ORF | |
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Human Ortholog | |
Description | Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | – | 1 | 0 | 2 | 3 | – | 2 | 0 | 3 | 2 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | – | 0 | 2 | 1 | 7 | – | 3 | 4 | 3 | 1 | – | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | – | 0 | 2 | 0 | 0 | – | 2 | 0 | 0 | 3 | – | – | 3 | 35 | 24 | 17 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 136 | 160 | – | 149 | 242 | 214 | 327 | – | 179 | 268 | 135 | 182 | – | – | 172 | 334 | 342 | 343 | 130 | 156 | 31 | 23 | 73 | 80 |
Endoplasmic Reticulum | 1 | 1 | – | 0 | 2 | 0 | 2 | – | 1 | 1 | 2 | 0 | – | – | 1 | 23 | 11 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 |
Golgi | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 0 | – | 1 | 0 | 11 | 11 | – | 43 | 35 | 96 | 75 | – | – | 0 | 3 | 3 | 5 | 2 | 1 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | – | 1 | 0 | 4 | 3 | – | 3 | 1 | 1 | 4 | – | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 3 | 4 | 0 | 7 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | – | 0 | 1 | 2 | 1 | – | 0 | 1 | 1 | 3 | – | – | 0 | 11 | 7 | 2 | 2 | 1 | 1 | 0 | 0 | 2 |
Unique Cell Count | 144 | 163 | 150 | 244 | 225 | 339 | 211 | 295 | 195 | 228 | 173 | 349 | 349 | 352 | 143 | 164 | 37 | 26 | 81 | 85 | ||||
Labelled Cell Count | 153 | 165 | 152 | 249 | 235 | 355 | 238 | 315 | 241 | 278 | 178 | 411 | 391 | 391 | 143 | 164 | 37 | 26 | 81 | 85 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.8 | – | 5.5 | 6.0 | 4.7 | 4.8 | – | 4.0 | 4.1 | 3.8 | 3.8 | – | – | 5.7 | 7.8 | 7.5 | 6.7 | – | 4.7 | 5.7 |
Std Deviation (1e-4) | 0.7 | 0.9 | – | 1.3 | 1.7 | 1.3 | 1.3 | – | 1.1 | 0.7 | 1.1 | 0.9 | – | – | 1.3 | 1.2 | 1.3 | 1.2 | – | 0.7 | 1.6 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8047 | 0.6288 | 0.7222 | 0.5526 | 0.5215 | 0.5282 | 0.4883 | 0.5884 | 0.538 | 0.8956 | 0.1701 | 0.4475 | -0.6987 | -0.7096 | -0.8782 | -0.9942 | -0.8584 | -0.5665 |
Actin | 0.0262 | 0.0025 | 0.0106 | 0.0064 | 0.0083 | 0.0046 | 0.0608 | 0.0001 | 0.0022 | 0.0107 | 0.0001 | 0.0027 | 0.0369 | 0.0013 | 0.0271 | 0.0535 | 0.0005 | 0.0273 |
Bud | 0.0061 | 0.0134 | 0.0162 | 0.0044 | 0.0009 | 0.0033 | 0.0009 | 0.0001 | 0.0005 | 0.0065 | 0 | 0.0008 | 0.0013 | 0.0078 | 0.0002 | 0.0001 | 0.0009 | 0.0003 |
Bud Neck | 0.0188 | 0.0006 | 0.0018 | 0.0013 | 0.0006 | 0.0012 | 0.0049 | 0.0001 | 0.0003 | 0.0011 | 0.0001 | 0.0054 | 0.0032 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0011 |
Bud Periphery | 0.0107 | 0.0081 | 0.0064 | 0.0174 | 0.0017 | 0.0046 | 0.0011 | 0.0001 | 0.0012 | 0.0268 | 0 | 0.0012 | 0.0018 | 0.0081 | 0.0002 | 0.0002 | 0.0014 | 0.0003 |
Bud Site | 0.008 | 0.0246 | 0.0215 | 0.0005 | 0.0025 | 0.0017 | 0.012 | 0.0008 | 0.0125 | 0.0042 | 0 | 0.0037 | 0.0097 | 0.0046 | 0.0009 | 0.0002 | 0.0013 | 0.0005 |
