Standard name
Human Ortholog
Description Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 – 0.94 0.99 0.98 0.94 0.9 0.85 0.77 – 0.65 0.65 0.99 1.0 0.97 0.97 0.97 0.93 0.95 0.89 0.89 0.96 0.91 0.87
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.1 0.09 0 0.22 0.19 0.25 0.46 – 0.52 0.53 0 0 0.05 0 0 0 0 0 0.05 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 1 0 0 1 – 1 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud 1 – 3 2 3 6 5 8 15 – 7 15 0 1 16 0 0 0 0 0 0 0 1 6
Bud Neck 0 – 0 0 0 1 1 1 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 3
Bud Site 0 – 0 0 0 0 0 2 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 1 2 16 5 12 10 4 – 7 11 2 10 11 14 6 11 0 0 0 0 0 0
Cytoplasm 56 – 148 374 542 361 720 884 278 – 170 366 156 469 487 292 166 222 67 35 146 111 264 356
Endoplasmic Reticulum 0 – 1 1 3 0 5 3 0 – 3 5 1 0 2 18 4 5 0 0 1 0 3 9
Endosome 0 – 0 0 0 1 0 5 0 – 0 0 0 0 1 0 0 4 0 0 0 1 1 5
Golgi 0 – 0 1 0 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 5
Mitochondria 2 – 15 35 19 83 150 260 166 – 136 300 0 4 27 7 3 0 0 0 7 0 1 3
Nucleus 0 – 1 0 0 1 2 10 1 – 1 5 1 0 0 3 2 2 0 0 2 0 1 0
Nuclear Periphery 0 – 0 0 0 0 3 1 0 – 3 21 0 0 1 0 0 1 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 1 0 – 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 – 0 0 0 0 1 3 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 4
Vac/Vac Membrane 1 – 1 0 1 2 3 15 4 – 11 41 0 0 1 6 4 7 0 0 2 2 8 5
Unique Cell Count 57 157 379 554 384 797 1041 363 260 564 158 469 503 301 172 238 71 40 165 117 292 412
Labelled Cell Count 60 170 415 584 461 902 1203 470 339 765 160 484 548 341 185 253 71 40 165 117 292 412


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 – 4.3 4.1 5.3 4.4 4.0 3.9 3.5 – 3.8 4.2 6.5 6.4 5.9 9.9 10.2 11.1 6.6 6.4 7.0
Std Deviation (1e-4) 2.1 – 0.7 0.9 1.0 1.1 1.1 0.9 1.1 – 1.4 1.7 1.3 1.7 1.6 2.0 2.7 2.9 1.4 1.6 2.5
Intensity Change (Log2) – – – -0.08 0.29 0.01 -0.12 -0.15 -0.32 – -0.2 -0.05 0.58 0.57 0.45 1.2 1.23 1.36 0.6 0.57 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 2.3 0 0
Cytoplasm 2.9 2.3 -0.1 -1.6 -3.2 -4.8 – -6.7 -7.2 2.2 5.2 1.5 1.4 1.0 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.7 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 -3.2 0 0 0 0 – 0 0 -4.0 0 -1.9 -3.4 -3.1 -4.9
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5723 9.4172 7.7526 8.0878 6.195 8.6574 7.4466 12.298 10.3262 10.5252 9.5923 9.7434 4.3864 7.6114 5.6495 6.4952 4.7566 5.6005
Actin 0.0409 0.0008 0.0083 0.001 0.0003 0.0009 0.0124 0.0001 0.0115 0.0016 0.0003 0.0018 0.0205 0.0004 0.0009 0.0275 0.0149 0.0008
Bud 0.0008 0.0016 0.0039 0.0015 0.0007 0.0006 0.001 0.0002 0.0004 0.0007 0.0001 0.0003 0.0004 0.0112 0.0006 0.0004 0.0006 0.0003
