Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.98 0.95 0.96 0.81 0.8 0.86 0.76 0.76 0.99 0.99 0.99 0.96 0.99 0.94 0.95 0.9 0.91 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 8 4 2 2 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 0 0 0 2 0 0 2 1 0 0 3 0 1 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 0 2 1 4 5 4 2 5 12 0 1 1 1 0 0 0 0 0 0 1
Cytoplasm 482 163 71 135 140 216 198 140 190 139 155 338 354 383 110 113 455 163 79 172 215 250
Endoplasmic Reticulum 0 0 0 1 1 2 20 13 8 10 9 1 4 2 5 1 1 0 0 1 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 3 0 2 0 0 1 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 1 0 3 5 5 7 23 27 0 0 0 0 1 0 0 1 0 0 2
Nucleus 0 0 0 0 0 0 1 3 7 5 5 2 0 1 1 0 9 3 1 5 3 2
Nuclear Periphery 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 1 1 7 2 19 10 8 5 5 0 1 0 4 2 5 2 1 2 4 3
Unique Cell Count 487 163 73 138 148 224 244 174 221 184 203 341 359 385 114 114 484 172 88 189 229 274
Labelled Cell Count 489 163 73 140 149 227 254 184 228 194 215 343 362 388 121 117 484 172 88 189 229 274


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.7 42.5 25.1 35.2 36.5 30.0 24.1 21.3 20.8 18.9 19.4 42.6 43.8 41.9 64.4 64.5 33.7 38.3 38.0
Std Deviation (1e-4) 8.4 7.8 9.0 9.8 10.4 7.4 5.8 5.2 5.4 4.9 5.1 9.7 10.2 9.6 26.7 24.7 9.3 9.5 9.7
Intensity Change (Log2) 0.49 0.54 0.26 -0.05 -0.24 -0.27 -0.41 -0.37 0.77 0.81 0.74 1.36 1.36 0.43 0.61 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.9 -0.3 -3.4 -3.4 -2.6 -4.1 -4.0 1.3 0.8 1.9 -0.3 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.2 3.3 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.6944 49.3251 43.2261 40.7137 37.3802 44.9912 35.3943 45.5439 41.6493 32.8615 28.4811 41.5547 37.4792 51.1918 47.5574 45.6657 48.3209 51.418
Actin 0.017 0.0005 0.0007 0.002 0.0001 0.0026 0.0148 0.0001 0.0106 0.0431 0.0232 0.0002 0.0411 0.0003 0.0002 0.0001 0.0003 0
Bud 0.0008 0.0001 0.0001 0.0013 0 0.0001 0.0006 0 0.0039 0.0029 0.0104 0.0001 0.0009 0.0001 0.0004 0.0001 0.0002 0
Bud Neck 0.0004 0.0001 0.0002 0.0002 0.0002 0.0005 0.0004 0.0001 0.0007 0.0008 0.0014 0.0006 0.0037 0.0001 0.0002 0.0002 0.0003 0.0003
Bud Periphery 0.0005 0.0001 0.0001 0.0012 0 0 0.0007 0 0.0031 0.0043 0.0244 0 0.001 0 0.0002 0 0.0004 0
Bud Site 0.0032 0.0002 0.0005 0.0012 0 0.0001 0.0032 0 0.0097 0.003 0.0016 0.0001 0.0065 0.0001 0.0004 0.0001 0.0001 0
Cell Periphery 0.0002 0 0.0001 0.001 0 0 0.0003 0 0.0008 0.0004 0.0006 0 0.0004 0 0 0.0001 0.0004 0
