Standard name
Human Ortholog
Description Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0.1 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0.06 0 0 0.06 0 0 0 – 0.05 0.07 0.08 0.08 0.08 0 0 0 0 0.07 0.18 0 0 0
Cytoplasm 0.94 0.98 0.97 0.99 0.95 0.88 0.85 0.83 0.72 – 0.62 0.5 0.97 0.98 0.98 0.94 0.93 0.97 0.64 0.77 0.46 0.9 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.22 0.23 0.26 0.42 – 0.49 0.63 0 0 0 0.05 0 0 0.09 0 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 0 1 1 0 1 2 2 – 3 3 3 3 2 0 0 1 16 16 21 2 1 3
Bud 2 1 5 3 3 10 7 11 8 – 16 14 2 3 10 0 0 0 1 0 1 1 2 8
Bud Neck 1 1 5 1 0 1 4 3 0 – 0 1 0 3 0 6 4 4 6 11 8 0 0 2
Bud Site 0 0 0 0 0 2 0 1 2 – 4 0 0 0 1 0 0 0 – – – – – –
Cell Periphery 6 25 14 6 9 16 7 7 13 – 15 22 17 22 27 6 9 10 9 25 38 2 1 2
Cytoplasm 190 336 218 197 257 234 365 463 246 – 200 151 202 262 317 346 230 387 144 272 97 136 168 252
Endoplasmic Reticulum 5 2 0 0 1 0 0 0 3 – 2 8 2 1 2 39 9 23 8 3 4 1 0 3
Endosome 1 0 0 0 1 1 1 0 0 – 0 0 0 0 1 4 5 6 7 2 2 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 5 0 1 4 1 2 1 0 3
Mitochondria 0 5 1 7 17 60 101 147 144 – 157 191 0 0 9 17 4 4 20 12 28 2 3 5
Nucleus 0 0 0 0 0 2 3 4 0 – 1 2 0 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 3 5 0 5 – 7 3 0 1 0 1 0 0 1 0 0 0 0 0
SpindlePole 0 1 1 0 2 4 8 14 10 – 6 3 0 2 0 2 3 1 1 0 0 0 0 1
Vac/Vac Membrane 2 0 1 0 0 1 1 0 0 – 0 0 0 0 0 9 8 4 3 5 3 1 1 2
Unique Cell Count 202 343 224 199 271 267 430 557 340 320 304 209 268 325 370 246 399 226 355 215 151 185 291
Labelled Cell Count 210 372 245 216 293 334 504 653 434 412 398 226 297 369 437 274 441 226 355 215 151 185 291


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.2 4.0 3.8 4.0 3.7 3.5 3.6 3.2 – 3.3 3.0 5.0 4.9 4.2 5.9 6.7 6.4 4.9 4.9 5.4
Std Deviation (1e-4) 0.6 0.7 0.9 1.3 1.2 1.2 1.2 1.3 0.8 – 1.2 0.7 1.2 1.4 1.2 2.3 3.2 2.8 1.1 1.4 1.4
Intensity Change (Log2) – – – -0.09 -0.0 -0.14 -0.19 -0.14 -0.34 – -0.29 -0.44 0.33 0.29 0.08 0.55 0.73 0.66 0.3 0.3 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5313 1.7288 1.5554 1.5432 1.1487 1.4912 3.8016 2.2932 2.0074 2.2374 1.4506 1.6647 2.855 2.2502 2.4206 2.1388 1.9058 2.1806
Actin 0.0512 0.0044 0.0073 0.0018 0.0014 0.0126 0.141 0.0132 0.0065 0.0212 0.0088 0.0007 0.0303 0.0102 0.019 0.0118 0.0082 0.0087
Bud 0.0023 0.0036 0.0059 0.0006 0.0005 0.0008 0.0024 0.0012 0.0015 0.0019 0.0133 0.0002 0.0011 0.0007 0.0002 0.0006 0.0017 0.0002
Bud Neck 0.0033 0.001 0.0101 0.003 0.0004 0.006 0.0146 0.0004 0.0003 0.001 0.0012 0.0006 0.0004 0.0009 0.0002 0.0023 0.0031 0.0012
Bud Periphery 0.0052 0.0039 0.0067 0.0025 0.0027 0.0021 0.0039 0.0021 0.0016 0.0035 0.0256 0.0004 0.0018 0.0015 0.0004 0.0023 0.0021 0.0005
