Standard name
Human Ortholog
Description Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.13 0.06 0.17 0.23 0.22 – 0.21 0.18 0.21 0.16 0.1 0.14 0.23 0.12 0.09 0.08 0 0 0.06 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0.08 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 0 0.1 0.05 0.48 0.17 – 0.45 0.49 0.5 0.5 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.91 0.74 0.79 0.82 0.68 0.44 0.5 – 0.41 0.44 0.43 0.46 0.85 0.79 0.63 0.79 0.76 0.76 0.85 0.72 0.72 0.75 0.59 0.44
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.18 0.05 0 0.1 0.06 0.12 – 0.06 0.06 0.05 0.06 0.06 0.08 0.16 0.11 0.15 0.19 0.08 0.15 0.15 0.14 0.21 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 1 2 0 – 12 8 11 8 0 1 0 0 0 0 6 3 2 2 10 11
Bud Neck 0 0 2 0 1 2 1 – 5 1 2 3 0 0 0 0 0 0 0 0 0 0 0 4
Bud Site 0 0 0 0 0 6 1 – 4 6 5 9 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 11 30 20 62 73 106 – 89 85 71 86 20 52 82 16 7 12 6 2 16 4 11 15
Endoplasmic Reticulum 0 2 0 0 0 0 0 – 0 0 0 0 1 1 1 8 2 3 0 0 0 0 0 3
Endosome 0 0 4 8 20 3 36 – 14 11 4 3 0 3 5 0 1 2 0 2 0 1 2 14
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 0 0 2 1 1
Mitochondria 7 27 7 32 16 154 82 – 187 227 168 277 3 8 1 3 4 7 2 3 1 2 5 7
Nucleus 345 101 179 270 241 142 238 – 173 207 144 256 171 291 223 105 62 121 345 111 197 139 192 143
Nuclear Periphery 0 0 0 0 1 0 0 – 2 2 1 3 0 0 0 0 1 1 0 0 0 0 1 2
Nucleolus 0 0 0 2 0 0 1 – 1 1 4 5 0 0 1 0 0 0 0 0 0 2 13 12
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 6 6 14 – 6 11 4 9 0 2 0 0 0 0 6 3 9 2 12 14
Vac/Vac Membrane 24 24 12 13 34 19 55 – 24 29 18 31 12 28 58 15 12 30 32 23 41 25 67 86
Unique Cell Count 378 136 226 329 355 321 477 419 467 338 552 202 370 353 133 82 160 406 154 275 186 327 323
Labelled Cell Count 392 166 235 348 382 407 534 517 588 432 690 207 388 372 147 89 177 406 154 275 186 327 323


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.5 5.6 5.5 6.0 4.3 5.5 – 4.3 4.5 4.4 4.4 6.0 5.2 6.2 6.6 7.4 7.5 5.2 5.4 5.4
Std Deviation (1e-4) 0.6 0.6 1.5 1.5 1.9 1.9 4.4 – 1.5 1.6 1.5 3.3 1.3 1.2 1.7 1.2 1.8 1.5 1.6 1.5 1.2
Intensity Change (Log2) – – – -0.01 0.11 -0.39 -0.01 – -0.39 -0.31 -0.34 -0.33 0.12 -0.09 0.14 0.24 0.42 0.42 -0.1 -0.05 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.1 2.7 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4116 -0.0841 -0.462 -0.4768 -0.8874 -0.4613 2.0405 1.7479 1.863 1.4239 1.2983 1.7376 3.6535 3.8566 3.5825 3.0327 3.1995 3.4465
Actin 0.0109 0 0.0002 0.0008 0 0.0004 0.0289 0 0.0119 0.0321 0.0045 0.0063 0.008 0.0001 0.0134 0 0.0182 0.0037
