Standard name
Human Ortholog
Description Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.08 0.11 0.18 0.16 0.15 0.09 0.12 0.12 0.11 0.06 0.18 0.18 0.14 0.09 0.12 0 0.08 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0.05 0.05 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.72 0.08 0.21 0.16 0.47 0.45 0.68 0.66 0.75 0.59 0.28 0.08 0.12 0.1 0.06 0.12 0 0 0 0 0 0.05
Nucleus 0.77 0.39 0.78 0.74 0.59 0.54 0.51 0.48 0.41 0.43 0.52 0.75 0.69 0.64 0.63 0.75 0.69 0.54 0.45 0.49 0.42 0.38 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.16 0.07 0.16 0.15 0.16 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.19 0.13 0.12 0.06 0.08 0.05 0 0 0.07 0 0 0 0.08 0 0.06 0.07 0.1 0.06 0.08 0.1 0.12
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.09 0.07 0.08 0.2 0.14 0.2 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 3
Bud 0 1 0 0 2 3 8 12 19 9 8 1 0 0 1 1 0 3 1 2 4 16 14
Bud Neck 0 1 6 2 7 2 9 2 10 6 8 1 0 0 0 1 0 2 2 3 3 9 18
Bud Site 0 1 0 0 0 4 3 18 15 8 15 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 7 13 17 19 51 41 55 36 52 31 38 19 60 70 26 12 16 3 16 5 3 5 6
Endoplasmic Reticulum 2 1 0 0 0 0 0 1 0 0 0 0 1 2 4 5 4 0 0 1 1 2 2
Endosome 1 1 1 0 4 4 3 5 0 0 3 1 12 23 11 7 7 11 1 3 7 26 18
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 2 0 0 3 0 3 0
Mitochondria 53 135 17 37 46 122 163 261 295 199 201 95 27 45 18 8 16 7 9 8 8 18 21
Nucleus 145 74 173 128 164 141 185 186 182 114 179 253 231 244 114 97 90 130 94 144 97 179 139
Nuclear Periphery 1 1 0 0 0 0 0 0 0 4 0 0 0 1 1 0 0 1 0 0 0 3 2
Nucleolus 6 6 0 1 1 1 3 6 9 4 4 6 8 23 6 1 4 37 14 46 33 77 67
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 5 0 0 0 0 0 0 0 1 0
SpindlePole 8 4 43 23 34 15 30 21 20 11 24 13 11 11 15 5 8 17 19 18 19 48 54
Vac/Vac Membrane 2 6 3 2 11 4 7 11 13 5 8 3 11 8 21 11 9 19 40 41 45 71 84
Unique Cell Count 189 188 221 174 279 259 364 385 449 267 343 337 337 380 182 129 131 242 208 293 231 476 448
Labelled Cell Count 225 244 260 212 321 337 466 559 616 391 489 394 361 433 220 148 156 242 208 293 231 476 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.4 4.9 4.7 5.0 4.2 4.2 3.9 3.7 3.4 3.8 4.3 4.4 4.7 6.6 6.8 6.6 4.1 4.3 4.6
Std Deviation (1e-4) 0.6 0.6 1.2 1.6 1.4 1.7 1.2 1.6 1.4 1.3 0.9 0.8 0.9 4.4 1.6 1.5 1.5 1.2 1.0 1.3
Intensity Change (Log2) -0.08 0.02 -0.24 -0.25 -0.36 -0.42 -0.52 -0.37 -0.19 -0.18 -0.06 0.41 0.46 0.43 -0.26 -0.21 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 4.3 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1972 -0.4278 -0.815 -0.5875 0.189 -0.7521 -0.3745 -0.2213 -0.2747 -0.5055 -0.4192 -0.4983 -0.5947 -0.3201 -0.7614 -0.7331 -0.6871 -0.8668
Actin 0.0258 0.0007 0.0017 0.0292 0.0615 0.0019 0.0659 0.004 0.0217 0.0119 0.0016 0.0048 0.0087 0.0002 0.0095 0.0002 0.0004 0.0173
