Standard name
Human Ortholog
Description 6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.86 0.91 0.85 0.87 0.83 0.75 0.53 0.55 0.42 0.46 0.34 0.43 0.87 0.91 0.88 – 0.5 – 0.67 0.67 0.59 0.78 0.82 0.76
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.1 0 0 – 0.06 – 0 0 0 0 0 0
Endosome 0 0 0.09 0 0.08 0 0 0 0 0 0 0 0 0 0.07 – 0.12 – 0.08 0.07 0.15 0.09 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0.09 0.3 0.53 0.42 0.63 0.65 0.77 0.65 0 0 0 – 0 – 0.1 0.11 0.1 0 0 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.07 0.05 0.06 0.13 0.19 0.13 0.14 0.13 0.18 0 0 0 – 0.38 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 1 0 0 1 0 0 1 0 0 3 3 2 – 0 – 9 1 6 0 3 6
Bud 6 1 4 1 2 6 21 10 24 20 18 18 0 0 1 – 0 – 2 0 1 0 2 5
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 2 – 0 – 1 4 2 1 2 3
Bud Site 0 0 0 0 0 0 0 2 1 3 1 5 0 0 0 – 0 – – – – – – –
Cell Periphery 1 0 0 0 2 3 1 1 0 3 2 1 1 3 3 – 0 – 0 0 0 0 2 0
Cytoplasm 251 61 171 267 360 345 397 374 234 295 152 237 181 284 343 – 8 – 222 73 144 81 236 265
Endoplasmic Reticulum 11 4 5 7 2 3 5 2 0 3 2 1 20 11 11 – 1 – 8 1 5 0 1 3
Endosome 3 0 17 10 36 17 21 29 3 2 1 1 5 12 28 – 2 – 26 7 36 9 10 17
Golgi 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 – 0 – 0 0 2 0 0 0
Mitochondria 26 3 4 13 41 136 393 282 351 418 346 358 0 0 2 – 0 – 33 11 24 2 9 24
Nucleus 0 1 0 0 0 2 11 12 13 14 2 13 1 1 0 – 0 – 0 0 0 2 1 1
Nuclear Periphery 5 0 0 0 0 2 10 2 5 0 0 5 0 0 0 – 0 – 1 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 – 0 0 0 0 0 0
SpindlePole 1 0 1 1 2 0 2 4 5 1 0 0 2 1 4 – 1 – 12 2 7 1 7 8
Vac/Vac Membrane 7 0 12 23 22 29 98 125 71 91 59 98 1 8 16 – 6 – 8 4 3 1 3 6
Unique Cell Count 292 67 200 307 432 460 746 675 555 647 452 547 208 312 392 16 332 109 245 105 288 351
Labelled Cell Count 312 70 216 324 467 543 960 845 709 851 583 739 214 323 414 18 332 109 245 105 288 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.7 5.3 6.3 5.4 4.6 4.3 4.4 4.0 3.9 3.7 3.7 7.3 7.1 6.4 – 8.1 – 7.0 7.1 6.9
Std Deviation (1e-4) 0.6 0.7 1.3 1.7 1.6 1.1 1.1 1.2 1.1 1.1 1.2 1.0 1.3 1.2 1.2 – 2.0 – 1.5 1.5 1.4
Intensity Change (Log2) – – – 0.24 0.03 -0.21 -0.31 -0.28 -0.42 -0.44 -0.52 -0.52 0.46 0.42 0.26 – 0.61 – 0.39 0.41 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3379 3.8631 3.7046 3.4984 3.0217 3.461 -0.2972 1.0725 1.0702 1.1865 0.2656 0.7568 -0.5437 1.0544 0.3708 0.258 0.1714 0.002
Actin 0.0194 0.0012 0.0224 0.0097 0.0195 0.0027 0.0219 0.0084 0.0028 0.0569 0.003 0.003 0.0003 0.0005 0.001 0.0009 0.0005 0.0005
Bud 0.0009 0.0018 0.0009 0.0004 0.0005 0.0003 0.0004 0.0029 0.0006 0.0003 0.0024 0.0101 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.012 0.0007 0.0016 0.0018 0.0017 0.0017 0.011 0.0004 0.0004 0.0005 0.0005 0.0029 0.0003 0.0003 0.0008 0.002 0.0007 0.0011
