Standard name
Human Ortholog
Description Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.98 0.99 0.97 0.92 0.91 0.9 0.74 0.77 0.7 0.72 0.98 0.98 1.0 0.94 0.94 0.88 0.71 0.96 0.87 0.7 0.74 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.1 0.08 0.35 0.32 0.43 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.14 0 0.05 0.13 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 0 2 6 1 0 0 2 3 1 0 0 0 0 1 0 0 1 0 2 1
Bud 0 0 4 1 2 0 2 0 6 8 8 5 0 2 1 0 0 0 0 0 0 1 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 3 7 5 13 15 19 6 15 11 12 15 7 9 7 18 18 14 0 0 0 0 0 0
Cytoplasm 154 267 215 226 366 320 508 333 264 279 195 255 274 369 552 346 173 138 138 268 198 140 411 350
Endoplasmic Reticulum 2 8 3 2 3 10 13 14 1 1 3 4 3 3 2 12 4 7 7 1 2 18 68 47
Endosome 0 1 0 0 2 0 0 0 2 2 0 6 0 0 0 0 0 1 0 0 1 0 1 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 3 0 0 1 3 3
Mitochondria 3 3 1 3 10 30 56 28 126 116 120 104 2 0 2 3 1 2 4 1 6 5 16 9
Nucleus 6 0 1 4 1 0 3 4 3 0 1 3 2 1 1 6 2 4 3 0 2 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 3 1 1 1 3 5 1 9 10 12 6 8 1 3 0 19 5 8 27 5 10 25 41 28
Unique Cell Count 163 276 219 228 378 348 557 372 356 362 280 353 281 375 553 368 185 157 194 281 227 200 558 450
Labelled Cell Count 177 284 234 244 400 382 609 395 427 430 349 404 291 388 565 404 204 176 194 281 227 200 558 450


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 8.3 7.8 7.3 7.9 6.3 6.2 6.3 5.4 5.2 5.1 5.6 8.0 8.1 8.1 11.7 12.6 11.4 7.7 7.6 8.0
Std Deviation (1e-4) 1.2 1.1 1.0 1.1 1.1 1.1 1.0 1.1 1.2 1.0 0.9 0.8 1.1 1.0 1.0 1.9 1.7 1.8 1.2 1.2 1.3
Intensity Change (Log2) – – – -0.1 0.03 -0.3 -0.32 -0.3 -0.52 -0.57 -0.61 -0.47 0.05 0.07 0.06 0.6 0.7 0.56 -0.01 -0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 0.2 0.7 0.1 0 0.6 -0.1 0.6 0.6 -0.5 -0.6 0 1.0 2.7 2.4
Cytoplasm 0.9 -1.0 -3.1 -3.5 -3.9 -7.5 -6.9 -8.3 -7.9 -0.5 0.2 2.6 -2.4 -2.4 -4.1
Endoplasmic Reticulum 0 0 1.2 0 1.7 0 0 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 4.6 3.8 9.8 9.2 11.0 8.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3722 6.3466 6.0958 5.9886 5.7027 6.0607 2.3235 4.5962 3.7684 3.1739 2.8324 3.4878 4.1302 6.1252 5.4265 5.012 3.9653 5.1833
Actin 0.0305 0.001 0.0091 0.0007 0.039 0.001 0.0075 0.0026 0.0009 0.0013 0.0047 0.0009 0.0366 0.0119 0.0119 0.0071 0.0048 0.0089
Bud 0.0024 0.0003 0.0006 0.0005 0.0002 0.0004 0.0005 0.0004 0.0005 0.0196 0.0027 0.0002 0.0058 0.023 0.022 0.0094 0.0009 0.0026
Bud Neck 0.0209 0.0003 0.0004 0.0006 0.0004 0.0009 0.0007 0.0002 0.0003 0.0006 0.0016 0.0007 0.0009 0.0011 0.0014 0.0011 0.0007 0.0023
Bud Periphery 0.0018 0.0002 0.001 0.0004 0.0002 0.0014 0.0006 0.0001 0.0001 0.0148 0.0054 0.0001 0.0044 0.0103 0.0102 0.0073 0.0007 0.0017
