ORF
Human Ortholog
Description Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 1.0 0.96 0.98 1.0 0.97 0.9 0.79 0.75 0.73 0.69 0.58 0.65 1.0 0.99 0.99 0.94 0.94 0.9 0.94 0.92 0.94 0.93 0.96 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.1 0.07 0.15 0.26 0.22 0.35 0.38 0.48 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.1 0.05 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 2 4 0 1 2 2 3 4 6 3 3 1 0 0 2 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 1 2 3 1 4 9 12 19 3 11 4 4 10 11 0 0 0 0 0 0
Cytoplasm 41 260 180 69 117 209 204 239 151 174 176 193 204 383 368 357 276 176 61 253 178 147 217 182
Endoplasmic Reticulum 0 1 0 0 0 1 3 2 2 3 1 7 0 1 4 9 4 6 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 6 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 9 7 7 8 35 68 70 72 96 146 122 0 1 0 10 0 1 0 4 1 0 0 1
Nucleus 0 1 1 0 0 3 0 3 1 3 7 2 0 0 2 17 20 21 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 3 0 2 7 2 1 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 0 3 1 0 2 2 12 32 10 18 25 7 0 0 0 5 2 2 0 3 3 2 0 3
Unique Cell Count 41 272 183 69 121 231 258 317 206 251 303 299 204 385 373 378 295 195 65 277 189 158 227 198
Labelled Cell Count 43 278 193 76 129 254 295 350 246 316 373 356 208 396 378 405 314 218 65 277 189 158 227 198


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.2 5.2 5.3 5.8 5.8 5.2 5.8 5.8 5.3 5.5 5.3 7.2 7.9 8.0 8.5 10.7 10.9 6.0 6.5 7.7
Std Deviation (1e-4) 0.9 0.9 0.7 1.0 1.0 1.2 1.1 1.3 1.6 1.2 1.4 1.2 1.3 1.1 1.1 1.7 2.0 1.9 1.4 1.3 1.6
Intensity Change (Log2) – – – 0.01 0.16 0.15 0.0 0.15 0.16 0.01 0.07 0.03 0.47 0.59 0.62 0.71 1.03 1.06 0.2 0.31 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 3.3
Cytoplasm 1.1 -0.9 -3.3 -5.9 -6.7 -6.9 -7.7 -9.7 -8.6 1.8 1.3 0.3 -2.1 -2.4 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.1 3.8 6.2 5.4 7.6 8.3 10.2 8.9 0 0 0 -0.8 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 2.5 3.2 4.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 4.1 2.6 3.3 3.7 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3483 3.065 2.9091 2.5069 2.4633 2.9858 3.5607 3.8259 3.641 2.9951 3.0254 3.6964 3.4472 3.918 3.6496 3.2943 3.8765 3.5499
Actin 0.0042 0.0011 0.0005 0.0006 0.0209 0.0011 0.0331 0.0004 0.0141 0.0022 0.0034 0.0083 0.042 0.0007 0.0035 0.0236 0.0005 0.0005
Bud 0.0007 0.0001 0.0001 0.0002 0.0002 0.0003 0.0019 0.0002 0.0011 0.0004 0.001 0.0007 0.001 0.0002 0.0008 0.0004 0 0
Bud Neck 0.0028 0.0001 0.0002 0.0044 0.0375 0.0007 0.0022 0.0001 0.0005 0.0004 0.0005 0.0034 0.0019 0.0001 0.0001 0.0007 0.0002 0.0003
Bud Periphery 0.001 0.0001 0.0001 0.0003 0.0004 0.0003 0.0058 0.0001 0.0011 0.0004 0.0021 0.0009 0.0009 0.0001 0.0007 0.0006 0 0
