ORF
Human Ortholog
Description Putative protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.79 0.91 0.95 0.9 0.75 0.69 – 0.57 0.64 0.58 0.57 0.97 0.96 0.98 0.81 0.77 0.75 0.32 0.4 0.33 0.56 0.61 0.67
Endoplasmic Reticulum 0 0.09 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0.1 0.09 – 0.07 0.1 0.05 0.1 0 0 0 0.07 0.07 0.09 0.06 0.07 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.09 0 0 0 0.05 0.12 – 0.28 0.21 0.39 0.31 0.08 0 0 0 0 0 0.35 0.27 0.36 0.2 0.11 0.11
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.07 0.12 0.1 – 0.1 0.11 0.1 0.09 0 0 0 0.09 0.16 0.17 0.16 0.15 0.13 0.16 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 1 – 1 0 0 0 0 0 0 2 2 2 0 2 4 0 0 1
Bud 0 0 0 0 0 2 6 – 6 4 7 3 1 0 0 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 2 0 1 1 4 – 0 3 0 1 0 2 0 0 1 0 1 1 2 0 1 1
Bud Site 0 0 0 0 0 0 0 – 1 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 0 3 – 1 3 0 0 0 0 0 4 4 1 0 0 0 0 0 0
Cytoplasm 80 44 75 161 234 178 226 – 123 133 151 147 37 74 107 198 193 177 40 44 36 13 34 81
Endoplasmic Reticulum 1 5 1 2 0 4 1 – 2 6 1 2 0 1 0 9 9 14 9 3 2 0 1 1
Endosome 0 1 0 2 10 24 30 – 16 21 12 27 0 0 1 16 17 22 8 8 2 0 0 1
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 1 0 3 0 1 1 0 0 0
Mitochondria 7 5 0 2 3 13 38 – 60 44 100 80 3 0 0 6 4 7 44 30 40 4 6 13
Nucleus 0 0 0 0 0 0 6 – 4 0 4 4 1 0 0 2 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 – 0 0 1 2 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 1 4 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 4 3 8 – 11 5 2 13 0 0 0 4 3 0 1 1 0 0 0 0
Vac/Vac Membrane 0 3 1 5 17 29 31 – 22 22 25 24 0 0 0 22 39 40 20 17 14 3 7 14
Unique Cell Count 86 56 82 169 261 237 326 217 209 259 258 38 77 109 243 250 237 128 113 112 24 56 121
Labelled Cell Count 88 60 83 174 272 255 358 247 242 304 307 42 78 109 265 276 266 128 113 112 24 56 121


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 5.8 5.5 6.2 5.4 5.3 – 4.7 5.0 4.6 4.9 4.6 5.3 5.6 7.1 7.2 7.2 – 5.7 5.3
Std Deviation (1e-4) 0.4 1.0 1.0 1.6 1.9 2.5 1.5 – 1.4 1.4 1.1 1.1 0.5 0.9 1.7 1.3 1.3 1.6 – 1.8 1.1
Intensity Change (Log2) – – – -0.07 0.11 -0.09 -0.13 – -0.29 -0.2 -0.34 -0.25 -0.34 -0.12 -0.05 0.31 0.32 0.31 – -0.02 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.4 3.5 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0907 0.0007 0.0017 0.011 0.0008 0.0078 0.0272 0.0002 0.0001 0.0001 0.0001 0.0009
Bud – – – – – – 0.0084 0.0044 0.0021 0.0005 0.0011 0 0.0009 0.0004 0.0008 0.0001 0 0.0001
Bud Neck – – – – – – 0.0016 0.0002 0.0009 0.0003 0.0121 0.0003 0.0061 0 0.0001 0 0.0085 0.0005
Bud Periphery – – – – – – 0.0125 0.0012 0.0049 0.0002 0.0014 0 0.0014 0.0001 0.0004 0.0002 0 0.0002
Bud Site – – – – – – 0.0057 0.0032 0.0102 0.0008 0.01 0.0001 0.0191 0.0005 0.0041 0 0.0001 0.0001
Cell Periphery – – – – – – 0.0037 0.0002 0.0001 0 0.0005 0 0.0006 0.0001 0.0001 0.0001 0 0
Cytoplasm – – – – – – 0.0542 0.0356 0.0319 0.0268 0.0234 0.0562 0.065 0.0823 0.0756 0.0847 0.1702 0.1223
Cytoplasmic Foci – – – – – – 0.0542 0.0047 0.0088 0.0159 0.0299 0.0092 0.0181 0.0055 0.0061 0.0016 0.0043 0.0067
Eisosomes – – – – – – 0.0012 0.0002 0.0001 0 0 0 0.0014 0.0001 0 0 0 0.0001
Endoplasmic Reticulum – – – – – – 0.0052 0.0008 0.0007 0.0003 0.0003 0.0003 0.0038 0.0003 0.0004 0.0029 0.001 0.0006
Endosome – – – – – – 0.0587 0.0037 0.0045 0.0113 0.0491 0.0036 0.0185 0.0009 0.0012 0.0012 0.0008 0.0065
Golgi – – – – – – 0.0231 0.0019 0.0008 0.0244 0.0188 0.0043 0.0052 0.0001 0 0 0 0.0003
Lipid Particles – – – – – – 0.0342 0.0012 0.0009 0.0001 0.0188 0.0004 0.0069 0.0002 0.0001 0 0 0.0005
Mitochondria – – – – – – 0.006 0.0008 0.0225 0.0052 0.002 0.0004 0.0035 0.0002 0.0002 0.0002 0.0001 0.0008
None – – – – – – 0.5387 0.9375 0.8994 0.9007 0.8097 0.9153 0.7809 0.9054 0.9075 0.9055 0.8128 0.855
Nuclear Periphery – – – – – – 0.0028 0.0002 0.0025 0.0001 0.0003 0.0001 0.0079 0.0003 0.0003 0.0003 0.0003 0.0011
Nucleolus – – – – – – 0.0003 0.0004 0.0007 0 0.0018 0 0.0037 0.0002 0.0001 0 0 0.0003
Nucleus – – – – – – 0.0026 0.0004 0.002 0.0001 0.0008 0.0001 0.0051 0.0011 0.0006 0.0003 0.0008 0.001
Peroxisomes – – – – – – 0.0864 0.0003 0.0013 0.0019 0.0057 0.0013 0.0154 0.0005 0.0001 0 0.0001 0.0002
Punctate Nuclear – – – – – – 0.0018 0.0004 0.0021 0.0001 0.0011 0.0002 0.0052 0.0012 0.001 0.0002 0.0003 0.0015
Vacuole – – – – – – 0.0069 0.0019 0.0014 0.0001 0.0113 0.0001 0.0029 0.0004 0.0008 0.0022 0.0003 0.0012
Vacuole Periphery – – – – – – 0.0012 0.0002 0.0005 0.0001 0.001 0 0.001 0.0001 0.0001 0.0003 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2963 7.4164 4.4026 9.615 7.171 14.925 5.1241 8.2665 8.542 12.3794
Translational Efficiency 1.0544 0.4906 0.6262 0.4791 0.9386 0.542 0.9403 0.4091 0.5068 0.5629