Bud 0.0006 0 0.0001 0.0001 0 0 0.0006 0 0.0001 0.0001 0.0002 0.0003 0.0003 0 0.0002 0 0.0019 0
Bud Neck 0.0042 0.0002 0.0003 0.0002 0.0003 0.001 0.0016 0.0001 0.0003 0.0003 0.001 0.0012 0.0006 0.0001 0.0005 0 0.0208 0.0015
Bud Periphery 0.001 0 0.0001 0.0001 0 0.0001 0.0013 0 0.0002 0.0002 0.0003 0.0006 0.0006 0 0.0004 0 0.0029 0.0001
Bud Site 0.0082 0.0001 0.0001 0.0002 0 0 0.0019 0.0001 0.0004 0.0007 0.0039 0.0009 0.0013 0.0002 0.0007 0 0.0341 0.0001
Cell Periphery 0.0004 0 0 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0.0007 0
Cytoplasm 0.0033 0.0011 0.0023 0.0046 0.0005 0.0012 0.0112 0.0075 0.0011 0.0048 0.0016 0.0009 0.0202 0.021 0.0034 0.0002 0.0011 0.005
Cytoplasmic Foci 0.0075 0.0002 0.0004 0.0034 0 0.0003 0.0274 0.0001 0.0009 0.0046 0.0082 0.0018 0.0077 0.0005 0.0034 0 0.0013 0.0006
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.001 0.0003 0.0004 0.0033 0 0.0002 0.0048 0.0002 0.0006 0.0028 0.0017 0.0006 0.0069 0.001 0.0012 0 0.0005 0.0001
Endosome 0.0143 0.0142 0.0004 0.0096 0.0001 0.0007 0.0276 0.0001 0.0083 0.0062 0.0244 0.0061 0.0119 0.0004 0.0041 0 0.0026 0.0008
Golgi 0.0068 0.0017 0 0.0007 0 0.0004 0.0059 0 0.0021 0.0021 0.0067 0.0033 0.0022 0 0.0007 0 0.0018 0.0006
Lipid Particles 0.0103 0 0 0.0031 0 0.0035 0.0166 0 0.0015 0.0029 0.0112 0.0095 0.0034 0.0001 0.0009 0 0.0016 0.0015
Mitochondria 0.0105 0.001 0.0007 0.0019 0.0002 0.0002 0.0164 0.0003 0.0026 0.001 0.0017 0.0067 0.0015 0.0002 0.0008 0.0001 0.0013 0.0008
None 0.0036 0.0009 0.0062 0.0043 0.0002 0.0004 0.0102 0.0065 0.0014 0.0024 0.0009 0.0004 0.0114 0.0326 0.0054 0 0.0006 0.0092
Nuclear Periphery 0.0091 0.0138 0.0089 0.0066 0.0083 0.0093 0.0331 0.0044 0.0066 0.0151 0.0055 0.0088 0.0207 0.0042 0.0071 0.0006 0.0028 0.0021
Nucleolus 0.0111 0.0077 0.0073 0.0041 0.0234 0.0138 0.0058 0.0051 0.0041 0.0025 0.0183 0.0135 0.004 0.004 0.0052 0.0022 0.015 0.0095
Nucleus 0.8668 0.9509 0.951 0.8778 0.9595 0.9567 0.7587 0.971 0.9541 0.9177 0.8912 0.9311 0.8776 0.9217 0.9429 0.9961 0.8789 0.9553
Peroxisomes 0.0062 0 0 0.033 0 0.0006 0.0209 0 0.0006 0.0013 0.0025 0.0015 0.006 0 0.0035 0 0.0011 0.0033
Punctate Nuclear 0.0165 0.0042 0.0206 0.0421 0.0073 0.0108 0.0176 0.0042 0.0015 0.0025 0.0142 0.0047 0.0103 0.0134 0.0045 0.0008 0.0109 0.0055
Vacuole 0.0057 0.0019 0.0005 0.0034 0.0001 0.0002 0.0054 0.0002 0.0008 0.0004 0.0014 0.001 0.0035 0.0004 0.0012 0 0.0011 0.0002
Vacuole Periphery 0.0018 0.002 0.0002 0.0006 0.0001 0.0001 0.0038 0.0001 0.0007 0.0002 0.0005 0.0008 0.0017 0.0001 0.0004 0 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
354 1823 304 1253 1581 2084 1985 1556 1935 3907 2289 2809