Bud Periphery 0.0015 0.001 0.001 0.0004 0.0007 0.0004 0.0004 0.0016 0.0004 0.0001 0.0031 0.0106 0 0.0001 0.0001 0 0 0
Bud Site 0.0068 0.0122 0.0048 0.0062 0.0014 0.0005 0.0043 0.0111 0.0034 0.0005 0.0033 0.0149 0.0003 0.0031 0.0007 0.0003 0.0002 0.0001
Cell Periphery 0.0005 0.0004 0.0002 0.0002 0.0001 0.0001 0.0004 0.0003 0.0002 0 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.1957 0.34 0.3113 0.2184 0.2523 0.308 0.2963 0.5742 0.4798 0.518 0.2499 0.4236 0.3192 0.4858 0.3875 0.5244 0.4565 0.4701
Cytoplasmic Foci 0.1981 0.1981 0.2268 0.2193 0.209 0.2335 0.2154 0.1309 0.1543 0.1554 0.2114 0.2083 0.1847 0.1603 0.1973 0.1233 0.2082 0.1717
Eisosomes 0.0005 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0174 0.0131 0.013 0.0111 0.011 0.011 0.0237 0.0135 0.024 0.0061 0.0087 0.0169 0.0244 0.0122 0.0181 0.0106 0.0135 0.0183
Endosome 0.2622 0.2166 0.2041 0.3065 0.2567 0.2112 0.2214 0.1411 0.2087 0.215 0.3027 0.2358 0.3074 0.2492 0.2562 0.227 0.2058 0.2427
Golgi 0.0174 0.0094 0.0106 0.0149 0.0173 0.0104 0.0164 0.005 0.0089 0.0088 0.025 0.0157 0.0075 0.0069 0.0085 0.005 0.008 0.0063
Lipid Particles 0.0481 0.0239 0.0157 0.0382 0.0123 0.0237 0.0256 0.0052 0.0102 0.0037 0.0375 0.0122 0.0158 0.0045 0.01 0.0116 0.0058 0.0066
Mitochondria 0.0122 0.0014 0.0024 0.0025 0.0047 0.0051 0.003 0.0005 0.0161 0.0011 0.0515 0.0114 0.0005 0.0007 0.001 0.0014 0.0012 0.0011
None 0.1193 0.1246 0.1393 0.1172 0.1435 0.1432 0.0968 0.0811 0.0559 0.0044 0.0105 0.0058 0.0937 0.05 0.0791 0.0496 0.0663 0.0375
Nuclear Periphery 0.0164 0.0064 0.0091 0.0072 0.0038 0.0082 0.0067 0.0064 0.0088 0.0041 0.0134 0.006 0.0129 0.006 0.0079 0.0116 0.0099 0.0122
Nucleolus 0.0044 0.0024 0.0007 0.001 0.0009 0.0021 0.0022 0.0002 0.0004 0 0.0004 0.0002 0.0002 0.0001 0.0006 0.0003 0.0001 0.0001
Nucleus 0.0158 0.012 0.0058 0.0048 0.0031 0.0063 0.0036 0.0035 0.0059 0.0017 0.0025 0.0034 0.0037 0.0038 0.0044 0.0104 0.005 0.0062
Peroxisomes 0.0186 0.0097 0.0096 0.027 0.0236 0.0128 0.0287 0.0034 0.0031 0.0031 0.0321 0.0079 0.004 0.0024 0.0087 0.002 0.0048 0.0063
Punctate Nuclear 0.0201 0.0151 0.012 0.0079 0.0319 0.0098 0.0072 0.0029 0.0042 0.0072 0.0045 0.0044 0.0093 0.0037 0.008 0.0069 0.0065 0.0081
Vacuole 0.0103 0.0088 0.0071 0.0038 0.0044 0.0076 0.0131 0.0068 0.0098 0.0101 0.026 0.0042 0.0136 0.0085 0.0085 0.01 0.0057 0.0085
Vacuole Periphery 0.0023 0.0011 0.0014 0.0013 0.0014 0.0014 0.0015 0.0007 0.002 0.0026 0.0112 0.0022 0.0019 0.0013 0.0016 0.0023 0.0013 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.6479 18.1744 12.2602 27.9792 13.7789 18.4723 33.8288 26.4966 21.9236 24.9094