Bud 0.0002 0.0004 0.0002 0.0005 0.0021 0.0001 0.0014 0.0002 0.0007 0.0003 0.0002 0.0003 0.0002 0.0002 0.0004 0.0001 0.0006 0.0001
Bud Neck 0.0013 0.0025 0.0014 0.0011 0.0022 0.0253 0.0217 0.001 0.0065 0.0017 0.0023 0.0032 0.0013 0.0011 0.0008 0.0009 0.0026 0.0047
Bud Periphery 0.0002 0.0006 0.0002 0.0015 0.0049 0.0003 0.0042 0.0001 0.0009 0.0002 0.0003 0.0008 0.0005 0.0001 0.0009 0.0001 0.0005 0.0002
Bud Site 0.0015 0.0079 0.0016 0.0003 0.0061 0.0005 0.0089 0.0028 0.0067 0.0055 0.0004 0.0006 0.001 0.0014 0.0017 0.0003 0.0016 0.0004
Cell Periphery 0.0001 0.0003 0.0001 0.0001 0.0006 0.0001 0.0011 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0002
Cytoplasm 0.0252 0.0556 0.0288 0.004 0.0249 0.0319 0.0347 0.0529 0.0212 0.0365 0.0168 0.0167 0.0445 0.0644 0.0241 0.0168 0.0345 0.0125
Cytoplasmic Foci 0.0152 0.0052 0.0102 0.0009 0.0583 0.0031 0.0138 0.0123 0.0083 0.0382 0.0056 0.0104 0.0135 0.0026 0.0034 0.0008 0.0073 0.0027
Eisosomes 0.0002 0.0001 0.0001 0.0002 0.0007 0.0001 0.0005 0 0.0002 0 0 0.0001 0.0002 0 0.0001 0 0 0.0013
Endoplasmic Reticulum 0.0023 0.0028 0.0015 0.0094 0.0044 0.0015 0.0065 0.0014 0.0011 0.0013 0.0013 0.0006 0.0068 0.0018 0.0015 0.0006 0.0021 0.0018
Endosome 0.0177 0.0046 0.0093 0.0273 0.0322 0.0068 0.0298 0.0055 0.0088 0.0173 0.0468 0.0062 0.0324 0.0024 0.0049 0.0008 0.0437 0.0025
Golgi 0.0042 0.0004 0.0011 0.009 0.0161 0.0025 0.0049 0.0032 0.0088 0.0208 0.0353 0.0044 0.0316 0.0001 0.0023 0 0.0012 0.0038
Lipid Particles 0.0273 0.0016 0.0091 0.003 0.0599 0.0107 0.0043 0.0007 0.0057 0.0073 0.0166 0.0043 0.0363 0.0013 0.0026 0.0001 0.0008 0.0083
Mitochondria 0.0025 0.0008 0.0018 0.0052 0.0772 0.0009 0.0041 0.001 0.026 0.0014 0.018 0.0043 0.0068 0.0005 0.0167 0.0004 0.0016 0.0018
None 0.1525 0.1159 0.09 0.0015 0.1003 0.0564 0.0294 0.0797 0.0283 0.0433 0.0659 0.014 0.043 0.092 0.013 0.0157 0.0058 0.0261
Nuclear Periphery 0.0336 0.0181 0.0207 0.0307 0.0488 0.0222 0.0336 0.0145 0.0214 0.0208 0.0442 0.0075 0.0381 0.0167 0.0163 0.012 0.0263 0.011
Nucleolus 0.0124 0.0144 0.0189 0.0084 0.0077 0.0325 0.0115 0.0178 0.0179 0.0036 0.0074 0.0197 0.0135 0.0122 0.0152 0.0074 0.0185 0.0264
Nucleus 0.5806 0.6535 0.763 0.8542 0.3748 0.6969 0.6553 0.7239 0.764 0.7612 0.6669 0.8365 0.6471 0.7499 0.8086 0.8911 0.7745 0.7848
Peroxisomes 0.0118 0.0009 0.0024 0.0004 0.0302 0.0008 0.0012 0.0057 0.0055 0.0122 0.0015 0.0131 0.0028 0.0001 0.0031 0 0.0006 0.0064
Punctate Nuclear 0.0823 0.1068 0.0356 0.0087 0.0665 0.1043 0.0606 0.0717 0.0437 0.0156 0.0614 0.0515 0.0671 0.0514 0.07 0.052 0.0566 0.0869
Vacuole 0.0016 0.0064 0.0017 0.0017 0.0105 0.0008 0.0043 0.0013 0.0015 0.0005 0.0048 0.0006 0.0028 0.0014 0.0035 0.0004 0.0171 0.0006
Vacuole Periphery 0.0014 0.0006 0.0005 0.0025 0.0101 0.0006 0.0022 0.0002 0.001 0.0003 0.0026 0.0004 0.0017 0.0003 0.0012 0.0001 0.0037 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0309 5.2677 6.189 6.6176 11.7785 4.4171 7.7673 5.542 6.8803 11.7743
