Standard name
Human Ortholog
Description Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.05 0.07 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.99 0.94 0.94 0.95 0.9 0.83 0.84 0.84 0.84 0.84 1.0 0.97 0.95 0.95 0.98 0.99 0.95 0.95 0.86 0.84 0.9 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0.1 0.07 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 3 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 2 2 1 1 4 4 2 0 3 1 4 5 11 0 1 0 0 1 0 0 1 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 2 3 3 2 4 1 9 4 9 8 10 3 8 5 9 7 4 0 0 1 0 0 0
Cytoplasm 125 148 121 94 111 126 158 165 65 105 81 81 180 292 245 372 270 223 117 148 108 92 192 174
Endoplasmic Reticulum 1 0 1 1 2 0 5 13 8 9 5 5 0 3 3 12 7 5 0 1 1 1 2 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0
Golgi 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3 0 4 5 5 6 4 2 0 0 0 2 0 0 0 0 1 2 0 2
Nucleus 0 0 1 0 0 2 0 4 1 6 1 6 0 1 2 8 2 0 2 1 2 2 3 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 0 7 3 3 8 16 1 1 3 0 1 6 1 14 2 1 0 0 7 2 3 8
Unique Cell Count 129 148 122 100 118 132 176 199 77 125 97 96 180 300 257 392 275 226 123 157 126 110 213 207
Labelled Cell Count 134 150 128 107 122 136 181 218 89 137 105 108 188 315 267 418 289 233 123 157 126 110 213 207


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.2 22.5 22.6 22.4 26.2 27.1 25.7 24.4 25.0 21.8 24.7 24.3 32.6 38.4 39.3 36.7 47.1 48.2 18.7 23.6 29.3
Std Deviation (1e-4) 7.4 6.0 6.1 5.5 5.9 5.8 6.7 6.4 8.5 6.8 6.6 5.5 11.6 12.9 14.1 9.9 13.6 17.2 7.3 7.6 12.3
Intensity Change (Log2) -0.01 0.22 0.26 0.19 0.11 0.14 -0.05 0.13 0.11 0.53 0.77 0.8 0.7 1.06 1.09 -0.27 0.06 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1.7 0 2.5 0 0 0 0 0 0
Cytoplasm -2.2 -2.2 -1.8 -3.3 -4.5 -4.1 -4.3 -4.3 -4.2 1.2 -1.2 -1.9 -2.1 -0.8 -0.4
Endoplasmic Reticulum 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.2 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.4823 20.2919 19.6388 17.51 16.9083 17.9331 17.6971 23.9426 21.7452 19.3114 17.558 19.3791 14.8495 21.4918 20.7739 17.8628 19.8526 18.7459
Actin 0.0141 0.0005 0.0002 0.0002 0.0029 0.0012 0.0178 0.0004 0.0052 0.002 0.015 0.0022 0.0586 0.0002 0.0076 0.0932 0.0042 0.0023
Bud 0.0003 0.0025 0.0001 0.0001 0.0008 0.0001 0.0004 0.0002 0.0004 0.0006 0.0005 0.0021 0.0016 0.0001 0.0003 0.001 0.0001 0.0016
Bud Neck 0.0006 0.0002 0.0002 0.0004 0.0006 0.0009 0.001 0.0002 0.0007 0.0007 0.0007 0.0011 0.0151 0.0002 0.0003 0.0003 0.0006 0.0008
Bud Periphery 0.0002 0.0013 0 0 0.0011 0 0.0004 0 0.0003 0.0004 0.0003 0.0026 0.0019 0 0.0004 0.0013 0 0.0004
