ORF
Human Ortholog
Description Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0.1 0.07 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.98 – 0.99 0.99 0.95 0.96 – 0.85 0.91 0.69 0.8 – – 0.99 0.96 0.98 0.97 0.91 0.95 0.86 0.89 0.91 0.94
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0.07 0 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 – 0 0 0.05 0 – 0.2 0.12 0.49 0.33 – – 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 1 1 – 0 0 0 0 – – 0 1 0 0 0 0 0 0 0 0
Bud 1 0 – 1 0 2 3 – 2 0 3 2 – – 0 0 1 0 0 0 0 1 1 0
Bud Neck 0 0 – 0 2 1 7 – 3 4 3 1 – – 1 0 1 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 1 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 3 – 0 2 0 0 – 2 0 0 3 – – 3 35 24 17 0 0 0 0 0 0
Cytoplasm 136 160 – 149 242 214 327 – 179 268 135 182 – – 172 334 342 343 130 156 31 23 73 80
Endoplasmic Reticulum 1 1 – 0 2 0 2 – 1 1 2 0 – – 1 23 11 20 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 1 – – 0 2 1 2 1 0 0 0 2 0
Golgi 1 0 – 0 0 0 0 – 0 0 0 0 – – 0 1 0 1 0 0 0 0 0 0
Mitochondria 13 0 – 1 0 11 11 – 43 35 96 75 – – 0 3 3 5 2 1 1 0 0 0
Nucleus 0 0 – 1 0 4 3 – 3 1 1 4 – – 1 1 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 1 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 1 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 3 4 0 7 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 – 0 1 2 1 – 0 1 1 3 – – 0 11 7 2 2 1 1 0 0 2
Unique Cell Count 144 163 150 244 225 339 211 295 195 228 173 349 349 352 143 164 37 26 81 85
Labelled Cell Count 153 165 152 249 235 355 238 315 241 278 178 411 391 391 143 164 37 26 81 85


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.8 – 5.5 6.0 4.7 4.8 – 4.0 4.1 3.8 3.8 – – 5.7 7.8 7.5 6.7 – 4.7 5.7
Std Deviation (1e-4) 0.7 0.9 – 1.3 1.7 1.3 1.3 – 1.1 0.7 1.1 0.9 – – 1.3 1.2 1.3 1.2 – 0.7 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8047 0.6288 0.7222 0.5526 0.5215 0.5282 0.4883 0.5884 0.538 0.8956 0.1701 0.4475 -0.6987 -0.7096 -0.8782 -0.9942 -0.8584 -0.5665
Actin 0.0262 0.0025 0.0106 0.0064 0.0083 0.0046 0.0608 0.0001 0.0022 0.0107 0.0001 0.0027 0.0369 0.0013 0.0271 0.0535 0.0005 0.0273
Bud 0.0061 0.0134 0.0162 0.0044 0.0009 0.0033 0.0009 0.0001 0.0005 0.0065 0 0.0008 0.0013 0.0078 0.0002 0.0001 0.0009 0.0003
Bud Neck 0.0188 0.0006 0.0018 0.0013 0.0006 0.0012 0.0049 0.0001 0.0003 0.0011 0.0001 0.0054 0.0032 0.0001 0.0004 0.0001 0.0001 0.0011
Bud Periphery 0.0107 0.0081 0.0064 0.0174 0.0017 0.0046 0.0011 0.0001 0.0012 0.0268 0 0.0012 0.0018 0.0081 0.0002 0.0002 0.0014 0.0003
Bud Site 0.008 0.0246 0.0215 0.0005 0.0025 0.0017 0.012 0.0008 0.0125 0.0042 0 0.0037 0.0097 0.0046 0.0009 0.0002 0.0013 0.0005
