.0008 0.0012 0.0003 0.0003 0.0016 0.0036 0.0014 0.0002 0.0005 0.0002 0.0005 0.0004 0.0007 0.0003 0.0005 0.0002
Cytoplasm 0.0574 0.1903 0.0678 0.0652 0.0774 0.03 0.014 0.3053 0.0672 0.1861 0.0271 0.0728 0.0202 0.082 0.0746 0.014 0.0397 0.0075
Cytoplasmic Foci 0.0478 0.0515 0.0334 0.0179 0.0534 0.0276 0.0903 0.0109 0.0216 0.0053 0.1143 0.0524 0.0378 0.0475 0.0171 0.0143 0.0039 0.0147
Eisosomes 0.0007 0.0002 0.0005 0.0004 0.0006 0.0004 0.0013 0.002 0.0012 0.0002 0.0002 0.0004 0.0006 0.0002 0.0003 0.0007 0.0003 0.0003
Endoplasmic Reticulum 0.0103 0.0009 0.0013 0.0045 0.0037 0.001 0.0012 0.0026 0.0006 0.0025 0.0238 0.0008 0.0015 0.0003 0.0023 0.0005 0.0155 0.002
Endosome 0.0274 0.0027 0.0084 0.0235 0.0203 0.0095 0.0053 0.003 0.0019 0.0023 0.0143 0.0017 0.0182 0.0055 0.0062 0.007 0.0089 0.0034
Golgi 0.0222 0.0088 0.0163 0.0101 0.02 0.0126 0.0048 0.0154 0.0072 0.0055 0.0151 0.0053 0.0242 0.0071 0.0055 0.0141 0.0061 0.0058
Lipid Particles 0.0118 0.0006 0.0023 0.0017 0.0113 0.001 0.0039 0.0023 0.0007 0.0005 0.0055 0.0006 0.0127 0.0003 0.0076 0.0069 0.0011 0.0015
Mitochondria 0.007 0.0007 0.0011 0.0084 0.0027 0.0009 0.0013 0.0012 0.0043 0.0016 0.0017 0.0003 0.0135 0.0003 0.0007 0.0197 0.0191 0.006
None 0.0047 0.0004 0.0026 0.0014 0.001 0.0004 0.0357 0.0006 0.0003 0.0002 0.0012 0.0001 0.0015 0.0003 0.0033 0.0004 0.0015 0.0003
Nuclear Periphery 0.0168 0.0002 0.0053 0.0104 0.0019 0.0002 0.0008 0.0002 0.0001 0.0001 0.0016 0 0.0018 0.0001 0.0003 0.0002 0.0054 0.0006
Nucleolus 0.0006 0 0.0011 0.0004 0.0001 0.0001 0.003 0.0001 0 0 0 0 0.0003 0 0.0001 0.0001 0.0001 0
Nucleus 0.0039 0.0003 0.0025 0.0039 0.0011 0.0004 0.003 0.0002 0.0001 0.0001 0.0003 0 0.0005 0.0001 0.0003 0.0001 0.0009 0.0003
Peroxisomes 0.0311 0.0415 0.0162 0.031 0.0219 0.0091 0.0075 0.002 0.0103 0.0025 0.0711 0.0069 0.0294 0.0304 0.0162 0.0075 0.0039 0.0105
Punctate Nuclear 0.0075 0.0005 0.0036 0.0013 0.0024 0.0003 0.0263 0.0003 0.0003 0.0001 0.0046 0.0004 0.0038 0.0003 0.0007 0.0004 0.0008 0.0003
Vacuole 0.0034 0.0002 0.0004 0.0103 0.0004 0.0003 0.0003 0.0003 0.0002 0.0005 0.0003 0 0.0009 0.0002 0.0002 0.0003 0.0016 0.0001
Vacuole Periphery 0.0022 0 0.0002 0.0059 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0002 0 0.0005 0 0.0001 0.0003 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 138.5919 106.8744 133.8595 121.4682 150.5648 143.8426 106.512 108.4714 146.2208 119.3087
Translational Efficiency 1.375 1.4103 1.1545 1.4564 1.1739 1.2391 1.2677 1.5371 1.2212 1.3014