Bud Site 0.0063 0.0037 0.0015 0.0009 0.0002 0.0002 0.0008 0.0013 0.0015 0.0011 0.0016 0.0002 0.0081 0.0605 0.0203 0.002 0.0008 0.0007
Cell Periphery 0.001 0.0015 0.0014 0.0004 0.0004 0.0008 0.0002 0.0002 0.0001 0.0004 0.0002 0 0.0007 0.0012 0.0007 0.0003 0.0002 0.0002
Cytoplasm 0.6047 0.9346 0.8677 0.6171 0.7713 0.8898 0.6843 0.9424 0.9035 0.7376 0.7124 0.8812 0.6825 0.803 0.794 0.7868 0.7132 0.7985
Cytoplasmic Foci 0.0368 0.0096 0.0164 0.0665 0.0174 0.014 0.054 0.0175 0.0205 0.0269 0.0532 0.0248 0.0448 0.0172 0.0355 0.0349 0.0357 0.0385
Eisosomes 0.0008 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0 0 0.0005 0.0001 0.0001 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0781 0.011 0.017 0.0879 0.0067 0.0185 0.0292 0.0093 0.0047 0.0039 0.0052 0.0045 0.0218 0.0031 0.0145 0.0158 0.0062 0.0128
Endosome 0.0447 0.0142 0.0281 0.1709 0.0719 0.036 0.1251 0.0133 0.0465 0.1089 0.1258 0.0485 0.035 0.0067 0.0316 0.0408 0.0651 0.0465
Golgi 0.0112 0.0024 0.0058 0.007 0.0099 0.0045 0.0226 0.0046 0.0061 0.0118 0.0163 0.0119 0.0052 0.0007 0.0053 0.0025 0.0056 0.0055
Lipid Particles 0.0219 0.0004 0.001 0.0006 0.0178 0.0001 0.0081 0.0001 0.0003 0.0027 0.0051 0.0048 0.0179 0.0005 0.0015 0.0019 0.0363 0.0058
Mitochondria 0.0105 0.0005 0.0057 0.0158 0.0015 0.0146 0.0173 0.0011 0.0015 0.0445 0.0218 0.0066 0.0355 0.0029 0.0033 0.0046 0.053 0.019
None 0.0726 0.0147 0.026 0.0078 0.0285 0.0122 0.0116 0.0027 0.0053 0.0056 0.0031 0.0009 0.0542 0.0435 0.024 0.0322 0.017 0.0299
Nuclear Periphery 0.0054 0.0006 0.0009 0.0064 0.0033 0.0007 0.0106 0.0005 0.001 0.0009 0.0027 0.0005 0.0104 0.0011 0.0025 0.013 0.0119 0.0076
Nucleolus 0.0008 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0.0001 0.0002 0 0.0012 0.0007 0.0008 0.001 0.0005 0.0016
Nucleus 0.0039 0.0006 0.0008 0.0019 0.0007 0.0006 0.0044 0.0004 0.0008 0.001 0.0016 0.0005 0.0059 0.0039 0.0081 0.01 0.0032 0.0053
Peroxisomes 0.0249 0.0001 0.0088 0.0003 0.0116 0.0001 0.0025 0.0005 0.0001 0.0027 0.0046 0.0084 0.0045 0.0009 0.0011 0.0008 0.0299 0.0013
Punctate Nuclear 0.0131 0.0001 0.0002 0.0006 0.0049 0.0001 0.0084 0.0001 0.0003 0.0002 0.0094 0.0002 0.0051 0.0018 0.0024 0.0076 0.0017 0.004
Vacuole 0.0063 0.0039 0.0063 0.0101 0.012 0.0027 0.0079 0.0022 0.0048 0.0122 0.0167 0.0037 0.0158 0.0055 0.0076 0.0165 0.0078 0.0049
Vacuole Periphery 0.0014 0.0003 0.001 0.0035 0.0017 0.0013 0.0032 0.0005 0.0011 0.0032 0.0058 0.0014 0.0034 0.0007 0.0012 0.0042 0.0045 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.6694 42.9364 35.018 36.7563 28.3317 25.5156 40.3818 41.4435 37.6858 40.7417
Translational Efficiency 1.471 1.1703 1.4153 1.6108 1.7873 1.8065 1.1032 1.1034 1.1845 1.1644

