ORF
Human Ortholog
Description Putative protein of unknown function; conserved among S. cerevisiae strains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.96 0.99 0.96 0.91 – 0.75 0.71 0.7 0.7 0.69 1.0 0.98 0.99 0.96 1.0 0.97 0.97 0.94 0.93 0.91 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.05 0 0.21 – 0.28 0.41 0.37 0.48 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.06 0.07 0 0.05 0 0 0 0 0.09 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 1 1 4 0 0 2 – 4 7 6 12 15 1 2 1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 1 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 4 0 – 3 3 3 7 11 3 2 6 2 0 1 0 0 0 0 0 0
Cytoplasm 431 211 115 304 472 340 – 511 346 490 375 498 204 494 499 22 19 68 420 196 119 69 222 276
Endoplasmic Reticulum 1 1 0 0 2 0 – 1 0 7 0 1 1 1 2 0 2 1 0 0 0 0 1 1
Endosome 0 0 0 0 1 1 – 0 0 2 0 0 0 1 0 0 0 0 2 1 1 0 3 4
Golgi 0 1 0 0 1 0 – 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 1 1
Mitochondria 2 1 6 14 14 80 – 192 197 258 257 355 1 1 0 0 0 1 2 0 1 0 2 2
Nucleus 4 2 1 1 2 3 – 15 9 12 8 8 1 3 1 0 0 0 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 – 4 3 5 1 6 0 1 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 – 1 1 0 0 1 0 2 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Vac/Vac Membrane 1 1 0 0 16 5 – 44 35 31 25 16 0 3 0 2 0 1 1 5 2 2 3 2
Unique Cell Count 434 214 120 306 490 375 678 484 701 539 724 205 503 502 23 19 70 435 209 129 77 239 295
Labelled Cell Count 441 219 126 319 513 432 776 601 816 686 912 211 512 509 26 21 74 435 209 129 77 239 295


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.2 4.9 6.2 6.6 5.0 – 4.6 4.1 4.3 4.0 3.9 8.8 9.1 8.7 9.7 9.2 9.3 7.4 9.1 9.5
Std Deviation (1e-4) 1.1 1.2 0.8 1.0 1.6 1.0 – 0.9 0.9 0.9 0.7 0.8 1.2 1.1 1.0 1.3 1.0 1.7 1.0 1.4 1.4
Intensity Change (Log2) – – – 0.36 0.44 0.05 – -0.07 -0.25 -0.19 -0.29 -0.32 0.85 0.91 0.84 0.99 0.92 0.94 0.61 0.9 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 0.3 -1.8 – -5.0 -5.6 -6.0 -6.0 -6.2 2.4 1.6 3.1 0 0.9 0.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 0 4.1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 2.9 3.0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4862 4.7665 4.2953 4.023 3.2445 4.2771 3.1703 4.6041 3.9043 3.3013 3.6081 4.025 5.4168 7.4682 6.4751 6.6569 5.6487 6.3527
Actin 0.0035 0.0003 0.0038 0.0207 0.0015 0.0003 0.0111 0.0003 0.0081 0.0195 0.0043 0.0008 0.0175 0.0003 0.0043 0.0004 0.001 0.0034
Bud 0.0003 0.0001 0.0005 0.0004 0.0001 0.0001 0.0008 0.0001 0.0002 0.0003 0.0026 0.0001 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001