Translational Efficiency 0.4759 1.0179 0.3896 0.4142 0.4596 1.0949 0.5411 0.476 0.4253 0.3791

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2277 191 383 1244 2091 1529 1929 1814 4368 1720 2312 3058

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.77 787.11 912.12 848.40 695.80 720.74 805.23 860.35 671.28 728.11 822.94 855.49
Standard Deviation 66.23 111.60 108.24 117.94 81.13 110.77 93.16 106.29 77.39 112.81 103.74 111.33
Intensity Change Log 2 0.278858 0.491517 0.387038 0.050806 0.210728 0.306251 0.165348 0.353039 0.345796

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.001349 0.001960 0.001862 0.000136 0.000548 0.000414 0.000991 0.000099 0.000637 0.000670 0.001345
Bud Neck 0.003763 0.011572 0.012748 0.023478 0.004994 0.006334 0.007437 0.025692 0.004352 0.006915 0.008317 0.024791
Bud Site 0.000867 0.007389 0.003120 0.025703 0.000777 0.001579 0.004025 0.012193 0.000824 0.002224 0.003875 0.017689
Cell Periphery 0.000093 0.000154 0.000145 0.000235 0.000089 0.000115 0.000192 0.000107 0.000091 0.000120 0.000184 0.000159
Cytoplasm 0.151592* 0.065551 0.014697 0.081890 0.078630 0.052620 0.071337 0.039579 0.116665* 0.054056 0.061954 0.056791
Cytoplasmic Foci 0.023247 0.021524 0.000451 0.006348 0.020054 0.023803 0.003205 0.001263 0.021719 0.023550 0.002749 0.003331
Eisosomes 0.000031 0.000075 0.000061 0.000054 0.000035 0.000058 0.000037 0.000031 0.000033 0.000060 0.000041 0.000040
Endoplasmic Reticulum 0.000742 0.006902 0.010876 0.009313 0.001410 0.001997 0.002011 0.007178 0.001062 0.002542 0.003479 0.008047
Endosome 0.000782 0.004809 0.000872 0.008423 0.000912 0.003976 0.000910 0.001713 0.000844 0.004069 0.000904 0.004443
Golgi 0.000258 0.000925 0.000186 0.001860 0.000754 0.003361 0.000151 0.000269 0.000495 0.003090 0.000157 0.000916
Lipid Particles 0.003992 0.005295 0.000859 0.002011 0.005375 0.008864 0.000781 0.000653 0.004654 0.008468 0.000794 0.001206
Mitochondria 0.004037 0.005360 0.001624 0.006983 0.007101 0.019003 0.001925 0.004047 0.005504 0.017488 0.001875 0.005241
Mitotic Spindle 0.000739 0.019202 0.007402 0.051884 0.004121 0.024272 0.009083 0.025040 0.002358 0.023709 0.008804 0.035960
None 0.019065 0.013582 0.006103 0.011150 0.015446 0.014128 0.020014 0.008255 0.017332 0.014067 0.017709 0.009433
Nuclear Periphery 0.002097 0.001854 0.001106 0.002965 0.002798 0.002921 0.001051 0.002609 0.002432 0.002803 0.001060 0.002754
Nuclear Periphery Foci 0.001035 0.003005 0.000735 0.004226 0.001183 0.001905 0.000381 0.001086 0.001106 0.002027 0.000440 0.002363
Nucleolus 0.019202 0.024792 0.012505 0.007483 0.027748 0.040579 0.013177 0.008551 0.023293 0.038826 0.013065 0.008116