Bud Site 0.0003 0.0014 0 0.0001 0.0002 0.0002 0.0043 0.0003 0.0117 0.0056 0.0008 0.0002 0.0352 0.0001 0.001 0.0015 0.0001 0.0002
Cell Periphery 0.0001 0.0001 0 0 0.0006 0 0.0002 0 0.0002 0.0003 0.0001 0.0001 0.0009 0 0.0002 0 0 0.0001
Cytoplasm 0.8825 0.9806 0.9764 0.9742 0.9595 0.9116 0.8583 0.9865 0.9646 0.87 0.8253 0.9327 0.6822 0.9897 0.8789 0.8756 0.9027 0.9022
Cytoplasmic Foci 0.0132 0.0004 0.0006 0.0004 0.001 0.0025 0.0226 0.001 0.0013 0.0152 0.0136 0.0072 0.0238 0.0006 0.0107 0.0018 0.0069 0.0099
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0004 0 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.0047 0.0005 0.0005 0.0003 0.0049 0.0022 0.0036 0.0005 0.0009 0.0043 0.0089 0.0019 0.0187 0.0002 0.0034 0.0002 0.0011 0.0054
Endosome 0.0105 0.0001 0.0004 0.0001 0.0007 0.0037 0.0149 0.0008 0.0008 0.0235 0.0311 0.002 0.0306 0.0001 0.0107 0.0006 0.0021 0.0088
Golgi 0.0031 0 0 0 0.0001 0.0003 0.0094 0.0001 0.0002 0.0116 0.0175 0.0014 0.017 0 0.0022 0.0048 0.0022 0.0009
Lipid Particles 0.0057 0 0 0 0.0001 0.0002 0.0078 0 0.0002 0.0162 0.0061 0.0001 0.0181 0 0.0082 0.0006 0.0196 0.0014
Mitochondria 0.001 0.0001 0.0001 0.0001 0.0001 0.0004 0.015 0.0001 0.0002 0.0234 0.0083 0.0055 0.0089 0.0001 0.0225 0.0021 0.0017 0.005
None 0.0125 0.0005 0.0003 0.0004 0.0006 0.0013 0.0032 0.0006 0.0011 0.001 0.0011 0.0028 0.007 0.0006 0.001 0.0003 0.0012 0.0014
Nuclear Periphery 0.0136 0.0016 0.0022 0.0018 0.0067 0.0087 0.0048 0.0008 0.0011 0.0042 0.0159 0.0036 0.0255 0.0008 0.0017 0.001 0.0027 0.008
Nucleolus 0.0005 0 0 0 0 0.0007 0.0008 0 0.0001 0.0001 0.0002 0.0002 0.0007 0 0.0002 0 0 0.0001
Nucleus 0.0219 0.0094 0.0184 0.0214 0.0177 0.054 0.0218 0.0077 0.0095 0.0136 0.0226 0.0184 0.0229 0.007 0.0139 0.0108 0.0188 0.018
Peroxisomes 0.007 0 0 0 0 0.0001 0.007 0 0.0002 0.0015 0.0275 0.0002 0.0195 0 0.0022 0.0042 0.0347 0.0005
Punctate Nuclear 0.0062 0.0002 0.0002 0.0003 0.0011 0.0107 0.0044 0.0003 0.0003 0.0027 0.0015 0.0147 0.0049 0.0002 0.0333 0.0002 0.0009 0.0307
Vacuole 0.001 0.0004 0.0003 0.0002 0.0011 0.0009 0.0015 0.0003 0.0009 0.0022 0.0022 0.0007 0.0033 0.0001 0.0009 0.0001 0.0002 0.0015
Vacuole Periphery 0.0006 0.0001 0.0001 0.0001 0.0002 0.0003 0.0006 0.0001 0.0001 0.0007 0.0008 0.0002 0.0032 0 0.0003 0.0001 0.0001 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.9014 18.7287 22.133 27.885 32.6968 28.6943 23.1446 24.1417 21.8677 37.2519
Translational Efficiency 0.6838 0.7434 0.5416 0.6558 0.7238 1.0973 0.7417 0.5972 0.9088 0.7722

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2001 1303 122 1430 1650 1646 425 131 3651 2949 547 1561

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1706.19 1862.09 2330.18 1844.12 2027.57 1754.64 2392.84 2425.10 1851.43 1802.12 2378.86 1892.88