Bud Neck 0.0024 0.0004 0.001 0.0011 0.0031 0.0015 0.0057 0.0002 0.0003 0.0009 0.0002 0.0023 0.001 0.0002 0.0004 0.0004 0.0009 0.0006
Bud Periphery 0.0012 0.0025 0.0039 0.0014 0.0003 0.0008 0.0011 0.0001 0.0003 0.0015 0 0.0003 0.0003 0.0025 0.0003 0.0003 0.0004 0.0002
Bud Site 0.002 0.0165 0.0048 0.0014 0.0016 0.0002 0.0036 0.001 0.0006 0.0025 0 0.0003 0.0017 0.0018 0.0011 0.0003 0.0017 0.0001
Cell Periphery 0.0016 0.0039 0.0009 0.0009 0.0003 0.0009 0.0008 0.0002 0.0002 0.0005 0 0.0001 0.0007 0.0002 0.0002 0.0001 0.0003 0.0001
Cytoplasm 0.7838 0.9232 0.9028 0.8402 0.854 0.9162 0.7576 0.9806 0.9056 0.9135 0.9809 0.921 0.8054 0.9528 0.9214 0.8969 0.8844 0.935
Cytoplasmic Foci 0.0158 0.0056 0.0137 0.0112 0.0519 0.0059 0.03 0.0031 0.0097 0.0101 0.0035 0.0095 0.0231 0.0065 0.0171 0.0126 0.0165 0.0082
Eisosomes 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0009 0 0 0.0003 0.0004 0
Endoplasmic Reticulum 0.0572 0.0147 0.0121 0.0359 0.0348 0.0315 0.0427 0.0062 0.0137 0.0047 0.0022 0.0159 0.0431 0.0034 0.013 0.0064 0.0101 0.0201
Endosome 0.0338 0.0101 0.0159 0.0531 0.0209 0.017 0.0617 0.0024 0.0142 0.0209 0.0083 0.0206 0.0327 0.0116 0.0167 0.0294 0.0207 0.0193
Golgi 0.0096 0.0014 0.0033 0.0014 0.0017 0.002 0.0213 0.0002 0.0033 0.0019 0.0002 0.0055 0.0068 0.001 0.0048 0.0048 0.0032 0.0026
Lipid Particles 0.0052 0.0004 0.0019 0.0002 0.0007 0.0029 0.01 0 0.0047 0.0019 0 0.0011 0.0133 0 0.0024 0.0054 0.0014 0.0007
Mitochondria 0.0025 0.0006 0.0009 0.0008 0.0009 0.0042 0.0131 0.0001 0.0146 0.0149 0.0001 0.0119 0.0039 0.0002 0.0066 0.0016 0.0013 0.0064
None 0.0076 0.0017 0.0071 0.0026 0.008 0.003 0.0043 0.0009 0.0047 0.0014 0.0005 0.0008 0.0164 0.0042 0.0025 0.002 0.0325 0.0012
Nuclear Periphery 0.01 0.001 0.0014 0.0037 0.0011 0.0029 0.0117 0.0004 0.0008 0.0012 0.0006 0.0022 0.0057 0.0003 0.0015 0.0011 0.0017 0.0009
Nucleolus 0.0002 0.0002 0.0002 0.0001 0.0003 0.0003 0.0001 0 0.0001 0.0005 0 0.0001 0.0013 0 0.0008 0 0.0002 0
Nucleus 0.0026 0.0011 0.0018 0.0029 0.0017 0.0013 0.0027 0.0008 0.0009 0.0017 0.0008 0.0015 0.0021 0.0005 0.0014 0.0008 0.0014 0.0006
Peroxisomes 0.0035 0 0.0013 0.0001 0.0007 0.0004 0.0053 0 0.0025 0.0006 0 0.0005 0.0067 0 0.0019 0.0008 0.0006 0.0001
Punctate Nuclear 0.0015 0.0001 0.0007 0.0003 0.0007 0.0009 0.0008 0.0001 0.0002 0.0001 0 0.0004 0.0074 0.0001 0.0003 0.0003 0.0015 0.0001
Vacuole 0.0156 0.0133 0.0126 0.035 0.0152 0.0052 0.0116 0.0031 0.0105 0.0165 0.0017 0.0031 0.0055 0.0027 0.0047 0.007 0.0043 0.0021
Vacuole Periphery 0.0021 0.0007 0.0015 0.0054 0.0011 0.0014 0.0022 0.0002 0.0012 0.0023 0.0004 0.0008 0.001 0.0004 0.0013 0.0014 0.0008 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3689 4.27 5.545 5.096 5.419 12.7307 11.2433 10.4417 9.063 8.719
