Standard name
Human Ortholog
Description Phosphotyrosine-specific phosphatase; major role in osmolarity sensing through dephosphorylation of the Hog1p MAPK with a minor role by Ptp3p; inactivates and regulates Hog1p localization; major role in the cell wall integrity pathway through dephosphorylation of MAPK Slt2p with a minor role by Ptp3p; minor role with Msg5p in the pheromone adaptive response through dephosphorylation of MAPK Fus3p with major role by Ptp3p; co-regulates the calcium signaling pathway with Msg5p; nuclear localized

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.68 0.87 0.8 0.52 0.42 0.35 0.44 0.26 0.28 0.29 0.43 0.72 0.77 0.76 0.43 0.41 0.33 0.37 0.52 0.49 0.47 0.48 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.3 0 0.14 0.08 0.29 0.37 0.27 0.73 0.66 0.67 0.49 0.07 0 0.07 0 0 0 0.06 0 0.07 0.08 0.06 0
Nucleus 0.08 0.06 0 0.07 0.26 0.36 0.37 0.32 0.29 0.2 0.23 0.19 0 0 0 0.18 0.18 0.15 0.24 0.16 0.12 0.07 0 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0.08 0.07 0 0.16 0.13 0.07 0.06 0 0.06 0.05 0.05 0.28 0.29 0.24 0.39 0.46 0.6 0.26 0.21 0.24 0.28 0.27 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 6 3
Bud 1 0 0 0 0 1 3 5 6 11 8 7 0 0 0 0 0 1 0 0 1 0 10 12
Bud Neck 0 0 6 3 3 3 8 11 0 2 5 10 0 0 0 0 0 0 0 1 1 0 6 12
Bud Site 0 0 1 0 0 0 1 5 4 7 5 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 1 0 1 1 0 1 0 1 1 0 0 0 0 0 0 0 1 0
Cytoplasm 62 96 156 117 97 119 132 136 62 76 92 124 195 302 289 51 49 13 48 94 127 70 181 181
Endoplasmic Reticulum 1 1 0 0 0 0 2 0 0 2 2 1 0 3 1 4 2 2 0 0 2 2 6 2
Endosome 0 0 1 0 1 3 0 2 0 0 0 0 3 3 0 3 6 1 2 0 2 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 2
Mitochondria 25 43 2 21 14 82 140 84 172 179 215 143 20 14 25 4 3 1 7 6 19 11 21 13
Nucleus 8 9 7 11 48 101 140 97 67 53 73 54 5 4 10 21 22 6 31 28 30 10 13 18
Nuclear Periphery 2 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 2 2 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 1 1 1 3 2 8 1 2 0 3 0 0 0 0 0 0 1 3 4 1 9 12
Vac/Vac Membrane 28 11 13 3 30 36 27 19 8 17 15 14 77 115 91 47 55 24 33 38 62 42 102 74
Unique Cell Count 101 142 179 147 185 282 375 307 235 270 322 291 272 393 379 119 120 40 129 184 262 150 377 345
Labelled Cell Count 127 160 187 157 195 348 458 367 321 351 417 362 300 442 417 133 140 48 129 184 262 150 377 345


