Standard name
Human Ortholog
Description Protein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ATG41, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.31 0.39 0.78 0.61 0.58 0.66 0.71 0.69 0.69 0.62 0.37 0.47 0.64 0.54 0.56 0.52 0 0 0.12 0 0 0
Endoplasmic Reticulum 0 0 0.06 0.07 0 0.07 0 0 0 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0.09 0.2 0.14 0.16 0.15 0.14 0.17 0.2 0.13 0.2 0.27 0.32
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.44 0.15 0.14 0.42 0.42 0.47 0.39 0.43 0.42 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Nuclear Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.65 0.22 0.15 0.28 0.18 0.11 0.11 0.11 0 0.05 0.61 0.49 0.39 0.39 0.36 0.48 0.69 0.69 0.58 0.72 0.58 0.48
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 1 0 1 1 1 1 1 1 2 0 0 0 0 0 0 1 0
Bud 1 0 0 0 1 0 6 3 7 0 6 0 2 2 0 2 0 0 0 1 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 1 2 3 2 2 9 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 98 36 7 47 111 76 189 106 104 60 100 56 130 202 62 59 79 7 2 7 0 6 6
Endoplasmic Reticulum 7 4 1 4 6 9 0 1 2 7 5 3 4 2 4 5 4 0 0 1 0 0 0
Endosome 10 3 0 1 13 0 1 0 0 0 0 14 54 44 18 16 21 47 27 8 5 54 77
Golgi 0 2 0 0 0 0 0 0 0 0 0 2 0 0 1 0 1 1 0 0 0 0 1
Mitochondria 25 5 8 9 26 54 119 70 59 37 68 3 2 1 3 4 4 4 1 4 0 4 7
Nucleus 3 2 0 0 0 1 1 2 0 0 2 1 3 1 0 2 0 6 3 0 0 3 12
Nuclear Periphery 7 5 1 0 1 3 0 1 0 0 0 1 1 0 0 0 0 15 3 1 0 4 2
Nucleolus 3 4 0 0 2 0 0 0 2 0 0 6 7 5 1 0 1 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 1 0 0 0 4 1 7 5 1 2 1 0 1 0 3 9
Vac/Vac Membrane 139 76 4 9 50 24 31 16 17 1 8 93 134 125 44 38 73 200 92 36 20 116 114
Unique Cell Count 247 117 18 60 181 130 286 149 150 87 162 153 276 317 114 105 153 290 135 63 28 201 240
Labelled Cell Count 293 138 21 70 213 168 351 203 194 108 200 184 340 391 140 128 186 290 135 63 28 201 240