Bud Site 0.0033 0.0274 0.0128 0.0008 0.0002 0.0005 0.008 0.0096 0.0147 0.0024 0.0051 0 0.0032 0.0137 0.0018 0.0072 0.003 0.0002
Cell Periphery 0.0053 0.0056 0.007 0.0045 0.0013 0.0021 0.0025 0.0083 0.0012 0.001 0.0014 0.0002 0.0138 0.0058 0.003 0.0078 0.0044 0.0013
Cytoplasm 0.257 0.3944 0.3851 0.2831 0.3873 0.4724 0.2317 0.4029 0.3771 0.4366 0.405 0.5056 0.5062 0.3661 0.3101 0.3201 0.5128 0.4755
Cytoplasmic Foci 0.0159 0.0076 0.0121 0.0328 0.0037 0.0113 0.0228 0.0081 0.0135 0.0057 0.0142 0.0027 0.0042 0.0067 0.0033 0.0095 0.0447 0.0054
Eisosomes 0.0017 0.001 0.001 0.0006 0.0003 0.0003 0.0019 0.0005 0.0002 0.0003 0.0001 0.0001 0.0005 0.0009 0.0003 0.0012 0.0002 0.0002
Endoplasmic Reticulum 0.0061 0.0041 0.0063 0.0018 0.0026 0.0041 0.0217 0.003 0.003 0.0071 0.0033 0.0018 0.0027 0.0024 0.0024 0.005 0.0043 0.0023
Endosome 0.0121 0.0012 0.0042 0.0053 0.0125 0.0056 0.0512 0.0028 0.0126 0.0475 0.0295 0.0006 0.004 0.0006 0.0006 0.0013 0.0179 0.0022
Golgi 0.004 0.0006 0.0009 0.0008 0.0008 0.0021 0.0155 0.0029 0.0177 0.0251 0.0086 0.0001 0.0029 0.0008 0.0011 0.0008 0.0078 0.0026
Lipid Particles 0.0071 0.0016 0.0021 0.0038 0.0013 0.0044 0.0213 0.0023 0.0041 0.0074 0.0005 0.0002 0.0016 0.0009 0.0006 0.0024 0.007 0.0008
Mitochondria 0.0021 0.0006 0.001 0.001 0.0052 0.0017 0.0354 0.0015 0.0051 0.0676 0.0117 0.0001 0.002 0.0003 0.0002 0.0004 0.012 0.0088
None 0.5881 0.5335 0.5279 0.6494 0.5368 0.452 0.2201 0.535 0.5335 0.354 0.4633 0.4858 0.4166 0.5853 0.6542 0.6237 0.3589 0.4881
Nuclear Periphery 0.0116 0.0005 0.0009 0.0003 0.0109 0.006 0.0915 0.002 0.0003 0.003 0.001 0.0002 0.0012 0.0003 0.0003 0.0005 0.0008 0.0005
Nucleolus 0.0011 0.001 0.0013 0.0003 0.001 0.0032 0.0025 0.0001 0.0001 0.0004 0.0002 0 0.0003 0.0003 0.0001 0.0002 0.0002 0
Nucleus 0.0047 0.002 0.0026 0.0004 0.0034 0.0057 0.0695 0.0004 0.0002 0.0011 0.001 0.0002 0.0007 0.0005 0.0004 0.0004 0.0009 0.0002
Peroxisomes 0.0053 0.0004 0.0008 0.005 0.0002 0.0013 0.013 0.0018 0.0055 0.0022 0.0024 0 0.0018 0.0005 0.0009 0.0009 0.0019 0.0005
Punctate Nuclear 0.0075 0.0043 0.0021 0.001 0.0003 0.0027 0.0151 0.0005 0.0003 0.0008 0.0006 0.0002 0.003 0.0007 0.0004 0.0003 0.0058 0.0002
Vacuole 0.0038 0.0011 0.0018 0.0011 0.0124 0.0023 0.0065 0.0009 0.0007 0.0064 0.0022 0.0003 0.0015 0.0008 0.0005 0.0012 0.0016 0.0005
Vacuole Periphery 0.0012 0.0001 0.0002 0.0001 0.0149 0.0008 0.0079 0.0003 0.0002 0.0038 0.0009 0 0.0002 0.0001 0 0.0001 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.2887 4.5581 7.8921 11.4218 8.8146 8.1537 13.7493 12.857 13.7472 12.8494