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 591.77 798.74 1017.01 965.66 683.87 819.08 939.29 960.47 667.02 809.59 949.61 962.79
Standard Deviation 80.47 121.40 118.05 141.64 80.70 99.91 120.43 154.35 88.17 110.93 122.98 148.84
Intensity Change Log 2 — 0.432689 0.781225 0.706479 — 0.260282 0.457849 0.490018 — 0.342830 0.616906 0.594484

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000239 0.001199 0.000613 0.000042 0.000079 0.000381 0.000634 0.000046 0.000154 0.000490 0.000625
Bud Neck 0.007060 0.006347 0.008001 0.015822 0.001023 0.006091 0.011989 0.016333 0.002127 0.006210 0.011459 0.016105
Bud Site 0.001205 0.001253 0.001222 0.004368 0.000254 0.003095 0.000888 0.005125 0.000428 0.002235 0.000932 0.004787
Cell Periphery 0.000264 0.000136 0.000091 0.000134 0.000090 0.000156 0.000061 0.000153 0.000121 0.000147 0.000065 0.000145
Cytoplasm 0.169377* 0.076526 0.003728 0.015732 0.079069 0.036268 0.004268 0.017739 0.095591 0.055052 0.004196 0.016843
Cytoplasmic Foci 0.078410 0.004416 0.000108 0.000775 0.007191 0.002511 0.000018 0.000503 0.020220 0.003400 0.000030 0.000625
Eisosomes 0.000096 0.000018 0.000041 0.000021 0.000017 0.000013 0.000015 0.000020 0.000031 0.000015 0.000019 0.000020
Endoplasmic Reticulum 0.001267 0.004012 0.010212 0.006980 0.001033 0.002018 0.004593 0.005447 0.001075 0.002948 0.005340 0.006131
Endosome 0.001733 0.001465 0.000397 0.001312 0.000381 0.000813 0.000170 0.000988 0.000628 0.001117 0.000200 0.001133
Golgi 0.000479 0.000485 0.000080 0.000779 0.000050 0.000182 0.000029 0.000194 0.000129 0.000324 0.000036 0.000455
Lipid Particles 0.009258 0.001134 0.000382 0.000141 0.001027 0.000674 0.000047 0.000226 0.002533 0.000889 0.000092 0.000188
Mitochondria 0.005130 0.003132 0.000981 0.001566 0.000937 0.002924 0.001094 0.001958 0.001704 0.003021 0.001079 0.001783
Mitotic Spindle 0.000124 0.000777 0.000154 0.006699 0.000411 0.002983 0.001698 0.008275 0.000358 0.001954 0.001493 0.007572
None 0.018674 0.011891 0.001672 0.005907 0.008484 0.004629 0.001892 0.006667 0.010348 0.008017 0.001863 0.006328
Nuclear Periphery 0.001079 0.001248 0.000742 0.001350 0.000894 0.001622 0.001185 0.001423 0.000928 0.001448 0.001126 0.001390
Nuclear Periphery Foci 0.000378 0.000813 0.000308 0.000595 0.000287 0.000307 0.000134 0.000426 0.000304 0.000543 0.000157 0.000501
Nucleolus 0.005915 0.004247 0.001835 0.001588 0.002166 0.002581 0.001334 0.001923 0.002852 0.003359 0.001400 0.001774
Nucleus 0.688138* 0.876469* 0.961652* 0.926305* 0.894780* 0.928526* 0.968505* 0.925391* 0.856976* 0.904236* 0.967595* 0.925799*