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
931 712 140 425 751 1240 939 106 1682 1952 1079 531

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 674.24 1298.03 1121.70 873.46 677.07 907.65 831.51 865.87 675.50 1050.04 869.16 871.94
Standard Deviation 116.55 350.85 226.12 112.65 138.49 182.47 95.85 133.41 126.82 318.37 155.36 117.13
Intensity Change Log 2 — 0.944990 0.734353 0.373479 — 0.422831 0.296429 0.354845 — 0.706865 0.531488 0.364173

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000098 0.001202 0.001423 0.000878 0.000261 0.000239 0.000126 0.000115 0.000171 0.000590 0.000294 0.000726
Bud Neck 0.004650 0.002477 0.003847 0.011285 0.008172 0.004245 0.001670 0.004120 0.006223 0.003600 0.001953 0.009855
Bud Site 0.007559 0.038763 0.018444 0.064634 0.017447 0.026138 0.003470 0.006007 0.011974 0.030743 0.005413 0.052931
Cell Periphery 0.000173 0.000914 0.000511 0.000134 0.000204 0.000139 0.000129 0.000127 0.000187 0.000421 0.000178 0.000132
Cytoplasm 0.665984* 0.794631* 0.515849* 0.506143* 0.598798* 0.716382* 0.481262* 0.411848* 0.635986* 0.744923* 0.485749* 0.487319*
Cytoplasmic Foci 0.180884* 0.103831 0.062580 0.045712 0.196360* 0.164519 0.021006 0.074327 0.187794* 0.142383 0.026400 0.051425
Eisosomes 0.000095 0.000016 0.000052 0.000052 0.000096 0.000032 0.000050 0.000091 0.000095 0.000026 0.000051 0.000059
Endoplasmic Reticulum 0.000839 0.001353 0.006021 0.001607 0.002134 0.000823 0.001747 0.001274 0.001417 0.001016 0.002302 0.001540
Endosome 0.009408 0.008668 0.008284 0.011490 0.027076 0.018123 0.002577 0.009950 0.017296 0.014674 0.003318 0.011182
Golgi 0.002308 0.008572 0.001374 0.001209 0.007360 0.010037 0.000064 0.000206 0.004564 0.009503 0.000234 0.001009
Lipid Particles 0.004266 0.002335 0.004086 0.001513 0.004409 0.001193 0.001470 0.002546 0.004330 0.001609 0.001809 0.001719
Mitochondria 0.001351 0.000113 0.000272 0.000937 0.003708 0.002254 0.000448 0.000113 0.002404 0.001473 0.000425 0.000772
Mitotic Spindle 0.000095 0.002373 0.006330 0.031752 0.001236 0.002359 0.001728 0.001694 0.000604 0.002364 0.002326 0.025752
None 0.010359 0.000610 0.005972 0.006593 0.010312 0.001524 0.005158 0.000615 0.010338 0.001191 0.005264 0.005399
Nuclear Periphery 0.000208 0.000024 0.000826 0.000912 0.000267 0.000052 0.001510 0.000925 0.000235 0.000042 0.001422 0.000915
Nuclear Periphery Foci 0.000896 0.000882 0.010456 0.001247 0.000794 0.000501 0.000816 0.002049 0.000850 0.000640 0.002067 0.001407
Nucleolus 0.000583 0.000144 0.000203 0.000424 0.001301 0.000837 0.000497 0.000597 0.000903 0.000584 0.000459 0.000459
Nucleus 0.060029 0.002595 0.210444* 0.109800 0.035083 0.005020 0.399180* 0.175093* 0.048891 0.004135 0.374691* 0.122834
