Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.98 0.98 0.97 0.94 0.89 – 0.8 0.79 0.67 0.71 0.99 0.99 0.99 – 0.97 0.94 0.98 0.93 0.87 0.92 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.1 0.14 – 0.22 0.25 0.45 0.3 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 4 0 0 1 – 0 0 0 0 3 0 0 0
Bud 0 2 1 0 2 3 2 – 1 4 8 2 1 0 1 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 – 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 5 4 1 9 6 11 – 6 15 8 14 0 1 6 – 32 32 0 0 1 0 0 0
Cytoplasm 90 150 196 131 284 302 447 – 243 286 218 247 170 212 270 – 347 331 90 144 187 74 176 177
Endoplasmic Reticulum 1 2 0 0 1 0 3 – 0 0 2 20 0 0 2 – 13 14 0 0 3 0 0 0
Endosome 0 0 1 0 0 1 1 – 3 4 5 2 0 1 0 – 0 4 0 0 2 1 0 0
Golgi 0 2 0 0 1 0 1 – 2 0 0 0 0 0 0 – 3 2 0 1 0 0 0 1
Mitochondria 3 1 0 8 8 32 71 – 67 90 147 103 0 0 0 – 3 6 0 1 6 0 3 2
Nucleus 0 0 1 4 1 0 1 – 4 1 1 5 1 1 1 – 1 4 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 1 – 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 1 – 0 1 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 0 5 3 7 – 3 21 5 7 0 2 1 – 7 10 0 2 3 2 0 2
Unique Cell Count 91 154 201 133 294 322 501 302 362 326 346 171 215 273 358 351 92 155 215 81 185 190
Labelled Cell Count 95 162 207 144 312 348 546 330 422 398 405 172 217 282 406 404 92 155 215 81 185 190


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.5 7.0 6.4 7.2 5.9 6.2 – 5.5 5.7 5.1 5.4 7.0 7.2 6.8 – 8.6 8.1 5.5 6.5 7.0
Std Deviation (1e-4) 0.6 1.1 1.4 1.1 1.4 1.3 1.1 – 1.4 1.3 1.3 1.0 0.7 1.2 0.9 – 1.5 1.4 0.5 0.9 1.1
Intensity Change (Log2) – – – -0.12 0.03 -0.24 -0.18 – -0.35 -0.29 -0.46 -0.38 -0.0 0.04 -0.04 – 0.29 0.21 -0.35 -0.11 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0.7 0 0 – 0 1.4 0 1.3 0 0 0 – 3.2 3.3
Cytoplasm 0.6 -0.6 -1.9 -3.6 – -5.6 -6.0 -8.3 -7.5 1.5 0.8 1.2 – -0.4 -1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 3.5 0 0 0 – 0 2.9
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 4.6 5.6 – 7.2 7.7 11.2 8.6 0 0 0 – 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 – 0 2.4 0 0 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1539 0.4065 0.1792 -0.0851 -0.2018 -0.1677 0.4052 1.4019 1.2065 1.4339 0.3508 1.1567 0.3634 1.9958 1.1459 1.2657 1.2436 1.1177
Actin 0.0395 0.0222 0.0119 0.0015 0.0043 0.0259 0.0041 0.0196 0.0133 0.0047 0.0476 0.0031 0.0142 0.0005 0.0033 0.0016 0.0012 0.0045
Bud 0.0004 0.0007 0.0016 0.0005 0.0003 0.0003 0.0171 0.0393 0.0325 0.0116 0.0083 0.0036 0.0004 0.004 0.0011 0.0014 0.0002 0.0002
Bud Neck 0.0025 0.0008 0.0008 0.0012 0.0004 0.0009 0.0008 0.0003 0.001 0.0471 0.0004 0.0016 0.0004 0.0003 0.0008 0.001 0.0003 0.0017
