ORF
Human Ortholog
Description Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.86 0.99 0.95 0.94 0.89 0.9 0.88 0.72 0.73 0.59 0.59 0.94 0.99 0.99 – 0.99 0.93 0.84 0.84 0.69 0.91 0.89 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.09 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.18 0 0.07 0.06 0.16 0.11 0.15 0.49 0.45 0.66 0.63 0 0 0 – 0 0 0.05 0.07 0.15 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 – 0 0 0 0 0 0 0 1
Bud 2 1 1 1 0 0 0 1 9 5 3 1 0 0 0 – 0 1 0 0 0 0 2 4
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 1 1 3 3 4 1 1 1 3 0 0 1 2 – 2 5 0 0 0 0 0 0
Cytoplasm 103 65 88 125 117 128 193 276 155 179 82 101 17 200 262 – 75 111 102 90 82 99 160 210
Endoplasmic Reticulum 4 3 1 0 0 0 1 0 2 0 0 2 0 2 1 – 2 11 4 1 7 0 1 3
Endosome 0 0 0 0 0 2 5 2 0 1 1 0 0 1 1 – 0 0 1 1 1 0 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Mitochondria 9 14 0 9 7 23 24 47 105 109 92 109 0 0 0 – 3 3 5 8 17 3 3 6
Nucleus 0 0 1 3 4 2 2 4 2 2 3 4 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 1 1 0 0 1 0 1 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 2 0 0 0 1 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 – 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 0 0 2 2 5 6 10 3 2 1 2 0 0 1 – 0 3 3 2 3 1 2 4
Unique Cell Count 110 76 89 132 125 144 214 312 216 244 140 172 18 203 265 76 119 122 107 119 109 180 245
Labelled Cell Count 119 85 92 142 135 163 236 344 280 301 188 220 18 204 268 82 135 122 107 119 109 180 245


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 3.8 5.6 5.3 6.3 5.2 5.2 5.4 4.2 4.5 4.2 4.2 4.8 6.2 5.6 4.2 5.2 5.8 6.3 6.7 6.6
Std Deviation (1e-4) 0.7 0.6 1.2 1.3 1.8 1.6 1.4 1.9 1.1 1.3 1.3 1.0 0.5 2.2 1.5 0.8 0.8 1.3 1.5 1.9 1.9
Intensity Change (Log2) – – – -0.06 0.18 -0.09 -0.1 -0.03 -0.42 -0.31 -0.4 -0.42 -0.21 0.17 0.01 -0.42 -0.09 0.05 0.18 0.27 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4919 0.2012 -0.0478 -0.0705 -0.0708 0.089 2.5487 3.3523 3.2322 2.8434 3.512 3.5524 0.0952 0.8477 0.5316 1.092 0.3859 0.6793
Actin 0.004 0.0006 0.0008 0.0075 0.0259 0.007 0.0339 0.0028 0.0081 0.0003 0.0236 0.0052 0.0078 0.0014 0.011 0.0114 0.0292 0.0014
Bud 0.0005 0.0006 0.0027 0.0003 0.0003 0.0006 0.0006 0.0008 0.0026 0.0008 0.0017 0.0006 0.0005 0.0002 0.0014 0.0013 0.0003 0
Bud Neck 0.0017 0.0003 0.0002 0.0002 0.0009 0.0039 0.0101 0.0001 0.0003 0.0004 0.0016 0.0007 0.0004 0.0009 0.0001 0.0005 0.0004 0.0003
Bud Periphery 0.0005 0.0005 0.0053 0.0003 0.0005 0.0006 0.001 0.0013 0.0057 0.0006 0.0032 0.001 0.0007 0.0002 0.0033 0.0017 0.0004 0