Translational Efficiency 0.4919 0.6922 0.8706 0.3393 0.5956 1.0017 0.4656 0.5516 0.7603 0.5913

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
165 153 654 350 133 1359 370 815 298 1512 1024 1165

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.49 696.98 816.30 1011.50 747.91 734.86 979.48 994.89 706.15 731.03 875.26 999.88
Standard Deviation 86.29 111.95 107.03 134.90 93.97 81.23 118.47 130.32 97.31 85.62 136.13 131.93
Intensity Change Log 2 — 0.051604 0.279587 0.588912 — -0.025395 0.389151 0.411672 — 0.011573 0.338313 0.498305

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000665 0.000416 0.008057 0.006663 0.000667 0.000441 0.013292 0.004498 0.000666 0.000439 0.009949 0.005149
Bud Neck 0.009111 0.014452 0.013258 0.008906 0.003993 0.021122 0.017125 0.008635 0.006827 0.020447 0.014655 0.008716
Bud Site 0.003746 0.011568 0.034671 0.046679 0.010128 0.012154 0.032824 0.050561 0.006594 0.012094 0.034004 0.049395
Cell Periphery 0.000148 0.000148 0.000198 0.000230 0.000314 0.000120 0.000281 0.000409 0.000222 0.000123 0.000228 0.000355
Cytoplasm 0.237766* 0.302754* 0.289634* 0.230399* 0.460822* 0.351871* 0.149932* 0.305737* 0.337318* 0.346901* 0.239156* 0.283103*
Cytoplasmic Foci 0.438202* 0.309333* 0.146470 0.057798 0.273958* 0.340121* 0.093991 0.061463 0.364899* 0.337005* 0.127508 0.060362
Eisosomes 0.000319 0.000164 0.000096 0.000069 0.000143 0.000142 0.000137 0.000059 0.000241 0.000144 0.000111 0.000062
Endoplasmic Reticulum 0.002402 0.004084 0.008129 0.004079 0.008482 0.001811 0.011709 0.004915 0.005116 0.002041 0.009422 0.004664
Endosome 0.061261 0.073015 0.042411 0.028896 0.028863 0.047168 0.040679 0.020305 0.046801 0.049783 0.041785 0.022886
Golgi 0.006529 0.006785 0.003784 0.007953 0.002473 0.006974 0.001987 0.008162 0.004719 0.006955 0.003135 0.008099
Lipid Particles 0.039165 0.021930 0.023940 0.011986 0.017823 0.017244 0.032915 0.004621 0.029640 0.017718 0.027183 0.006834
Mitochondria 0.009222 0.005924 0.001653 0.000629 0.001251 0.010900 0.000852 0.006160 0.005664 0.010397 0.001363 0.004498
Mitotic Spindle 0.005681 0.007706 0.143890* 0.365369* 0.000584 0.010202 0.231223* 0.283859* 0.003406 0.009949 0.175446* 0.308347*
None 0.001109 0.011949 0.001802 0.002272 0.003430 0.001766 0.002075 0.001734 0.002145 0.002796 0.001901 0.001896
Nuclear Periphery 0.000776 0.001091 0.005517 0.002137 0.000739 0.000657 0.003646 0.001914 0.000759 0.000700 0.004841 0.001981
Nuclear Periphery Foci 0.003006 0.004908 0.033766 0.023054 0.010941 0.001127 0.069007 0.011207 0.006547 0.001509 0.046499 0.014766
Nucleolus 0.002146 0.005201 0.002806 0.000635 0.002480 0.001882 0.001109 0.000747 0.002295 0.002218 0.002192 0.000713
Nucleus 0.075805 0.079538 0.081949 0.041866 0.099561 0.064634 0.074409 0.047322 0.086408 0.066142 0.079225 0.045683