Standard Deviation 320.25 476.17 511.52 428.99 451.64 365.23 437.55 640.41 417.10 421.29 455.84 478.49
Intensity Change Log 2 0.126144 0.449663 0.112154 -0.208577 0.238972 0.258293 -0.045943 0.339080 0.193343

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000104 0.000226 0.002648 0.000722 0.000185 0.000260 0.000268 0.000782 0.000140 0.000245 0.000799 0.000727
Bud Neck 0.000200 0.000366 0.004603 0.003561 0.000410 0.000401 0.001434 0.002010 0.000295 0.000385 0.002141 0.003431
Bud Site 0.001374 0.001887 0.004601 0.020169 0.000881 0.002754 0.001653 0.006463 0.001151 0.002371 0.002311 0.019019
Cell Periphery 0.000056 0.000105 0.001104 0.000296 0.000145 0.000061 0.000320 0.000335 0.000097 0.000081 0.000495 0.000299
Cytoplasm 0.809562* 0.759341* 0.377822* 0.673471* 0.696961* 0.822143* 0.567204* 0.599699* 0.758674* 0.794395* 0.524965* 0.667280*
Cytoplasmic Foci 0.003432 0.003458 0.017075 0.008882 0.002594 0.007318 0.007773 0.064531 0.003053 0.005612 0.009848 0.013552
Eisosomes 0.000001 0.000002 0.000011 0.000008 0.000001 0.000002 0.000003 0.000019 0.000001 0.000002 0.000004 0.000009
Endoplasmic Reticulum 0.000165 0.000305 0.000923 0.001516 0.000131 0.000116 0.000182 0.000102 0.000150 0.000200 0.000347 0.001398
Endosome 0.000210 0.000410 0.000615 0.005801 0.000052 0.000865 0.000249 0.007939 0.000139 0.000664 0.000331 0.005980
Golgi 0.000348 0.001026 0.001039 0.008436 0.000344 0.001943 0.000346 0.063236 0.000346 0.001538 0.000500 0.013035
Lipid Particles 0.000083 0.000258 0.002265 0.000761 0.000141 0.000604 0.000791 0.001791 0.000109 0.000451 0.001120 0.000848
Mitochondria 0.000206 0.000222 0.000603 0.008836 0.000233 0.000285 0.000256 0.013406 0.000218 0.000257 0.000333 0.009219
Mitotic Spindle 0.001189 0.001177 0.001886 0.054485 0.000347 0.007204 0.004291 0.002579 0.000808 0.004541 0.003754 0.050129
None 0.022346 0.018248 0.005664 0.009463 0.013253 0.018564 0.012826 0.015818 0.018237 0.018424 0.011228 0.009996
Nuclear Periphery 0.000033 0.000113 0.000685 0.000318 0.000052 0.000055 0.000452 0.000175 0.000042 0.000081 0.000504 0.000306
Nuclear Periphery Foci 0.000947 0.001816 0.003520 0.001675 0.000653 0.001340 0.001948 0.002251 0.000814 0.001550 0.002299 0.001724
Nucleolus 0.000069 0.000361 0.000194 0.000220 0.000065 0.000101 0.000118 0.000058 0.000067 0.000216 0.000135 0.000207
Nucleus 0.158102* 0.204837* 0.541133* 0.153079* 0.281587* 0.130799* 0.380730* 0.158532* 0.213909* 0.163513* 0.416505* 0.153536*
Peroxisomes 0.000061 0.000090 0.000128 0.000291 0.000009 0.000374 0.000025 0.001194 0.000037 0.000249 0.000048 0.000367