Cell Periphery | 0.0006 | 0.0005 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.1494 | 0.304 | 0.1949 | 0.1778 | 0.1015 | 0.3124 | 0.1989 | 0.4091 | 0.2937 | 0.1915 | 0.4819 | 0.3946 | 0.1741 | 0.4156 | 0.3962 | 0.298 | 0.3218 | 0.4475 |
Cytoplasmic Foci | 0.0374 | 0.0161 | 0.0191 | 0.0086 | 0.0209 | 0.0169 | 0.0475 | 0.0066 | 0.0237 | 0.0455 | 0.0052 | 0.0275 | 0.0669 | 0.0045 | 0.012 | 0.0717 | 0.0074 | 0.036 |
Eisosomes | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0043 | 0.0015 | 0.0012 | 0.003 | 0.0024 | 0.0014 | 0.0026 | 0.0027 | 0.0021 | 0.0037 | 0.0011 | 0.0008 | 0.0102 | 0.0009 | 0.0024 | 0.0018 | 0.0058 | 0.0029 |
Endosome | 0.0279 | 0.0041 | 0.0083 | 0.0103 | 0.0768 | 0.008 | 0.0255 | 0.0046 | 0.0227 | 0.0785 | 0.0015 | 0.0043 | 0.0229 | 0.0015 | 0.0091 | 0.019 | 0.048 | 0.006 |
Golgi | 0.0082 | 0.0005 | 0.0034 | 0.0015 | 0.0258 | 0.0026 | 0.0124 | 0.0003 | 0.006 | 0.0559 | 0.0001 | 0.0049 | 0.0061 | 0.0002 | 0.0034 | 0.005 | 0.0021 | 0.0092 |
Lipid Particles | 0.0095 | 0.0005 | 0.0074 | 0.0002 | 0.0278 | 0.0018 | 0.0243 | 0.0003 | 0.0022 | 0.0242 | 0 | 0.0111 | 0.0181 | 0.0001 | 0.0073 | 0.0077 | 0.0011 | 0.006 |
Mitochondria | 0.0125 | 0.0008 | 0.0168 | 0.0025 | 0.016 | 0.0028 | 0.0319 | 0.0002 | 0.0144 | 0.0186 | 0.0001 | 0.0199 | 0.0058 | 0.0009 | 0.0041 | 0.0017 | 0.0065 | 0.0039 |
None | 0.6191 | 0.6103 | 0.6674 | 0.7568 | 0.6645 | 0.6207 | 0.5021 | 0.5705 | 0.6018 | 0.4887 | 0.5084 | 0.5125 | 0.5767 | 0.5503 | 0.528 | 0.5161 | 0.5526 | 0.4519 |
Nuclear Periphery | 0.0123 | 0.0008 | 0.0017 | 0.0014 | 0.0031 | 0.0021 | 0.0023 | 0.0004 | 0.0006 | 0.0034 | 0.0002 | 0.0002 | 0.0149 | 0.0004 | 0.001 | 0.0012 | 0.0106 | 0.0006 |
Nucleolus | 0.0049 | 0.0014 | 0.0009 | 0.0001 | 0.001 | 0.0015 | 0.0029 | 0.0001 | 0.0002 | 0.0009 | 0 | 0.0008 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0003 |
Nucleus | 0.0124 | 0.0028 | 0.0026 | 0.0012 | 0.0049 | 0.0036 | 0.0108 | 0.0009 | 0.0007 | 0.0019 | 0.0004 | 0.0005 | 0.0069 | 0.0008 | 0.0006 | 0.0007 | 0.0058 | 0.0005 |
Peroxisomes | 0.0196 | 0.0009 | 0.0101 | 0.0002 | 0.0224 | 0.0018 | 0.0407 | 0 | 0.0098 | 0.0233 | 0 | 0.0062 | 0.018 | 0.0002 | 0.0032 | 0.016 | 0.0002 | 0.0036 |
Punctate Nuclear | 0.0055 | 0.0042 | 0.0053 | 0.0004 | 0.0077 | 0.0068 | 0.01 | 0.0004 | 0.0017 | 0.0037 | 0.0002 | 0.001 | 0.0177 | 0.0002 | 0.0009 | 0.0037 | 0.0005 | 0.001 |
Vacuole | 0.0041 | 0.0021 | 0.002 | 0.0041 | 0.007 | 0.0015 | 0.0065 | 0.0022 | 0.0023 | 0.0078 | 0.0005 | 0.0013 | 0.0038 | 0.002 | 0.002 | 0.0023 | 0.0222 | 0.0007 |
Vacuole Periphery | 0.002 | 0.0003 | 0.001 | 0.0015 | 0.0037 | 0.0005 | 0.0013 | 0.0002 | 0.001 | 0.0021 | 0.0001 | 0.0003 | 0.0022 | 0.0002 | 0.0004 | 0.0005 | 0.0103 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.1382 | 2.8054 | 6.2452 | 8.7966 | 5.6964 | 2.5092 | 7.9423 | 9.1894 | 10.2932 | 8.3756 |
Translational Efficiency | 1.678 | 2.2744 | 0.9773 | 0.5396 | 0.7651 | 1.7354 | 0.6789 | 0.6278 | 0.5919 | 0.7473 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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