Bud Neck 0.0029 0.0002 0.0005 0.0085 0.0006 0.0007 0.002 0.0001 0.0003 0.0009 0.0006 0.0007 0.0081 0.0001 0.0009 0.0005 0.0005 0.005
Bud Periphery 0.0003 0 0.0005 0.0006 0 0 0.0012 0 0.0003 0.0003 0.0064 0.0001 0.0002 0.0003 0.0001 0 0.0003 0.0001
Bud Site 0.0014 0.0006 0.0007 0.0013 0.0003 0 0.0044 0.0005 0.0007 0.0013 0.0021 0.0001 0.0056 0.0005 0.0055 0.0001 0.0004 0.0003
Cell Periphery 0.0001 0 0.0001 0.0003 0 0 0.0003 0 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0003 0 0.0001 0
Cytoplasm 0.9017 0.9623 0.9463 0.9198 0.9102 0.9661 0.8688 0.9812 0.9316 0.9074 0.7007 0.9211 0.8279 0.9863 0.9422 0.9679 0.8594 0.9467
Cytoplasmic Foci 0.0202 0.006 0.0125 0.0043 0.0286 0.0054 0.0184 0.0051 0.0076 0.0084 0.0345 0.0108 0.0354 0.0015 0.0086 0.014 0.0056 0.0056
Eisosomes 0 0 0.0001 0.0001 0 0 0.0001 0 0 0.0003 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.006 0.0017 0.0026 0.0045 0.0036 0.0017 0.0063 0.0013 0.0016 0.0009 0.0021 0.0038 0.0047 0.0004 0.0015 0.0009 0.0005 0.0017
Endosome 0.0188 0.0025 0.0058 0.0084 0.0073 0.003 0.0163 0.0011 0.0141 0.0052 0.0753 0.0157 0.023 0.0003 0.0037 0.0017 0.0147 0.0045
Golgi 0.0034 0.0002 0.0019 0.0013 0.0014 0.0001 0.007 0.0001 0.0043 0.0103 0.0228 0.0066 0.0056 0 0.0022 0.0002 0.0113 0.0049
Lipid Particles 0.0028 0 0.0005 0.0003 0.0054 0 0.0139 0 0.0024 0.0117 0.0179 0.003 0.0168 0 0.0053 0 0.0464 0.0078
Mitochondria 0.0017 0.0001 0.0011 0.0004 0.0004 0.0004 0.0054 0.0001 0.012 0.0111 0.0509 0.0113 0.0011 0.0001 0.0028 0.0001 0.0341 0.0041
None 0.0195 0.0197 0.0123 0.0162 0.0037 0.0131 0.0172 0.0068 0.0039 0.0022 0.0035 0.0095 0.0262 0.0075 0.0114 0.0061 0.0015 0.0029
Nuclear Periphery 0.0023 0.0008 0.0015 0.0028 0.0029 0.001 0.0033 0.0005 0.0009 0.0006 0.0072 0.0025 0.003 0.0003 0.0008 0.0005 0.0005 0.001
Nucleolus 0.0001 0 0.0001 0.0001 0.0001 0 0.002 0 0.0002 0.0001 0.0007 0 0.0002 0 0.0001 0 0.0006 0.0001
Nucleus 0.0051 0.0032 0.0055 0.0068 0.0065 0.0065 0.0043 0.002 0.0026 0.0026 0.0056 0.0079 0.0045 0.0017 0.0036 0.0045 0.0024 0.0078
Peroxisomes 0.0043 0.0002 0.0016 0.0001 0.0216 0.0002 0.0047 0.0001 0.0023 0.0157 0.0189 0.0032 0.0135 0 0.0046 0.0004 0.0164 0.0022
Punctate Nuclear 0.0029 0.0008 0.0012 0.001 0.0046 0.0007 0.0062 0.0003 0.0004 0.0004 0.0079 0.0012 0.0051 0.0002 0.0015 0.0023 0.0003 0.0008
Vacuole 0.0021 0.0009 0.0007 0.0018 0.0008 0.0005 0.0049 0.0003 0.0041 0.0005 0.0258 0.0009 0.0007 0.0001 0.0003 0.0002 0.0021 0.0004
Vacuole Periphery 0.0004 0.0001 0.0002 0.0004 0.0002 0.0001 0.0014 0 0.0022 0.0003 0.0098 0.0006 0.0002 0 0.0001 0.0001 0.0018 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
129 1028 371 973 1652 292 2025 1083 1781 1320 2396 2056