Cell Periphery 0.0006 0.0005 0.0006 0.0003 0.0002 0.0002 0.0004 0.0002 0.0003 0.0007 0 0.0002 0.0004 0.0003 0.0001 0 0.0002 0.0001
Cytoplasm 0.1494 0.304 0.1949 0.1778 0.1015 0.3124 0.1989 0.4091 0.2937 0.1915 0.4819 0.3946 0.1741 0.4156 0.3962 0.298 0.3218 0.4475
Cytoplasmic Foci 0.0374 0.0161 0.0191 0.0086 0.0209 0.0169 0.0475 0.0066 0.0237 0.0455 0.0052 0.0275 0.0669 0.0045 0.012 0.0717 0.0074 0.036
Eisosomes 0.0004 0.0001 0.0007 0.0001 0.0002 0.0001 0.0006 0.0002 0.0002 0.0003 0 0.0001 0.0008 0.0001 0.0002 0.0003 0 0.0001
Endoplasmic Reticulum 0.0043 0.0015 0.0012 0.003 0.0024 0.0014 0.0026 0.0027 0.0021 0.0037 0.0011 0.0008 0.0102 0.0009 0.0024 0.0018 0.0058 0.0029
Endosome 0.0279 0.0041 0.0083 0.0103 0.0768 0.008 0.0255 0.0046 0.0227 0.0785 0.0015 0.0043 0.0229 0.0015 0.0091 0.019 0.048 0.006
Golgi 0.0082 0.0005 0.0034 0.0015 0.0258 0.0026 0.0124 0.0003 0.006 0.0559 0.0001 0.0049 0.0061 0.0002 0.0034 0.005 0.0021 0.0092
Lipid Particles 0.0095 0.0005 0.0074 0.0002 0.0278 0.0018 0.0243 0.0003 0.0022 0.0242 0 0.0111 0.0181 0.0001 0.0073 0.0077 0.0011 0.006
Mitochondria 0.0125 0.0008 0.0168 0.0025 0.016 0.0028 0.0319 0.0002 0.0144 0.0186 0.0001 0.0199 0.0058 0.0009 0.0041 0.0017 0.0065 0.0039
None 0.6191 0.6103 0.6674 0.7568 0.6645 0.6207 0.5021 0.5705 0.6018 0.4887 0.5084 0.5125 0.5767 0.5503 0.528 0.5161 0.5526 0.4519
Nuclear Periphery 0.0123 0.0008 0.0017 0.0014 0.0031 0.0021 0.0023 0.0004 0.0006 0.0034 0.0002 0.0002 0.0149 0.0004 0.001 0.0012 0.0106 0.0006
Nucleolus 0.0049 0.0014 0.0009 0.0001 0.001 0.0015 0.0029 0.0001 0.0002 0.0009 0 0.0008 0.0017 0.0001 0.0001 0.0001 0.0005 0.0003
Nucleus 0.0124 0.0028 0.0026 0.0012 0.0049 0.0036 0.0108 0.0009 0.0007 0.0019 0.0004 0.0005 0.0069 0.0008 0.0006 0.0007 0.0058 0.0005
Peroxisomes 0.0196 0.0009 0.0101 0.0002 0.0224 0.0018 0.0407 0 0.0098 0.0233 0 0.0062 0.018 0.0002 0.0032 0.016 0.0002 0.0036
Punctate Nuclear 0.0055 0.0042 0.0053 0.0004 0.0077 0.0068 0.01 0.0004 0.0017 0.0037 0.0002 0.001 0.0177 0.0002 0.0009 0.0037 0.0005 0.001
Vacuole 0.0041 0.0021 0.002 0.0041 0.007 0.0015 0.0065 0.0022 0.0023 0.0078 0.0005 0.0013 0.0038 0.002 0.002 0.0023 0.0222 0.0007
Vacuole Periphery 0.002 0.0003 0.001 0.0015 0.0037 0.0005 0.0013 0.0002 0.001 0.0021 0.0001 0.0003 0.0022 0.0002 0.0004 0.0005 0.0103 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.1382 2.8054 6.2452 8.7966 5.6964 2.5092 7.9423 9.1894 10.2932 8.3756
Translational Efficiency 1.678 2.2744 0.9773 0.5396 0.7651 1.7354 0.6789 0.6278 0.5919 0.7473

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1933 1056 1670 1098 1724 1779 1418 189 3657 2835 3088 1287