Bud Site 0.0066 0.0033 0.0015 0.0004 0.0008 0.0006 0.0085 0.0012 0.0026 0.0006 0.0102 0.0002 0.001 0.0128 0.0004 0.0008 0.0003 0
Cell Periphery 0.0003 0.0003 0.0003 0.0002 0.0002 0.0002 0.0003 0.0002 0.0003 0.0003 0.0006 0.0001 0.0004 0.0003 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.4974 0.5701 0.5475 0.5576 0.4207 0.6537 0.2884 0.5126 0.364 0.4274 0.3001 0.4376 0.3633 0.5524 0.596 0.5115 0.569 0.6783
Cytoplasmic Foci 0.0259 0.0263 0.0172 0.0285 0.0516 0.0335 0.0389 0.0119 0.0159 0.0139 0.0164 0.0104 0.0277 0.0155 0.0071 0.0275 0.0075 0.0105
Eisosomes 0.0004 0.0001 0.0001 0.0033 0.0016 0.0001 0.0004 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0069 0.0031 0.0053 0.003 0.0026 0.0037 0.0023 0.0016 0.0057 0.0022 0.0029 0.0023 0.0056 0.0026 0.003 0.0052 0.0046 0.0025
Endosome 0.0151 0.0171 0.0165 0.0619 0.0233 0.015 0.0129 0.0031 0.0079 0.0152 0.0101 0.0089 0.0246 0.0022 0.0053 0.0403 0.0112 0.0063
Golgi 0.0015 0.001 0.0016 0.0019 0.0034 0.001 0.0046 0.0009 0.0015 0.0004 0.0035 0.0014 0.0077 0.0005 0.0033 0.0069 0.0056 0.0012
Lipid Particles 0.003 0.0008 0.0013 0.0038 0.0093 0.0013 0.0077 0.0018 0.0014 0.0002 0.0043 0.0011 0.0249 0.0007 0.0006 0.003 0.0016 0.0054
Mitochondria 0.001 0.0005 0.0013 0.0015 0.0134 0.0007 0.0014 0.0007 0.0028 0.0005 0.0014 0.0013 0.0027 0.0003 0.0069 0.0036 0.0017 0.0004
None 0.4244 0.3665 0.3895 0.3201 0.4021 0.2727 0.5512 0.4566 0.57 0.5274 0.5699 0.5232 0.5149 0.4033 0.3572 0.3533 0.3645 0.2902
Nuclear Periphery 0.0019 0.0008 0.0014 0.0008 0.0011 0.0008 0.0057 0.0003 0.0031 0.0006 0.0055 0.0023 0.0034 0.0003 0.0006 0.0028 0.0008 0.0004
Nucleolus 0.0005 0.0003 0.0002 0.0001 0.0006 0.0001 0.0008 0.0001 0.0002 0.0002 0.0008 0.0001 0.0003 0.0001 0 0.0001 0 0
Nucleus 0.0012 0.0015 0.0013 0.0008 0.0018 0.0009 0.0041 0.0006 0.0013 0.001 0.0207 0.0006 0.0008 0.0004 0.0006 0.001 0.0004 0.0004
Peroxisomes 0.0023 0.0002 0.0002 0.0021 0.0163 0.0004 0.0155 0.001 0.0019 0.0002 0.0031 0.0006 0.0069 0.0044 0.0004 0.0206 0.0004 0.0011
Punctate Nuclear 0.0018 0.0008 0.0009 0.0005 0.0209 0.001 0.0078 0.0005 0.0019 0.0005 0.0188 0.0011 0.0022 0.0006 0.0002 0.0008 0.0003 0.0006
Vacuole 0.0028 0.0049 0.0041 0.0044 0.002 0.0019 0.0035 0.0015 0.0019 0.0062 0.0011 0.001 0.0031 0.001 0.0021 0.006 0.0012 0.0006
Vacuole Periphery 0.0004 0.0006 0.0007 0.001 0.0006 0.0004 0.0006 0.0002 0.0005 0.0008 0.0003 0.0004 0.0009 0.0001 0.0002 0.0014 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
107 1034 230 2244 1461 2394 845 1384 1568 3428 1075 3628

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.34 742.62 938.36 844.74 642.04 839.28 872.69 844.22 654.34 810.12 886.74 844.54