Cytoplasm 0.8572 0.9814 0.9367 0.9101 0.9253 0.9447 0.7863 0.9703 0.8981 0.8152 0.6335 0.9638 0.7602 0.9853 0.95 0.9195 0.9212 0.9702
Cytoplasmic Foci 0.0171 0.0008 0.0148 0.0121 0.0137 0.0079 0.0337 0.0001 0.0107 0.0065 0.045 0.0037 0.0345 0.0001 0.0102 0.0201 0.002 0.0002
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0.0003 0 0.0002 0.0001 0.0003 0 0.0006 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0039 0.0001 0.0009 0.0023 0.0002 0.0009 0.0046 0.0001 0.0011 0.0089 0.0056 0.0012 0.0047 0.0001 0.0003 0.0001 0.0026 0.0006
Endosome 0.0132 0 0.005 0.0065 0.0258 0.0018 0.0254 0 0.007 0.05 0.0493 0.0018 0.0274 0 0.0039 0.0007 0.008 0.0001
Golgi 0.0045 0 0.0022 0.0006 0.0029 0.0022 0.0133 0 0.0062 0.0113 0.0248 0.0001 0.0098 0 0.0009 0.0002 0.0006 0
Lipid Particles 0.0066 0.0001 0.0026 0.0061 0.013 0.0022 0.0189 0 0.0011 0.0022 0.0099 0.0002 0.0194 0 0.0063 0.0143 0.0026 0
Mitochondria 0.0074 0.0053 0.0022 0.0082 0.0002 0.0003 0.0382 0.0158 0.0172 0.0073 0.0285 0.0001 0.0186 0.0001 0.005 0.0021 0.0129 0
None 0.0125 0.0009 0.004 0.0067 0.0002 0.0011 0.0054 0.0001 0.002 0.0011 0.0241 0.0006 0.0108 0.0002 0.0005 0.0005 0.0057 0.0001
Nuclear Periphery 0.0085 0.0008 0.0021 0.0025 0.0019 0.0052 0.007 0.0006 0.0046 0.0067 0.0364 0.003 0.0094 0.0013 0.0016 0.0027 0.0039 0.0024
Nucleolus 0.0013 0.0001 0.0003 0.0016 0 0 0.001 0 0.0017 0.0002 0.0019 0.0001 0.0014 0 0.0007 0.0121 0.0005 0
Nucleus 0.0315 0.0082 0.0159 0.0123 0.0128 0.023 0.0302 0.0126 0.0141 0.0088 0.0236 0.022 0.022 0.0119 0.0147 0.0228 0.0192 0.0257
Peroxisomes 0.0034 0.0009 0.0008 0.0012 0.0029 0.0064 0.0033 0 0.0019 0.0006 0.0043 0 0.0155 0 0.0029 0.0011 0.0002 0
Punctate Nuclear 0.0044 0.0001 0.0012 0.0006 0.0003 0.0006 0.0096 0.0001 0.0019 0.0009 0.0034 0.001 0.0074 0.0002 0.0003 0.0014 0.0002 0.0001
Vacuole 0.0041 0.0001 0.006 0.017 0.0004 0.0003 0.0018 0 0.002 0.0142 0.0328 0.0011 0.003 0.0001 0.001 0.001 0.0116 0.0001
Vacuole Periphery 0.0023 0 0.0035 0.0052 0.0002 0.0001 0.001 0 0.0015 0.0115 0.0147 0.0001 0.0017 0 0.0004 0.0011 0.0067 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 242.4679 280.6251 212.8979 250.4587 201.9866 259.894 388.0247 384.4395 361.4375 265.6626
Translational Efficiency 1.8449 2.1368 2.3273 1.7041 2.0794 2.5833 2.1016 2.7298 2.3419 2.3972

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1237 1462 1453 1667 1836 913 2124 1470 3073 2375 3577 3137

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1466.67 2706.93 3250.53 4306.74 2306.66 2706.23 3380.73 4105.66 1968.53 2706.66 3327.84 4212.51
Standard Deviation 342.87 552.05 582.29 930.44 447.87 516.52 639.39 900.19 580.40 538.67 620.14 921.87