Nucleus 0.765247* 0.789131* 0.916789* 0.724610* 0.823285* 0.782334* 0.858334* 0.846831* 0.793030* 0.783089* 0.868018* 0.797111*
Peroxisomes 0.001684 0.004894 0.000578 0.005530 0.003295 0.007382 0.000787 0.000598 0.002455 0.007105 0.000753 0.002604
Vacuole 0.001154 0.008782 0.006944 0.021596 0.001509 0.003010 0.004559 0.011839 0.001324 0.003651 0.004954 0.015808
Vacuole Periphery 0.000307 0.003852 0.000239 0.002396 0.000347 0.001212 0.000190 0.001475 0.000326 0.001505 0.000199 0.001849

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.85 -11.91 -14.32 -0.45 2.88 -3.06 -12.37 -11.94 -4.03 -5.86 -4.76 -17.06 -18.66 -5.57 -5.78
Bud Neck -4.11 -10.60 -14.90 -6.69 -4.50 -2.16 -8.84 -18.13 -16.54 -12.95 -4.60 -13.37 -23.65 -19.56 -14.55
Bud Site -2.56 -6.93 -8.52 -2.84 -6.17 -3.31 -4.23 -7.54 -6.67 -3.98 -3.76 -5.16 -11.30 -9.37 -7.02
Cell Periphery -2.87 -3.65 -11.02 -1.09 -2.40 -3.20 -5.73 -2.89 1.09 4.04 -4.22 -6.78 -9.81 -2.36 0.85
Cytoplasm 8.53 29.40 17.93 1.08 -10.27 6.16 6.55 12.94 5.76 5.84 15.89 19.91 23.23 4.01 1.58
Cytoplasmic Foci 0.44 16.55 14.12 3.63 -5.94 -1.51 12.98 14.05 13.64 3.93 -0.82 19.97 20.27 13.63 0.49
Eisosomes -5.45 -8.15 -12.88 1.62 0.81 -7.65 -2.45 -1.52 6.88 1.18 -10.83 -7.24 -10.56 3.86 -1.76
Endoplasmic Reticulum -5.46 -14.54 -24.41 -3.80 0.10 -4.64 -11.59 -23.50 -20.75 -15.98 -8.77 -18.85 -33.89 -23.74 -16.12
Endosome -2.01 -2.00 -3.95 0.28 -3.50 -4.15 -0.12 -1.77 3.61 -1.79 -4.67 -1.25 -4.34 2.13 -4.01
Golgi -2.59 0.71 -2.62 0.68 -2.92 -3.45 4.12 3.72 4.35 -1.77 -3.98 4.30 0.72 4.11 -3.42
Lipid Particles -0.91 10.26 8.42 3.24 -2.40 -3.68 10.11 10.57 11.69 2.32 -5.13 14.38 13.95 11.72 -0.81
Mitochondria -0.95 3.35 -0.86 0.30 -4.21 -5.68 5.69 3.69 8.14 -5.41 -6.80 6.41 2.25 7.86 -6.23
Mitotic Spindle -2.71 -2.37 -10.67 -2.22 -7.83 -6.24 -3.77 -8.70 -1.26 -5.56 -7.53 -5.51 -13.97 -2.97 -8.90
None 2.80 11.45 9.13 2.35 -2.64 0.69 0.82 7.56 7.44 5.45 2.79 3.80 12.11 7.85 4.92
Nuclear Periphery 0.16 2.04 -9.12 -7.45 -11.14 -0.29 6.74 -4.29 -2.69 -11.10 -1.12 6.77 -9.31 -4.37 -16.15
Nuclear Periphery Foci -1.54 0.83 -2.28 0.54 -2.83 -2.04 4.03 1.68 3.39 -2.20 -2.95 3.63 -1.20 1.66 -3.76
Nucleolus -1.51 3.08 8.11 5.14 2.23 -4.38 6.73 10.64 13.29 3.65 -6.85 6.16 13.03 14.67 4.83
Nucleus -0.82 -16.95 0.08 0.83 14.07 4.59 -8.10 -3.50 -7.45 4.36 1.80 -16.22 -3.55 -4.25 11.29
Peroxisomes -2.69 4.43 -0.15 2.41 -2.15 -3.77 4.99 5.45 8.52 0.69 -5.60 6.03 4.22 7.90 -1.53
Vacuole -4.16 -6.54 -13.37 -8.04 -8.67 -3.05 -7.53 -10.85 -9.66 -6.11 -5.40 -9.77 -17.10 -14.86 -11.36
Vacuole Periphery -1.13 0.16 -2.78 0.85 -3.11 -2.98 3.32 -1.61 1.34 -2.39 -2.82 1.94 -2.98 1.38 -3.92
Standard name
Human Ortholog
Description Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Nto1

Nto1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nto1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available