Bud Periphery 0.0005 0.0007 0.0021 0.0003 0.0003 0.0003 0.0084 0.0095 0.0132 0.013 0.0118 0.0089 0.0005 0.0019 0.0004 0.0024 0.0001 0.0002
Bud Site 0.0031 0.0121 0.0051 0.0147 0.0003 0.0004 0.0038 0.0535 0.0183 0.0775 0.0162 0.0016 0.001 0.0084 0.0015 0.0015 0.0004 0.0001
Cell Periphery 0.0005 0.0013 0.0004 0.0002 0.0001 0.0001 0.0006 0.0014 0.0007 0.0017 0.0003 0.0003 0.0003 0.0003 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.4442 0.5376 0.6147 0.6144 0.5585 0.6698 0.419 0.5534 0.5588 0.3983 0.4453 0.618 0.6952 0.8363 0.785 0.7416 0.8909 0.8391
Cytoplasmic Foci 0.0588 0.0161 0.017 0.0422 0.0303 0.0152 0.0424 0.0053 0.0157 0.0578 0.0218 0.014 0.023 0.0191 0.0262 0.0213 0.0177 0.0171
Eisosomes 0.0005 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0005 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0062 0.0068 0.0057 0.0036 0.0016 0.0022 0.0043 0.0021 0.0025 0.0015 0.0022 0.0017 0.0074 0.0047 0.0052 0.0069 0.0065 0.0073
Endosome 0.0369 0.0109 0.0184 0.0462 0.0392 0.0081 0.0181 0.0015 0.0062 0.0342 0.0135 0.0052 0.0156 0.0191 0.0448 0.0442 0.0317 0.0228
Golgi 0.0128 0.0089 0.0065 0.0124 0.0321 0.0061 0.0051 0.0003 0.0045 0.0143 0.0172 0.0036 0.0035 0.0018 0.0042 0.002 0.0016 0.0011
Lipid Particles 0.0174 0.0051 0.0069 0.0032 0.0263 0.0137 0.0109 0.0002 0.0016 0.0039 0.0073 0.0043 0.009 0.0005 0.0034 0.0018 0.0004 0.0002
Mitochondria 0.0103 0.0026 0.0075 0.0486 0.0039 0.0117 0.0038 0.0005 0.0028 0.0213 0.0191 0.0103 0.016 0.0004 0.0079 0.0049 0.0004 0.006
None 0.3252 0.3614 0.2883 0.1956 0.285 0.2329 0.4228 0.3069 0.3055 0.2187 0.3524 0.2993 0.1775 0.0947 0.0909 0.1248 0.0375 0.0916
Nuclear Periphery 0.0035 0.0016 0.0017 0.0011 0.0004 0.0008 0.0098 0.0006 0.0016 0.0026 0.0007 0.0007 0.004 0.0008 0.0018 0.0039 0.0014 0.0013
Nucleolus 0.0012 0.0002 0.0011 0.0003 0.0001 0.0001 0.0006 0.0003 0.001 0.0093 0.0003 0.0027 0.001 0.0002 0.0009 0.0007 0.0001 0.0001
Nucleus 0.0023 0.001 0.0011 0.0011 0.0004 0.0007 0.002 0.0022 0.0043 0.0204 0.001 0.0017 0.0036 0.0015 0.0029 0.0061 0.0021 0.0023
Peroxisomes 0.022 0.0058 0.0033 0.0039 0.0139 0.0073 0.0143 0.0003 0.0064 0.0088 0.0303 0.0129 0.0129 0.0002 0.0028 0.0015 0.0004 0.0002
Punctate Nuclear 0.0045 0.0008 0.0022 0.001 0.0004 0.0017 0.0068 0.0007 0.0069 0.0349 0.0009 0.004 0.0108 0.0011 0.0063 0.0069 0.0014 0.0016
Vacuole 0.0065 0.0026 0.003 0.0068 0.0017 0.0013 0.0036 0.0019 0.0026 0.0158 0.0024 0.0017 0.0027 0.0037 0.0072 0.0206 0.0049 0.002
Vacuole Periphery 0.0012 0.0004 0.0006 0.0012 0.0005 0.0004 0.0016 0.0002 0.0004 0.0027 0.0006 0.0005 0.0006 0.0004 0.0032 0.0046 0.0006 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.1849 15.1901 11.2717 17.1374 19.5439 9.861 18.5791 16.0507 16.6694 18.6216