Standard Deviation 184.53 109.21 98.70 100.11 114.49 89.84 108.13 108.16 128.86 105.84 109.55 103.25
Intensity Change Log 2 — -0.147111 0.190407 0.038772 — 0.386489 0.442806 0.394956 — 0.111368 0.306536 0.205845

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000780 0.000421 0.000342 0.001002 0.000111 0.000358 0.000178 0.000252 0.000157 0.000377 0.000213 0.000716
Bud Neck 0.030023 0.022027 0.001774 0.005446 0.007373 0.025383 0.001111 0.002172 0.008918 0.024371 0.001253 0.004197
Bud Site 0.065075 0.029133 0.004651 0.029128 0.007219 0.017634 0.004799 0.017715 0.011167 0.021103 0.004768 0.024774
Cell Periphery 0.000838 0.000159 0.000207 0.000100 0.000154 0.000130 0.000122 0.000116 0.000201 0.000139 0.000140 0.000106
Cytoplasm 0.323311* 0.329289* 0.569904* 0.663100* 0.549780* 0.465281* 0.676859* 0.681471* 0.534326* 0.424261* 0.653975* 0.670108*
Cytoplasmic Foci 0.250201* 0.355470* 0.063964 0.034042 0.231006* 0.282927* 0.065138 0.084246 0.232316* 0.304808* 0.064887 0.053194
Eisosomes 0.000498 0.000139 0.000068 0.000035 0.000090 0.000185 0.000045 0.000038 0.000118 0.000171 0.000050 0.000036
Endoplasmic Reticulum 0.016166 0.001953 0.004014 0.000774 0.002837 0.001167 0.001706 0.000880 0.003746 0.001404 0.002200 0.000814
Endosome 0.076031 0.067954 0.010197 0.003478 0.014802 0.030256 0.006064 0.006899 0.018980 0.041627 0.006948 0.004783
Golgi 0.034658 0.020667 0.000181 0.000720 0.005422 0.010676 0.000125 0.001014 0.007417 0.013689 0.000137 0.000832
Lipid Particles 0.022678 0.009717 0.003515 0.000773 0.009314 0.006727 0.001787 0.001280 0.010226 0.007629 0.002156 0.000966
Mitochondria 0.010991 0.013445 0.000170 0.002128 0.004271 0.007113 0.000100 0.000165 0.004729 0.009023 0.000115 0.001379
Mitotic Spindle 0.024704 0.003966 0.000682 0.025129 0.000488 0.002716 0.000478 0.003706 0.002140 0.003093 0.000522 0.016957
None 0.002026 0.001227 0.002836 0.003882 0.016469 0.001663 0.003161 0.002574 0.015484 0.001531 0.003091 0.003383
Nuclear Periphery 0.002867 0.000316 0.001380 0.000540 0.000492 0.000136 0.000634 0.000301 0.000654 0.000191 0.000793 0.000449
Nuclear Periphery Foci 0.014007 0.001010 0.007407 0.000594 0.001324 0.000151 0.004273 0.002324 0.002190 0.000410 0.004944 0.001254
Nucleolus 0.006575 0.002355 0.000401 0.000254 0.002583 0.000552 0.000223 0.000210 0.002856 0.001096 0.000261 0.000237
Nucleus 0.027665 0.023264 0.171842* 0.088548 0.085415 0.042449 0.122672 0.058519 0.081474 0.036662 0.133192* 0.077093
Peroxisomes 0.011663 0.011202 0.000506 0.001666 0.001822 0.005588 0.000298 0.000503 0.002493 0.007282 0.000342 0.001222