Peroxisomes 0.001502 0.000957 0.000468 0.011347 0.002369 0.001992 0.000178 0.000939 0.001889 0.001615 0.000215 0.009270
Vacuole 0.047516 0.026326 0.141715 0.191735 0.077763 0.040444 0.076689 0.306602* 0.061021 0.035295 0.085126 0.214666*
Vacuole Periphery 0.001197 0.003214 0.000841 0.000595 0.004850 0.003149 0.000224 0.000763 0.002828 0.003172 0.000304 0.000629

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.13 -9.46 -3.85 1.30 5.68 0.32 0.85 1.05 1.50 0.55 -2.18 -4.96 -3.00 0.69 -0.70
Bud Neck 2.29 0.55 -0.93 -2.51 -1.31 4.51 6.86 4.43 1.15 -0.82 4.39 6.71 0.93 -1.75 -2.73
Bud Site -9.80 -4.14 -3.85 2.24 -1.55 -4.28 8.95 5.90 9.62 -1.34 -10.98 5.59 -2.61 2.10 -4.35
Cell Periphery -3.18 -6.18 5.22 3.45 7.40 3.90 5.18 6.44 3.35 2.66 -2.73 1.07 7.66 3.67 5.87
Cytoplasm -11.37 9.08 20.88 27.88 3.81 -9.86 16.38 9.91 14.78 1.72 -13.32 23.54 22.08 30.34 3.57
Cytoplasmic Foci 10.76 16.19 26.95 13.81 4.34 3.98 28.18 15.77 13.46 -5.67 8.37 39.26 33.31 24.83 -3.12
Eisosomes 12.19 2.46 4.94 -9.77 1.00 4.08 2.00 0.60 -7.42 -2.86 9.01 3.82 3.20 -12.61 -1.18
Endoplasmic Reticulum -1.80 -6.63 -6.48 -3.44 4.82 2.79 -2.01 0.71 -2.90 3.86 1.59 -7.49 -3.53 -5.68 4.05
Endosome 0.72 0.61 4.40 2.98 2.62 3.71 11.84 7.50 5.20 -2.33 1.99 13.52 9.57 8.00 -2.01
Golgi -5.96 2.05 3.24 9.22 2.48 -1.75 7.33 7.24 8.33 -1.76 -5.03 7.92 7.53 11.10 -1.20
Lipid Particles 3.00 -0.35 5.80 1.03 4.36 6.62 5.61 3.94 -3.24 -1.64 7.44 6.86 7.35 -1.05 1.16
Mitochondria 2.55 2.15 1.48 -4.26 -2.44 1.27 4.10 4.51 2.64 5.11 1.35 4.43 4.15 1.84 -0.92
Mitotic Spindle -2.99 -2.47 -5.30 -4.71 -4.11 -1.24 -1.63 -0.77 0.45 0.68 -3.05 -3.49 -5.22 -4.72 -4.64
None 5.92 2.11 2.09 -4.52 -0.82 3.48 2.42 3.68 0.93 5.02 6.29 4.05 3.02 -3.99 -0.95
Nuclear Periphery 4.76 -8.01 -14.41 -16.31 -5.47 3.76 -21.40 -5.92 -7.54 6.11 5.63 -24.66 -15.24 -17.43 1.36
Nuclear Periphery Foci 0.05 -8.36 -4.44 -3.88 7.67 2.60 -2.80 -2.24 -2.88 -1.49 2.39 -7.57 -5.13 -6.29 2.90
Nucleolus 3.61 6.74 1.88 -1.95 -2.43 0.78 3.81 3.67 0.92 0.56 0.87 4.08 4.22 0.58 0.25
Nucleus 19.03 -7.27 -10.79 -17.47 2.12 9.43 -39.94 -5.63 -7.54 15.54 20.19 -38.66 -12.95 -18.87 20.05
Peroxisomes 1.28 2.47 -1.13 -1.60 -1.96 0.22 3.90 2.70 2.54 -2.04 0.29 4.94 -0.63 -0.75 -2.26
Vacuole 5.97 -8.88 -21.44 -22.83 -6.81 7.76 -8.36 -13.22 -14.71 -10.75 8.85 -13.81 -24.31 -26.42 -16.41
Vacuole Periphery -3.85 2.26 5.71 5.91 2.14 2.82 9.30 7.83 6.44 -1.05 -0.93 10.48 10.04 10.05 -0.52
Standard name
Human Ortholog
Description Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yat2

Yat2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yat2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available