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 796.97 979.30 1226.05 1133.53 833.18 986.14 1100.54 1140.41 830.56 980.81 1119.97 1137.15
Standard Deviation 99.46 169.54 141.77 152.35 139.20 148.83 169.00 170.67 137.03 165.21 171.21 162.29
Intensity Change Log 2 — 0.297225 0.621420 0.508225 — 0.243164 0.401511 0.452852 — 0.269848 0.513212 0.480189

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000383 0.000347 0.000648 0.002953 0.000379 0.000666 0.000428 0.003859 0.000379 0.000417 0.000462 0.003430
Bud Neck 0.005858 0.004103 0.003408 0.002614 0.002347 0.009998 0.003352 0.006350 0.002601 0.005407 0.003361 0.004582
Bud Site 0.008132 0.015716 0.015242 0.019026 0.004436 0.031812 0.009193 0.044256 0.004703 0.019277 0.010130 0.032316
Cell Periphery 0.000268 0.000082 0.000178 0.000109 0.000068 0.000132 0.000060 0.000132 0.000082 0.000093 0.000078 0.000121
Cytoplasm 0.540730* 0.713003* 0.481250* 0.651696* 0.691775* 0.473499* 0.708951* 0.683645* 0.680835* 0.660021* 0.673694* 0.668525*
Cytoplasmic Foci 0.274150* 0.099693 0.010329 0.017635 0.107304 0.162097 0.006816 0.030373 0.119389 0.113498 0.007360 0.024344
Eisosomes 0.000115 0.000025 0.000029 0.000018 0.000039 0.000072 0.000008 0.000024 0.000045 0.000036 0.000011 0.000021
Endoplasmic Reticulum 0.002880 0.000990 0.003623 0.001380 0.001183 0.001127 0.000535 0.001227 0.001306 0.001020 0.001013 0.001299
Endosome 0.017715 0.008818 0.002178 0.003532 0.006385 0.022493 0.002105 0.011447 0.007206 0.011843 0.002116 0.007701
Golgi 0.002196 0.002764 0.000158 0.012043 0.001398 0.003492 0.000548 0.008282 0.001456 0.002925 0.000488 0.010062
Lipid Particles 0.010035 0.002634 0.001302 0.000806 0.001705 0.001813 0.000350 0.000646 0.002308 0.002452 0.000497 0.000722
Mitochondria 0.008427 0.001790 0.000345 0.009096 0.000913 0.009870 0.000813 0.009064 0.001457 0.003578 0.000741 0.009079
Mitotic Spindle 0.000963 0.005135 0.002361 0.019677 0.000889 0.011950 0.008369 0.023791 0.000894 0.006643 0.007439 0.021844
None 0.001215 0.002820 0.006557 0.003120 0.002501 0.000429 0.006674 0.005890 0.002408 0.002291 0.006656 0.004579
Nuclear Periphery 0.000618 0.000230 0.001263 0.000597 0.000306 0.000278 0.000331 0.000254 0.000329 0.000240 0.000475 0.000416
Nuclear Periphery Foci 0.001320 0.002318 0.003895 0.002334 0.001698 0.001391 0.001710 0.001714 0.001671 0.002113 0.002049 0.002007
Nucleolus 0.005422 0.000339 0.000143 0.000246 0.000985 0.000888 0.000187 0.000321 0.001306 0.000461 0.000181 0.000285
Nucleus 0.056171 0.052633 0.365201* 0.095605 0.089559 0.044139 0.212422* 0.070530 0.087141 0.050754 0.236079* 0.082397
Peroxisomes 0.003928 0.002303 0.000598 0.001286 0.000883 0.007653 0.001248 0.002794 0.001104 0.003486 0.001147 0.002081