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.92 771.88 900.43 808.71 737.18 731.44 890.95 869.03 692.11 746.50 896.08 817.57
Standard Deviation 71.29 104.83 107.61 110.81 85.34 90.93 105.42 105.45 89.06 98.31 106.72 112.09
Intensity Change Log 2 — 0.243682 0.465919 0.310928 — -0.011277 0.273328 0.237389 — 0.114002 0.366921 0.272369

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000153 0.000621 0.000283 0.000927 0.000121 0.000420 0.000121 0.000254 0.000138 0.000495 0.000209 0.000828
Bud Neck 0.028228 0.024149 0.002662 0.008551 0.010521 0.028507 0.001200 0.009372 0.019880 0.026884 0.001991 0.008671
Bud Site 0.014011 0.031403 0.010833 0.042773 0.005892 0.028900 0.004476 0.016969 0.010184 0.029832 0.007914 0.038983
Cell Periphery 0.000200 0.000147 0.000085 0.000161 0.000134 0.000123 0.000070 0.000138 0.000169 0.000132 0.000078 0.000157
Cytoplasm 0.527934* 0.417163* 0.538965* 0.622455* 0.569760* 0.450459* 0.684260* 0.518845* 0.547652* 0.438057* 0.605684* 0.607239*
Cytoplasmic Foci 0.232150* 0.278115* 0.006785 0.029578 0.221594* 0.252445* 0.015033 0.042307 0.227173* 0.262007* 0.010572 0.031447
Eisosomes 0.000259 0.000116 0.000018 0.000041 0.000138 0.000142 0.000021 0.000069 0.000202 0.000132 0.000020 0.000045
Endoplasmic Reticulum 0.001426 0.000671 0.002226 0.000986 0.000976 0.000448 0.001261 0.000683 0.001214 0.000531 0.001783 0.000942
Endosome 0.019368 0.051733 0.001354 0.006825 0.017195 0.046508 0.001984 0.006209 0.018343 0.048454 0.001643 0.006735
Golgi 0.005379 0.008668 0.000081 0.006755 0.003569 0.009982 0.000163 0.000548 0.004526 0.009493 0.000118 0.005844
Lipid Particles 0.007237 0.003926 0.000679 0.000802 0.004601 0.003761 0.000804 0.001160 0.005994 0.003823 0.000736 0.000855
Mitochondria 0.005293 0.004274 0.000474 0.002626 0.001927 0.006962 0.000294 0.003540 0.003706 0.005961 0.000391 0.002760
Mitotic Spindle 0.001172 0.009604 0.004214 0.017234 0.001561 0.010267 0.008909 0.016631 0.001356 0.010020 0.006370 0.017145
None 0.007254 0.001376 0.005062 0.006923 0.002209 0.000709 0.005700 0.010609 0.004875 0.000958 0.005355 0.007464
Nuclear Periphery 0.000218 0.000163 0.001166 0.000455 0.000188 0.000201 0.000752 0.000536 0.000204 0.000187 0.000976 0.000467
Nuclear Periphery Foci 0.000443 0.000566 0.001577 0.001410 0.000480 0.000263 0.001873 0.000872 0.000460 0.000376 0.001713 0.001331
Nucleolus 0.001259 0.000846 0.000209 0.000244 0.000544 0.000781 0.000118 0.000278 0.000922 0.000806 0.000167 0.000249
Nucleus 0.064873 0.029079 0.381443* 0.116507 0.069922 0.025262 0.196517* 0.094644 0.067253 0.026683 0.296526* 0.113297
Peroxisomes 0.003448 0.013053 0.000203 0.000847 0.002715 0.011389 0.000319 0.001356 0.003102 0.012009 0.000256 0.000921