Translational Efficiency 0.6703 0.7578 0.8532 0.6428 0.5088 1.669 0.7383 0.7517 0.8202 0.6512

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1894 161 778 1253 2472 1992 187 271 4366 2153 965 1524

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.76 832.94 970.31 885.47 795.45 780.89 1009.64 977.24 753.94 784.78 977.93 901.79
Standard Deviation 80.83 118.95 107.86 129.08 93.52 100.04 103.45 115.73 100.18 102.49 108.14 131.58
Intensity Change Log 2 — 0.251352 0.471586 0.339583 — -0.026652 0.343998 0.296942 — 0.110140 0.405116 0.317055

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000124 0.000334 0.000225 0.001729 0.000146 0.000222 0.000278 0.001601 0.000136 0.000231 0.000235 0.001706
Bud Neck 0.013922 0.021982 0.001053 0.009671 0.010710 0.019773 0.001452 0.006673 0.012104 0.019938 0.001131 0.009138
Bud Site 0.009365 0.035574 0.008555 0.069807 0.008480 0.018162 0.006270 0.061724 0.008864 0.019464 0.008112 0.068370
Cell Periphery 0.000155 0.000096 0.000100 0.000371 0.000101 0.000101 0.000185 0.000175 0.000124 0.000101 0.000117 0.000336
Cytoplasm 0.586746* 0.474924* 0.713252* 0.616652* 0.549277* 0.466804* 0.506546* 0.391443* 0.565531* 0.467412* 0.673196* 0.576605*
Cytoplasmic Foci 0.201638* 0.204721* 0.028746 0.029704 0.217113* 0.215192* 0.060548 0.038797 0.210400* 0.214409* 0.034908 0.031321
Eisosomes 0.000136 0.000075 0.000024 0.000032 0.000100 0.000086 0.000088 0.000043 0.000116 0.000085 0.000037 0.000034
Endoplasmic Reticulum 0.001101 0.000520 0.001604 0.001139 0.002289 0.000887 0.001994 0.001282 0.001774 0.000860 0.001680 0.001164
Endosome 0.015944 0.043834 0.002477 0.010129 0.020035 0.042109 0.009333 0.025656 0.018261 0.042238 0.003806 0.012890
Golgi 0.003892 0.003522 0.000117 0.005574 0.005849 0.007461 0.000147 0.029243 0.005000 0.007166 0.000123 0.009783
Lipid Particles 0.003932 0.002050 0.000850 0.000659 0.004826 0.004080 0.002396 0.000681 0.004438 0.003929 0.001150 0.000663
Mitochondria 0.003189 0.003092 0.000109 0.008536 0.003010 0.006393 0.000069 0.011505 0.003088 0.006147 0.000101 0.009064
Mitotic Spindle 0.000747 0.005768 0.001139 0.028274 0.003223 0.005309 0.001758 0.109498* 0.002149 0.005343 0.001259 0.042718
None 0.006264 0.000520 0.003438 0.003828 0.001492 0.001160 0.001659 0.003343 0.003562 0.001112 0.003093 0.003742
Nuclear Periphery 0.000138 0.000164 0.000474 0.000453 0.000255 0.000276 0.000671 0.000580 0.000205 0.000267 0.000513 0.000476
Nuclear Periphery Foci 0.000434 0.000771 0.003950 0.001150 0.000402 0.000294 0.006329 0.001334 0.000416 0.000330 0.004411 0.001182
Nucleolus 0.000794 0.001197 0.000116 0.000222 0.000604 0.000792 0.000198 0.000150 0.000686 0.000823 0.000132 0.000209
Nucleus 0.060210 0.027156 0.143944* 0.066267 0.049397 0.037970 0.082575 0.040419 0.054088 0.037161 0.132052* 0.061671