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1876 1222 257 1399 1609 1464 337 953 3485 2686 594 2352

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 967.66 966.48 1640.08 1446.98 983.28 1093.94 1561.69 1565.07 974.87 1035.95 1595.61 1494.83
Standard Deviation 134.64 187.03 212.76 246.69 145.91 160.47 248.26 280.71 140.17 184.33 236.77 267.37
Intensity Change Log 2 -0.001760 0.761194 0.580473 0.153859 0.667434 0.670553 0.078769 0.714700 0.626576

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.555874* 0.613551* 0.607849* 0.667299* 0.747096* 0.683620* 0.632229* 0.672110* 0.644160* 0.651742* 0.621681* 0.669248*
Bud Neck 0.010940 0.005084 0.026354 0.003856 0.003572 0.004277 0.010462 0.006669 0.007538 0.004644 0.017338 0.004996
Bud Site 0.232326* 0.071187 0.227868* 0.160797* 0.103248* 0.133491* 0.127185* 0.212835* 0.172732* 0.105146* 0.170747* 0.181882*
Cell Periphery 0.001921 0.000562 0.006026 0.001112 0.001696 0.001341 0.001008 0.005567 0.001817 0.000987 0.003179 0.002917
Cytoplasm 0.039162 0.025767 0.030121 0.038198 0.018731 0.038151 0.013757 0.030253 0.029729 0.032517 0.020837 0.034979
Cytoplasmic Foci 0.058367 0.061285 0.019735 0.024037 0.026456 0.034233 0.052195 0.011229 0.043634 0.046540 0.038150 0.018847
Eisosomes 0.000801 0.000458 0.001600 0.001298 0.000734 0.000709 0.002057 0.012123 0.000770 0.000595 0.001859 0.005684
Endoplasmic Reticulum 0.000422 0.000309 0.004444 0.000710 0.000518 0.000730 0.001725 0.002018 0.000466 0.000539 0.002901 0.001240
Endosome 0.003430 0.035125 0.019059 0.022811 0.009440 0.006652 0.049246 0.010659 0.006205 0.019606 0.036185 0.017887
Golgi 0.006649 0.025288 0.011818 0.013979 0.012190 0.014055 0.014057 0.008466 0.009207 0.019165 0.013088 0.011745
Lipid Particles 0.001139 0.021856 0.020897 0.009811 0.006388 0.001371 0.036201 0.006442 0.003563 0.010690 0.029580 0.008446
Mitochondria 0.001378 0.000892 0.000249 0.003021 0.001139 0.001886 0.000129 0.001867 0.001268 0.001433 0.000181 0.002553
Mitotic Spindle 0.001047 0.000965 0.001936 0.009284 0.000519 0.002908 0.001998 0.001676 0.000803 0.002024 0.001971 0.006201
None 0.000130 0.000052 0.000025 0.000037 0.000054 0.000033 0.000019 0.000146 0.000095 0.000041 0.000021 0.000081
Nuclear Periphery 0.000002 0.000004 0.000041 0.000091 0.000002 0.000165 0.000023 0.000040 0.000002 0.000092 0.000031 0.000070
Nuclear Periphery Foci 0.000035 0.000126 0.001090 0.000641 0.000046 0.000551 0.000857 0.000316 0.000040 0.000358 0.000958 0.000509
Nucleolus 0.000040 0.000018 0.000007 0.000022 0.000007 0.000032 0.000006 0.000008 0.000025 0.000025 0.000007 0.000016
Nucleus 0.000321 0.000109 0.000857 0.000087 0.000078 0.000234 0.000139 0.000099 0.000209 0.000177 0.000449 0.000092
Peroxisomes 0.084800 0.135934* 0.010714 0.036550 0.067412 0.073621 0.041901 0.014024 0.076772 0.101970* 0.028408 0.027423
Vacuole 0.000939 0.001289 0.009116 0.005044 0.000604 0.001266 0.014677 0.002531 0.000784 0.001277 0.012271 0.004026
Vacuole Periphery 0.000278 0.000138 0.000194 0.001317 0.000069 0.000675 0.000129 0.000923 0.000182 0.000431 0.000157 0.001157