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1938 1348 449 1301 1407 1387 2598 192 3345 2735 3047 1493

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 667.22 733.94 975.30 874.59 698.98 702.99 952.26 867.41 680.58 718.24 955.66 873.67
Standard Deviation 77.50 97.38 127.13 126.96 76.35 103.11 111.10 114.14 78.60 101.52 113.90 125.41
Intensity Change Log 2 — 0.137500 0.547684 0.390444 — 0.008253 0.446104 0.311463 — 0.072821 0.496607 0.350576

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000180 0.000692 0.000916 0.003491 0.000281 0.000882 0.000117 0.000517 0.000222 0.000789 0.000235 0.003108
Bud Neck 0.046793 0.051585 0.006218 0.011487 0.043395 0.054185 0.004465 0.008510 0.045364 0.052903 0.004723 0.011105
Bud Site 0.014371 0.040317 0.007248 0.050368 0.014983 0.036800 0.006598 0.033190 0.014628 0.038533 0.006694 0.048159
Cell Periphery 0.000256 0.000179 0.000183 0.000201 0.000163 0.000237 0.000055 0.000177 0.000217 0.000208 0.000073 0.000198
Cytoplasm 0.371292* 0.269459* 0.295593* 0.440262* 0.332842* 0.242451* 0.632796* 0.330550* 0.355119* 0.255762* 0.583107* 0.426153*
Cytoplasmic Foci 0.263084* 0.344228* 0.010656 0.070234 0.332101* 0.313533* 0.013048 0.079067 0.292114* 0.328662* 0.012695 0.071370
Eisosomes 0.000203 0.000206 0.000057 0.000083 0.000261 0.000261 0.000015 0.000090 0.000227 0.000234 0.000022 0.000084
Endoplasmic Reticulum 0.002764 0.000949 0.011867 0.003070 0.001236 0.001255 0.001623 0.003429 0.002121 0.001105 0.003132 0.003116
Endosome 0.042496 0.058927 0.004997 0.011517 0.053955 0.076226 0.001749 0.020233 0.047316 0.067700 0.002228 0.012638
Golgi 0.005578 0.025500 0.000190 0.010739 0.012625 0.027199 0.000355 0.004756 0.008542 0.026361 0.000331 0.009969
Lipid Particles 0.016876 0.010427 0.002573 0.002689 0.014381 0.012545 0.000693 0.004008 0.015827 0.011501 0.000970 0.002859
Mitochondria 0.009024 0.029156 0.000765 0.002804 0.014050 0.044176 0.000524 0.000521 0.011138 0.036773 0.000559 0.002511
Mitotic Spindle 0.002996 0.008423 0.015849 0.034239 0.001914 0.016739 0.006546 0.021306 0.002541 0.012640 0.007917 0.032576
None 0.004343 0.002091 0.005483 0.002279 0.001894 0.003848 0.004028 0.004378 0.003313 0.002982 0.004242 0.002549
Nuclear Periphery 0.000683 0.000389 0.003453 0.001354 0.000456 0.000348 0.001884 0.001158 0.000588 0.000368 0.002115 0.001328
Nuclear Periphery Foci 0.001041 0.000859 0.007620 0.002189 0.000431 0.000606 0.001135 0.002599 0.000784 0.000731 0.002090 0.002241
Nucleolus 0.003028 0.001592 0.000838 0.000557 0.001372 0.002514 0.000358 0.002100 0.002331 0.002059 0.000428 0.000755
Nucleus 0.101523 0.038186 0.485024* 0.142028* 0.055862 0.033418 0.242642* 0.175567* 0.082317 0.035768 0.278359* 0.146341*
Peroxisomes 0.007649 0.038676 0.001193 0.007457 0.014502 0.034241 0.000218 0.003934 0.010532 0.036427 0.000362 0.007004
Vacuole 0.102479 0.072907 0.138924 0.198299 0.099284 0.090661 0.081002 0.300298* 0.101135 0.081910 0.089537 0.211416*
Vacuole Periphery 0.003341 0.005252 0.000353 0.004653 0.004011 0.007875 0.000151 0.003613 0.003623 0.006583 0.000181 0.004519