Vacuole 0.057876 0.083101 0.101162 0.149139 0.084617 0.214086* 0.035520 0.092159 0.082680 0.112076 0.045684 0.119125
Vacuole Periphery 0.001596 0.001156 0.000130 0.007088 0.000631 0.002117 0.000381 0.003243 0.000701 0.001369 0.000342 0.005063

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.25 -3.06 -0.96 -1.07 -0.07 -1.19 -0.09 -1.87 -1.37 -2.08 -0.20 -0.46 -1.80 -1.98 -1.79
Bud Neck 1.55 1.13 3.15 3.04 4.49 -5.61 -2.19 -0.75 4.57 0.78 -5.16 -2.29 -0.03 4.25 1.85
Bud Site -2.97 -0.47 -0.52 2.92 0.15 -7.65 -2.67 -5.20 3.79 -3.66 -9.03 -2.91 -6.12 3.12 -3.30
Cell Periphery 4.26 0.86 4.10 -0.77 8.77 -5.25 -0.95 -2.41 2.93 -1.89 -1.34 -2.43 -0.80 0.51 1.40
Cytoplasm -6.46 6.89 1.08 15.83 -10.16 13.14 8.28 12.40 -5.28 6.25 2.67 12.23 16.33 11.49 5.22
Cytoplasmic Foci 9.40 14.55 14.27 20.34 -4.19 -5.53 29.85 24.33 16.51 -9.73 1.59 31.73 28.36 24.81 -11.43
Eisosomes 7.14 5.48 7.54 2.40 8.61 -3.35 3.65 2.07 8.81 -9.03 1.27 3.92 3.15 6.77 -5.48
Endoplasmic Reticulum 1.85 -2.13 2.01 1.02 10.75 0.03 2.01 2.21 3.21 0.85 1.16 -0.61 2.55 2.36 6.58
Endosome 2.81 4.62 5.09 7.96 4.89 -6.93 8.02 4.00 8.47 -1.68 -3.97 9.08 7.47 9.93 -1.10
Golgi -0.34 3.85 0.11 0.32 -1.52 -1.50 2.53 -0.34 1.21 -2.29 -1.66 2.93 -0.52 0.90 -2.34
Lipid Particles 2.18 2.40 2.61 1.28 5.01 -0.32 7.49 4.68 3.25 -5.18 -0.02 5.04 4.12 1.61 -4.48
Mitochondria 1.17 1.38 1.15 -0.05 -1.17 -1.93 0.75 -0.87 1.65 -1.23 -1.38 1.56 -0.33 1.05 -1.61
Mitotic Spindle -3.31 -2.28 -5.31 -3.09 -4.51 -2.52 -5.26 -5.23 0.43 -1.54 -4.30 -5.49 -7.55 -2.39 -2.80
None -1.53 -6.37 -6.05 -0.52 2.91 6.20 -9.86 -4.81 -9.48 1.68 0.19 -10.34 -4.79 -2.60 4.80
Nuclear Periphery 1.10 -4.24 -0.93 -13.21 10.90 -0.56 -8.74 -5.77 -5.94 2.85 1.23 -11.52 -8.25 -14.22 4.35
Nuclear Periphery Foci -3.32 -7.62 -4.02 0.11 5.64 0.49 -0.24 -0.63 -1.03 -0.44 -2.02 -2.71 -2.05 0.48 0.94
Nucleolus 1.04 1.06 1.06 2.20 -0.29 0.30 1.44 1.78 1.66 0.86 1.58 1.81 2.01 2.55 0.82
Nucleus 0.14 -25.63 -9.27 -13.50 21.96 9.48 -32.31 -3.16 -11.82 28.61 10.58 -38.12 -5.76 -17.91 34.72
Peroxisomes 1.63 3.50 3.40 4.02 -0.29 -3.28 0.59 0.56 3.34 -0.18 -4.03 1.65 2.75 4.98 0.16
Vacuole -3.88 -9.80 -21.52 -22.06 -8.54 -11.27 1.28 -20.29 -2.37 -21.17 -6.61 -2.85 -29.65 -21.64 -27.56
Vacuole Periphery 1.14 3.56 0.46 -0.25 -1.64 -3.42 3.99 -0.11 2.79 -1.71 -2.56 5.07 -0.69 0.78 -2.12
ORF
Human Ortholog
Description Putative protein of unknown function; conserved among S. cerevisiae strains
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YEL014C

YEL014C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yel014c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available