Bud Site 0.0089 0.0004 0.0003 0.0023 0.0021 0.0001 0.0108 0.0008 0.0065 0.0004 0.0055 0.0066 0.0484 0.0016 0.0013 0.0006 0.0001 0
Cell Periphery 0.0013 0.0002 0.0002 0.0011 0.0027 0.0001 0.001 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.522 0.8605 0.8048 0.8164 0.803 0.8458 0.6454 0.8258 0.7475 0.686 0.6327 0.7654 0.6603 0.7898 0.8089 0.7966 0.783 0.7675
Cytoplasmic Foci 0.0325 0.0032 0.0024 0.0282 0.0146 0.0052 0.0315 0.0048 0.0301 0.0154 0.0568 0.0131 0.0262 0.0182 0.0102 0.0108 0.0062 0.0098
Eisosomes 0.0001 0 0 0.0001 0.0045 0 0.0007 0.0001 0.0001 0 0.0001 0 0.0005 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0041 0.004 0.005 0.0075 0.0115 0.0047 0.006 0.0027 0.0037 0.0012 0.0034 0.0019 0.0154 0.0033 0.0038 0.0072 0.0044 0.0021
Endosome 0.0913 0.0009 0.0009 0.0135 0.0072 0.0039 0.0267 0.0031 0.0241 0.0192 0.033 0.0059 0.0566 0.0028 0.003 0.0044 0.0035 0.0014
Golgi 0.0634 0.0001 0.0002 0.0014 0.0057 0.0004 0.0043 0.0003 0.0135 0.0178 0.0213 0.0019 0.012 0.0007 0.0021 0.0044 0.0002 0.0003
Lipid Particles 0.0179 0.0001 0.0002 0.0062 0.0093 0.0001 0.0052 0.0002 0.0045 0.0008 0.0121 0.0008 0.0311 0.0014 0.0008 0.006 0.0003 0.0019
Mitochondria 0.0141 0.0002 0.0242 0.0007 0.0006 0.0009 0.0074 0.0001 0.0162 0.0059 0.036 0.0003 0.0035 0.0001 0.0164 0.0008 0.0001 0.0001
None 0.1915 0.1235 0.1581 0.1056 0.0679 0.1293 0.1894 0.1569 0.1138 0.2447 0.1239 0.1813 0.0693 0.1724 0.1409 0.1235 0.197 0.2073
Nuclear Periphery 0.0176 0.0018 0.0006 0.0011 0.0007 0.0011 0.0054 0.0005 0.0014 0.0005 0.0031 0.0004 0.0101 0.0011 0.0009 0.0016 0.0012 0.0004
Nucleolus 0.0009 0 0 0.0005 0.0039 0.0001 0.0004 0 0.0001 0 0.0002 0 0.0003 0.0001 0 0.0002 0 0
Nucleus 0.0028 0.0022 0.0011 0.0018 0.0022 0.004 0.0049 0.0021 0.0022 0.0014 0.005 0.0016 0.0023 0.0017 0.0011 0.0041 0.0017 0.0019
Peroxisomes 0.0077 0.0001 0 0.001 0.001 0.0002 0.01 0.0001 0.0161 0.0017 0.0514 0.0058 0.0084 0.0007 0.0041 0.0114 0.0001 0.0047
Punctate Nuclear 0.0032 0.0003 0.0002 0.0008 0.0026 0.0007 0.0032 0.0005 0.0012 0.0004 0.0052 0.0012 0.0036 0.0042 0.0006 0.0012 0.001 0.0013
Vacuole 0.0065 0.0008 0.0007 0.006 0.0012 0.001 0.0033 0.0011 0.0014 0.0008 0.0022 0.0003 0.0042 0.0007 0.0005 0.0014 0.0005 0.0003
Vacuole Periphery 0.0055 0.0001 0.0001 0.0004 0.0002 0.0002 0.0014 0.0001 0.0007 0.0003 0.0008 0.0001 0.0015 0.0001 0.0001 0.0003 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2118 7.7363 5.7407 13.5738 7.0128 8.6494 14.9925 15.3983 15.9608 12.576
Translational Efficiency 0.7893 0.7349 1.1828 0.7117 0.7975 1.3774 0.842 0.7596 1.0353 0.9332

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1950 597 766 1677 2113 184 324 883 4063 781 1090 2560