Peroxisomes 0.001789 0.007175 0.000139 0.002757 0.002228 0.005026 0.000358 0.009267 0.002038 0.005187 0.000181 0.003914
Vacuole 0.086760 0.163768 0.089559 0.137528 0.118044 0.163223 0.316973* 0.252655* 0.104473 0.163264 0.133628 0.158000
Vacuole Periphery 0.002719 0.002755 0.000127 0.005516 0.002418 0.004679 0.000175 0.013932 0.002549 0.004536 0.000136 0.007013

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.65 -5.45 -2.91 -1.94 -2.07 -2.48 -3.43 -2.37 -1.90 -1.58 -3.86 -5.68 -3.20 -2.69 -2.30
Bud Neck -1.41 12.68 6.91 3.15 -4.83 -6.90 14.26 10.43 13.38 -2.11 -6.11 19.47 8.64 11.44 -5.07
Bud Site -4.57 0.51 -6.36 0.66 -6.10 -7.34 1.58 -1.75 0.31 -2.13 -8.46 0.55 -6.78 -2.88 -6.47
Cell Periphery 4.09 3.98 -0.76 -2.32 -1.92 -0.01 -3.53 2.49 1.83 4.43 2.31 0.48 -1.32 -2.03 -1.42
Cytoplasm 4.80 -6.40 7.71 -1.50 11.74 9.96 5.80 10.55 5.91 2.94 13.60 -4.78 9.61 -1.50 11.12
Cytoplasmic Foci -0.00 40.95 43.31 12.70 2.86 0.39 28.88 41.44 37.47 3.76 -0.68 56.25 62.43 43.95 4.43
Eisosomes 4.98 10.78 10.01 4.67 -3.70 2.74 2.87 12.68 9.30 5.83 5.26 14.84 15.70 11.76 0.33
Endoplasmic Reticulum 3.85 -4.36 0.15 -4.11 4.74 4.10 -0.02 3.50 -1.15 4.19 4.02 -1.12 3.40 -1.41 5.88
Endosome -4.88 15.49 11.50 7.16 -1.60 -11.19 10.60 7.49 14.93 0.75 -13.38 22.14 16.17 21.42 -0.88
Golgi 0.47 14.79 4.09 1.42 -3.21 -2.53 17.42 0.31 0.80 -1.54 -3.94 22.50 3.88 5.82 -3.12
Lipid Particles 3.30 12.63 13.71 2.02 1.75 1.63 7.40 13.34 8.74 4.11 1.33 16.03 18.76 9.20 3.91
Mitochondria 0.14 5.57 0.49 0.12 -3.00 -3.23 5.50 0.69 2.78 -1.57 -3.29 7.71 0.61 3.17 -3.34
Mitotic Spindle -1.91 -1.47 -6.02 -2.77 -5.32 -1.73 -0.29 -3.42 -3.15 -3.00 -3.08 -0.23 -6.39 -4.78 -5.96
None 8.00 3.71 3.75 -11.50 -0.83 1.18 0.56 -3.76 -4.10 -3.88 6.32 1.69 0.07 -7.82 -1.82
Nuclear Periphery -0.98 -16.34 -16.42 -13.27 -3.85 -0.30 -6.80 -7.42 -8.74 -1.22 -1.27 -10.02 -12.16 -12.17 -3.87
Nuclear Periphery Foci -1.88 -11.52 -10.35 -2.82 8.49 1.94 -8.38 -7.25 -7.76 6.62 1.63 -13.98 -12.55 -12.24 10.43
Nucleolus -0.59 12.39 9.90 1.48 -2.82 -2.03 8.65 9.98 7.17 0.45 -1.44 16.39 12.48 6.55 -2.57
Nucleus 6.04 -16.18 -11.48 -12.23 8.46 5.16 -5.72 -3.57 -5.29 2.10 8.44 -17.98 -13.89 -17.70 8.49
Peroxisomes -4.51 14.67 2.41 5.05 -2.72 -8.05 12.29 0.46 3.10 -1.47 -9.85 18.63 2.76 9.29 -3.07
Vacuole -5.73 -6.82 -24.02 -7.00 -15.52 -8.67 -13.60 -17.69 -15.13 -2.55 -12.86 -10.59 -27.36 -17.98 -13.27
Vacuole Periphery 0.12 8.09 1.79 1.38 -3.05 -6.09 13.70 0.01 1.61 -1.66 -5.61 14.42 1.51 4.80 -3.48
Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mag2

Mag2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mag2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available