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.5 4.6 5.7 6.1 4.6 4.4 6.3 4.9 4.4 4.7 8.2 8.8 7.8 8.7 8.6 8.4 6.6 7.0
Std Deviation (1e-4) 1.0 1.1 0.6 0.8 1.3 0.6 0.9 19.8 2.0 0.7 2.8 1.4 2.4 1.4 2.0 1.6 1.6 1.2 1.5
Intensity Change (Log2) 0.32 0.43 0.02 -0.04 0.45 0.09 -0.05 0.04 0.84 0.95 0.77 0.93 0.91 0.87 0.52 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.8 1.6 2.3 2.8 2.6 2.4 1.9 -0.2 0.7 2.1 1.2 1.4 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.2 -0.4 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 3.1 2.2 1.5 1.3 1.2 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6507 5.1182 4.0423 4.0087 3.3696 4.369 3.0336 4.7834 4.1002 3.4145 2.9112 3.9165 5.5956 6.6427 5.8317 5.412 4.6297 5.9034
Actin 0.0012 0.0001 0.0015 0.011 0.005 0.0002 0.0155 0.0003 0.0062 0.0064 0.0023 0.0007 0.0054 0.0109 0.0076 0.0289 0.0157 0.0006
Bud 0.0007 0.0007 0.001 0.0191 0.0051 0.0015 0.001 0.0016 0.0018 0.002 0.0016 0.0031 0.0027 0.0005 0.0019 0.0025 0.0092 0.0012
Bud Neck 0.0005 0.0001 0.0085 0.0012 0.0019 0.0009 0.0006 0.0011 0.0008 0.0005 0.0009 0.0006 0.0007 0.0003 0.008 0.0006 0.0006 0.001
Bud Periphery 0.0008 0.0011 0.0006 0.0118 0.0053 0.0014 0.0009 0.0013 0.0014 0.0012 0.0012 0.0023 0.0043 0.0002 0.0011 0.0076 0.0084 0.0009
Bud Site 0.0009 0.0003 0.0013 0.0044 0.0038 0.0005 0.0041 0.0059 0.0041 0.0023 0.0059 0.0021 0.0027 0.001 0.0015 0.0014 0.0053 0.001
Cell Periphery 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0003 0.0002 0.0002 0.0003 0.0001 0.0004 0.0001 0.0002 0.0002 0.0005 0.0001
Cytoplasm 0.1243 0.3107 0.2627 0.2719 0.096 0.1954 0.2113 0.3824 0.2994 0.1373 0.1809 0.24 0.1566 0.2858 0.2725 0.1357 0.1117 0.1675
Cytoplasmic Foci 0.0441 0.01 0.0644 0.029 0.0598 0.0316 0.0659 0.0416 0.0653 0.0264 0.0327 0.0322 0.0611 0.0639 0.0783 0.0291 0.0364 0.0631
Eisosomes 0 0 0.0001 0 0.0001 0 0.0002 0 0 0.0002 0 0 0.0001 0 0.0001 0.0003 0.0003 0
Endoplasmic Reticulum 0.0105 0.0398 0.0168 0.0082 0.0119 0.0132 0.0149 0.0195 0.0079 0.0131 0.0058 0.0112 0.0105 0.0136 0.0104 0.0061 0.0053 0.0111
Endosome 0.3673 0.1968 0.327 0.2911 0.3696 0.3208 0.3576 0.2844 0.3901 0.3767 0.3582 0.372 0.3446 0.2454 0.346 0.3299 0.3265 0.3486
Golgi 0.0129 0.0037 0.0167 0.0092 0.0375 0.0083 0.0178 0.0084 0.0191 0.0373 0.0609 0.0086 0.0092 0.0069 0.013 0.0125 0.0195 0.0107
Lipid Particles 0.004 0.0001 0.0077 0.004 0.0291 0.0035 0.0182 0.001 0.0047 0.0108 0.0042 0.0024 0.0088 0.0069 0.0091 0.0057 0.0109 0.002
Mitochondria 0.0074 0.0102 0.0148 0.0045 0.0487 0.0081 0.0063 0.0146 0.0102 0.0413 0.0118 0.0039 0.0087 0.0017 0.0071 0.0085 0.0051 0.0065
None 0.0063 0.0026 0.01 0.001 0.0007 0.0007 0.0103 0.0036 0.001 0.0012 0.0006 0.001 0.0089 0.0012 0.0045 0.0013 0.0075 0.0015
Nuclear Periphery 0.0427 0.0294 0.018 0.0197 0.0317 0.044 0.0326 0.0216 0.0085 0.0386 0.0119 0.0293 0.0453 0.0203 0.0161 0.0091 0.0179 0.0337
Nucleolus 0.0004 0.0001 0.001 0.0002 0.0006 0.0003 0.0004 0.0004 0.0005 0.0052 0.0005 0.0004 0.0008 0.0002 0.0005 0.0002 0.0005 0.0007
Nucleus 0.0122 0.0047 0.0064 0.0047 0.0039 0.0116 0.0082 0.0071 0.0041 0.0161 0.0057 0.0122 0.0173 0.0043 0.014 0.0028 0.0029 0.0152
Peroxisomes 0.0031 0.0001 0.0107 0.0422 0.0119 0.0022 0.0037 0.0005 0.0033 0.0021 0.0021 0.0022 0.005 0.0075 0.0093 0.0079 0.0085 0.0022
Punctate Nuclear 0.004 0.0003 0.0033 0.0012 0.0014 0.001 0.0024 0.0009 0.0019 0.0121 0.0004 0.0076 0.0041 0.0046 0.0098 0.0006 0.0105 0.0022
Vacuole 0.2468 0.2388 0.1377 0.1753 0.1643 0.2051 0.1671 0.1435 0.12 0.1648 0.2187 0.1759 0.2158 0.2232 0.1196 0.2508 0.2436 0.1962
Vacuole Periphery 0.1096 0.1502 0.0896 0.0903 0.1115 0.1497 0.0607 0.06 0.0494 0.1043 0.0934 0.0922 0.087 0.1016 0.0695 0.1582 0.1531 0.1341

