Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0.15 0.15 0.1 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.09 0 – 0.15 0.06 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.87 0.89 0.93 0.87 0.82 0.82 – 0.74 0.74 0.74 0.74 0.84 0.75 0.77 0.78 0.84 0.79 0.76 0.84 0.76 0.63 0.6 0.61
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.27 0.13 0.13 0.07 0.11 0.14 0.17 – 0.31 0.32 0.26 0.32 0.19 0.16 0.12 0.12 0.07 0.11 0.17 0.07 0.18 0.29 0.29 0.24
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1
Bud 0 1 0 0 0 0 1 – 1 2 4 6 0 0 1 0 0 0 3 1 2 1 5 6
Bud Neck 0 0 0 0 0 0 2 – 1 2 1 6 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 1 0 0 0 4 7 3 – 20 13 20 21 0 2 1 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 5 8 11 14 17 16 18 – 11 16 12 9 28 76 94 18 21 24 0 2 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 2 0 3 0 0 0 0 2 0
Endosome 2 2 5 0 5 2 4 – 1 6 5 1 1 4 7 1 0 0 1 5 4 4 3 9
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 1 0 1 1 2 0 3 2 2 2 3 4
Mitochondria 1 21 1 5 6 40 10 – 103 38 91 79 0 1 1 7 3 9 0 3 1 1 3 7
Nucleus 312 321 376 387 355 375 556 – 516 510 428 599 291 387 494 141 168 164 282 345 353 256 346 356
Nuclear Periphery 1 0 4 3 1 5 6 – 8 5 3 7 1 3 2 0 1 0 0 0 0 0 2 1
Nucleolus 95 48 55 27 43 64 116 – 217 220 152 258 65 81 75 22 14 22 64 30 82 120 167 142
Peroxisomes 1 0 0 0 0 0 0 – 0 1 0 0 0 5 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 3 1 5 4 21 – 2 15 8 9 1 6 15 4 2 2 1 2 3 4 6 12
Vac/Vac Membrane 2 9 2 2 9 11 10 – 3 5 4 6 2 6 5 4 4 2 11 13 7 14 31 36
Unique Cell Count 352 370 421 414 407 455 675 693 689 578 813 346 515 643 181 200 207 373 410 463 409 581 589
Labelled Cell Count 420 411 457 440 446 524 747 883 833 728 1001 390 571 702 201 216 227 373 410 463 409 581 589


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 7.6 8.7 8.4 8.3 6.4 7.1 – 6.2 6.6 6.3 6.3 9.1 8.7 9.0 9.2 10.4 8.6 8.0 8.4 8.7
Std Deviation (1e-4) 1.3 1.4 1.7 1.8 1.7 1.8 1.6 – 1.3 1.3 1.4 1.3 2.8 2.5 2.6 2.3 2.1 2.0 2.0 1.9 2.2
Intensity Change (Log2) – – – -0.06 -0.08 -0.44 -0.29 – -0.49 -0.41 -0.47 -0.47 0.07 -0.0 0.04 0.07 0.25 -0.01 -0.12 -0.06 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 3.5 0 3.9 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 1.2 0.8 0.1 – -1.2 -0.3 -0.6 -2.0 3.4 6.4 6.4 3.9 4.2 4.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.0 0 – 8.1 4.6 8.4 6.4 0 0 0 0 0 0
Nucleus 2.1 -0.9 -2.9 -3.1 – -6.0 -6.2 -6.0 -6.4 -2.1 -5.6 -5.2 -3.7 -1.9 -3.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.2 -1.1 0.4 1.8 – 6.9 7.1 5.1 7.1 2.2 1.2 -0.7 -0.3 -2.2 -0.9
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2.6 – 0 1.9 0 0 0 0 2.0 0 0 0