Intensity Change Log 2 0.884113 1.148131 1.554052 0.230479 0.551529 0.831809 0.520633 0.813445 1.156679

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000204 0.000156 0.000089 0.001200 0.000111 0.000301 0.000107 0.000657 0.000148 0.000212 0.000100 0.000946
Bud Neck 0.000285 0.000491 0.000601 0.008076 0.000300 0.001134 0.002271 0.002307 0.000294 0.000738 0.001593 0.005372
Bud Site 0.001382 0.000848 0.000446 0.018495 0.000836 0.002626 0.001725 0.010971 0.001056 0.001531 0.001206 0.014969
Cell Periphery 0.000160 0.000155 0.000225 0.000627 0.000110 0.000253 0.000103 0.000570 0.000130 0.000193 0.000153 0.000600
Cytoplasm 0.760081* 0.639759* 0.450209* 0.605105* 0.719863* 0.693850* 0.538548* 0.714848* 0.736052* 0.660553* 0.502664* 0.656530*
Cytoplasmic Foci 0.003458 0.004024 0.003802 0.007751 0.002945 0.010452 0.001764 0.008160 0.003152 0.006495 0.002592 0.007942
Eisosomes 0.000002 0.000001 0.000001 0.000012 0.000001 0.000002 0.000002 0.000003 0.000001 0.000002 0.000001 0.000008
Endoplasmic Reticulum 0.000285 0.000264 0.000031 0.000391 0.000191 0.000457 0.000115 0.000077 0.000229 0.000339 0.000081 0.000244
Endosome 0.000110 0.000341 0.000022 0.004125 0.000068 0.000466 0.000432 0.004240 0.000085 0.000389 0.000265 0.004179
Golgi 0.000223 0.000836 0.000132 0.006901 0.000796 0.000719 0.000172 0.004181 0.000565 0.000791 0.000156 0.005626
Lipid Particles 0.000107 0.000244 0.000159 0.001713 0.000121 0.000573 0.000247 0.000907 0.000115 0.000371 0.000211 0.001335
Mitochondria 0.000777 0.000164 0.000219 0.008852 0.000184 0.000223 0.000584 0.002447 0.000423 0.000187 0.000436 0.005851
Mitotic Spindle 0.001206 0.003303 0.000356 0.045908 0.000660 0.008323 0.001956 0.007269 0.000880 0.005232 0.001306 0.027802
None 0.019459 0.019750 0.013355 0.010366 0.013394 0.011675 0.015316 0.016067 0.015835 0.016646 0.014519 0.013038
Nuclear Periphery 0.000055 0.000106 0.000172 0.001050 0.000100 0.000173 0.000273 0.000213 0.000082 0.000132 0.000232 0.000658
Nuclear Periphery Foci 0.002540 0.002031 0.000310 0.002428 0.001212 0.002598 0.000840 0.001653 0.001747 0.002249 0.000625 0.002065
Nucleolus 0.000110 0.000290 0.000056 0.001698 0.000064 0.000276 0.000206 0.000185 0.000083 0.000285 0.000145 0.000989
Nucleus 0.205254* 0.322986* 0.527399* 0.241968* 0.254874* 0.256290* 0.428637* 0.210927* 0.234900* 0.297347* 0.468755* 0.227422*
Peroxisomes 0.000015 0.000032 0.000004 0.000313 0.000007 0.000032 0.000127 0.000278 0.000010 0.000032 0.000077 0.000297