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4938 17.9808 24.779 42.3373 39.1183 31.5904 52.5933 49.9586 77.3189 58.3569
Translational Efficiency 4.3072 1.9352 1.507 1.3355 1.7245 2.7418 1.4368 2.1119 1.3372 1.3033

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1382 1651 188 163 1035 1259 1839 1362 2417 2910 2027 1525

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 813.26 887.61 1129.55 905.64 712.57 872.51 854.98 881.83 770.14 881.08 880.45 884.37
Standard Deviation 117.18 136.91 144.07 109.13 97.14 122.15 155.17 111.25 119.89 130.94 173.53 111.27
Intensity Change Log 2 0.126209 0.473960 0.155221 0.292140 0.262859 0.307469 0.206080 0.379203 0.228325

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000316 0.000241 0.000713 0.000119 0.000279 0.000077 0.000194 0.000241 0.000300 0.000170 0.000242 0.000228
Bud Neck 0.004980 0.004017 0.015392 0.024123 0.006646 0.003610 0.011441 0.017074 0.005694 0.003841 0.011807 0.017827
Bud Site 0.010953 0.015518 0.026827 0.013725 0.017656 0.007717 0.019408 0.026475 0.013823 0.012143 0.020096 0.025112
Cell Periphery 0.000110 0.000071 0.000135 0.000073 0.000367 0.000093 0.000059 0.000050 0.000220 0.000081 0.000066 0.000052
Cytoplasm 0.186837* 0.050964 0.026032 0.115341* 0.108124* 0.024812 0.068194 0.123822* 0.153131* 0.039649 0.064283 0.122916*
Cytoplasmic Foci 0.183302* 0.121114 0.034798 0.034651 0.206479* 0.097010 0.040410 0.063459 0.193226* 0.110686 0.039889 0.060380
Eisosomes 0.000029 0.000014 0.000040 0.000057 0.000102 0.000021 0.000018 0.000022 0.000060 0.000017 0.000020 0.000026
Endoplasmic Reticulum 0.007816 0.003036 0.004741 0.003464 0.020483 0.003084 0.002656 0.003029 0.013240 0.003056 0.002849 0.003075
Endosome 0.127359 0.168741 0.089310 0.106612 0.123543 0.222440* 0.101853 0.146021 0.125725 0.191974 0.100690 0.141809
Golgi 0.007398 0.026507 0.015303 0.000462 0.015784 0.023304 0.009511 0.003912 0.010989 0.025121 0.010048 0.003543
Lipid Particles 0.007108 0.002213 0.002633 0.004086 0.011255 0.003741 0.001238 0.002697 0.008884 0.002874 0.001367 0.002846
Mitochondria 0.009941 0.020646 0.007265 0.001095 0.067259 0.028055 0.009274 0.004166 0.034486 0.023852 0.009087 0.003837
Mitotic Spindle 0.001917 0.015284 0.059901 0.008048 0.000563 0.008581 0.023179 0.008578 0.001337 0.012384 0.026585 0.008522
None 0.000703 0.000358 0.000267 0.001479 0.001162 0.001105 0.001140 0.000307 0.000900 0.000682 0.001059 0.000432
Nuclear Periphery 0.001381 0.001509 0.007016 0.002583 0.000706 0.001501 0.003030 0.001964 0.001092 0.001506 0.003399 0.002030
Nuclear Periphery Foci 0.002739 0.002057 0.009488 0.002413 0.000479 0.002317 0.003887 0.002298 0.001771 0.002169 0.004407 0.002310
Nucleolus 0.001597 0.001049 0.008816 0.007061 0.000404 0.001693 0.007801 0.002520 0.001086 0.001328 0.007895 0.003005
Nucleus 0.018121 0.003932 0.027130 0.077310 0.007967 0.003489 0.024778 0.025026 0.013773 0.003740 0.024996 0.030615
Peroxisomes 0.002286 0.003295 0.005836 0.006139 0.002559 0.003099 0.002685 0.004740 0.002403 0.003210 0.002978 0.004890
Vacuole 0.329477* 0.356276* 0.463081* 0.574905* 0.221411* 0.338472* 0.528249* 0.537836* 0.283201* 0.348573* 0.522205* 0.541798*
Vacuole Periphery 0.095630 0.203159* 0.195276* 0.016255 0.186772* 0.225780* 0.140996* 0.025763 0.134659* 0.212946* 0.146030* 0.024746