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.3 4.6 4.2 4.9 3.9 4.0 4.2 3.3 3.4 3.2 3.7 4.1 4.3 4.5 6.4 6.6 6.4 4.3 4.4 4.8
Std Deviation (1e-4) 0.6 0.4 0.9 1.1 1.2 0.9 0.9 1.1 0.7 1.2 0.8 1.0 0.7 0.9 1.3 1.2 1.4 1.5 1.7 1.6 1.5
Intensity Change (Log2) -0.13 0.11 -0.22 -0.2 -0.13 -0.48 -0.44 -0.49 -0.31 -0.15 -0.1 -0.03 0.48 0.52 0.49 -0.1 -0.07 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 6.8 7.8 8.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1048 -0.8347 -1.0143 -0.5707 -0.8904 -0.6386 -0.1709 -0.4429 -0.6081 -0.5591 -1.0211 -0.6589 -1.0285 -0.7196 -1.1832 -0.9538 -1.3975 -0.9538
Actin 0.1354 0.0023 0.0184 0.0056 0.0018 0.0006 0.0342 0.0002 0.0029 0.0051 0.0017 0.001 0.0181 0.0002 0.0109 0.0003 0.0754 0.0009
Bud 0.0024 0.0026 0.0003 0.0002 0.0002 0.0002 0.0012 0.002 0.0012 0.0002 0.0097 0.0002 0.0004 0.0078 0.0012 0.0001 0.0011 0.0001
Bud Neck 0.002 0.0002 0.0008 0.0003 0.0011 0.0014 0.0054 0.0003 0.0008 0.0004 0.0013 0.0028 0.0003 0.0002 0.0004 0.0003 0.0024 0.0015
Bud Periphery 0.0026 0.0044 0.0002 0.0002 0.0001 0.0004 0.0025 0.0013 0.0029 0.0004 0.0112 0.0003 0.0007 0.0005 0.0003 0 0.0032 0.0001
Bud Site 0.0094 0.0031 0.0014 0.0004 0.0062 0.0002 0.0109 0.0048 0.0118 0.0003 0.0054 0.0002 0.0009 0.0058 0.0027 0.0001 0.001 0.0001
Cell Periphery 0.0004 0.0002 0.0001 0.0001 0.0001 0.0004 0.0007 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0
Cytoplasm 0.0869 0.1374 0.2955 0.0713 0.0867 0.2512 0.2189 0.2925 0.2022 0.1225 0.2704 0.3081 0.3363 0.3577 0.2808 0.2051 0.2881 0.3679
Cytoplasmic Foci 0.0795 0.0071 0.012 0.0139 0.1504 0.0048 0.0375 0.0119 0.0179 0.0052 0.0321 0.0143 0.0231 0.0054 0.0147 0.0206 0.0189 0.0243
Eisosomes 0.0009 0.0004 0.0002 0.0001 0.0001 0.0002 0.0005 0 0.0001 0.0002 0 0 0.0001 0.0001 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.0194 0.0049 0.0048 0.0024 0.0013 0.0133 0.0073 0.0054 0.0039 0.0014 0.008 0.003 0.0035 0.0041 0.004 0.0015 0.0032 0.0031
Endosome 0.0632 0.0016 0.0023 0.0386 0.0144 0.0555 0.0386 0.0053 0.0127 0.0132 0.0162 0.0137 0.0138 0.009 0.006 0.0296 0.0075 0.007
Golgi 0.0404 0.0002 0.0009 0.0442 0.0054 0.0038 0.0145 0.0003 0.0064 0.0209 0.0095 0.0052 0.0133 0.0012 0.0066 0.0398 0.0054 0.0086
Lipid Particles 0.0312 0.0014 0.0034 0.0268 0.0207 0.0053 0.0311 0.0008 0.0069 0.0058 0.0366 0.0024 0.025 0.0004 0.0046 0.0019 0.0019 0.0036
Mitochondria 0.0468 0.0014 0.0019 0.008 0.0004 0.003 0.0142 0.0005 0.0094 0.0459 0.0098 0.0103 0.0124 0.0005 0.0038 0.0065 0.0046 0.004
None 0.371 0.7544 0.539 0.702 0.545 0.5437 0.3661 0.4977 0.4594 0.5527 0.2931 0.3119 0.3899 0.474 0.4783 0.4553 0.2668 0.3941
Nuclear Periphery 0.0198 0.005 0.0096 0.0037 0.0035 0.0239 0.0307 0.0087 0.0106 0.0067 0.013 0.0105 0.007 0.008 0.0081 0.0042 0.0159 0.006
Nucleolus 0.0011 0.0011 0.0021 0.0006 0.0018 0.0046 0.0058 0.0013 0.0026 0.0005 0.0046 0.0022 0.0005 0.0007 0.0026 0.0005 0.0061 0.0008
Nucleus 0.0352 0.0599 0.0889 0.0714 0.0101 0.0564 0.1267 0.1503 0.2154 0.2075 0.193 0.2742 0.1213 0.1165 0.1554 0.2172 0.2717 0.1542
Peroxisomes 0.0319 0.0006 0.0018 0.0035 0.0068 0.0002 0.0296 0.0054 0.0163 0.0018 0.0226 0.0076 0.0267 0.0002 0.0053 0.0007 0.0024 0.0022
Punctate Nuclear 0.0135 0.0097 0.0148 0.0044 0.1433 0.0107 0.0106 0.0083 0.0129 0.0077 0.0579 0.0286 0.0045 0.0057 0.0121 0.0144 0.0173 0.0199
Vacuole 0.0043 0.0016 0.0011 0.0014 0.0006 0.0126 0.0078 0.0025 0.0028 0.0008 0.0028 0.0023 0.0018 0.0017 0.0017 0.0011 0.005 0.0012
Vacuole Periphery 0.0026 0.0004 0.0003 0.0008 0.0001 0.0078 0.0053 0.0003 0.0006 0.0006 0.0009 0.0011 0.0005 0.0002 0.0003 0.0006 0.0021 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1822 12.2586 7.7969 11.2639 10.4772 13.2159 14.9399 12.5483 10.215 16.8983
Translational Efficiency 0.2896 0.3888 0.3667 0.2614 0.2445 0.4194 0.362 0.3478 0.3962 0.2848