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.95 -9.52 -3.66 -2.70 -1.82 -2.82 4.04 -1.01 2.07 -2.56 -5.08 -1.71 -3.62 -2.36 -3.52
Bud Neck -1.12 15.24 14.47 13.27 -1.53 -2.95 16.62 12.94 14.55 -0.92 -2.72 23.24 19.28 19.74 -5.46
Bud Site -9.60 4.26 -6.04 1.81 -8.35 -8.35 7.57 -0.70 2.31 -2.15 -12.67 9.14 -6.03 2.18 -9.61
Cell Periphery 1.09 0.70 1.32 1.03 2.49 -4.01 13.79 0.56 2.62 -3.07 0.21 3.49 1.37 3.31 -7.65
Cytoplasm 10.82 11.64 -1.00 -10.36 -11.43 9.19 -24.46 1.75 -2.37 11.81 14.65 -21.25 -1.68 -12.65 14.45
Cytoplasmic Foci -10.12 50.54 38.35 42.84 -14.60 2.21 53.22 30.70 27.91 -6.67 -6.13 72.42 53.05 54.39 -16.29
Eisosomes -0.21 14.91 14.99 12.82 -0.68 0.04 17.95 8.27 6.35 -6.04 -0.43 27.34 18.52 12.84 -14.48
Endoplasmic Reticulum 4.93 -11.93 -1.15 -8.97 11.92 -0.06 -3.76 -3.18 -2.73 -2.02 3.68 -5.75 -3.78 -7.71 1.90
Endosome -4.79 18.57 17.17 18.54 -1.75 -5.76 21.61 12.26 16.78 -4.55 -7.92 29.47 23.79 28.20 -7.77
Golgi -11.56 12.40 0.43 9.21 -3.22 -6.98 13.48 5.37 9.85 -1.23 -13.29 17.48 2.88 12.01 -3.37
Lipid Particles 4.96 15.26 15.75 8.17 1.69 1.08 12.61 8.88 6.36 -3.87 4.07 21.42 18.48 10.62 -6.73
Mitochondria -8.11 10.83 8.15 11.45 -1.63 -8.26 8.30 8.40 13.47 1.96 -11.72 12.99 10.45 17.06 -2.13
Mitotic Spindle -3.24 -2.94 -6.52 -4.60 -1.56 -6.43 -4.41 -2.63 -0.58 -1.89 -7.01 -5.03 -7.23 -3.50 -4.97
None 3.44 -0.13 3.70 -0.10 4.29 -2.00 -6.42 -1.14 0.16 0.43 0.54 -2.43 2.20 1.11 5.38
Nuclear Periphery 1.32 -11.76 -4.67 -7.41 8.65 0.58 -11.15 -4.51 -6.91 6.35 1.49 -14.30 -6.27 -9.13 6.38
Nuclear Periphery Foci 0.47 -6.01 -2.12 -2.58 5.17 -1.31 -6.31 -3.92 -3.54 -2.41 0.26 -5.60 -3.72 -3.99 0.53
Nucleolus 3.76 5.00 7.77 5.38 2.08 -2.80 5.41 0.23 1.72 -1.10 0.54 9.22 7.49 5.86 -1.29
Nucleus 17.40 -27.03 -10.07 -21.27 21.32 6.76 -44.17 -6.77 -8.26 7.64 18.30 -46.22 -14.63 -23.64 21.04
Peroxisomes -13.67 12.96 3.53 14.34 -3.45 -8.86 13.41 9.27 15.31 -2.82 -16.52 19.88 6.94 19.31 -4.47
Vacuole 6.67 -8.96 -23.98 -27.52 -6.81 1.41 -6.89 -12.67 -12.94 -11.20 5.39 -10.21 -27.46 -29.60 -20.48
Vacuole Periphery -3.90 11.92 1.85 4.35 -3.41 -5.09 9.50 2.08 5.19 -1.73 -6.62 15.39 2.67 7.00 -4.06
ORF
Human Ortholog
Description Putative protein of unknown function
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YML020W

YML020W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml020w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available