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.42 -2.32 0.99 1.23 5.08 3.48 1.06 0.67 -3.61 -0.36 1.26 0.35 0.77 -0.95 1.01
Bud Neck 1.56 -6.59 -3.32 -3.53 -0.69 3.36 -6.26 -8.23 -10.49 -3.60 3.57 -9.58 -10.09 -11.71 -3.63
Bud Site -3.49 -5.68 -0.51 1.15 3.94 6.77 -1.26 -2.52 -7.54 -1.52 1.58 -4.90 -4.47 -5.28 -0.78
Cell Periphery 3.55 -0.96 3.04 -0.11 2.25 7.46 9.02 9.39 3.19 2.87 8.13 9.54 10.77 4.07 3.54
Cytoplasm 19.82 19.74 4.06 -4.51 -5.83 15.56 5.03 -5.24 -20.19 -10.36 24.60 14.96 1.22 -18.34 -11.38
Cytoplasmic Foci 10.96 26.91 26.36 17.68 0.35 16.66 29.01 24.92 9.47 -7.01 19.19 40.91 34.40 17.36 -6.71
Eisosomes 7.87 -2.91 -4.13 -6.35 -1.71 9.47 9.97 9.43 -1.02 -3.84 11.06 10.23 8.57 -6.39 -4.25
Endoplasmic Reticulum 7.82 3.30 6.55 -0.77 2.13 9.24 9.45 9.32 0.55 -0.13 11.47 11.64 11.31 0.39 0.28
Endosome -6.61 4.85 3.73 7.08 0.30 -13.00 5.31 0.39 12.72 -4.36 -13.64 7.37 1.65 12.86 -4.06
Golgi -11.10 -2.38 10.58 16.57 4.95 -3.84 5.44 10.53 12.16 8.01 -10.90 1.84 11.15 18.58 9.36
Lipid Particles 7.69 4.54 4.18 -3.18 -1.12 5.91 8.70 7.41 1.90 -5.96 9.17 12.31 9.75 0.26 -6.06
Mitochondria -5.22 1.49 7.40 11.79 3.76 8.56 14.07 15.33 10.95 4.99 4.53 12.37 14.95 13.82 5.57
Mitotic Spindle -7.70 -5.96 -2.23 -0.64 3.09 -5.15 -11.52 -7.11 -0.52 6.29 -9.24 -12.57 -6.48 0.84 6.84
None 2.56 3.95 -0.83 -1.30 -1.41 0.05 0.06 1.02 0.96 2.40 0.46 -0.31 1.23 0.60 1.94
Nuclear Periphery -0.45 -2.82 -2.76 -2.40 2.11 -2.27 -8.11 -5.65 -1.78 2.74 -1.66 -7.00 -4.37 -2.49 3.41
Nuclear Periphery Foci 1.13 -2.95 -1.41 -2.48 2.39 -3.23 -6.78 -4.08 -0.35 2.58 -0.86 -4.95 -1.67 -1.04 3.21
Nucleolus 1.60 -3.88 -2.67 -3.07 1.54 -2.31 -10.43 -7.41 -1.45 6.81 -0.81 -10.03 -5.45 -3.88 6.89
Nucleus 9.79 -2.11 -4.75 -6.01 -3.18 4.09 -10.28 -11.51 -14.44 -1.80 10.47 -7.85 -10.11 -15.27 -3.17
Peroxisomes -1.74 -3.24 -1.87 -1.05 0.71 -0.95 -0.20 -3.24 -1.32 -3.18 -1.95 -1.91 -4.10 -1.64 -2.73
Vacuole -3.15 -8.04 -11.03 -9.96 -4.68 -13.15 -36.08 -33.04 -20.93 -1.93 -10.31 -33.30 -31.14 -24.01 -3.29
Vacuole Periphery -16.73 -6.11 18.36 33.28* 12.14 -4.47 7.60 25.99* 33.77* 29.40* -14.71 -0.50 30.48 47.33* 31.74*
Standard name
Human Ortholog
Description Protein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ATG41, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (34%), cytoplasm (16%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Icy1

Icy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Icy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available