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2285 1280 413 1323 319 1450 167 79 2604 2730 580 1402

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.14 717.35 900.49 819.37 670.34 711.95 885.33 878.60 671.92 714.48 896.12 822.71
Standard Deviation 68.52 93.87 99.30 119.82 67.36 110.09 93.43 101.65 68.38 102.84 97.89 119.65
Intensity Change Log 2 0.093915 0.421948 0.285753 0.086883 0.401322 0.390313 0.090408 0.411686 0.338910

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000044 0.000305 0.000732 0.001148 0.000052 0.000271 0.000265 0.000989 0.000045 0.000287 0.000597 0.001139
Bud Neck 0.006203 0.014535 0.003427 0.015693 0.004416 0.013205 0.001532 0.001594 0.005984 0.013829 0.002882 0.014898
Bud Site 0.001781 0.006732 0.003785 0.029199 0.003613 0.006854 0.000932 0.016457 0.002005 0.006797 0.002963 0.028481
Cell Periphery 0.000166 0.000149 0.000116 0.000238 0.000219 0.000187 0.000236 0.000156 0.000172 0.000169 0.000151 0.000234
Cytoplasm 0.385484* 0.340378* 0.058309 0.273597* 0.288139* 0.324414* 0.162551* 0.254377* 0.373559* 0.331899* 0.088323 0.272514*
Cytoplasmic Foci 0.048459 0.115903 0.002078 0.010748 0.115859 0.125528 0.030514 0.028267 0.056716 0.121016 0.010266 0.011736
Eisosomes 0.000049 0.000080 0.000050 0.000043 0.000098 0.000099 0.000129 0.000076 0.000055 0.000090 0.000073 0.000045
Endoplasmic Reticulum 0.000547 0.001097 0.010920 0.003028 0.000681 0.000954 0.004637 0.003779 0.000563 0.001021 0.009111 0.003070
Endosome 0.001268 0.006030 0.001133 0.008555 0.004174 0.007914 0.004842 0.004234 0.001624 0.007031 0.002201 0.008312
Golgi 0.000266 0.002114 0.000068 0.004845 0.000435 0.002539 0.005280 0.000094 0.000287 0.002340 0.001569 0.004577
Lipid Particles 0.003850 0.005637 0.001091 0.000962 0.007916 0.006942 0.003986 0.002251 0.004348 0.006330 0.001925 0.001035
Mitochondria 0.001513 0.007051 0.000662 0.005218 0.005791 0.008103 0.000456 0.000304 0.002037 0.007610 0.000603 0.004941
Mitotic Spindle 0.001172 0.004542 0.000167 0.042684 0.000064 0.006275 0.000066 0.001424 0.001036 0.005462 0.000138 0.040360
None 0.008854 0.010930 0.002603 0.014279 0.004170 0.006291 0.003388 0.004555 0.008280 0.008466 0.002829 0.013732
Nuclear Periphery 0.000766 0.000908 0.001725 0.001521 0.001046 0.001165 0.001495 0.001626 0.000800 0.001044 0.001659 0.001527
Nuclear Periphery Foci 0.000191 0.000901 0.000735 0.000759 0.000303 0.000464 0.000955 0.001808 0.000205 0.000669 0.000798 0.000818
Nucleolus 0.001199 0.004390 0.000882 0.001463 0.002428 0.004438 0.001353 0.000499 0.001349 0.004416 0.001018 0.001408
Nucleus 0.528409* 0.440655* 0.887827* 0.532699* 0.545286* 0.443971* 0.712078* 0.601307* 0.530476* 0.442416* 0.837224* 0.536565*
Peroxisomes 0.001384 0.010877 0.000205 0.002175 0.002113 0.009525 0.000512 0.000698 0.001473 0.010159 0.000293 0.002092
Vacuole 0.008236 0.025821 0.023336 0.046762 0.012872 0.029899 0.064566 0.075366 0.008804 0.027987 0.035207 0.048374
Vacuole Periphery 0.000161 0.000964 0.000149 0.004384 0.000325 0.000960 0.000228 0.000139 0.000181 0.000962 0.000172 0.004144