Vacuole 0.065502 0.096458 0.155665 0.138235 0.056328 0.095636 0.110076 0.135114 0.056954 0.095884 0.119830 0.137045
Vacuole Periphery 0.013743 0.009827 0.000292 0.000426 0.002700 0.003272 0.000151 0.000501 0.003454 0.005249 0.000181 0.000455

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.04 2.20 1.01 -1.22 -1.44 -2.19 -1.48 -2.91 0.96 0.52 -2.52 -1.45 -2.87 -0.84 -1.03
Bud Neck 0.98 3.53 3.23 10.57 -4.97 -13.13 10.21 8.35 18.60 -5.33 -12.28 9.49 6.32 20.23 -8.33
Bud Site 2.72 4.61 3.54 4.00 -7.63 -7.57 1.76 -3.93 0.98 -4.59 -6.46 4.27 -3.66 2.29 -7.95
Cell Periphery 5.03 4.62 5.65 7.79 5.26 2.37 2.94 4.32 2.72 1.86 4.49 4.09 8.20 7.88 6.35
Cytoplasm -0.36 -5.54 -9.59 -26.60 -4.70 9.69 -8.36 -10.56 -21.24 -0.98 13.39 -7.44 -10.07 -29.53 -0.49
Cytoplasmic Foci -4.96 8.86 10.41 47.49 5.45 -7.82 29.52 26.33 38.47 -4.31 -11.89 31.49 36.47 64.22 4.39
Eisosomes 3.76 4.42 4.88 12.80 6.39 -9.26 7.57 10.25 16.22 3.54 -4.94 7.34 9.84 19.90 6.83
Endoplasmic Reticulum 2.69 2.10 2.90 5.46 8.01 3.91 1.96 4.68 1.70 6.98 4.34 1.99 5.45 4.61 10.80
Endosome 0.63 5.53 6.21 20.65 7.03 -10.22 8.61 8.98 18.57 -0.23 -12.68 9.32 12.38 28.33 5.59
Golgi 2.16 5.53 5.49 12.54 -1.65 -7.81 14.39 7.68 13.43 -1.47 -7.37 12.69 11.28 19.93 -2.14
Lipid Particles 2.79 4.23 4.87 9.19 6.86 3.25 10.56 11.68 12.67 4.13 3.11 10.91 12.88 15.81 8.59
Mitochondria -0.83 4.15 3.67 7.06 -3.02 -2.93 5.92 5.85 10.37 -2.17 -4.44 6.77 5.35 11.18 -3.23
Mitotic Spindle 2.09 2.43 0.82 -5.30 -8.06 -3.59 -0.58 -2.86 -0.77 -2.74 -0.98 2.10 -6.47 -6.09 -8.51
None 0.95 -0.38 -1.39 -3.44 -1.21 7.24 6.45 6.82 -0.81 0.69 7.36 6.55 6.61 -2.71 -0.19
Nuclear Periphery 1.67 0.71 1.24 -5.83 4.69 6.62 -4.02 2.12 -9.45 8.48 3.95 -2.59 -0.69 -9.43 3.30
Nuclear Periphery Foci 2.27 1.16 2.34 1.67 6.39 7.87 -9.13 -4.19 -12.67 6.38 4.06 -5.36 2.11 -8.93 11.13
Nucleolus 1.75 2.68 2.71 3.53 1.11 6.79 7.93 7.95 5.83 0.41 4.45 8.03 7.97 4.39 -0.77
Nucleus 0.95 -9.65 -8.29 -26.84 5.49 11.64 -7.73 5.08 -8.76 12.94 12.96 -10.62 -3.37 -25.90 9.41
Peroxisomes 0.37 3.80 3.63 10.25 -1.23 -8.53 10.40 9.41 12.18 -1.71 -9.82 8.42 5.19 13.93 -1.68
Vacuole -3.09 -8.60 -14.18 -17.73 0.08 -11.57 -12.69 -19.68 -12.71 -3.34 -12.54 -15.76 -31.54 -23.19 -5.47
Vacuole Periphery 1.18 4.19 4.20 12.45 0.50 -2.14 12.00 9.57 14.69 -2.97 -4.48 10.83 10.20 18.43 -3.34
ORF
Human Ortholog
Description Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJR011C

YJR011C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr011c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available