Peroxisomes 0.004909 0.000945 0.000216 0.000207 0.000332 0.000465 0.000038 0.000181 0.001169 0.000689 0.000062 0.000192
Vacuole 0.006100 0.003954 0.006857 0.007439 0.001426 0.003511 0.001500 0.004771 0.002281 0.003718 0.002211 0.005961
Vacuole Periphery 0.000335 0.000494 0.000123 0.001666 0.000107 0.000551 0.000159 0.001621 0.000149 0.000524 0.000154 0.001641

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.71 -13.18 -18.92 -13.62 5.41 -3.13 -23.13 -16.47 -15.28 -6.66 -9.45 -24.64 -24.96 -20.24 -5.87
Bud Neck 0.45 -1.83 -4.71 -6.93 -4.01 -7.96 -15.75 -15.75 -10.45 -4.58 -7.54 -14.60 -17.46 -12.43 -4.77
Bud Site -0.20 -1.77 -3.67 -3.76 -3.10 -3.87 -6.98 -4.44 -1.20 -3.53 -4.53 -6.48 -6.22 -2.84 -4.95
Cell Periphery 3.29 4.44 3.75 1.25 -1.34 -3.53 3.37 -3.09 0.82 -5.42 -1.81 5.64 -0.47 1.34 -6.12
Cytoplasm 7.92 14.96 13.92 15.96 -6.13 10.26 20.12 15.77 7.41 -8.09 9.65 24.47 20.82 17.02 -9.76
Cytoplasmic Foci 10.58 11.24 11.19 7.28 -1.88 5.31 9.75 9.18 4.43 -2.51 10.63 13.13 12.87 8.25 -3.35
Eisosomes 7.89 4.77 6.87 -6.67 5.71 3.49 -0.42 -5.68 -9.90 -6.98 7.51 4.69 2.61 -11.46 -4.93
Endoplasmic Reticulum -9.02 -12.44 -14.39 -8.74 3.83 -4.63 -19.41 -17.63 -13.45 -4.05 -10.64 -22.59 -22.61 -15.07 -4.06
Endosome 1.02 4.12 2.28 1.33 -1.70 -2.02 3.56 -1.93 0.06 -2.96 -2.42 5.59 -1.16 1.06 -3.98
Golgi 0.04 3.31 0.41 0.33 -1.67 -1.58 1.52 -2.05 0.24 -2.35 -2.08 3.70 -1.49 0.45 -2.55
Lipid Particles 6.31 6.98 7.11 3.81 3.10 2.16 8.61 6.29 3.43 -2.75 5.72 9.33 8.79 5.04 -3.31
Mitochondria 1.57 3.96 3.29 1.68 -4.99 -2.76 -0.72 -2.85 1.28 -4.24 -2.19 1.25 -0.76 2.09 -4.89
Mitotic Spindle -2.54 -1.32 -3.65 -3.18 -2.07 -2.73 -2.20 -4.68 -2.79 -3.29 -2.81 -2.47 -5.75 -4.14 -3.67
None 1.62 4.29 3.45 5.43 -4.13 3.27 6.48 2.33 -0.91 -4.62 1.82 7.64 4.27 3.56 -5.77
Nuclear Periphery -1.15 0.30 -4.98 -4.15 -5.62 -2.94 -1.60 -5.23 -0.87 -2.10 -3.59 -1.52 -7.75 -2.69 -3.03
Nuclear Periphery Foci -1.25 0.81 -0.80 0.78 -1.52 -0.03 1.08 -0.71 -0.68 -2.57 -1.26 1.32 -1.27 0.41 -3.51
Nucleolus 1.47 3.12 3.13 4.70 0.25 -1.29 3.07 -0.27 0.96 -2.38 -1.23 4.30 2.43 3.94 -1.84
Nucleus -11.32 -15.99 -14.34 -7.42 4.60 -5.81 -14.07 -4.22 1.62 10.02 -8.08 -19.71 -11.25 -4.21 11.94
Peroxisomes 4.44 5.72 5.79 2.41 1.09 -1.26 4.76 2.51 3.25 -2.29 2.02 6.77 6.15 3.32 -2.14
Vacuole 1.86 -2.36 -3.48 -5.31 -0.24 -3.02 -2.69 -7.41 -3.43 -4.91 -3.10 -2.95 -9.27 -6.24 -6.23
Vacuole Periphery -1.27 4.66 -1.20 -0.82 -1.68 -2.30 -0.53 -1.79 -0.88 -1.70 -3.20 0.04 -2.33 -1.19 -2.31
Standard name
Human Ortholog
Description Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rgm1

Rgm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available