Vacuole 0 2.2 2.4 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9677 3.2554 2.9744 2.7698 2.752 2.7359 5.8667 5.3278 4.5961 4.6872 4.4467 5.0203 7.7486 7.5784 7.3528 7.341 7.4434 7.3303
Actin 0.0219 0.0002 0.0035 0.0004 0.0032 0.0012 0.0394 0 0.0073 0.0137 0.0363 0.005 0 0 0 0 0 0
Bud 0.0002 0 0.0003 0.0002 0.0001 0.0001 0.0011 0 0.0003 0.0002 0.001 0.0001 0 0 0 0 0.0001 0
Bud Neck 0.0007 0.0001 0.0002 0.0002 0.0007 0.001 0.0034 0.0001 0.0003 0.0005 0.0016 0.0008 0.0001 0.0001 0.0001 0.0001 0.0004 0.0006
Bud Periphery 0.0004 0 0.0002 0.0008 0.0002 0.0001 0.0019 0 0.0007 0.0002 0.0021 0.0002 0 0 0 0 0.0001 0
Bud Site 0.0005 0.0001 0.0008 0.0004 0.0006 0.0001 0.0038 0 0.001 0.0012 0.0031 0.0002 0 0 0 0 0.0002 0
Cell Periphery 0.0001 0 0.0001 0.0001 0.0001 0 0.0005 0 0.0003 0.0001 0.0003 0.0001 0 0 0 0 0 0
Cytoplasm 0.0011 0.0001 0.0042 0.0002 0.0002 0.0002 0.0175 0 0.0029 0.0008 0.0021 0.0003 0 0 0 0 0.0001 0
Cytoplasmic Foci 0.0021 0.0005 0.0019 0.0007 0.0093 0.0001 0.0075 0 0.0037 0.0102 0.0104 0.0006 0 0 0 0 0 0
Eisosomes 0.0003 0 0 0 0 0 0.0005 0 0.0002 0.0001 0.0005 0.0002 0 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0002 0.0004 0.0006 0 0.0004 0.0075 0 0.0029 0.0002 0.0022 0.001 0 0 0 0 0 0
Endosome 0.0098 0.0006 0.0011 0.0024 0.0031 0.0017 0.0216 0 0.0102 0.0027 0.0186 0.0043 0 0 0 0 0 0
Golgi 0.0033 0.0002 0.0005 0.0006 0.0066 0.0002 0.0067 0 0.0023 0.0012 0.0153 0.0018 0 0 0 0 0 0
Lipid Particles 0.0055 0.0038 0.0007 0.0051 0.0034 0.0001 0.0078 0 0.0082 0.0034 0.0487 0.0021 0 0 0 0 0 0
Mitochondria 0.0031 0.0008 0.0068 0.005 0.0014 0.0005 0.0055 0.0001 0.0125 0.0009 0.0137 0.003 0.0003 0 0.0001 0.0001 0.0002 0.0001
None 0.0024 0.0001 0.0009 0.0002 0.0001 0.0001 0.0197 0 0.0019 0.0019 0.0015 0.0004 0 0 0 0 0 0
Nuclear Periphery 0.0091 0.0052 0.0059 0.0073 0.0039 0.0018 0.044 0.0003 0.0121 0.0041 0.0153 0.0109 0.0007 0.0003 0.0009 0.0004 0.0022 0.0001
Nucleolus 0.03 0.0266 0.0206 0.0267 0.1527 0.0908 0.0227 0.0189 0.0198 0.0247 0.1174 0.0564 0.0263 0.0467 0.0264 0.0183 0.1311 0.0737
Nucleus 0.8926 0.9515 0.9473 0.9426 0.781 0.8995 0.7605 0.9801 0.9018 0.9111 0.6594 0.894 0.9717 0.9516 0.9715 0.9808 0.8552 0.9212
Peroxisomes 0.0069 0.0002 0.0003 0.0003 0.013 0 0.003 0 0.0021 0.0091 0.0173 0.0003 0 0 0 0 0 0
Punctate Nuclear 0.0053 0.0087 0.0034 0.0028 0.02 0.0018 0.0187 0.0005 0.0071 0.0128 0.0292 0.0179 0.0007 0.0012 0.0008 0.0004 0.0102 0.004
Vacuole 0.0017 0.0005 0.0006 0.002 0.0003 0.0002 0.0046 0 0.0016 0.0006 0.0021 0.0003 0 0 0 0 0.0001 0
Vacuole Periphery 0.0012 0.0003 0.0005 0.0015 0.0002 0.0001 0.0022 0 0.0009 0.0004 0.0019 0.0004 0 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.37 14.744 10.9407 17.3368 24.592 18.1326 9.69 16.6018 18.3824 13.9422