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 773.78 829.15 1195.27 1036.20 800.77 832.21 1212.74 1036.97 787.82 829.87 1200.46 1036.47
Standard Deviation 83.71 168.92 159.14 136.83 96.65 121.20 153.39 145.13 91.67 158.98 157.66 139.75
Intensity Change Log 2 — 0.099710 0.627341 0.421307 — 0.055560 0.598810 0.372914 — 0.077425 0.612902 0.396899

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000654 0.000354 0.002170 0.000119 0.001204 0.000255 0.002436 0.000104 0.000783 0.000325 0.002262
Bud Neck 0.006898 0.020602 0.001740 0.006234 0.009640 0.021565 0.001163 0.004167 0.008324 0.020829 0.001568 0.005521
Bud Site 0.006274 0.036581 0.008430 0.030883 0.007179 0.033874 0.004627 0.032987 0.006745 0.035943 0.007300 0.031609
Cell Periphery 0.000087 0.000148 0.000096 0.000121 0.000076 0.000114 0.000105 0.000098 0.000081 0.000140 0.000098 0.000113
Cytoplasm 0.681834* 0.452709* 0.685380* 0.646963* 0.641451* 0.415636* 0.641976* 0.653652* 0.660833* 0.443975* 0.672478* 0.649271*
Cytoplasmic Foci 0.120364 0.254539* 0.012305 0.028915 0.150754 0.254937* 0.024414 0.031250 0.136169 0.254633* 0.015904 0.029720
Eisosomes 0.000059 0.000109 0.000014 0.000019 0.000060 0.000106 0.000027 0.000021 0.000059 0.000108 0.000018 0.000019
Endoplasmic Reticulum 0.000636 0.000844 0.001542 0.000554 0.000682 0.000587 0.001236 0.000843 0.000660 0.000783 0.001451 0.000653
Endosome 0.006410 0.041756 0.002450 0.008997 0.009910 0.044645 0.003735 0.006682 0.008230 0.042436 0.002832 0.008198
Golgi 0.001478 0.009941 0.000186 0.006209 0.001954 0.006418 0.000141 0.004713 0.001725 0.009111 0.000173 0.005693
Lipid Particles 0.001694 0.003356 0.000918 0.000688 0.002297 0.002460 0.001077 0.001875 0.002008 0.003145 0.000965 0.001097
Mitochondria 0.001743 0.004446 0.000316 0.001834 0.003002 0.009451 0.000084 0.000549 0.002398 0.005625 0.000247 0.001391
Mitotic Spindle 0.001819 0.003563 0.011056 0.077580 0.001947 0.014389 0.002459 0.029538 0.001885 0.006113 0.008500 0.061009
None 0.005202 0.001414 0.004656 0.002350 0.002366 0.000739 0.004715 0.004481 0.003727 0.001255 0.004673 0.003085
Nuclear Periphery 0.000153 0.000163 0.000904 0.000532 0.000189 0.000196 0.000586 0.001520 0.000172 0.000171 0.000810 0.000873
Nuclear Periphery Foci 0.000402 0.000877 0.004647 0.001555 0.000495 0.000658 0.005785 0.006381 0.000450 0.000825 0.004985 0.003219
Nucleolus 0.000560 0.000950 0.000139 0.000228 0.000433 0.000867 0.000319 0.000187 0.000494 0.000930 0.000193 0.000213
Nucleus 0.085995 0.030652 0.188946* 0.054627 0.089893 0.029296 0.072248 0.048647 0.088022 0.030333 0.154258* 0.052564