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.12 -10.50 -5.97 -3.69 0.65 -4.31 -6.77 -1.69 -1.25 -1.01 -5.92 -11.61 -6.20 -4.16 -0.41
Bud Neck -5.29 1.23 -4.52 1.15 -6.11 -5.70 2.73 2.38 8.05 -0.74 -6.69 2.30 -4.61 1.36 -6.86
Bud Site -4.68 -1.40 -8.15 -5.14 -6.11 -1.00 0.72 -0.80 -0.51 -1.04 -4.91 -1.00 -7.87 -5.17 -6.84
Cell Periphery 0.60 1.11 -0.24 -1.77 -2.86 0.74 -0.06 1.43 1.33 2.88 0.17 0.49 0.03 -0.26 -0.91
Cytoplasm 5.60 45.18 20.63 12.39 -19.31 -2.29 7.29 3.53 5.20 -1.80 6.63 38.05 20.26 14.42 -15.42
Cytoplasmic Foci -13.45 23.66 20.27 23.02 -5.72 -1.12 10.12 9.52 14.04 0.71 -17.14 20.30 22.92 34.36 0.52
Eisosomes -6.89 -2.53 -1.10 6.73 1.98 -0.08 -2.80 -0.18 -0.14 2.50 -8.23 -5.56 -0.06 9.07 5.94
Endoplasmic Reticulum -4.62 -14.26 -18.94 -17.42 9.70 -2.40 -7.95 -7.27 -6.95 0.56 -4.44 -15.97 -20.33 -19.39 9.86
Endosome -8.82 -1.35 -4.25 2.24 -3.92 -2.37 -0.04 0.41 3.98 0.55 -10.54 -1.82 -3.76 3.71 -2.57
Golgi -3.21 2.77 -2.47 0.18 -2.78 -2.74 -0.87 2.66 3.27 1.00 -4.22 -0.74 -2.43 0.61 -0.87
Lipid Particles -3.03 6.01 6.59 9.82 1.44 0.57 2.63 3.70 8.00 2.97 -4.02 5.30 7.77 15.43 5.52
Mitochondria -5.93 2.49 -2.42 3.84 -4.21 -0.66 1.54 1.56 7.05 0.74 -6.38 2.74 -1.18 5.36 -4.33
Mitotic Spindle -2.67 -0.50 -8.02 -6.63 -6.92 -4.10 -1.47 -1.11 2.95 -0.97 -4.05 -0.37 -8.07 -6.38 -7.43
None -1.22 6.82 -0.83 0.46 -7.14 -2.58 1.97 0.80 2.78 -0.78 -0.18 6.90 -1.05 -0.96 -7.08
Nuclear Periphery -1.89 -10.71 -15.86 -14.39 -2.45 -0.49 -4.56 -4.28 -4.22 -1.50 -2.45 -12.38 -16.29 -12.75 -3.22
Nuclear Periphery Foci -1.89 -5.27 -5.93 -0.24 0.33 -2.61 -6.07 -4.71 -4.10 -1.60 -2.49 -6.89 -6.74 -1.85 0.55
Nucleolus -5.13 2.17 1.05 5.56 -1.32 -2.87 2.74 3.89 6.48 3.44 -6.81 2.87 2.03 7.83 -0.83
Nucleus 8.66 -33.96 -7.34 -13.62 22.07 5.62 -5.77 -1.98 -5.33 2.18 11.31 -27.61 -7.52 -16.19 17.41
Peroxisomes -7.89 4.39 0.45 7.78 -2.22 -7.17 5.81 4.37 8.91 -0.26 -10.92 5.06 0.76 10.49 -2.09
Vacuole -11.55 -7.42 -18.33 -12.55 -7.70 -7.53 -8.07 -6.54 -5.57 -1.24 -15.88 -10.72 -19.27 -13.51 -4.46
Vacuole Periphery -6.14 0.24 -2.85 -1.47 -2.85 -3.26 1.49 2.43 4.57 1.92 -6.88 0.47 -2.81 -1.39 -2.82
Standard name
Human Ortholog
Description Phosphotyrosine-specific phosphatase; major role in osmolarity sensing through dephosphorylation of the Hog1p MAPK with a minor role by Ptp3p; inactivates and regulates Hog1p localization; major role in the cell wall integrity pathway through dephosphorylation of MAPK Slt2p with a minor role by Ptp3p; minor role with Msg5p in the pheromone adaptive response through dephosphorylation of MAPK Fus3p with major role by Ptp3p; co-regulates the calcium signaling pathway with Msg5p; nuclear localized
Localization
Cell Percentages nucleus (15%), cytoplasm (5%), mixed (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ptp2

Ptp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available