Translational Efficiency 2.2456 1.8285 1.9941 1.2275 1.243 1.4693 2.4028 1.5536 1.214 1.8468

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
104 641 332 1948 1901 2263 1437 1909 2005 2904 1769 3857

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 846.57 921.87 1043.04 813.41 878.44 952.58 1070.27 1081.10 876.79 945.80 1065.16 945.90
Standard Deviation 154.62 216.77 138.64 156.63 138.84 151.48 157.32 171.63 139.89 168.57 154.35 211.85
Intensity Change Log 2 — 0.122934 0.301093 -0.057647 — 0.116897 0.284959 0.299484 — 0.119863 0.292900 0.135220

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003063 0.001229 0.001446 0.000983 0.000335 0.000587 0.000584 0.000932 0.000476 0.000729 0.000746 0.000958
Bud Neck 0.007531 0.013400 0.015569 0.016176 0.009529 0.017058 0.039355 0.046416 0.009426 0.016251 0.034891 0.031143
Bud Site 0.003248 0.002358 0.001892 0.004153 0.000619 0.001692 0.001315 0.003321 0.000755 0.001839 0.001423 0.003741
Cell Periphery 0.000146 0.000171 0.000052 0.000662 0.000089 0.000106 0.000032 0.000084 0.000092 0.000120 0.000035 0.000376
Cytoplasm 0.001173 0.005745 0.000643 0.016125 0.001038 0.001408 0.000206 0.002356 0.001045 0.002365 0.000288 0.009310
Cytoplasmic Foci 0.002449 0.002071 0.000059 0.001833 0.000051 0.000257 0.000016 0.000676 0.000176 0.000657 0.000024 0.001260
Eisosomes 0.000062 0.000047 0.000063 0.000069 0.000029 0.000043 0.000037 0.000032 0.000031 0.000044 0.000042 0.000051
Endoplasmic Reticulum 0.007521 0.003715 0.003434 0.003631 0.002042 0.002878 0.001537 0.002720 0.002327 0.003063 0.001893 0.003180
Endosome 0.001729 0.001624 0.000154 0.001253 0.000205 0.000587 0.000216 0.000642 0.000284 0.000816 0.000204 0.000951
Golgi 0.001201 0.000492 0.000193 0.000652 0.000093 0.000207 0.000143 0.001604 0.000150 0.000269 0.000152 0.001123
Lipid Particles 0.008360 0.001072 0.000098 0.000214 0.000060 0.000374 0.000014 0.000063 0.000491 0.000528 0.000030 0.000139
Mitochondria 0.005465 0.001473 0.002038 0.002129 0.000979 0.001762 0.002481 0.003235 0.001211 0.001698 0.002398 0.002677
Mitotic Spindle 0.005486 0.003147 0.001654 0.006245 0.003492 0.003037 0.003985 0.014774 0.003595 0.003061 0.003547 0.010466
None 0.003392 0.010234 0.001835 0.052422 0.001955 0.003803 0.001015 0.003011 0.002030 0.005222 0.001169 0.027966
Nuclear Periphery 0.001000 0.000202 0.000238 0.001074 0.000252 0.000700 0.000659 0.001879 0.000291 0.000590 0.000580 0.001472
Nuclear Periphery Foci 0.005175 0.002049 0.000175 0.001053 0.000285 0.000658 0.000101 0.000940 0.000538 0.000965 0.000115 0.000997
Nucleolus 0.100942 0.124934 0.090392 0.050758 0.087151 0.112112 0.064222 0.046529 0.087867 0.114942 0.069133 0.048665
Nucleus 0.823096* 0.822020* 0.876512* 0.835858* 0.891186* 0.850096* 0.883080* 0.866913* 0.887654* 0.843899* 0.881847* 0.851229*