Peroxisomes 0.019527 0.011608 0.026956 0.007048 0.005718 0.014259 0.034890 0.005490 0.013364 0.013991 0.029823 0.005958
Vacuole 0.081189 0.124297 0.130157 0.152498 0.066168 0.092718 0.187517 0.165504 0.074485 0.095913 0.150883 0.161597
Vacuole Periphery 0.002224 0.003130 0.000858 0.000833 0.001461 0.002689 0.000400 0.006700 0.001884 0.002734 0.000693 0.004938

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.83 -4.51 -2.48 -3.03 2.52 1.07 -3.45 -2.17 -3.31 2.77 1.31 -5.67 -3.25 -4.44 4.07
Bud Neck -2.14 -3.56 -1.68 1.02 1.81 -9.79 -4.32 -4.51 5.63 1.84 -8.68 -5.93 -3.53 5.86 2.88
Bud Site -2.22 -8.82 -6.83 -3.42 -0.23 -0.48 -3.26 -3.64 -5.54 -0.48 -2.46 -7.83 -7.70 -6.86 -0.07
Cell Periphery -0.21 -2.88 -2.56 -2.59 -0.09 2.94 0.96 1.52 -1.28 1.11 3.09 0.05 0.59 -1.86 0.71
Cytoplasm -2.57 -2.26 1.91 4.57 5.27 3.75 10.44 6.49 6.26 -7.82 -0.92 5.20 4.49 9.12 -1.18
Cytoplasmic Foci 5.31 16.56 21.78 14.53 11.40 -3.42 10.21 12.04 38.98 4.03 2.00 17.73 22.94 42.50 12.01
Eisosomes 2.74 4.19 4.94 5.11 4.66 0.46 1.42 6.61 13.23 6.52 3.26 4.44 6.37 13.95 9.04
Endoplasmic Reticulum -0.81 -8.42 -2.65 -0.01 7.00 2.48 -1.31 1.98 -2.85 8.65 2.42 -3.69 1.34 -3.32 11.13
Endosome -0.79 3.09 5.49 5.76 6.04 -3.37 -0.70 3.97 14.30 7.55 -0.28 2.41 6.77 15.05 10.22
Golgi -0.07 1.53 1.50 1.40 0.22 -5.38 2.10 0.29 5.23 -1.46 -1.53 1.82 1.59 5.48 -0.26
Lipid Particles 2.84 3.10 5.28 3.21 4.68 0.23 -1.79 3.94 8.62 7.89 3.47 1.80 6.92 8.01 10.06
Mitochondria 0.49 1.20 1.40 2.78 6.65 -5.93 0.71 -0.64 5.57 -1.61 -1.36 1.24 1.19 6.28 -0.27
Mitotic Spindle -0.31 -11.64 -13.09 -12.72 -6.65 -5.50 -12.04 -19.50 -18.52 -1.97 -2.39 -16.78 -23.03 -22.57 -7.19
None -1.65 -2.14 -2.55 1.46 -1.20 1.41 1.22 0.92 -1.30 -0.87 -0.71 0.56 -0.43 0.53 -1.98
Nuclear Periphery -0.75 -6.39 -6.85 -5.14 3.19 0.53 -9.71 -7.68 -9.02 4.98 0.60 -8.86 -9.86 -11.45 5.03
Nuclear Periphery Foci -1.04 -9.02 -7.22 -5.35 1.51 3.39 -7.23 -1.17 -8.64 7.12 3.68 -10.76 -5.81 -11.26 7.26
Nucleolus -1.61 -0.27 1.73 2.45 3.70 1.07 0.85 0.92 -0.05 0.20 0.42 0.22 1.37 1.30 1.64
Nucleus -0.27 -1.38 2.08 2.52 5.48 2.89 0.93 2.32 -1.28 2.01 2.38 -0.38 2.47 0.28 4.46
Peroxisomes 2.31 -0.30 5.07 4.57 7.72 -5.35 -3.21 1.91 9.74 4.00 -0.16 -2.43 5.36 10.56 8.50
Vacuole -2.54 -8.14 -12.54 -9.26 -7.54 -2.22 -11.43 -14.63 -16.95 -0.98 -2.57 -13.55 -19.33 -20.55 -7.05
Vacuole Periphery -0.80 1.76 2.15 3.66 2.23 -3.14 3.41 -0.31 1.54 -2.08 -1.65 2.61 0.88 2.96 -1.59
Standard name
Human Ortholog
Description 6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pfk27

Pfk27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pfk27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available