Standard name
Human Ortholog
Description Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.96 0.97 0.95 0.93 0.86 0.86 0.75 0.7 0.7 0.73 0.99 0.99 0.99 0.94 0.93 0.96 0.97 0.92 0.89 0.83 0.86 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.1 0.14 0.31 0.32 0.34 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0.08 0.05 0.11 0.08 0.09 0 0 0 0 0.08 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 3 1 0 0 1 3 0 0 0 0 0 0 0 2 0 0 5 4
Bud 0 3 1 1 0 2 1 2 5 9 7 6 0 0 0 0 0 0 0 3 0 1 9 11
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 5 5 5 9 5 8 7 8 11 15 3 3 1 10 18 13 18 0 0 0 0 0 0
Cytoplasm 239 408 258 221 332 267 294 262 269 247 225 232 286 458 553 285 209 190 229 400 245 186 500 350
Endoplasmic Reticulum 1 2 1 1 1 1 9 1 4 1 3 6 2 5 3 8 6 6 0 1 2 8 16 8
Endosome 0 1 1 1 2 0 0 0 0 0 2 1 0 1 2 1 0 0 1 4 2 1 6 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 2 0 0 3 0
Mitochondria 1 1 3 4 11 21 33 42 112 112 109 83 1 0 0 1 1 1 0 3 8 4 9 6
Nucleus 1 5 1 0 0 4 6 4 1 1 5 5 2 1 0 3 2 3 0 2 1 0 0 1
Nuclear Periphery 0 0 0 0 0 2 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
SpindlePole 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 2 0 2 1
Vac/Vac Membrane 0 8 5 2 16 8 7 23 18 39 26 28 0 1 0 12 17 2 0 7 3 11 13 8
Unique Cell Count 241 419 268 229 351 287 341 306 357 354 320 318 290 464 556 303 225 198 238 434 275 224 583 407
Labelled Cell Count 245 434 275 236 372 310 362 343 418 422 396 368 296 467 568 329 249 221 238 434 275 224 583 407


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 7.3 6.6 6.4 7.2 6.0 6.0 6.2 5.3 5.3 5.3 5.7 7.4 7.9 7.8 9.3 10.1 9.6 6.7 6.8 7.5
Std Deviation (1e-4) 0.8 0.9 1.0 0.6 1.1 1.3 1.2 1.1 1.0 1.1 1.1 1.0 1.1 1.2 1.2 1.3 1.3 1.1 1.5 1.3 1.6
Intensity Change (Log2) -0.05 0.12 -0.13 -0.15 -0.09 -0.33 -0.31 -0.32 -0.22 0.16 0.25 0.23 0.49 0.61 0.54 0.02 0.04 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.6 0 0.4 0.4 0.3 1.0 1.9 0 0 0 0 2.5 2.3 3.6
Cytoplasm 0.1 -1.0 -1.7 -4.2 -4.4 -7.1 -8.4 -8.2 -7.6 1.8 2.2 3.4 -1.2 -1.7 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.7 3.6 4.4 5.6 9.7 9.7 10.1 8.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 0.7 0.2 3.1 2.1 4.4 3.4 3.6 0 0 0 1.5 3.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5186 5.2909 4.286 4.3296 3.837 4.2668 4.0202 4.3165 4.1458 3.4781 3.5813 3.86 2.8071 3.5335 3.0281 3.0654 3.2706 2.9463
Actin 0.0571 0.0003 0.0208 0.0162 0.0688 0.0011 0.0782 0.0017 0.0478 0.0672 0.006 0.0097 0.0323 0.0024 0.0017 0.0391 0.0213 0.002
Bud 0.0004 0.0073 0.0008 0.0009 0.0043 0.0004 0.0023 0.001 0.002 0.0032 0.0023 0.0019 0.0006 0.0009 0.0003 0.001 0.0072 0.0019
Bud Neck 0.001 0.0001 0.0037 0.0006 0.0004 0.0158 0.0117 0.0003 0.0132 0.0017 0.0018 0.0032 0.0007 0.0003 0.0003 0.0036 0.0042 0.0016