Vacuole 0.077615 0.121148 0.041560 0.130585 0.084474 0.118925 0.076034 0.269133* 0.080849 0.119753 0.057390 0.150932
Vacuole Periphery 0.002082 0.003178 0.000122 0.003315 0.001481 0.003543 0.000090 0.005846 0.001798 0.003407 0.000107 0.003686

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.09 -2.84 -3.01 0.42 -1.01 -4.15 -2.78 -1.75 2.37 -0.95 -5.74 -3.25 -3.23 -0.02 -1.76
Bud Neck 2.04 15.91 13.41 10.04 -1.97 -11.27 9.97 2.71 9.79 -1.41 -4.93 18.44 12.38 16.77 -3.13
Bud Site -6.73 2.39 -1.67 3.32 -3.02 -12.01 2.28 -0.90 6.11 -1.63 -12.40 3.00 -2.93 4.31 -4.14
Cell Periphery 2.03 10.67 6.86 1.16 -1.38 0.75 9.26 3.42 1.84 -0.84 2.63 13.80 6.09 1.35 -2.51
Cytoplasm 10.91 5.79 2.01 -7.33 -2.58 12.97 -2.42 7.57 2.36 8.45 16.21 2.65 6.14 -5.35 4.16
Cytoplasmic Foci -5.50 52.56 46.84 37.57 -10.89 -4.49 44.75 35.29 38.09 -3.18 -6.62 68.91 60.77 56.63 -9.51
Eisosomes 8.35 14.48 13.11 9.67 -8.33 -0.18 6.52 4.02 6.08 -6.60 5.18 15.01 12.90 10.82 -10.31
Endoplasmic Reticulum 3.20 -6.92 -0.16 -6.13 9.65 4.88 -6.41 -2.21 -5.50 2.31 5.23 -8.96 -1.66 -10.21 9.20
Endosome -10.25 17.14 13.55 16.11 -2.37 -12.98 16.53 11.10 19.32 -2.83 -16.34 23.71 17.16 25.33 -2.79
Golgi -3.01 13.73 2.63 4.24 -2.05 -8.28 9.72 8.96 13.98 -0.78 -7.91 16.77 2.58 6.75 -2.05
Lipid Particles 4.05 12.17 11.99 4.49 -1.20 2.36 13.70 11.42 8.66 -1.93 5.28 16.72 16.28 9.46 -1.79
Mitochondria 1.28 7.00 5.29 4.47 -1.28 -4.19 2.82 0.65 3.95 -0.88 -2.65 7.26 4.40 6.11 -1.73
Mitotic Spindle -3.93 -3.37 -3.80 0.38 -1.78 -4.72 -3.61 -2.02 0.16 -0.28 -6.23 -4.87 -4.18 0.51 -1.23
None 5.41 2.50 1.82 -5.23 -0.71 6.28 -3.69 -1.55 -2.31 -0.36 6.89 0.41 -0.34 -6.93 -0.81
Nuclear Periphery 1.23 -23.64 -15.41 -19.04 11.37 -0.17 -20.45 -8.60 -7.41 -0.22 0.25 -31.13 -19.62 -13.43 9.57
Nuclear Periphery Foci -2.05 -10.56 -7.85 -6.42 2.52 5.76 -10.98 -3.74 -4.58 2.64 1.70 -15.14 -8.66 -9.13 3.48
Nucleolus 2.01 8.61 9.00 3.68 0.36 -1.84 7.96 4.53 4.33 -2.58 1.01 10.83 10.35 5.89 -1.59
Nucleus 12.55 -52.52 -16.60 -22.27 29.24 20.08 -27.21 -5.15 -9.05 7.34 23.83 -54.68 -17.27 -25.32 24.47
Peroxisomes -9.37 13.05 11.88 12.71 -2.14 -11.83 7.80 5.03 14.93 -1.05 -15.08 14.44 12.52 20.57 -2.54
Vacuole -9.81 1.04 -21.39 -14.17 -21.04 -8.18 -9.53 -14.15 -12.51 -11.40 -12.87 -6.70 -24.83 -18.89 -20.72
Vacuole Periphery -2.69 12.85 1.78 3.21 -2.10 -4.70 6.12 -0.17 1.15 -1.11 -5.26 12.58 1.11 3.88 -2.41
ORF
Human Ortholog
Description Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKR078W

YKR078W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr078w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available