Peroxisomes 0.003520 0.002348 0.001965 0.000520 0.000261 0.000655 0.000278 0.000368 0.000430 0.001028 0.000595 0.000445
Vacuole 0.005468 0.001245 0.001357 0.002988 0.000150 0.001205 0.000459 0.001951 0.000426 0.001214 0.000628 0.002474
Vacuole Periphery 0.009973 0.000425 0.000230 0.001204 0.000198 0.000775 0.000265 0.001554 0.000705 0.000698 0.000259 0.001377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.00 0.75 1.11 1.62 6.53 -6.99 -11.83 -19.82 -12.75 -11.52 -2.42 -3.96 -6.33 -9.16 -6.54
Bud Neck -2.96 -4.93 -5.57 -3.59 -0.19 -9.11 -19.73 -25.07 -19.93 -3.68 -9.44 -20.13 -24.46 -17.02 2.17
Bud Site 0.47 0.43 -0.36 -2.47 -2.90 -5.94 -6.97 -9.98 -6.01 -6.22 -5.17 -6.21 -10.67 -6.50 -6.98
Cell Periphery -0.27 1.43 -4.46 -4.98 -7.32 -0.76 2.83 0.15 1.46 -10.98 -1.29 2.80 -6.05 -5.58 -8.35
Cytoplasm -2.09 0.86 -8.73 -3.19 -9.37 -0.69 2.33 -3.47 -2.30 -9.28 -1.97 2.18 -9.09 -6.40 -11.06
Cytoplasmic Foci 0.21 2.01 0.72 0.54 -4.16 -1.89 1.81 -1.47 -0.20 -1.65 -2.10 2.33 -3.61 -0.82 -4.51
Eisosomes 1.46 -0.81 -0.13 -0.97 0.31 -7.79 -5.72 -5.12 4.04 1.28 -7.75 -8.09 -2.54 -0.81 -0.84
Endoplasmic Reticulum 1.61 1.55 1.65 0.15 0.58 -3.98 2.86 -8.16 -1.44 -12.38 -3.52 1.04 -6.64 -2.04 -10.89
Endosome 0.09 2.00 0.79 0.55 -4.15 -2.85 0.05 -3.22 0.52 -2.69 -2.29 0.97 -4.05 0.04 -4.58
Golgi 1.27 1.83 1.15 -0.15 -1.58 -3.04 -3.61 -1.81 -1.55 -1.64 -2.77 -1.09 -2.38 -1.84 -2.25
Lipid Particles 1.35 1.57 1.56 1.37 -2.24 -1.36 2.22 -2.06 1.16 -5.29 -0.21 1.63 1.20 1.65 -7.26
Mitochondria 2.67 2.22 2.17 -2.61 -0.28 -2.79 -3.10 -9.17 -6.21 -1.84 -1.94 -2.89 -6.46 -5.09 -0.84
Mitotic Spindle 0.47 0.57 -0.67 -2.50 -3.23 0.13 -1.22 -6.17 -6.65 -4.84 0.20 -1.01 -6.05 -7.23 -4.66
None -2.76 0.93 -11.61 -8.45 -12.70 -2.96 3.00 -5.51 0.00 -11.18 -4.54 2.56 -12.92 -10.37 -13.42
Nuclear Periphery 1.23 1.07 -0.14 -2.15 -1.89 -2.65 -2.17 -7.65 -4.63 -4.07 -2.20 -1.98 -5.20 -3.53 -3.03
Nuclear Periphery Foci 0.93 1.86 1.57 2.19 -4.79 -1.68 1.68 -2.34 -0.27 -3.63 -1.97 2.40 -1.62 0.90 -6.19
Nucleolus -1.99 0.23 3.87 12.75 5.77 -6.32 3.40 7.59 13.68 3.48 -7.49 2.63 9.95 19.01 6.24
Nucleus 0.17 -1.67 -0.66 -2.17 2.66 8.95 5.11 9.72 1.85 4.22 10.05 4.84 10.64 0.89 5.13
Peroxisomes 0.84 1.35 2.98 3.37 6.66 -2.92 -2.08 -5.35 1.53 -3.95 -3.64 -3.04 -1.01 3.45 3.12
Vacuole 1.44 1.22 0.81 -3.01 -2.01 -3.47 -2.54 -5.36 -2.49 -3.95 -2.70 -1.98 -6.90 -3.97 -5.47
Vacuole Periphery 1.32 1.34 1.23 -1.94 -2.54 -3.39 -1.56 -2.33 -0.47 -2.06 0.01 1.13 -0.66 -1.14 -3.19
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Med7

Med7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Med7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available