Bud Periphery 0.0006 0.0016 0.0009 0.0021 0.0032 0.0006 0.003 0.0007 0.0019 0.0041 0.0027 0.002 0.0006 0.0004 0.0002 0.001 0.0075 0.0015
Bud Site 0.0033 0.0082 0.0161 0.0019 0.0008 0.0011 0.021 0.004 0.012 0.0069 0.0565 0.0003 0.0014 0.0119 0.0099 0.009 0.0185 0.0013
Cell Periphery 0.0006 0.0003 0.0006 0.0008 0.0003 0.0004 0.0008 0.0002 0.0004 0.0003 0.0014 0.0001 0.0002 0.0001 0.0002 0.0002 0.0007 0.0001
Cytoplasm 0.632 0.9083 0.7996 0.7695 0.5585 0.8673 0.5865 0.9482 0.7677 0.7701 0.7531 0.9317 0.6935 0.9257 0.8815 0.6803 0.6713 0.9053
Cytoplasmic Foci 0.0246 0.0034 0.0174 0.0044 0.0172 0.0339 0.0418 0.0093 0.0283 0.0171 0.0303 0.014 0.0199 0.0127 0.0175 0.0344 0.0355 0.0131
Eisosomes 0.0022 0 0.0007 0.0014 0.0005 0 0.0007 0 0.0002 0.0003 0.0002 0 0.0012 0 0 0.0004 0.0001 0
Endoplasmic Reticulum 0.0157 0.0024 0.0073 0.0084 0.0053 0.0036 0.0192 0.0033 0.0052 0.0066 0.0049 0.0052 0.0085 0.0024 0.007 0.0065 0.0037 0.0042
Endosome 0.04 0.0013 0.0121 0.013 0.0375 0.0046 0.0761 0.0043 0.0292 0.0273 0.0534 0.0116 0.0224 0.0069 0.0259 0.0316 0.0767 0.0155
Golgi 0.0102 0.0002 0.0042 0.0052 0.0363 0.0012 0.0229 0.0007 0.0301 0.02 0.0122 0.0053 0.0069 0.0008 0.0061 0.017 0.0357 0.0005
Lipid Particles 0.029 0 0.0053 0.0034 0.0071 0.0119 0.0196 0.0001 0.0043 0.0077 0.0184 0.0003 0.0115 0.0001 0.0034 0.0228 0.0116 0.0002
Mitochondria 0.0088 0.0001 0.0162 0.0879 0.157 0.0017 0.0105 0.0013 0.0235 0.0137 0.0246 0.0009 0.0423 0.0003 0.0012 0.0042 0.0124 0.0004
None 0.1166 0.0642 0.0693 0.0712 0.0855 0.0424 0.0305 0.0196 0.022 0.0312 0.0043 0.0071 0.1231 0.03 0.0326 0.1075 0.0343 0.0429
Nuclear Periphery 0.0208 0.0003 0.0014 0.0049 0.002 0.0003 0.0246 0.0007 0.001 0.006 0.0016 0.0007 0.0031 0.001 0.0032 0.0088 0.0082 0.0034
Nucleolus 0.0037 0 0.001 0.0002 0.0001 0.0001 0.002 0.0001 0.0002 0.0006 0.0012 0.0003 0.0011 0.0001 0.0002 0.0004 0.0031 0.0001
Nucleus 0.0034 0.0008 0.0017 0.0019 0.0014 0.001 0.0098 0.0014 0.001 0.0024 0.0028 0.0018 0.0026 0.0016 0.0022 0.0014 0.0089 0.0021
Peroxisomes 0.0083 0 0.0123 0.0006 0.0047 0.011 0.0079 0.0006 0.0061 0.0061 0.003 0.0007 0.0186 0.0003 0.0018 0.0207 0.0079 0.0002
Punctate Nuclear 0.0156 0.0001 0.0034 0.0006 0.0012 0.0008 0.0182 0.0007 0.0007 0.0021 0.0011 0.0015 0.0059 0.0005 0.0022 0.008 0.0141 0.0013
Vacuole 0.0039 0.001 0.0047 0.0028 0.0064 0.001 0.0085 0.0015 0.0024 0.004 0.0149 0.0014 0.0027 0.0013 0.0021 0.0014 0.015 0.0018
Vacuole Periphery 0.0023 0.0001 0.0006 0.0022 0.0014 0.0002 0.004 0.0003 0.001 0.0013 0.0031 0.0004 0.0008 0.0002 0.0005 0.0009 0.0024 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3121 20.3054 14.7778 29.9827 23.2205 20.0825 26.7493 27.0476 27.8895 32.7683