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1848 398 442 842 1686 1858 182 238 3534 2256 624 1080

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 826.28 979.60 1176.96 976.94 916.03 939.55 1159.30 1027.93 869.10 946.62 1171.81 988.18
Standard Deviation 91.41 137.07 139.05 153.41 111.10 126.35 139.14 155.27 110.76 129.21 139.31 155.27
Intensity Change Log 2 — 0.245562 0.510363 0.241639 — 0.036575 0.339787 0.166275 — 0.139466 0.423200 0.202507

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000233 0.000594 0.000642 0.000438 0.000178 0.000254 0.000371 0.000559 0.000207 0.000314 0.000563 0.000465
Bud Neck 0.003138 0.003332 0.001926 0.003629 0.001536 0.002947 0.002129 0.002175 0.002374 0.003015 0.001985 0.003309
Bud Site 0.016451 0.056143 0.019922 0.016341 0.017473 0.029301 0.012663 0.040392 0.016938 0.034037 0.017805 0.021641
Cell Periphery 0.000201 0.000183 0.000140 0.000076 0.000160 0.000113 0.000155 0.000063 0.000181 0.000125 0.000145 0.000073
Cytoplasm 0.634184* 0.585174* 0.745532* 0.439708* 0.657974* 0.622835* 0.447105* 0.602486* 0.645534* 0.616191* 0.658491* 0.475579*
Cytoplasmic Foci 0.237340* 0.238289* 0.065588 0.063950 0.221592* 0.231588* 0.103710 0.096155 0.229827* 0.232770* 0.076707 0.071047
Eisosomes 0.000062 0.000026 0.000015 0.000027 0.000025 0.000031 0.000069 0.000014 0.000045 0.000030 0.000031 0.000024
Endoplasmic Reticulum 0.006286 0.001123 0.000888 0.001688 0.008705 0.001887 0.001040 0.000230 0.007440 0.001752 0.000932 0.001366
Endosome 0.014458 0.026294 0.006886 0.016989 0.013135 0.020151 0.021070 0.011609 0.013827 0.021235 0.011023 0.015803
Golgi 0.014541 0.015625 0.000609 0.004083 0.017253 0.020416 0.000788 0.000333 0.015835 0.019570 0.000661 0.003256
Lipid Particles 0.003183 0.001604 0.001724 0.001501 0.002099 0.001484 0.003539 0.000689 0.002666 0.001505 0.002253 0.001322
Mitochondria 0.002328 0.000733 0.000166 0.000910 0.002076 0.005220 0.000734 0.000050 0.002208 0.004429 0.000332 0.000720
Mitotic Spindle 0.000254 0.005843 0.007414 0.012632 0.001072 0.002917 0.004240 0.002152 0.000644 0.003433 0.006488 0.010322
None 0.001277 0.000104 0.001794 0.001996 0.000849 0.000367 0.000519 0.001156 0.001073 0.000321 0.001422 0.001811
Nuclear Periphery 0.000220 0.000022 0.000299 0.000778 0.000124 0.000041 0.000419 0.000094 0.000174 0.000037 0.000334 0.000627
Nuclear Periphery Foci 0.000625 0.000422 0.006563 0.004018 0.000445 0.000177 0.006858 0.001583 0.000539 0.000220 0.006649 0.003481
Nucleolus 0.000208 0.000082 0.000108 0.000371 0.000183 0.000171 0.000286 0.000134 0.000196 0.000155 0.000160 0.000319