Translational Efficiency 0.6277 0.9461 0.5037 0.3783 0.4082 0.7389 0.4622 0.4822 0.4527 0.4399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
107 55 1359 8 1172 1940 1697 2217 1279 1995 3056 2225

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 669.94 320.81 987.07 998.65 653.51 700.62 809.20 864.51 654.88 690.15 888.30 864.99
Standard Deviation 67.08 7.21 108.77 107.68 77.54 86.75 89.31 100.62 76.86 105.77 132.30 100.96
Intensity Change Log 2 — -1.062313 0.559121 0.575947 — 0.100423 0.308287 0.403673 — -0.373713 0.440701 0.493448

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000230 0.000016 0.000374 0.000346 0.000243 0.000759 0.001744 0.002931 0.000241 0.000739 0.001135 0.002921
Bud Neck 0.043305 0.000008 0.004071 0.002690 0.018370 0.045127 0.006489 0.008558 0.020456 0.043883 0.005414 0.008537
Bud Site 0.008895 0.000017 0.017023 0.006757 0.002956 0.014002 0.019661 0.028078 0.003453 0.013617 0.018488 0.028001
Cell Periphery 0.000552 0.000059 0.000386 0.000461 0.000278 0.000440 0.000450 0.000941 0.000301 0.000429 0.000422 0.000939
Cytoplasm 0.449526* 0.000037 0.633306* 0.275882* 0.511592* 0.475444* 0.614875* 0.534344* 0.506400* 0.462337* 0.623071* 0.533415*
Cytoplasmic Foci 0.287317* 0.000014 0.047499 0.102984 0.148034 0.216817* 0.060548 0.033564 0.159687 0.210840* 0.054745 0.033813
Eisosomes 0.002401 0.000029 0.000106 0.000106 0.000790 0.001227 0.000252 0.000550 0.000925 0.001194 0.000187 0.000548
Endoplasmic Reticulum 0.000241 0.000001 0.002546 0.002304 0.000910 0.000717 0.000756 0.001604 0.000854 0.000697 0.001552 0.001607
Endosome 0.009385 0.000001 0.003997 0.098990 0.006580 0.021430 0.003912 0.003780 0.006815 0.020840 0.003950 0.004123
Golgi 0.002148 0.000005 0.000190 0.021683 0.002941 0.007497 0.000339 0.001487 0.002874 0.007291 0.000273 0.001560
Lipid Particles 0.003683 0.000022 0.002272 0.001591 0.015828 0.010957 0.001537 0.001341 0.014812 0.010656 0.001864 0.001342
Mitochondria 0.001455 0.000009 0.000152 0.018701 0.002449 0.008072 0.001010 0.003234 0.002366 0.007849 0.000628 0.003290
Mitotic Spindle 0.000021 0.000348 0.001417 0.001892 0.000149 0.002051 0.006817 0.017593 0.000138 0.002005 0.004416 0.017537
None 0.001430 0.999279* 0.001463 0.000308 0.002365 0.001922 0.001354 0.001641 0.002287 0.029418 0.001403 0.001636
Nuclear Periphery 0.000075 0.000000 0.000649 0.000894 0.000277 0.000224 0.000383 0.000538 0.000260 0.000218 0.000502 0.000540
Nuclear Periphery Foci 0.000316 0.000053 0.003166 0.003643 0.000213 0.000340 0.000746 0.000693 0.000222 0.000333 0.001822 0.000704
Nucleolus 0.000248 0.000039 0.000161 0.000186 0.000472 0.000572 0.000198 0.000130 0.000453 0.000558 0.000181 0.000130