Translational Efficiency 1.1234 1.205 1.4061 0.7927 0.9604 1.4861 0.8981 0.9686 0.9513 0.8061

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2112 1528 1365 377 1337 2084 939 87 3449 3612 2304 464

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 768.56 906.48 1102.41 1046.22 799.55 827.01 852.12 1065.71 780.57 860.63 1000.40 1049.87
Standard Deviation 92.97 144.50 153.77 149.42 108.22 404.67 129.17 122.26 100.30 323.82 189.56 144.92
Intensity Change Log 2 0.238117 0.520431 0.444956 0.048717 0.091868 0.414555 0.144653 0.317795 0.429535

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000246 0.000290 0.000485 0.004693 0.000113 0.000490 0.000061 0.000211 0.000195 0.000406 0.000313 0.003853
Bud Neck 0.007264 0.004884 0.004074 0.006445 0.003455 0.014844 0.002830 0.009333 0.005787 0.010631 0.003567 0.006986
Bud Site 0.009896 0.014289 0.011306 0.075807 0.005879 0.029481 0.002976 0.042024 0.008339 0.023054 0.007911 0.069472
Cell Periphery 0.000154 0.000102 0.000121 0.000177 0.000111 0.000237 0.000942 0.000063 0.000137 0.000180 0.000456 0.000156
Cytoplasm 0.609701* 0.640610* 0.626180* 0.393164* 0.650360* 0.579435* 0.505030* 0.599268* 0.625462* 0.605314* 0.576805* 0.431809*
Cytoplasmic Foci 0.204349* 0.170947 0.021745 0.054078 0.188749* 0.216083* 0.038625 0.017526 0.198302* 0.196989* 0.028624 0.047225
Eisosomes 0.000096 0.000038 0.000027 0.000033 0.000051 0.000071 0.000624 0.000027 0.000079 0.000057 0.000271 0.000032
Endoplasmic Reticulum 0.002737 0.002174 0.002542 0.002635 0.002487 0.000973 0.001005 0.000715 0.002640 0.001481 0.001915 0.002275
Endosome 0.013271 0.016481 0.005007 0.048807 0.013128 0.016998 0.003871 0.002957 0.013215 0.016779 0.004544 0.040210
Golgi 0.007463 0.004295 0.000725 0.061265 0.004168 0.010161 0.000183 0.000235 0.006186 0.007680 0.000504 0.049822
Lipid Particles 0.004763 0.002995 0.002297 0.001482 0.004094 0.002377 0.003026 0.000712 0.004504 0.002638 0.002594 0.001337
Mitochondria 0.002399 0.001171 0.000373 0.010755 0.001632 0.006931 0.000241 0.000131 0.002102 0.004495 0.000319 0.008763
Mitotic Spindle 0.000787 0.009652 0.015491 0.105943* 0.001316 0.011797 0.001883 0.010177 0.000992 0.010889 0.009945 0.087987
None 0.004476 0.001828 0.005217 0.002792 0.004148 0.005599 0.017452 0.002598 0.004349 0.004004 0.010203 0.002756
Nuclear Periphery 0.000095 0.000313 0.001904 0.002414 0.000160 0.000150 0.000631 0.000400 0.000120 0.000219 0.001385 0.002036
Nuclear Periphery Foci 0.000628 0.002147 0.005300 0.006996 0.001039 0.000395 0.001454 0.002495 0.000788 0.001136 0.003732 0.006152
Nucleolus 0.000613 0.000456 0.000400 0.000227 0.000462 0.000626 0.000742 0.000257 0.000555 0.000554 0.000539 0.000233