Nucleus 0.009674 0.002040 0.037068 0.037334 0.004785 0.003428 0.046255 0.008149 0.007342 0.003183 0.039748 0.030902
Peroxisomes 0.000874 0.002300 0.000493 0.001591 0.000697 0.001453 0.002044 0.001213 0.000789 0.001603 0.000945 0.001508
Vacuole 0.047286 0.051226 0.101862 0.390114* 0.036294 0.046512 0.345317* 0.229900* 0.042042 0.047343 0.172870 0.354808*
Vacuole Periphery 0.007178 0.008843 0.000361 0.001827 0.013341 0.008708 0.000688 0.000863 0.010118 0.008731 0.000457 0.001615

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.33 -2.90 -0.79 1.54 2.36 -3.59 -3.58 -1.18 -0.94 -0.47 -2.18 -3.44 -1.54 -0.63 1.96
Bud Neck -0.53 1.75 0.56 0.85 -0.53 -5.96 -2.15 -1.14 3.39 0.56 -3.07 -0.65 -0.96 0.98 -0.55
Bud Site -9.59 -0.75 1.48 9.76 1.69 -7.43 0.83 -1.77 0.22 -1.99 -11.54 -0.24 0.19 7.53 0.33
Cell Periphery 1.08 2.60 13.09 7.49 7.37 4.77 1.65 12.32 6.67 6.68 6.54 2.45 16.59 8.26 9.10
Cytoplasm 3.34 -2.47 15.70 7.75 13.80 3.97 9.17 6.55 4.84 -2.27 4.17 3.40 17.79 13.85 9.72
Cytoplasmic Foci -0.03 31.19 36.14 17.75 2.18 -1.51 16.55 18.56 19.90 1.13 -0.53 35.29 41.91 34.96 2.86
Eisosomes 8.01 11.99 10.78 0.55 -2.44 -1.87 -4.45 4.72 5.74 5.99 5.46 5.27 10.36 3.53 3.16
Endoplasmic Reticulum 6.36 6.68 6.10 0.08 -0.16 5.91 7.01 7.68 4.99 7.59 7.87 9.60 9.12 2.23 0.39
Endosome -4.25 8.52 2.26 5.01 -2.89 -6.54 -0.70 3.98 8.28 2.65 -7.64 4.83 2.98 7.85 -0.86
Golgi -0.60 28.15 10.09 6.88 -1.62 -2.81 26.97 30.37 20.86 1.11 -4.01 40.06 14.88 14.66 -1.54
Lipid Particles 2.33 3.79 7.87 0.47 4.38 2.86 -1.51 6.99 4.23 4.22 5.69 1.04 8.47 1.64 5.77
Mitochondria 4.19 5.89 3.89 0.64 -1.35 -2.87 3.26 5.96 4.98 0.99 -2.48 7.15 5.35 4.52 -0.80
Mitotic Spindle -2.23 -5.85 -4.13 -0.91 -0.41 -2.10 -1.83 -1.61 -0.72 0.69 -3.66 -5.49 -4.26 -2.63 -0.22
None 7.00 -1.76 -0.50 -2.75 0.40 2.88 1.95 0.31 -1.00 -0.53 5.27 -1.74 -0.70 -2.46 0.11
Nuclear Periphery 2.22 -3.34 -2.66 -4.82 -0.64 1.50 -4.36 -1.12 -7.19 5.00 2.53 -5.51 -3.20 -5.01 -0.18
Nuclear Periphery Foci 1.03 -11.39 -2.36 -2.55 4.17 2.48 -8.90 -6.40 -8.42 6.93 3.32 -14.34 -2.82 -3.22 5.42
Nucleolus 2.18 2.09 -1.82 -3.75 -4.30 0.10 -0.26 0.91 0.82 3.23 0.47 0.83 -1.06 -1.28 -3.18
Nucleus 15.11 -11.59 -12.98 -17.04 3.77 3.41 -5.88 -5.88 -6.94 4.66 11.03 -13.25 -14.37 -16.93 4.90
Peroxisomes -7.15 5.05 -0.79 3.71 -2.15 -8.63 -0.88 -1.07 1.84 0.48 -10.59 0.20 -1.26 2.42 -1.03
Vacuole -0.94 -12.15 -34.17 -31.27 -17.37 -4.48 -16.00 -15.11 -14.59 0.83 -2.81 -18.52 -37.51 -36.69 -12.80
Vacuole Periphery -1.29 14.04 11.15 6.58 -3.19 3.95 12.47 12.38 13.57 -0.12 1.85 17.57 15.61 13.98 -3.05
Standard name
Human Ortholog
Description Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Syh1

Syh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available