Nucleus 0.063026 0.000012 0.138992* 0.057231 0.236490* 0.091251 0.126780 0.192673* 0.221978* 0.088736 0.132211* 0.192186*
Peroxisomes 0.003314 0.000049 0.000509 0.001028 0.004538 0.009856 0.001726 0.000998 0.004436 0.009586 0.001185 0.000998
Vacuole 0.122126 0.000001 0.141541 0.346080* 0.044269 0.090114 0.149962 0.164216 0.050783 0.087630 0.146217 0.164870
Vacuole Periphery 0.000306 0.000000 0.000181 0.056246 0.000256 0.001179 0.000462 0.001105 0.000260 0.001147 0.000337 0.001303

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NAN NAN NAN NAN NAN -5.34 -2.07 -3.38 -2.36 -0.76 -5.29 -2.29 -3.38 -2.40 -1.70
Bud Neck NAN NAN NAN NAN NAN -9.80 7.13 6.51 16.78 -1.98 -8.72 9.11 7.72 16.63 -4.19
Bud Site NAN NAN NAN NAN NAN -8.29 -7.43 -9.89 -3.00 -1.42 -7.83 -9.35 -9.62 -3.20 -1.92
Cell Periphery NAN NAN NAN NAN NAN -5.29 -3.33 -4.14 -2.31 -2.72 -4.24 -3.01 -3.89 -2.42 -3.01
Cytoplasm NAN NAN NAN NAN NAN 4.02 -6.24 4.92 1.04 11.57 5.02 -8.54 4.57 -0.44 14.12
Cytoplasmic Foci NAN NAN NAN NAN NAN -10.46 16.74 23.30 42.65 11.02 -7.86 21.03 25.84 41.60 11.67
Eisosomes NAN NAN NAN NAN NAN -3.30 8.06 3.14 5.23 -1.30 -1.70 6.62 3.41 5.11 -1.79
Endoplasmic Reticulum NAN NAN NAN NAN NAN 1.28 0.10 -5.92 -10.59 -8.97 1.12 -6.13 -6.63 -10.87 -0.97
Endosome NAN NAN NAN NAN NAN -11.02 3.91 4.69 15.73 1.83 -10.85 4.42 4.96 15.29 1.89
Golgi NAN NAN NAN NAN NAN -5.64 6.44 4.21 8.86 -2.05 -5.69 6.95 4.20 8.73 -2.37
Lipid Particles NAN NAN NAN NAN NAN 2.88 9.08 9.14 13.50 0.52 2.65 8.94 9.23 13.41 3.57
Mitochondria NAN NAN NAN NAN NAN -4.01 2.08 0.49 4.75 -2.26 -4.08 2.62 0.39 4.67 -3.05
Mitotic Spindle NAN NAN NAN NAN NAN -2.61 -4.63 -6.93 -5.39 -2.35 -2.63 -5.19 -6.94 -5.44 -4.04
None NAN NAN NAN NAN NAN 0.54 1.74 1.50 0.73 -0.26 -7.25 1.84 1.48 7.53 -0.35
Nuclear Periphery NAN NAN NAN NAN NAN 1.59 -5.87 -12.39 -14.18 -7.58 1.36 -11.42 -13.03 -14.40 -5.74
Nuclear Periphery Foci NAN NAN NAN NAN NAN -0.99 -8.98 -7.10 -3.61 -0.64 -0.89 -16.62 -7.26 -3.84 8.49
Nucleolus NAN NAN NAN NAN NAN -1.08 4.56 7.75 5.11 1.81 -1.17 5.92 7.88 5.07 1.86
Nucleus NAN NAN NAN NAN NAN 23.80 15.12 5.65 -22.29 -11.33 22.83 13.37 3.74 -22.86 -11.19
Peroxisomes NAN NAN NAN NAN NAN -6.56 5.49 7.74 14.14 3.05 -6.66 7.16 8.12 14.08 1.93
Vacuole NAN NAN NAN NAN NAN -12.42 -25.74 -34.56 -25.89 -7.38 -9.79 -29.83 -33.22 -26.55 -9.04
Vacuole Periphery NAN NAN NAN NAN NAN -6.88 -0.92 -1.76 2.01 -1.09 -6.79 -0.30 -1.99 1.44 -1.83
Standard name
Human Ortholog
Description Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (37%), ER (0%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pkh2

Pkh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pkh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available