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.13 -1.35 -7.76 -3.22 -2.91 5.12 5.68 5.49 1.05 -1.96 -0.73 2.30 -1.86 -1.11 -3.30
Bud Neck 4.82 -4.99 6.87 1.49 7.67 -0.98 -3.66 -3.55 -2.63 1.95 4.01 -5.56 3.75 -0.68 7.24
Bud Site 18.98* 0.25 6.92 -10.90 3.77 -3.63 -1.98 -9.97 -7.03 -5.42 10.85 0.04 -1.51 -11.18 -1.02
Cell Periphery 8.61 -2.38 4.48 -3.88 2.87 0.76 1.60 -3.88 -4.37 -4.79 3.41 -1.71 -2.55 -4.64 0.26
Cytoplasm 3.53 -0.49 -0.61 -3.52 0.21 -5.54 -0.00 -3.64 1.51 -2.43 -1.23 0.04 -2.82 -1.50 -1.57
Cytoplasmic Foci -0.73 8.32 9.08 8.91 -0.89 -2.42 -4.39 5.41 7.44 6.84 -1.10 0.93 10.10 10.69 5.20
Eisosomes 6.05 -1.68 -1.23 -2.12 0.45 0.22 -1.03 -4.66 -4.67 -3.68 2.70 -1.42 -4.80 -4.97 -3.06
Endoplasmic Reticulum 0.83 -2.75 -1.38 -2.42 2.55 -0.51 -1.77 -2.00 -1.57 -0.36 -0.32 -3.28 -2.35 -1.86 2.03
Endosome -11.27 -5.19 -8.81 3.50 -1.07 1.98 -6.23 -0.63 -2.17 5.92 -8.99 -7.71 -7.47 0.85 4.43
Golgi -8.91 -1.93 -4.09 4.46 -0.80 -0.94 -0.60 1.87 2.53 1.89 -7.05 -1.98 -1.84 4.41 0.55
Lipid Particles -7.10 -5.19 -6.63 3.71 2.82 4.21 -4.71 -0.09 -3.34 4.62 -4.89 -6.66 -4.41 1.24 5.27
Mitochondria 1.61 4.97 -1.81 -2.37 -3.17 -1.24 3.96 -0.79 0.03 -1.96 -0.47 6.42 -1.95 -1.57 -3.76
Mitotic Spindle -0.02 -1.65 -5.03 -5.07 -4.09 -2.55 -2.48 -2.82 1.07 0.68 -2.29 -2.83 -5.47 -3.92 -3.67
None 3.15 4.12 3.51 1.11 -0.60 1.73 2.25 -0.81 -1.00 -1.10 3.69 4.66 0.29 -0.85 -1.21
Nuclear Periphery -2.33 -4.96 -1.93 -1.90 -0.80 -1.15 -5.79 -2.88 0.87 -0.96 -1.16 -7.10 -2.45 0.24 -1.13
Nuclear Periphery Foci -2.91 -3.66 -1.66 -1.40 1.72 -1.43 -2.75 -2.12 0.59 1.93 -1.65 -4.47 -2.12 -0.54 2.25
Nucleolus 0.86 0.99 0.80 -0.39 -1.76 -0.87 -0.18 -0.24 0.83 -0.10 0.47 0.98 0.76 0.57 -1.65
Nucleus 1.54 -2.02 1.58 0.14 2.87 -0.90 -1.99 -1.04 0.76 1.60 0.52 -2.03 1.46 0.78 3.06
Peroxisomes -7.15 18.34 10.36 14.65 -7.79 -1.13 4.07 12.76 13.21 4.95 -5.66 12.02 15.29 18.26 0.17
Vacuole -0.63 -4.54 -4.59 -3.98 2.49 -1.42 -3.75 -3.53 -2.06 3.25 -1.38 -5.20 -5.62 -4.50 3.79
Vacuole Periphery 1.33 0.74 -2.95 -3.51 -3.32 -1.66 -1.58 -2.58 -0.58 -2.38 -1.18 0.36 -3.96 -2.35 -4.13
Standard name
Human Ortholog
Description Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin
Localization
Cell Percentages actin (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rvs167

Rvs167


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rvs167-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available