Peroxisomes 0.001218 0.015843 0.000199 0.005193 0.001765 0.024176 0.000166 0.002194 0.001502 0.017806 0.000189 0.004159
Vacuole 0.076266 0.118407 0.075509 0.119353 0.074936 0.135533 0.234727* 0.165704 0.075574 0.122442 0.122836 0.135340
Vacuole Periphery 0.000820 0.002447 0.000214 0.004995 0.000853 0.003149 0.000156 0.002077 0.000837 0.002612 0.000197 0.003988

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.58 -10.78 -3.85 -0.81 -1.75 -1.68 -5.12 -1.73 0.52 -1.32 -4.24 -11.54 -3.86 -0.14 -2.11
Bud Neck -6.38 9.57 6.26 8.34 -1.82 -3.15 9.50 7.76 5.40 -1.20 -6.61 12.56 9.61 9.96 -2.08
Bud Site -9.23 -0.59 -5.81 6.38 -3.55 -4.39 1.69 -2.79 3.15 -3.72 -10.10 -0.00 -6.03 7.13 -4.71
Cell Periphery -4.26 -5.08 1.22 4.17 3.68 -1.67 -1.41 1.94 2.31 2.61 -4.82 -5.48 1.58 5.02 4.59
Cytoplasm 17.89 10.11 13.82 -8.45 1.00 10.22 8.99 7.98 -5.75 -3.15 19.66 12.28 14.81 -10.01 -0.88
Cytoplasmic Foci -14.83 33.27 29.50 27.48 -7.33 -6.47 33.02 32.64 15.11 -1.46 -15.09 48.11 44.21 31.36 -6.89
Eisosomes -5.96 13.93 14.38 10.85 -0.32 -3.37 9.95 15.64 6.19 4.20 -6.86 18.56 21.45 12.47 2.04
Endoplasmic Reticulum -1.25 -7.96 1.15 3.04 11.70 0.69 -4.17 1.74 0.66 5.17 -1.15 -9.69 2.16 3.03 12.64
Endosome -9.69 7.36 6.10 10.75 0.35 -5.22 8.95 8.52 6.38 0.69 -10.66 11.51 10.33 12.51 0.60
Golgi -4.79 7.37 0.18 4.70 -2.69 -2.06 5.97 1.13 2.36 -1.88 -5.10 8.64 0.99 5.25 -3.28
Lipid Particles -3.51 1.40 4.97 5.57 6.69 -0.34 4.93 4.57 2.52 1.12 -2.96 4.42 7.39 6.01 4.89
Mitochondria -2.75 3.02 2.44 4.61 -2.10 -1.04 3.76 3.60 1.53 -1.65 -1.96 4.66 4.19 3.37 -2.35
Mitotic Spindle -1.59 -3.50 -9.10 -8.56 -5.16 -1.84 -2.24 -5.14 -0.52 -3.85 -2.38 -3.95 -10.46 -7.22 -6.06
None 4.76 2.05 4.95 -1.74 4.30 3.72 -1.19 -1.07 -4.69 0.55 4.47 -0.07 3.27 -2.80 3.36
Nuclear Periphery -0.55 -19.12 -13.62 -15.63 10.65 -0.51 -10.36 -2.69 -2.61 0.26 -0.31 -22.02 -6.41 -6.35 5.09
Nuclear Periphery Foci -4.15 -13.11 -8.48 -3.06 10.80 -1.60 -10.05 -2.65 -2.34 2.82 -4.29 -16.03 -4.74 -3.10 9.33
Nucleolus -1.50 6.87 6.39 2.97 -1.33 -1.82 3.20 5.59 3.12 0.33 -2.08 7.98 8.49 3.65 -0.32
Nucleus 18.76 -18.65 0.66 -16.38 18.63 15.84 0.77 6.99 -7.99 3.58 24.96 -16.31 4.48 -17.86 17.94
Peroxisomes -8.83 7.83 -0.33 8.51 -2.95 -4.27 11.12 4.56 4.48 -2.99 -9.24 13.32 1.29 9.36 -3.57
Vacuole -7.34 -10.33 -28.29 -15.73 -11.73 -5.58 -17.93 -23.76 -10.32 3.10 -9.23 -18.29 -36.93 -19.42 -6.77
Vacuole Periphery -5.74 6.86 -0.78 2.47 -2.26 -2.38 5.96 0.89 2.58 -1.58 -5.67 8.55 -0.40 3.82 -2.65
ORF
Human Ortholog
Description Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLR345W

YLR345W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr345w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available