Vacuole 0.004138 0.003425 0.002382 0.025269 0.002680 0.008004 0.006170 0.013074 0.003267 0.005186 0.004632 0.019554
Vacuole Periphery 0.000149 0.000791 0.000030 0.007752 0.001484 0.001572 0.000404 0.000968 0.000947 0.001091 0.000252 0.004573

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.12 0.79 -1.83 -1.85 -2.17 -4.79 -1.69 -1.84 0.85 -1.45 -4.01 -0.81 -2.79 -0.73 -2.58
Bud Neck -5.75 -8.35 -8.58 -7.55 -5.61 -6.77 -6.31 -6.15 -0.88 1.54 -8.86 -8.32 -10.41 -7.21 -2.72
Bud Site 1.40 2.09 -5.26 -5.83 -6.04 -4.79 -3.09 -4.63 -2.34 -3.35 -2.98 -1.89 -7.13 -6.05 -6.35
Cell Periphery -1.59 -7.30 -4.61 -4.13 -0.50 -7.04 -5.92 -7.11 -2.51 -4.85 -6.35 -9.31 -7.74 -5.11 -3.49
Cytoplasm 18.05 45.93 19.30 1.36 -25.83 5.49 37.35 0.33 -4.90 -33.58 16.97 57.41 14.43 -3.11 -39.08
Cytoplasmic Foci -3.35 -6.56 -7.52 -3.67 -0.05 -9.47 -3.42 -11.19 3.04 -8.24 -9.64 -7.08 -13.37 -0.42 -6.15
Eisosomes 0.68 -0.64 -4.77 -5.40 -4.92 -7.45 -9.79 -9.88 -1.40 -0.34 -3.11 -7.02 -8.95 -5.55 -4.07
Endoplasmic Reticulum 0.46 6.71 5.32 1.10 -1.78 -1.19 1.27 2.33 2.58 3.71 -0.48 3.47 3.96 2.29 1.44
Endosome -1.07 3.80 -3.22 -1.66 -3.63 -2.34 -1.80 -2.05 -1.28 -1.79 -2.15 -1.04 -3.20 -1.95 -3.00
Golgi -2.36 -0.06 -2.14 -0.81 -2.14 0.41 2.69 -0.65 -0.95 -2.23 -0.83 2.67 -1.70 -1.19 -3.11
Lipid Particles -5.76 -7.01 -11.91 -10.03 -8.68 -7.08 -8.13 -9.27 -1.97 -5.51 -8.60 -10.72 -14.90 -9.54 -10.27
Mitochondria 0.89 0.71 -0.81 -2.71 -2.46 -0.15 -2.24 -0.97 -0.95 -0.49 0.88 0.23 -1.49 -2.66 -2.10
Mitotic Spindle -1.07 1.43 -4.76 -3.93 -5.71 -2.87 -4.20 -2.41 1.50 -0.00 -3.18 -2.23 -5.56 -2.06 -4.65
None 0.62 4.02 7.18 11.04 10.72 3.28 -0.64 3.81 0.99 4.90 0.54 3.06 8.57 9.22 10.11
Nuclear Periphery -3.70 -12.21 -9.31 -8.15 -4.43 -3.57 -11.59 -3.27 0.37 9.28 -4.15 -15.29 -9.29 -7.35 1.09
Nuclear Periphery Foci 0.75 5.72 3.03 1.02 -8.11 -3.98 2.70 -0.55 3.73 -3.37 -1.64 6.00 1.74 2.60 -6.44
Nucleolus -1.03 2.00 -2.36 -0.92 -2.57 -2.58 -4.28 -2.22 1.89 2.47 -1.74 -3.31 -2.66 -0.34 -1.92
Nucleus -17.91 -47.06 -5.64 13.05 43.12 -0.25 -31.57 10.66 8.64 41.13 -13.33 -53.12 3.55 16.43 56.53
Peroxisomes -0.74 1.72 -2.98 -1.54 -3.34 -4.24 -1.44 -2.56 -1.47 -0.91 -1.87 -1.29 -3.90 -2.07 -1.94
Vacuole -1.72 -3.05 -24.68 -23.88 -23.59 -9.08 -16.59 -19.53 -9.57 -5.79 -8.58 -16.32 -30.98 -25.41 -20.19
Vacuole Periphery -1.47 1.46 -2.60 -1.10 -2.87 0.49 2.38 2.13 1.26 -0.92 0.23 2.50 0.29 0.02 -2.94
Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Mix17

Mix17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mix17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available