Nucleus 0.040293 0.027421 0.176497* 0.040704 0.041328 0.031998 0.304465* 0.050541 0.040694 0.030062 0.228650* 0.042548
Peroxisomes 0.001674 0.001577 0.000583 0.004052 0.000986 0.004215 0.000865 0.001418 0.001407 0.003099 0.000698 0.003558
Vacuole 0.086453 0.095792 0.119371 0.162604 0.074102 0.062149 0.112948 0.258661* 0.081665 0.076381 0.116753 0.180614
Vacuole Periphery 0.002642 0.002536 0.000355 0.014928 0.002232 0.004990 0.000147 0.000249 0.002483 0.003952 0.000270 0.012176

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.36 -2.04 -1.98 -1.94 -1.31 -3.76 1.30 -3.38 2.57 -3.84 -2.62 -1.92 -2.11 -1.61 -1.64
Bud Neck 3.69 3.48 6.09 1.98 1.52 -9.65 1.07 -0.10 5.62 -0.50 -6.37 3.63 4.04 8.51 0.41
Bud Site -2.79 -0.63 -2.83 -1.85 -2.51 -15.04 2.26 -1.02 2.26 -1.38 -12.77 0.49 -3.22 0.47 -3.28
Cell Periphery 4.29 2.16 2.00 -1.37 0.44 -5.48 -6.35 4.45 6.94 6.77 -3.14 -5.77 2.10 4.27 6.29
Cytoplasm -3.43 8.18 15.71 16.87 10.50 7.79 14.56 7.22 5.08 2.19 3.41 15.56 17.93 16.25 9.81
Cytoplasmic Foci 5.84 47.15 39.44 29.00 -1.78 -4.08 30.12 33.27 40.92 7.82 0.42 55.28 48.22 45.61 1.56
Eisosomes 12.26 14.71 12.26 0.55 -2.57 -5.09 -4.78 4.06 8.13 4.97 6.28 -3.63 12.44 7.70 4.80
Endoplasmic Reticulum 0.96 -0.16 2.84 1.81 5.55 3.56 3.50 3.81 -0.10 1.05 3.09 1.51 3.85 0.78 4.29
Endosome -2.61 13.11 2.04 3.29 -2.40 -3.22 10.11 11.67 16.06 2.47 -4.29 16.55 3.23 5.15 -2.31
Golgi 6.01 16.72 -0.42 -1.30 -2.35 -5.94 5.90 5.96 13.42 0.41 -2.49 15.99 -0.38 0.10 -2.36
Lipid Particles 4.58 7.13 8.93 3.86 1.75 3.60 2.68 5.88 3.98 3.65 6.41 6.91 10.09 4.59 3.27
Mitochondria 4.04 7.73 0.61 -0.61 -1.28 -6.02 3.93 4.10 8.39 1.37 -4.62 8.38 0.75 3.21 -1.34
Mitotic Spindle -4.18 -7.67 -5.48 -4.09 -3.07 -6.00 -1.45 -2.24 1.63 -1.53 -7.35 -7.68 -5.59 -3.84 -3.74
None 4.13 0.22 1.77 -2.26 1.91 -1.42 -4.03 1.37 2.66 4.56 0.46 -3.65 2.07 1.48 4.82
Nuclear Periphery -2.27 -19.74 -8.76 -6.75 3.80 0.13 -14.30 -4.36 -4.37 -1.63 -2.32 -22.09 -9.56 -8.61 1.31
Nuclear Periphery Foci -2.76 -10.73 -5.82 -1.83 2.97 7.26 -4.23 -3.56 -4.40 -2.53 -1.49 -11.39 -6.48 -4.96 0.85
Nucleolus 2.13 3.79 6.26 4.09 4.16 -1.84 -2.31 5.53 4.74 5.77 -0.10 1.32 7.92 5.66 6.82
Nucleus 8.33 -31.43 -7.71 -10.16 17.22 3.69 -26.59 -3.54 -4.09 9.69 6.91 -40.28 -8.31 -10.17 21.50
Peroxisomes 0.66 6.91 2.67 2.25 -1.22 -7.29 0.75 2.47 7.68 0.70 -6.27 4.47 2.58 6.58 -0.71
Vacuole -2.96 -14.84 -20.82 -19.88 -14.18 3.71 -11.78 -10.48 -10.86 -7.57 1.74 -18.88 -23.52 -23.80 -16.38
Vacuole Periphery 0.43 13.36 0.45 0.26 -2.58 -3.78 7.01 7.00 7.29 0.02 -3.34 14.27 0.92 2.73 -2.63
Standard name
Human Ortholog
Description Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Psp1

Psp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available