Vacuole 0.001301 0.004754 0.033258 0.041605 0.001760 0.004196 0.019053 0.058166 0.001509 0.004443 0.022221 0.042994
Vacuole Periphery 0.000210 0.000996 0.000224 0.006404 0.000195 0.000617 0.000077 0.000914 0.000203 0.000784 0.000110 0.005944

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.85 -1.57 -4.47 -0.76 1.52 -0.93 -5.79 -1.79 -0.52 3.13 -2.53 -1.94 -3.86 -0.70 1.75
Bud Neck -5.67 -8.35 -12.93 -10.81 6.27 0.14 -9.02 -4.93 -4.95 -0.60 -3.49 -12.18 -13.11 -12.09 5.95
Bud Site -1.19 -3.66 -5.95 -5.03 0.95 -2.56 -4.70 -9.62 -6.54 -4.02 -2.67 -5.74 -7.10 -5.30 -1.17
Cell Periphery -4.67 -9.83 -6.64 -3.03 9.16 6.09 -8.25 -4.57 -5.59 0.45 1.25 -12.41 -5.77 -6.30 10.31
Cytoplasm 9.19 27.57 22.75 12.71 -17.94 -18.56 18.24 6.27 11.54 -2.46 -6.84 31.57 16.94 21.47 -17.79
Cytoplasmic Foci -1.61 -7.69 -10.05 -6.37 6.22 -6.48 -9.86 -8.41 -7.03 -4.56 -6.08 -12.03 -13.23 -6.95 5.83
Eisosomes -2.65 -7.82 -14.11 -5.88 5.98 -3.38 -10.72 -7.32 -6.21 -1.92 -4.24 -12.35 -16.22 -9.13 5.66
Endoplasmic Reticulum -1.03 -4.67 -1.06 -0.20 3.30 0.67 -3.84 1.87 1.22 5.79 -0.69 -4.55 -1.19 -0.85 1.04
Endosome -1.09 -4.73 -3.40 -2.65 0.09 -3.55 -8.31 -1.96 -0.32 -0.88 -3.24 -5.90 -4.11 -2.04 -1.95
Golgi -1.67 -6.92 -2.01 -0.74 0.32 -2.61 -3.12 -0.97 -0.61 -0.92 -3.09 -5.62 -2.22 -0.40 -1.49
Lipid Particles -2.03 -6.98 -13.54 -7.36 5.43 -1.24 -8.58 -5.61 -4.69 -1.92 -1.82 -11.03 -13.57 -5.73 5.73
Mitochondria -0.07 -3.16 -1.57 -1.57 -1.00 -0.12 -2.73 -0.77 -0.74 -0.67 -0.16 -2.52 -1.63 -1.60 -1.39
Mitotic Spindle 0.12 -2.26 -6.66 -6.76 -5.79 -4.46 -4.55 -3.08 0.95 2.52 -3.99 -4.56 -7.00 -5.40 -2.49
None 3.56 16.20 14.21 8.33 -4.18 -3.72 4.33 6.56 8.39 3.39 1.17 12.27 15.87 12.63 2.44
Nuclear Periphery -2.10 -10.72 -22.98 -8.72 6.94 -2.17 -15.37 -10.60 -10.33 6.29 -2.59 -18.71 -24.17 -16.08 10.18
Nuclear Periphery Foci -4.54 -6.84 -5.46 1.55 5.92 -5.38 -8.55 -4.89 -3.36 -0.29 -6.71 -10.59 -7.98 -0.24 7.00
Nucleolus -1.89 -9.44 -7.92 1.00 2.50 -2.64 -12.30 -3.66 0.06 6.13 -2.22 -14.84 -8.60 0.32 0.69
Nucleus -8.62 -15.41 -0.48 7.67 15.20 24.26 -7.01 16.08 3.62 18.62 11.27 -18.00 12.45 2.77 23.81
Peroxisomes -0.58 -1.78 -0.87 0.02 1.54 -1.89 -2.59 -2.25 0.75 -1.24 -1.91 -2.38 -2.64 1.32 0.74
Vacuole -7.15 -8.21 -25.93 -24.29 -1.73 -7.62 -12.51 -12.03 -11.81 -7.56 -10.64 -14.90 -27.92 -26.70 -5.51
Vacuole Periphery -1.43 -0.81 -1.99 -0.23 -1.83 -1.15 1.96 1.19 1.39 0.05 -1.81 0.63 -2.04 -0.58 -2.18
Standard name
Human Ortholog
Description Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Tub4

Tub4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tub4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available