ORF
Human Ortholog
Description Putative protein of unknown function

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.79 0.91 0.95 0.9 0.75 0.69 0.57 0.64 0.58 0.57 0.97 0.96 0.98 0.81 0.77 0.75 0.32 0.4 0.33 0.56 0.61 0.67
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0.1 0.09 0.07 0.1 0.05 0.1 0 0 0 0.07 0.07 0.09 0.06 0.07 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.09 0 0 0 0.05 0.12 0.28 0.21 0.39 0.31 0.08 0 0 0 0 0 0.35 0.27 0.36 0.2 0.11 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.07 0.12 0.1 0.1 0.11 0.1 0.09 0 0 0 0.09 0.16 0.17 0.16 0.15 0.13 0.16 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 1 1 0 0 0 0 0 0 2 2 2 0 2 4 0 0 1
Bud 0 0 0 0 0 2 6 6 4 7 3 1 0 0 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 2 0 1 1 4 0 3 0 1 0 2 0 0 1 0 1 1 2 0 1 1
Bud Site 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0
Cell Periphery 0 0 2 0 0 0 3 1 3 0 0 0 0 0 4 4 1 0 0 0 0 0 0
Cytoplasm 80 44 75 161 234 178 226 123 133 151 147 37 74 107 198 193 177 40 44 36 13 34 81
Endoplasmic Reticulum 1 5 1 2 0 4 1 2 6 1 2 0 1 0 9 9 14 9 3 2 0 1 1
Endosome 0 1 0 2 10 24 30 16 21 12 27 0 0 1 16 17 22 8 8 2 0 0 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 3 0 1 1 0 0 0
Mitochondria 7 5 0 2 3 13 38 60 44 100 80 3 0 0 6 4 7 44 30 40 4 6 13
Nucleus 0 0 0 0 0 0 6 4 0 4 4 1 0 0 2 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 2 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 1 4 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 4 3 8 11 5 2 13 0 0 0 4 3 0 1 1 0 0 0 0
Vac/Vac Membrane 0 3 1 5 17 29 31 22 22 25 24 0 0 0 22 39 40 20 17 14 3 7 14
Unique Cell Count 86 56 82 169 261 237 326 217 209 259 258 38 77 109 243 250 237 128 113 112 24 56 121
Labelled Cell Count 88 60 83 174 272 255 358 247 242 304 307 42 78 109 265 276 266 128 113 112 24 56 121


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 5.8 5.5 6.2 5.4 5.3 4.7 5.0 4.6 4.9 4.6 5.3 5.6 7.1 7.2 7.2 5.7 5.3
Std Deviation (1e-4) 0.4 1.0 1.0 1.6 1.9 2.5 1.5 1.4 1.4 1.1 1.1 0.5 0.9 1.7 1.3 1.3 1.6 1.8 1.1
Intensity Change (Log2) -0.07 0.11 -0.09 -0.13 -0.29 -0.2 -0.34 -0.25 -0.34 -0.12 -0.05 0.31 0.32 0.31 -0.02 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.4 3.5 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0907 0.0007 0.0017 0.011 0.0008 0.0078 0.0272 0.0002 0.0001 0.0001 0.0001 0.0009
Bud 0.0084 0.0044 0.0021 0.0005 0.0011 0 0.0009 0.0004 0.0008 0.0001 0 0.0001
Bud Neck 0.0016 0.0002 0.0009 0.0003 0.0121 0.0003 0.0061 0 0.0001 0 0.0085 0.0005
Bud Periphery 0.0125 0.0012 0.0049 0.0002 0.0014 0 0.0014 0.0001 0.0004 0.0002 0 0.0002
Bud Site 0.0057 0.0032 0.0102 0.0008 0.01 0.0001 0.0191 0.0005 0.0041 0 0.0001 0.0001
Cell Periphery 0.0037 0.0002 0.0001 0 0.0005 0 0.0006 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0542 0.0356 0.0319 0.0268 0.0234 0.0562 0.065 0.0823 0.0756 0.0847 0.1702 0.1223
Cytoplasmic Foci 0.0542 0.0047 0.0088 0.0159 0.0299 0.0092 0.0181 0.0055 0.0061 0.0016 0.0043 0.0067
Eisosomes 0.0012 0.0002 0.0001 0 0 0 0.0014 0.0001 0 0 0 0.0001
Endoplasmic Reticulum 0.0052 0.0008 0.0007 0.0003 0.0003 0.0003 0.0038 0.0003 0.0004 0.0029 0.001 0.0006
Endosome 0.0587 0.0037 0.0045 0.0113 0.0491 0.0036 0.0185 0.0009 0.0012 0.0012 0.0008 0.0065
Golgi 0.0231 0.0019 0.0008 0.0244 0.0188 0.0043 0.0052 0.0001 0 0 0 0.0003
Lipid Particles 0.0342 0.0012 0.0009 0.0001 0.0188 0.0004 0.0069 0.0002 0.0001 0 0 0.0005
Mitochondria 0.006 0.0008 0.0225 0.0052 0.002 0.0004 0.0035 0.0002 0.0002 0.0002 0.0001 0.0008
None 0.5387 0.9375 0.8994 0.9007 0.8097 0.9153 0.7809 0.9054 0.9075 0.9055 0.8128 0.855
Nuclear Periphery 0.0028 0.0002 0.0025 0.0001 0.0003 0.0001 0.0079 0.0003 0.0003 0.0003 0.0003 0.0011
Nucleolus 0.0003 0.0004 0.0007 0 0.0018 0 0.0037 0.0002 0.0001 0 0 0.0003
Nucleus 0.0026 0.0004 0.002 0.0001 0.0008 0.0001 0.0051 0.0011 0.0006 0.0003 0.0008 0.001
Peroxisomes 0.0864 0.0003 0.0013 0.0019 0.0057 0.0013 0.0154 0.0005 0.0001 0 0.0001 0.0002
Punctate Nuclear 0.0018 0.0004 0.0021 0.0001 0.0011 0.0002 0.0052 0.0012 0.001 0.0002 0.0003 0.0015
Vacuole 0.0069 0.0019 0.0014 0.0001 0.0113 0.0001 0.0029 0.0004 0.0008 0.0022 0.0003 0.0012
Vacuole Periphery 0.0012 0.0002 0.0005 0.0001 0.001 0 0.001 0.0001 0.0001 0.0003 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2963 7.4164 4.4026 9.615 7.171 14.925 5.1241 8.2665 8.542 12.3794
Translational Efficiency 1.0544 0.4906 0.6262 0.4791 0.9386 0.542 0.9403 0.4091 0.5068 0.5629

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Putative protein of unknown function
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YML020W

YML020W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml020w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available